{"id":8824,"date":"2017-01-23T14:53:24","date_gmt":"2017-01-23T06:53:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8824"},"modified":"2022-07-22T16:01:20","modified_gmt":"2022-07-22T08:01:20","slug":"%e8%a1%80%e6%b6%b2%e7%9b%b4%e6%8e%a5pcr-bloodirect-2x-pcr-mastermix%e8%b2%a8%e8%99%9fg465","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=8824","title":{"rendered":"\u8840\u6db2\u76f4\u63a5PCR | Bloodirect 2X PCR MasterMix\u8ca8\u865fG465"},"content":{"rendered":"

\u76f8\u95dc\u5546\u54c1<\/span>\u00a0\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1<\/a>\u00a0| Agarose |\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a>\u00a0|\u00a0\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a>\u00a0|\u00a0CMOS\u87a2\u5149\u76f8\u6a5f<\/a>\u00a0|Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a><\/span><\/strong><\/p>\n

\u8840\u6db2\u76f4\u63a5<\/strong>PCR-<\/strong>Bloodirect 2X PCR MasterMix | <\/strong>\u8ca8\u865f<\/strong>G465<\/strong><\/a><\/span><\/p>\n

\u53ef\u76f4\u63a5\u61c9\u7528\u65bc\u5168\u8840\u3001\u51b7\u51cd\u8840\u6db2\u3001Guthrie\u5361\u6216903\u5361\u3001FTA\u5361\u7b49\u8840\u6db2\u6aa2\u9ad4<\/p>\n

\"\"<\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217PCR\/RT-PCR\/QPCR\u7cfb\u5217\u5546\u54c1\uff1b\u53ef\u61c9\u7528\u65bcDNA\/RNA\u5206\u5b50\u5c64\u9762\u4e4b\u7814\u7a76\uff1b\u5176\u4e2dBloodirect 2X PCR MasterMix(G465)<\/u> , \u53ef\u6392\u9664\u8840\u6db2\u4e2dEDTA\u3001\u6ab8\u6aac\u9178\u9e7d\u6216\u809d\u7d20\u4e4b\u5e72\u64fe\uff0c\u53ef\u61c9\u7528\u65bc\u8840\u6db2\u6aa2\u9ad4\uff0c\u76f4\u63a5\u9032\u884cPCR\u4e4b\u53cd\u61c9\u3002\u9069\u7528\u65bc\u65b0\u9bae\u6216\u51b7\u51cd\u904e\u8840\u6db2\u3002\u6b64\u7522\u54c1\u512a\u9ede\u76f4\u63a5\u7c21\u5316\u8840\u6db2PCR\u4e4b\u5de5\u4f5c\u6d41\u7a0b\uff1b\u4ee5\u9810\u6df7\u548c\u512a\u5316\u7684\u5f62\u5f0f\u63d0\u4f9b\u4e86PCR\u6240\u9700\u7684\u6240\u6709\u6210\u5206\u3002\u5229\u7528\u4e0d\u540c\u7684Taq DNA\u805a\u5408\u9176\uff0c\u63d0\u4f9b\u4e86\u591a\u7a2e\u9ad8\u5ea6\u9748\u654f\u7684MasterMix\uff0c\u4ee5\u9069\u61c9\u5404\u7a2eDNA\u6a21\u677f\u548c\u6027\u80fd\u4e4b\u9700\u6c42\u3002\u53e6\u5916MasterMix\u4e2d\u5177\u6709blue dye\u548c\u7a69\u5b9a\u5291\u6599\uff0c\u5141\u8a31PCR\u7522\u7269\u76f4\u63a5\u52a0\u8f09\u5230\u74ca\u8102\u7cd6\u51dd\u81a0\u4e0a\uff0c\u4e0d\u9700\u518d\u984d\u5916\u6dfb\u52a0Loading dye\u3002<\/p>\n

\"\"\"\"<\/p>\n

Bloodirect 2X PCR MasterMix<\/strong>\u4f7f\u7528\u9808\u77e5<\/strong><\/p>\n

\u5efa\u8b70\u53ef\u4f7f\u7528\u65bc\u542b\u6709\u6ab8\u6aac\u9178\u9209\uff0cEDTA\u9209\uff0cEDTA\u9240\u6216\u809d\u7d20\u9209\u7684\u5168\u8840\u6a23\u54c1\u3002\u8840\u6db2\u9ad4\u7a4d\u82e5\u9ad8\u65bc10\uff05\uff08v \/ v\uff09\uff0c\u6703\u589e\u52a0\u5f9ePCR\u5f8c\u5269\u9918\u7684\u8840\u6db2\u7d30\u80de\u788e\u7247\u4e2d\u56de\u6536\u542b\u6c34\u4e0a\u6e05\u6db2\u7684\u56f0\u96e3\u5ea6\u3002Dry blood s\u8840\u6db2\u82e5\u5b58\u5132\u65bcGuthrie\u5361\u6216903\u5361\uff08Whatman\uff0cNJ\uff09\u4e0a\uff1b\u53ef\u4ee5\u76f4\u63a5\u52a0\u51651 mm punch disc\u76f4\u63a5\u65bcPCR\u53cd\u61c9\u4e2d\u3002\u5c0d\u65bc\u5b58\u5132\u5728FTA\u5361\uff08Whatman\uff0cNJ\uff09\u4e0a\u7684\u8840\u6db2\uff0c\u9996\u5148\u53d61 mm punch disc\u572850\u03bcl\u6c34\u4e2d\u572855\u2103\u4e0b\u5b75\u80b25\u5206\u9418\uff0c\u7136\u5f8cdiscard\u6c34\u5f8c\uff0c\u5c07disc\u52a0\u5165PCR\u4e2d\u76f4\u63a5\u53cd\u61c9\u3002\u91dd\u5c0d\u9ad8GC rich\u4e4b\u76ee\u6a19\uff0c\u5efa\u8b70\u4f7f\u752810\uff05\uff08v \/ v\uff09\u7684DMSO\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u76f8\u95dc\u5546\u54c1\u00a0\u5b89\u5168\u6838\u9178\u67d3\u5291\u5546\u54c1\u00a0| Agarose |\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20\u00a0|\u00a0\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44\u00a0| […]<\/p>\n","protected":false},"author":3,"featured_media":8825,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[132],"tags":[3972],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8824"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8824"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8824\/revisions"}],"predecessor-version":[{"id":32276,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8824\/revisions\/32276"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/8825"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8824"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8824"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8824"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}