{"id":9214,"date":"2017-03-14T11:25:44","date_gmt":"2017-03-14T03:25:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=9214"},"modified":"2022-07-22T15:15:29","modified_gmt":"2022-07-22T07:15:29","slug":"wst-3%e6%b0%b4%e6%ba%b6%e6%80%a7%e5%9b%9b%e5%94%91%e9%b9%bd-%e8%b2%a8%e8%99%9f2814","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=9214","title":{"rendered":"WST-3\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | \u7d30\u80de\u6d3b\u6027\u5206\u6790Biovision\u8ca8\u865f2814"},"content":{"rendered":"<p><strong>\u5546\u54c1: WST-3\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | <\/strong><strong>\u8ca8\u865f<\/strong><strong>2814-100 \u00a0(100mg\/500mg\/1g)<\/strong><\/p>\n<p><strong>\u7522\u54c1\u898f\u683c: <\/strong>WST-3\u662f\u4e00\u7a2e\u5ee3\u6cdb\u61c9\u7528\u65bc\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u7684\u5feb\u901f\u9ad8\u9748\u654f\u5ea6\u6aa2\u6e2c\u8a66\u5291\uff1bBiovision\u65b0\u4e00\u4ee3WST-3\u5177\u6709\u78fa\u9178\u57fa\uff0c\u5b83\u5011\u76f4\u63a5\u6216\u9593\u63a5\u5730\u52a0\u5165\u82ef\u74b0\u4ee5\u589e\u9032\u6c34\u6eb6\u6027\u3002WST-3\u662f\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\u7684\u56db\u5511\u9393\u8a66\u5291\uff0c\u7528\u9084\u539f\u5291\u5982NADH\u7522\u751f\u6c34\u6eb6\u6027\u6709\u8272colored formazan dye\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.biovision.com\/media\/catalog\/product\/cache\/1\/image\/650x\/040ec09b1e35df139433887a97daa66f\/2\/8\/2814.gif\" alt=\"\" width=\"274\" height=\"280\" \/><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u4ecb\u7d39<\/strong><strong>:<\/strong> WST-3\u662f\u4e00\u7a2e\u985e\u4f3c\u65bcMTT\u7684\u5316\u5408\u7269\uff0c\u5728\u96fb\u5b50\u8026\u5408\u8a66\u5291\u5b58\u5728\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5\u88ab\u7dda\u7c92\u9ad4\u5167\u7684\u4e00\u4e9b\u812b\u6c2b\u9176\u9084\u539f\u751f\u6210\u6a59\u9ec3\u8272\u7684formazan\u3002\u7d30\u80de\u589e\u6b96\u8d8a\u591a\u8d8a\u5feb\uff0c\u5247\u984f\u8272\u8d8a\u6df1\uff1bWST\u662fMTT\u7684\u4e00\u7a2e\u5347\u7d1a\u66ff\u4ee3\u7522\u54c1\uff0c\u548cMTT\u6216\u5176\u5b83MTT\u985e\u4f3c\u7522\u54c1\u5982XTT\u3001MTS\u7b49\u76f8\u6bd4\u6709\u660e\u986f\u7684\u512a\u9ede\uff1bMTT\u88ab\u7dda\u7c92\u9ad4\u5167\u7684\u4e00\u4e9b\u812b\u6c2b\u9176\u9084\u539f\u751f\u6210\u7684formazan\u4e0d\u662f\u6c34\u6eb6\u6027\u7684\uff0c\u9700\u8981\u6709\u7279\u5b9a\u7684\u6eb6\u89e3\u6db2\u4f86\u6eb6\u89e3\uff1b\u800cWST\u548cXTT\u3001MTS\u7522\u751f\u7684formazan\u90fd\u662f\u6c34\u6eb6\u6027\u7684\uff0c\u53ef\u4ee5\u7701\u53bb\u5f8c\u7e8c\u7684\u6eb6\u89e3\u6b65\u9a5f\u3002WST\u7522\u751f\u7684formazan\u6bd4XTT\u548cMTS\u7522\u751f\u7684formazan\u66f4\u6613\u6eb6\u89e3\u3002\u518d\u6b21\uff0cWST\u6bd4XTT\u548cMTS\u66f4\u52a0\u7a69\u5b9a\uff0c\u4f7f\u5be6\u9a57\u7d50\u679c\u66f4\u52a0\u7a69\u5b9a\u3001\u4ea6\u5177\u6709\u7dda\u6027\u7bc4\u570d\u66f4\u5bec\uff0c\u9748\u654f\u5ea6\u66f4\u9ad8\u4e4b\u7279\u9ede\u3002\u53ef\u4ee5\u7528\u65bc\u7d30\u80de\u56e0\u6578\u7b49\u8a98\u5c0e\u7684\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\uff0c\u4e5f\u53ef\u4ee5\u7528\u65bc\u6297\u764c\u85e5\u7269\u7b49\u5c0d\u7d30\u80de\u6709\u6bd2\u8a66\u5291\u8a98\u5c0e\u7684\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\uff0c\u6216\u4e00\u4e9b\u85e5\u7269\u8a98\u5c0e\u7684\u7d30\u80de\u751f\u9577\u6291\u5236\u6aa2\u6e2c\u7b49\u3002\u4f7f\u7528WST\u6642\u7121\u9808\u540c\u4f4d\u7d20\uff0c\u6240\u6709\u7684\u6aa2\u6e2c\u6b65\u9a5f\u50c5\u572896well\u5b8c\u6210\u3002\u4e0d\u5fc5\u6d17\u6ecc\u7d30\u80de\uff0c\u4e0d\u5fc5\u6536\u96c6\u7d30\u80de\uff0c\u4e5f\u4e0d\u5fc5\u63a1\u7528\u984d\u5916\u6b65\u9a5f\u53bb\u6eb6\u89e3formazan\u3002 