{"id":8233,"date":"2016-12-22T13:41:27","date_gmt":"2016-12-22T05:41:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8233"},"modified":"2022-07-22T16:51:17","modified_gmt":"2022-07-22T08:51:17","slug":"tunel-dna%e7%89%87%e6%ae%b5%e5%81%b5%e6%b8%ac-apo-brdu-dna-fragmentation-assay-kit%e8%b2%a8%e8%99%9fk401-60","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=8233","title":{"rendered":"(Green) TUNEL DNA\u7247\u6bb5\u5075\u6e2c| Apo-BrdU DNA Fragmentation Assay Kit\u8ca8\u865fK401-60"},"content":{"rendered":"

TUNEL-<\/strong>\u87a2\u5149<\/strong>F<\/strong>low<\/strong>\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u5546\u54c1<\/strong> (<\/strong>\u7da0\u87a2\u5149<\/strong>)<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K401-60<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> \u6838\u5c0f\u9ad4\u9593DNA\u7247\u6bb5\u5316\u662f\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u51cb\u4ea1\u7684\u6a19\u8a8c\u3002 BioVision\u7684Apo-BrdU\u539f\u4f4dDNA\u7247\u6bb5\u5316\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\uff0c\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u65b9\u4fbf\u5730\u6aa2\u6e2cDNA\u7247\u6bb5\u5316\u3002 \u8a72\u8a66\u5291\u76d2\u4f7f\u7528Br-dUTP\uff08\u6eb4\u5316\u812b\u6c27\u5c3f\u82f7\u4e09\u78f7\u9178\u6838\u82f7\u9178\uff09\uff0c\u5176\u6bd4\u5176\u4ed6\u8f03\u5927\u914d\u9ad4\uff08\u4f8b\u5982\u87a2\u5149\u7d20\uff0c\u751f\u7269\u7d20\u6216\u6d0b\u5730\u9ec3\u6bd2\u82f7\uff09\u66f4\u5bb9\u6613\u647b\u5165DNA\u93c8\u65b7\u88c2\u4e2d\u3002 \u7576\u901a\u904e\u87a2\u5149\u7d20\u6a19\u8a18\u7684\u6297BrdU\u55ae\u514b\u9686\u6297\u9ad4\u9451\u5b9aBr-dUTP\u4f4d\u9ede\u6642\uff0c\u7522\u751f\u66f4\u4eae\u7684\u4fe1\u865f\u3002Apo-BrdU DNA Fragmentation Assay Kit\u5546\u54c1\u8a2d\u8a08\u539f\u7406BrdU-FITC\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c\u51cb\u4ea1\u7d30\u80de\u4e2d\u7684DNA\u93c8\u65b7\u88c2\u3002BrdU\u63d0\u4f9b\u5feb\u901f\u65b9\u4fbf\u4e4bTUNEL\u5075\u6e2c\uff0c\u7c21\u55ae\u6d41\u7a0b\u53ea\u97003\u5c0f\u6642\u5b8c\u6210\u3002\u65bcFlow cytometry\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf\u4e2d\uff0c\u5176\u9748\u654f\u5ea6\u9ad8\u65bc\u751f\u7269\u7d20\u5316\u6216\u5730\u9ad8\u8f9b\u5316\u3002\u5957\u7d44\u5167\u63d0\u4f9b\u96d9\u8272\u6aa2\u6e2c\uff1b\u53ef\u61c9\u7528\u6d41\u5f0f\u7d30\u80de\u8853\uff08Ex \/ Em = FITC\u7684488 \/ 520nm\uff0cPI\u7684488 \/ 623nm\uff09\u6216\u87a2\u5149\u986f\u5fae\u93e1\uff08FITC\u548cRhodamine\uff09\u96d9\u8272\u6aa2\u6e2c\u3002\u53ef\u61c9\u7528\u65bc\u54fa\u4e73\u52d5\u7269\u4e4b\u7d30\u80de\u57f9\u990a\uff08\u7c98\u9644\u548c\u61f8\u6d6e\uff09\u6216\u77f3\u881f\u5305\u57cb\u7d44\u7e54\u5207\u7247\u3002\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\u6216\u8f09\u73bb\u7247\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u00a0<\/strong>DNA\u7247\u6bb5\u5316\u70ba\u7d30\u80de\u51cb\u4ea1\u7684\u5176\u4e2d\u4e00\u9805\u7279\u5fb5\u3002\u7d30\u80de\u51cb\u4ea1\u6d3b\u5316\u5167\u751f\u6027\u7684\u5167\u5207\u9176\uff0c\u63a5\u8457\u5728\u6838\u5167\u5c07\u67d3\u8272\u8ceaDNA\u5207\u65b7\u6210\u4ee5180 bp\u70ba\u500d\u6578\u7684\u5c0f\u7247\u6bb5\uff08360\u3001540 etc.\uff09\u3002\u9019\u500b\u73fe\u8c61\u53ef\u4ee5\u88ab\u4f8b\u5982DNA laddering assay\u3001TUNEL assay\u3001\u6216Nicoletti assay\u7528\u4f86\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3002TUNEL assay\uff0c\u5168\u540d\u70baTerminal deoxynucleotidyl transferase (TdT) dUTP nick end labeling assay\u3002\u7d30\u80de\u5728\u53d7\u5230\u5167\u5207\u9176\u9020\u6210\u7684DNA\u65b7\u88c2\u5f8c\uff0c\u5229\u7528TdT\u50ac\u5316\u4f7fBrdUTP\u80fd\u8207\u65b7\u88c2\u8655\u4e4b3\u2019-OH\u9032\u884c\u53cd\u61c9\uff0c\u9032\u800c\u5c07\u5e36\u6709\u87a2\u5149\u6a19\u5b9a\uff08\u5982\uff1aFITC\u7b49\uff09\u7684BrdU\u6297\u9ad4\u8207BrdUTP\u7d50\u5408\uff0c\u89c0\u5bdf\u5176\u87a2\u5149\u5206\u4f48\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK401-60<\/td>\n<\/tr>\n
Size<\/th>\n60 assays<\/td>\n<\/tr>\n
Detection Method<\/th>\nFlow cytometry (Ex\/Em = 488\/520 nm for FITC, and 488\/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\nThe TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.<\/td>\n<\/tr>\n
Features & Benefits<\/th>\n\u2022 Simple procedure; takes ~ 3 hours
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive and stable
\n\u2022 The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 Positive Control Cells
\n\u2022 Negative Control Cells
\n\u2022 Wash Buffer
\n\u2022 Reaction Buffer
\n\u2022 TdT Enzymes
\n\u2022 Br-dUTP
\n\u2022 Rinse Buffer
\n\u2022 Anti-BrdU-FITC Antibody
\n\u2022 PI\/RNase Staining Buffer<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

