{"id":9220,"date":"2017-03-14T11:37:48","date_gmt":"2017-03-14T03:37:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9220"},"modified":"2022-07-22T15:15:22","modified_gmt":"2022-07-22T07:15:22","slug":"wst-1%e6%b0%b4%e6%ba%b6%e6%80%a7%e5%9b%9b%e5%94%91%e9%b9%bd-%e7%b4%b0%e8%83%9e%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90biovision%e8%b2%a8%e8%99%9f2198","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9220","title":{"rendered":"WST-1\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | \u7d30\u80de\u6d3b\u6027\u5206\u6790Biovision\u8ca8\u865f#2198"},"content":{"rendered":"

WST-1\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | <\/strong>\u8ca8\u865f<\/strong>2<\/strong>198<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u898f\u683c: <\/strong>WST-1\u662f\u4e00\u7a2e\u6c34\u6eb6\u6027 tetrazolium \u8a66\u5291\uff0c\u7528\u4f5cNADH\u7684\u654f\u611f\u986f\u8272\u6307\u793a\u5291\u3002\u5b83\u5728\u4e2d\u6027pH\u4e0b\u57281-\u7532\u6c27\u57faPMS\u5b58\u5728\u4e0b\u88abNADH\u9084\u539f\uff0c\u7522\u751f\u5728438nM\u5438\u6536\u7684colored formazan dye\u53ef\u7528\u65bc\u7d30\u80de\u589e\u6b96\u6e2c\u5b9a\u4f5c\u70ba\u7d30\u80de\u6d3b\u529b\u6307\u793a\u5291\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1<\/strong>\u4ecb\u7d39<\/strong>:<\/strong> WST\u662f\u4e00\u7a2e\u985e\u4f3c\u65bcMTT\u7684\u5316\u5408\u7269\uff0c\u5728\u96fb\u5b50\u8026\u5408\u8a66\u5291\u5b58\u5728\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5\u88ab\u7dda\u7c92\u9ad4\u5167\u7684\u4e00\u4e9b\u812b\u6c2b\u9176\u9084\u539f\u751f\u6210\u6a59\u9ec3\u8272\u7684formazan\u3002\u7d30\u80de\u589e\u6b96\u8d8a\u591a\u8d8a\u5feb\uff0c\u5247\u984f\u8272\u8d8a\u6df1\uff1bWST\u662fMTT\u7684\u4e00\u7a2e\u5347\u7d1a\u66ff\u4ee3\u7522\u54c1\uff0c\u548cMTT\u6216\u5176\u5b83MTT\u985e\u4f3c\u7522\u54c1\u5982XTT\u3001MTS\u7b49\u76f8\u6bd4\u6709\u660e\u986f\u7684\u512a\u9ede\uff1bMTT\u88ab\u7dda\u7c92\u9ad4\u5167\u7684\u4e00\u4e9b\u812b\u6c2b\u9176\u9084\u539f\u751f\u6210\u7684formazan\u4e0d\u662f\u6c34\u6eb6\u6027\u7684\uff0c\u9700\u8981\u6709\u7279\u5b9a\u7684\u6eb6\u89e3\u6db2\u4f86\u6eb6\u89e3\uff1b\u800cWST\u548cXTT\u3001MTS\u7522\u751f\u7684formazan\u90fd\u662f\u6c34\u6eb6\u6027\u7684\uff0c\u53ef\u4ee5\u7701\u53bb\u5f8c\u7e8c\u7684\u6eb6\u89e3\u6b65\u9a5f\u3002WST\u7522\u751f\u7684formazan\u6bd4XTT\u548cMTS\u7522\u751f\u7684formazan\u66f4\u6613\u6eb6\u89e3\u3002\u518d\u6b21\uff0cWST\u6bd4XTT\u548cMTS\u66f4\u52a0\u7a69\u5b9a\uff0c\u4f7f\u5be6\u9a57\u7d50\u679c\u66f4\u52a0\u7a69\u5b9a\u3001\u4ea6\u5177\u6709\u7dda\u6027\u7bc4\u570d\u66f4\u5bec\uff0c\u9748\u654f\u5ea6\u66f4\u9ad8\u4e4b\u7279\u9ede\u3002\u53ef\u4ee5\u7528\u65bc\u7d30\u80de\u56e0\u6578\u7b49\u8a98\u5c0e\u7684\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\uff0c\u4e5f\u53ef\u4ee5\u7528\u65bc\u6297\u764c\u85e5\u7269\u7b49\u5c0d\u7d30\u80de\u6709\u6bd2\u8a66\u5291\u8a98\u5c0e\u7684\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\uff0c\u6216\u4e00\u4e9b\u85e5\u7269\u8a98\u5c0e\u7684\u7d30\u80de\u751f\u9577\u6291\u5236\u6aa2\u6e2c\u7b49\u3002\u4f7f\u7528WST\u6642\u7121\u9808\u540c\u4f4d\u7d20\uff0c\u6240\u6709\u7684\u6aa2\u6e2c\u6b65\u9a5f\u50c5\u572896well\u5b8c\u6210\u3002\u4e0d\u5fc5\u6d17\u6ecc\u7d30\u80de\uff0c\u4e0d\u5fc5\u6536\u96c6\u7d30\u80de\uff0c\u4e5f\u4e0d\u5fc5\u63a1\u7528\u984d\u5916\u6b65\u9a5f\u53bb\u6eb6\u89e3formazan\u3002 WST\u5c0d\u7d30\u80de\u7121\u660e\u986f\u6bd2\u6027\u3002\u52a0\u5165WST\u986f\u8272\u5f8c\uff0c\u53ef\u4ee5\u5728\u4e0d\u540c\u6642\u9593\u53cd\u5fa9\u7528\u9176\u6a19\u5100\u8b80\u677f\uff0c\u4f7f\u6aa2\u6e2c\u6642\u9593\u66f4\u52a0\u9748\u6d3b\uff0c\u4fbf\u65bc\u627e\u5230\u6700\u4f73\u6e2c\u5b9a\u6642\u9593\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\n2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nPale brown solid<\/td>\n<\/tr>\n
