{"id":8175,"date":"2016-12-19T13:33:28","date_gmt":"2016-12-19T05:33:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8175"},"modified":"2022-07-22T16:53:14","modified_gmt":"2022-07-22T08:53:14","slug":"%e5%bf%ab%e9%80%9f%e8%9b%8b%e7%99%bd%e8%b3%aa%e8%90%83%e5%8f%96%e8%a9%a6%e5%8a%91-mammalian-protein-extraction-reagent%e8%b2%a8%e8%99%9f8002-500","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=8175","title":{"rendered":"\u5feb\u901f\u86cb\u767d\u8cea\u8403\u53d6\u8a66\u5291 |Mammalian Protein Extraction Reagent\u8ca8\u865f8002-500"},"content":{"rendered":"

\u00a0<\/strong>\u5feb\u901f\u86cb\u767d\u8cea\u8403\u53d6\u8a66\u5291<\/strong>EZLys\u2122 Mammalian Protein Extraction Reagent |\u8ca8\u865f8002-500<\/strong><\/a><\/span><\/p>\n

\u7522\u54c1\u63cf\u8ff0: BioVision\u7684EZLysTM\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u63d0\u53d6\u8a66\u5291\uff1b\u53ef\u4ee5\u8f15\u9b06\u63d0\u53d6\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e4b\u7e3d\u86cb\u767d\u3002\u8a72\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u63d0\u53d6\u8a66\u5291\uff0c\u5177\u6709\u512a\u5316\u4e4b\u7d30\u80de\u63d0\u53d6\u7de9\u885d\u6db2\uff0c\u65b9\u4fbf\u63d0\u53d6\u4f86\u81ea\u7c98\u9644\u548c\u61f8\u6d6e\u7d30\u80de\u7684\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u3002\u5176\u7de9\u885d\u6db2\u4e4b\u6027\u8cea\u6eab\u548c\uff0c\u53ef\u5728\u975e\u8b8a\u6027\u689d\u4ef6\u4e0b\uff0c\u5feb\u901f\u5b8c\u6210\u7e3d\u86cb\u767d\u4e4b\u8403\u53d6\uff1b\u5728\u4e0d\u523020\u5206\u9418\u5167\u3002\u7d30\u80de\u88c2\u89e3\u6db2\u4f7f\u7528\u9019\u7a2e\u7de9\u885d\u6db2\u88fd\u5099\u7684\u5316\u5408\u7269\u53ef\u4ee5\u7528\u65bc\u4e0b\u6e38\u591a\u7a2e\u5be6\u9a57\u4e4b\u61c9\u7528\uff0c\u4f8b\u5982\u9176\u6d3b\u6027\u6e2c\u5b9a\uff08\u4f8b\u5982\u80f1\u5929\u86cb\u767d\u9176\u6d3b\u6027\u6e2c\u5b9acaspase activity assays\uff09\u3001\u86cb\u767d\u8cea\u5370\u8de1Western blot\u5206\u6790\u3001reporter assays\u5831\u5c0e\u5206\u6790\u3001immunoprecipitation\u514d\u75ab\u6c89\u6fb1\u7b49\u3002\u7522\u54c1\u4fdd\u5b58\u65bcStore buffer at 4\u00b0C\uff1b\u907f\u514d\u9577\u671f\u5149\u7167\u3002\u4f7f\u7528\u524d\u8acb\u8a73\u7d30\u95b1\u8b80\u64cd\u4f5c\u624b\u518a\u3002\u5176\u4ed6\u642d\u914d\u4e4b\u7522\u54c1: DNase I, protease inhibitor cocktail (Cat # K272)<\/p>\n

\"\"<\/p>\n

\u5feb\u901f\u86cb\u767d\u8cea\u8403\u53d6\u8a66\u5291\u64cd\u4f5c\u65b9\u6cd5<\/strong>Protocol:<\/strong><\/p>\n