\n
Kuo, Jinn-Rung et al. (2016) Cisplatin-induced regulation of signal transduction pathways and transcription factors in p53-mutated subclone variants of hepatoma cells: Potential application for therapeutic targeting,\u00a0Oncol Lett. 2016 Nov;12(5):3723-3730.<\/a><\/div>\n<\/div>\n
\n
Malik, Salma et al. (2016) Therapeutic Potential and Molecular Mechanisms of Emblica officinalis Gaertn in Countering Nephrotoxicity in Rats Induced by the Chemotherapeutic Agent Cisplatin,\u00a0Front Pharmacol. 2016 Oct 3;7:350.<\/a><\/div>\n<\/div>\n
\n
Shang, Hung-Sheng et al. (2016) Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells,\u00a0Oncol Rep. 2016 Oct;36(4):2207-15.<\/a><\/div>\n<\/div>\n
\n
Bharti, Brij et al. (2016) Level of PAX5 in differential diagnosis of non-Hodgkin’s lymphoma,\u00a0Indian J Med Res. 2016 May;143(Supplement):S23-S31.<\/a><\/div>\n<\/div>\n
\n
Sun et al., Change of Recipient Corneal Endothelial Cells After Non-Descemet’s Stripping Automated Endothelial Keratoplasty in a Rabbit Model.\u00a0Invest. Ophthalmol. Vis. Sci.,\u00a0Dec 2014; 55: 8467 – 8474.<\/a><\/div>\n<\/div>\n

\"\u672a\u547d\u540d-14\"\u00a0\u5feb\u901f<\/strong>TUNEL<\/strong>\u87a2\u5149<\/strong>F<\/strong>low<\/strong>\u5206\u6790\u53ca\u87a2\u5149\u986f\u5fae\u93e1\u5546\u54c1<\/strong> (<\/strong>\u7d05\u87a2\u5149<\/strong>)<\/strong> | <\/strong>\u8ca8\u865f<\/strong>K404-60 <\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0<\/strong>:<\/strong> Apo-BrdU-Red\u2122 DNA Fragmentation Assay Kit<\/strong>\u5546\u54c1\u8a2d\u8a08\u539f\u7406BrdU-RED\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c\u51cb\u4ea1\u7d30\u80de\u4e2d\u7684DNA\u93c8\u65b7\u88c2\u3002BrdU\u4ee5\u7d05\u5149\u6a19\u5b9a\uff0c\u5c08\u9580\u8a2d\u8a08\u7528\u65bc\u542bGFP\u7684\u7d30\u80de\uff0c\u4ee5\u63d0\u4f9b\u4e0d\u540c\u87a2\u5149\u4e4bTUNEL\u5206\u6790\u65b9\u6cd5\u3002 Flow cytometry (Ex\/Em = 488\/576 nm for BrdU-Red and 488\/655 nm for 7-AAD). \u5957\u7d44\u5167\u63d0\u4f9b\u96d9\u8272\u6aa2\u6e2c\uff1b\u53ef\u61c9\u7528\u6d41\u5f0f\u7d30\u80de\u8853BrdU-Red\uff08Ex \/ Em =488\/576 nm\uff0c7-AAD\u7684488\/655 nm\uff09\u6216\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u6e2c\u3002\u53ef\u61c9\u7528\u65bc\u7d44\u7e54\u5207\u7247\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de\u3002\u8a66\u5291\u76d2\u5167\u63d0\u4f9b\u5b8c\u6574\u7684\u7d44\u4ef6\uff0c\u5305\u62ec\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7d30\u80de\uff0c\u65b9\u4fbf\u61c9\u7528\u65bc\u57f9\u990a\u7d30\u80de\u548c\u7d44\u7e54\u5207\u7247\u4e2d\uff0c\u5224\u8b80DNA\u7247\u6bb5\u3002
\n