WST\u5c0d\u7d30\u80de\u7121\u660e\u986f\u6bd2\u6027\u3002\u52a0\u5165WST\u986f\u8272\u5f8c\uff0c\u53ef\u4ee5\u5728\u4e0d\u540c\u6642\u9593\u53cd\u5fa9\u7528\u9176\u6a19\u5100\u8b80\u677f\uff0c\u4f7f\u6aa2\u6e2c\u6642\u9593\u66f4\u52a0\u9748\u6d3b\uff0c\u4fbf\u65bc\u627e\u5230\u6700\u4f73\u6e2c\u5b9a\u6642\u9593\u3002<\/p>\n<table id=\"product-attribute-specs-table\" class=\"data-table\">\n<tbody>\n<tr class=\"first odd\">\n<th class=\"label\">Alternate Name<\/th>\n<td class=\"data last\">4-[3-(4-Iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate<\/td>\n<\/tr>\n<tr class=\"even\">\n<th class=\"label\">Appearance<\/th>\n<td class=\"data last\">Pale yellow to pale brown solid<\/td>\n<\/tr>\n<tr class=\"odd\">\n<th class=\"label\">CAS #<\/th>\n<td class=\"data last\">515111-36-1<\/td>\n<\/tr>\n<tr class=\"even\">\n<th class=\"label\">Molecular Formula<\/th>\n<td class=\"data last\">C\u2081\u2089H\u2081\u2080IN\u2086NaO\u2081\u2080S\u2082<\/td>\n<\/tr>\n<tr class=\"odd\">\n<th class=\"label\">Molecular Weight<\/th>\n<td class=\"data last\">696.34<\/td>\n<\/tr>\n<tr class=\"even\">\n<th class=\"label\">Purity<\/th>\n<td class=\"data last\">\u226598% by HPLC<\/td>\n<\/tr>\n<tr class=\"odd\">\n<th class=\"label\">Solubility<\/th>\n<td class=\"data last\">H\u2082O (10 mg\/ml)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u66f4\u591a\u9ad8\u9748\u654f\u5ea6\u7d30\u80de\u6d3b\u6027\u87a2\u8272\u5206\u6790\u5957\u7d44 | \u76f8\u95dc\u5206\u6790<\/strong><\/p>\n<table width=\"73%\">\n<tbody>\n<tr>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/fluorescence-cell-viablity-assays-2856\/index-1-1a.html\">Catalog #<\/a><\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/fluorescence-cell-viablity-assays-2856\/index-1-2d.html\">Product Name+<\/a><\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/fluorescence-cell-viablity-assays-2856\/index-1-3a.html\">Size<\/a><\/td>\n<\/tr>\n<tr>\n<td>\u00a0K305-1000<\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/ezviable-calcein-am-cell-viability-assay-kit-fluorometric-8491.html\">EZViable\u2122 Calcein AM Cell Viability Assay Kit (Fluorometric)<\/a><\/td>\n<td>\u00a01000 assays<\/td>\n<\/tr>\n<tr>\n<td>\u00a0K502-100<\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/live-dead-cell-viability-assay-kit-for-mammalian-cells-10139.html\">Live\/Dead Cell Viability Assay Kit (for Mammalian Cells)<\/a><\/td>\n<td>\u00a0100 assays<\/td>\n<\/tr>\n<tr>\n<td>\u00a0K303-2500<\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/visionblue-quick-cell-viability-fluorometric-assay-kit-2841.html\">VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n<td>\u00a02500 assays<\/td>\n<\/tr>\n<tr>\n<td>\u00a0K303-500<\/td>\n<td>\u00a0<a href=\"http:\/\/www.biovision.com\/visionblue-quick-cell-viability-fluorometric-assay-kit-2842.html\">VisionBlue\u2122 Quick Cell Viability Fluorometric Assay Kit<\/a><\/td>\n<td>\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u7d30\u80de\u6d3b\u6027\u5206\u6790\u76f8\u95dc\u7522\u54c1<\/strong><\/p>\n<table style=\"height: 582px;\" width=\"697\">\n<tbody>\n<tr>\n<td width=\"15%\">\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/colorimetric-cell-proliferation-assays-2855\/index-1-1a.html\">Catalog #<\/a><\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/colorimetric-cell-proliferation-assays-2855\/index-1-2d.html\">Product Name+<\/a><\/td>\n<td width=\"17%\">\u00a0<a href=\"http:\/\/www.biovision.com\/cancer-research-2608\/cell-proliferation-viability-cytotoxicity-2852\/colorimetric-cell-proliferation-assays-2855\/index-1-3a.html\">Size<\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K306-200<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/brdu-cell-proliferation-assay-kit-8818.html\">BrdU Cell Proliferation Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a0200 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K306-1000<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/brdu-cell-proliferation-assay-kit-8819.html\">BrdU Cell Proliferation Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a01000 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K300-500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/mts-cell-proliferation-colorimetric-assay-kit-8078.html\">MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a0500 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K300-2500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/mts-cell-proliferation-colorimetric-assay-kit-8079.html\">MTS Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a02500 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K299-1000<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/mtt-cell-proliferation-assay-kit-colorimetric-9649.html\">MTT Cell Proliferation Assay Kit (Colorimetric)<\/a><\/td>\n<td width=\"17%\">\u00a01000 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K302-2500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/wst-cell-proliferation-colorimetric-assay-kit-plus-3595.html\">WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n<td width=\"17%\">\u00a02500 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K302-500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/wst-cell-proliferation-colorimetric-assay-kit-plus-3596.html\">WST Cell Proliferation Colorimetric Assay Kit plus<\/a><\/td>\n<td width=\"17%\">\u00a0500 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K301-2500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/wst-1-cell-proliferation-colorimetric-assay-kit-2837.html\">WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a02500 assays<\/td>\n<\/tr>\n<tr>\n<td width=\"15%\">\u00a0K301-500<\/td>\n<td width=\"66%\">\u00a0<a href=\"http:\/\/www.biovision.com\/wst-1-cell-proliferation-colorimetric-assay-kit-2838.html\">WST-1 Cell Proliferation Colorimetric Assay Kit<\/a><\/td>\n<td width=\"17%\">\u00a0500 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5546\u54c1: WST-3\u6c34\u6eb6\u6027\u56db\u5511\u9e7d | \u8ca8\u865f2814-100 \u00a0(100mg\/500mg\/1g) \u7522\u54c1\u898f\u683c: WS [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":9216,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[123,180],"tags":[3885],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9214"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9214"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9214\/revisions"}],"predecessor-version":[{"id":9219,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/9214\/revisions\/9219"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/9216"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9214"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9214"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9214"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}