{"id":8876,"date":"2017-02-03T11:29:07","date_gmt":"2017-02-03T03:29:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8876"},"modified":"2022-07-22T15:59:25","modified_gmt":"2022-07-22T07:59:25","slug":"pck2-lentiviral-vector%e8%a1%a8%e7%8f%be%e8%bc%89%e9%ab%94-%e8%b2%a8%e8%99%9flv257336","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=8876","title":{"rendered":"PCK2 Lentiviral Vector\u8868\u73fe\u8f09\u9ad4 | \u8ca8\u865fLV257336"},"content":{"rendered":"

\u5546\u54c1<\/strong>:<\/strong> PCK2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) \u00a0| <\/strong>\u8ca8\u865f<\/strong>LV257336<\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> PCK2\u6162\u75c5\u6bd2\u8868\u9054\u8f09\u9ad4DNA\uff1bPCK2\u86cb\u767d\u8868\u9054\u7531CMV\u555f\u52d5\u5b50\u9a45\u52d5\uff0c\u4e0d\u5177\u4efb\u4f55\u6a19\u7c64Tag\u6216\u5831\u544a\u7269reporter\u8868\u73fe\uff1bDNA\u8f09\u9ad4\u53ef\u7531NheI\u548cXhoI\u5207\u4f4d\uff0c\u67b6\u69cb\u65bc\u5176\u5b83\u8868\u73fe\u7cfb\u5217\u4e0a\u3002\u73fe\u6210\u4e4bPCK2 Lentiviral Vector\u53ef\u76f4\u63a5transfection\u8f49\u67d3\uff0c\u7acb\u5373\u8868\u9054\u975e\u75c5\u6bd2\u8cea\u7c92\u86cb\u767d\uff1b\u6216\u5229\u7528\u5305\u88dd\u7cfb\u5217\u8868\u9054\u6210\u6162\u75c5\u6bd2\u9846\u7c92\uff0c\u5177\u9ad8\u6548\u7387\u8f49\u5c0etransduction\u548c\u7a69\u5b9aintegrated\u6574\u5408\u8868\u9054\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Insert<\/strong><\/td>\nPCK2<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nBC001454<\/a><\/td>\n<\/tr>\n
Vector<\/strong><\/td>\npLenti-GIII-CMV<\/a><\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n8074bp<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\n1923 bp<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Tags<\/strong><\/td>\nNo Tags<\/td>\n<\/tr>\n
Promoter<\/strong><\/td>\nCMV<\/td>\n<\/tr>\n
Selection Marker<\/strong><\/td>\nBacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nLentiviral Plasmid<\/td>\n<\/tr>\n
Viral Packaging<\/strong><\/td>\nThe suggested packaging mix for this vector is Cat#\u00a0LV003, 2nd Generation Packaging Mix<\/a>.
\nAlternatively, you can also use Cat#\u00a0LV053, 3rd Generation Packaging Mix<\/a><\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Stability<\/strong><\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/span>\u00a0Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5546\u54c1: PCK2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-C […]<\/p>\n","protected":false},"author":3,"featured_media":8880,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[456],"tags":[3959],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8876"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8876"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8876\/revisions"}],"predecessor-version":[{"id":8888,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8876\/revisions\/8888"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8880"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8876"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8876"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8876"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}