CAS #<\/strong><\/td>\n150849-52-8<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC\u2081\u2089H\u2081\u2081IN\u2085NaO\u2088S\u2082<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n651.35<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n\u226597% by HPLC<\/td>\n<\/tr>\n
Solubility<\/strong><\/td>\nH2O (10 mg\/ml); 6.5 mg\/ 10ml 50mM Tris buffer, pH 8.0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u66f4\u591a\u9ad8\u9748\u654f\u5ea6\u7d30\u80de\u6d3b\u6027\u87a2\u8272\u5206\u6790\u5957\u7d44 | \u76f8\u95dc\u5206\u6790<\/strong><\/p>\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K305-1000<\/td>\n\u00a0EZViable\u2122 Calcein AM Cell Viability Assay Kit (Fluorometric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K502-100<\/td>\n\u00a0Live\/Dead Cell Viability Assay Kit (for Mammalian Cells)<\/a><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K303-2500<\/td>\n\u00a0VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K303-500<\/td>\n\u00a0VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7d30\u80de\u6d3b\u6027\u5206\u6790\u76f8\u95dc\u7522\u54c1<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K306-200<\/td>\n\u00a0BrdU Cell Proliferation Assay Kit<\/a><\/td>\n\u00a0200 assays<\/td>\n<\/tr>\n
\u00a0K306-1000<\/td>\n\u00a0BrdU Cell Proliferation Assay Kit<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K300-500<\/td>\n\u00a0MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K300-2500<\/td>\n\u00a0MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K299-1000<\/td>\n\u00a0MTT Cell Proliferation Assay Kit (Colorimetric)<\/a><\/td>\n\u00a01000 assays<\/td>\n<\/tr>\n
\u00a0K302-2500<\/td>\n\u00a0WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K302-500<\/td>\n\u00a0WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n
\u00a0K301-2500<\/td>\n\u00a0WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a02500 assays<\/td>\n<\/tr>\n
\u00a0K301-500<\/td>\n\u00a0WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u7d30\u80de\u6d3b\u6027\u5206\u6790\u76f8\u95dc\u5546\u54c1<\/strong><\/p>\n

Biovision\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u7d30\u80de\u5b58\u6d3b\u5075\u6e2c\u5206\u6790\u5957\u7d44\u3002WST-1 Cell Proliferation Colorimetric Assay Kit<\/strong>\u5feb\u901f\u7d30\u80de\u589e\u6b96\u5206\u6790\u5957\u7d44\uff0c\u4f7f\u7528\u6c34\u6eb6\u6027tetrazolium\u9e7d\u985e\u7684\u7684WST-1\u4f5c\u70ba\u67d3\u5291\uff0c\u5229\u7528\u7c92\u817a\u9ad4\u4e2d\u7684\u812b\u6c2b\u9176\u9084\u539f\u6210\u6a59\u8272\u7522\u7269\u3002WST-1\u6bd4 \u8d77\u50b3\u7d71\u7684MTT\u3001XTT\u6216\u662fMTS-base\u5206\u6790\u8981\u4f86\u7684\u66f4\u5feb\u901f\u4e14\u9748\u654f\u5ea6\u66f4\u9ad8\u3002Senescence Detection Kit<\/strong>\uff1a \u7d30\u80de\u8001\u5316\u88ab\u8a8d\u70ba\u662f\u6291\u5236\u764c\u7d30\u80de\u7684\u6a5f\u5236\uff0c\u56e0\u6b64\u7576\u7d30\u80de\u8001\u5316\u6642\u96d6\u7136\u7d30\u80de\u6703\u6301\u7e8c\u5b58\u6d3b\uff0c\u4f46\u662f\u5728\u9019\u500b\u968e\u6bb5\u7684\u7d30\u80de\u662f\u4e0d\u6703\u5206\u88c2\uff0c\u4e5f\u7121\u6cd5\u4ee5\u8840\u6e05\u53ca\u57f9\u990a\u57fa\u9032\u884c\u7d30\u80de\u7e6b\u4ee3\u57f9\u990a\u3002Senescence