\u57284\u2103 600xg\u96e2\u5fc35\u5206\u9418\u6536\u96c6\u7d30\u80de\u3002\u5c0d\u65bc\u8cbc\u58c1\u7d30\u80de\uff0c\u5148\u5c07\u7d30\u80de\u522e\u5165PBS\u4e2d\u4e26\u5982\u4e0a\u8ff0\u6c89\u6fb1\u7d30\u80de\u3002\u6bcf\u514bcell pellet\uff0c\u4f7f\u752810ml\u9810\u51b7\u4e4bEZLysTM\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u3002 Pipet up and down\u5438\u79fb\u4e0a\u4e0b\u5e7e\u500b\u6b21\u3002Incubate \u51b0\u4e0a20\u5206\u9418\u3002\u4ee54\u2103 10000xg\u96e2\u5fc315\u5206\u9418\u3002 \u6536\u96c6\u6f84\u6e05\u7684\u4e0a\u6e05\u6db2\u7528\u65bc\u5206\u6790\u3002 \u9577\u671f\u5132\u5b58\uff0c\u9700\u5c07\u86cb\u767d\u8cea\u5206\u7ba1\u8caf\u5b58\uff1b\u5728\u6db2\u6c2e\u4e2d\u5feb\u901f\u51b7\u51cd\u4e26\u5132\u5b58\u5728-70\u2103\u3002\u6ce8\u610f\u4e8b\u9805:<\/strong> <\/span>\u66f4\u6fc3\u7e2e\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u7269\uff0c\u6bcf\u514b\u7d30\u80depellet\u4f7f\u75285ml EZLysTM\u54fa\u4e73\u52d5\u7269\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u3002\u70ba\u4e86\u907f\u514d\u86cb\u767d\u8cea\u964d\u89e3\uff0c\u6211\u5011\u5efa\u8b70\u60a8\u6dfb\u52a0\u86cb\u767d\u9176\u6291\u88fd\u5291\u6df7\u5408\u7269\uff08Cat\uff03K272\uff09\u3002\u9ad8\u63d0\u53d6\u7269\u7c98\u5ea6\u53ef\u4ee5\u901a\u904e\u6bcf\u500b\u63d0\u53d6\u8a66\u5291\u4e2d\u52a0\u516510-15U\u7684DNAse I\u4f86\u89e3\u6c7a\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K6570-3<\/td>\n\u00a0EZExtract\u2122 Polyubiquitin Buffer Kit<\/a><\/strong><\/td>\n\u00a03 components<\/td>\n<\/tr>\n
\u00a08001-100<\/td>\n\u00a0EZLys\u2122 Bacterial Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0100 ml<\/td>\n<\/tr>\n
\u00a08001-500<\/td>\n\u00a0EZLys\u2122 Bacterial Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0500 ml<\/td>\n<\/tr>\n
\u00a08005-1G<\/td>\n\u00a0EZLys\u2122 Lysozyme, Human<\/a><\/strong><\/td>\n\u00a01 g<\/td>\n<\/tr>\n
\u00a08005-5G<\/td>\n\u00a0EZLys\u2122 Lysozyme, Human<\/a><\/strong><\/td>\n\u00a05 g<\/td>\n<\/tr>\n
\u00a08004-100<\/td>\n\u00a0EZLys\u2122 Mammalian Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0100 ml<\/td>\n<\/tr>\n
\u00a08004-500<\/td>\n\u00a0EZLys\u2122 Mammalian Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0500 ml<\/td>\n<\/tr>\n
\u00a08002-100<\/td>\n\u00a0EZLys\u2122 Tissue Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0100 ml<\/td>\n<\/tr>\n
\u00a08002-500<\/td>\n\u00a0EZLys\u2122 Tissue Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0500 ml<\/td>\n<\/tr>\n
\u00a08003-100<\/td>\n\u00a0EZLys\u2122 Yeast Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0100 ml<\/td>\n<\/tr>\n
\u00a08003-500<\/td>\n\u00a0EZLys\u2122 Yeast Protein Extraction Reagent<\/a><\/strong><\/td>\n\u00a0500 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0\u5feb\u901f\u86cb\u767d\u8cea\u8403\u53d6\u8a66\u5291EZLys\u2122 Mammalian Protein Extraction Reagent | […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[89,134],"tags":[4084],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8175"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8175"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8175\/revisions"}],"predecessor-version":[{"id":32481,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8175\/revisions\/32481"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8175"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8175"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}