detection kit\u662f\u5075\u6e2c\u5207\u7247\u4e2d\u7d44\u7e54\u6216\u7d30\u80de\u4e0a \u7279\u5b9a\u7684senescence marker\uff0c\u800c\u9019\u4e9bmarker\u662f\u4e0d\u6703\u51fa\u73fe\u5728\u5176\u4ed6\u7684\u7d30\u80de\u4e2d\u3002Bioluminescence Cytotoxicity Assay Kit<\/strong>\uff1a \u9019\u5957\u7d30\u80de\u6bd2\u6bba\u8a66\u5291\u7d44\u662f\u900f\u904e\u53d7\u640d\u50b7\u7684\u7d30\u80de\u91cb\u653eAdenylate Kinase (AK)\uff0c\u5229\u7528AK\u5c07phosphorylates ADP \u8f49\u5316\u70ba ATP\uff0c\u7522\u751f\u7684 ATP \u80fd\u5920\u53c3\u8207 Luciferase \u51b7\u5149\u53cd\u61c9\uff0c\u518d\u900f\u904e\u51b7\u5149\u5075\u6e2c\u5100\u5075\u6e2c\u7d50\u679c\uff0c\u6bcf\u6b21\u5206\u6790\u53ea\u8981 10 \u500b\u4ee5\u4e0a\u7d30\u80de\u5c31\u80fd\u5206\u6790\uff0c\u800c\u4e14\u53ea\u898110\u5206\u5c31\u80fd\u5f97\u5230\u7d50\u679c\u3002 LDH-Cytotoxicity Assay Kit<\/strong>\uff1a \u9019\u500b\u8a66\u5291\u7d44\u662f\u900f\u904e\u5075\u6e2c\u4e73\u9178\u812b\u6c2b\u9176(lactate dehydrogenase, LDH)\u6d3b\u6027\u5224\u65b7\u7d30\u80de\u6bd2\u6bba\u73fe\u8c61\u3002LDH\u5b58\u5728\u65bc\u6240 \u6709\u7d30\u80de\u4e2d\uff0c\u7576\u7d30\u80de\u7834\u88c2\u6642\u624d\u6703\u91cb\u653e\u81f3\u7d30\u80de\u5916\u3002\u4f7f\u7528LDH-cytotoxicity assay kit\u53ea\u9700\u8981\u5206\u5149\u5149\u5ea6\u8a08500nm \u6ce2\u9577\u7684\u5340\u6bb5\uff0c\u76f8\u7576\u9069\u5408\u4e00\u822c\u5be6\u9a57\u5ba4\u4f7f\u7528\u3002 Live-Dead Cell Staining Kit<\/strong>\uff1a Live-Dead Cell Staining Kit\u662fready-to-use\u7684\u8a66\u5291\uff0c\u88e1\u9762\u542b2\u7a2e\u4e0d\u540c\u6ce2\u9577\u7684\u67d3\u5291\uff0c\u5305\u62ec\u67d3\u6d3b\u7d30\u80de\u7684Live -dye (\u7da0\u87a2\u5149\uff0cEx\/Em = 490\/515 nm)\u4ee5\u53ca\u67d3\u6b7b\u7d30\u80de\u7684propidium iodide (PI\uff0c\u7d05\u87a2\u5149\uff0cEx\/Em = 488\/615 nm)\uff0c\u7d30\u80de\u7d93\u904e\u67d3\u8272\u5f8c\uff0c\u80fd\u5920\u76f4\u63a5\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u7d30\u80de\u5b58\u6d3b\u7684\u72c0\u6cc1\u3002High Throughput Cell Viability<\/strong> Assays\uff1a ApoSENSORTM Cell Viability Assay\u662f\u4f7f\u7528\u51b7\u5149\u65b9\u5f0f\u5075\u6e2cATP\u91cf\uff0c\u4e26\u5224\u65b7 \u7d30\u80de\u662f\u5426\u5b58\u6d3b\u6216\u9032\u884c\u7d30\u80de\u51cb\u4ea1\u3002ApoSENSORTM ADP\/ATP Ratio Kit\u5247\u662f\u5229 \u7528ADP\/ATP\u6bd4\u4f8b\u8b8a\u5316\u5224\u65b7\u7d30\u80de\u5728\u51cb\u4ea1\u3001\u58de\u6b7b\u6216\u662f\u589e\u6b96\u3002<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

WST-1\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | \u8ca8\u865f2198 \u7522\u54c1\u898f\u683c: WST-1\u662f\u4e00\u7a2e\u6c34\u6eb6\u6027 tetrazolium \u8a66\u5291\uff0c\u7528 […]<\/p>\n","protected":false},"author":3,"featured_media":9221,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[123,180],"tags":[3884],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9220"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9220"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9220\/revisions"}],"predecessor-version":[{"id":14448,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9220\/revisions\/14448"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9221"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9220"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9220"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9220"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}