{"id":8050,"date":"2016-12-08T16:45:43","date_gmt":"2016-12-08T08:45:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=8050"},"modified":"2022-12-10T12:20:54","modified_gmt":"2022-12-10T04:20:54","slug":"crispr-%e5%96%ae%e6%a0%aaclone%e7%b4%b0%e8%83%9e-%e6%9c%80%e7%b0%a1%e5%96%aegdna%e8%90%83%e5%8f%96%e6%96%b9%e6%b3%95-viagen%e8%b2%a8%e8%99%9f-302-c","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=8050","title":{"rendered":"CRISPR \u55ae\u682aclone\u7d30\u80de | \u6700\u7c21\u55aegDNA\u8403\u53d6\u65b9\u6cd5 VIAGEN\u8ca8\u865f# 302-C"},"content":{"rendered":"

HOT!! CRISPR \u55ae\u682aclone\u7d30\u80de | \u6700\u7c21\u55aegDNA\u8403\u53d6\u65b9\u6cd5 \u00a0<\/span><\/strong><\/span>DirectPCR Lysis Reagent (Cell ) \u00a0\u8ca8\u865f# 302-c 100m l\u5305\u88dd<\/span><\/p>\n

Direct PCR Lysis reagent\u00a0\u53ea\u8981\u4e00\u500b\u52a0\u71b1\u6b65\u9a5f\u53cd\u61c9\uff0c\u5373\u53ef\u5c07DNA\u7531\u7d30\u80de\u4e2d\u91cb\u653e\u3002\u6b64\u7522\u54c1\u5177\u6709\u6291\u5236PCR\u6291\u5236\u7269\u7684\u53cd\u61c9\uff0c\u56e0\u6b64\u4e0d\u9808\u8003\u91cfPCR\u6291\u5236\u554f\u984c\uff0c\u7d30\u80de\u52a0\u5165reagent\u53cd\u61c9\u5f8c\uff0c\u5373\u53ef\u9032\u884cPCR\u53cd\u61c9\u3002\u4e0d\u9808\u7d93\u7531\u4efb\u4f55\u7d14\u5316\u7684\u6b65\u9a5f\uff1b\u7d30\u80de\u52a0\u71b1\u5b8c\u5f8c\u7684\u6eb6\u6db2\u53ef\u4ee5\u76f4\u63a5PCR\uff0c\u8b93genotyping\u5206\u6790\u66f4\u5bb9\u6613\u3002\u53ef\u5feb\u901f\u61c9\u7528\u65bcCRISPR \u00a0KI \u7d30\u80de\u4e4b\u55ae\u682aclone gDNA\u88fd\u5099\u3002<\/p>\n

\"\"<\/p>\n

DirectPCR\u00ae system offers advantages over conventional protocols that include:<\/p>\n

\u00b7\u00a0Time saving:<\/strong> Less hands-on time. Crude tail lysates for PCR.
\n\u00b7\u00a0Money saving: <\/strong>Cost-effective reactions.
\n\u00b7\u00a0Safe:<\/strong> No organic reagents.
\n\u00b7\u00a0Environmental:\u00a0<\/strong>Less\u00a0waste (organic reagents, tubes, tips, etc…)
\n\u00b7\u00a0Reliable and efficient:<\/strong> Virtually 100% success rate with high yields.<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> VIAGEN BIOTECH\u63d0\u4f9b\u4e00\u7cfb\u5217\u55ae\u7ba1gDNA\u8403\u53d6\u5957\u7d44\uff0c\u4e00\u7ba1\u4e0b\u5b8c\u6210\u53cd\u61c9\uff1b\u7522\u54c1\u5206\u70ba\u5c3e\u5df4genomic DNA\u8403\u53d6\u3001\u7d30\u80degenomic DNA\u8403\u53d6\u3001\u8033\u6735genomic DNA\u8403\u53d6\u3001\u5375\u9ec3\u56cagenomic DNA\u8403\u53d6\u3002\u50b3\u7d71genomic DNA\u8403\u53d6\uff0c\u90fd\u9808\u7d93\u7531Phenol\/Chloroform\u8403\u53d6\u6b65\u9a5f\uff0c\u6216\u8005\u5e02\u552egenomic DNA Purification\u5957\u7d44(\u901a\u904ecolumn\u7b49\u7e41\u7463\u6b65\u9a5f)\u4f86\u7d14\u5316genomic DNA\u3002\u50b3\u7d71\u8403\u53d6genomic DNA\uff0c\u7121\u6cd5\u76f4\u63a5PCR\uff1b\u56e0\u7d44\u7e54\u5167\u542b\u6709PCR\u53cd\u61c9\u6291\u5236\u56e0\u5b50\uff0c\u6291\u5236PCR\u53cd\u61c9\u3002\u9808\u7d93\u7531\u6709\u6a5f\u6eb6\u5291\u6216kit\u8403\u53d6gDNA\u3002DirectPCR Lysis Reagents\u5177\u6709\u6291\u5236PCR\u6291\u5236\u7269\u7684\u53cd\u61c9\uff0c\u4e0d\u9808\u8003\u91cfPCR\u6291\u5236\u554f\u984c\u3002VIAGEN BIOTECH\u516c\u53f8\u5c08\u9580\u4ee5DirectPCR Lysis Reagent\u92b7\u552e\u70ba\u4e8b\u696d\uff0c \u5176\u516c\u53f8\u7814\u767c\u3001\u751f\u7522\u3001\u92b7\u552e\u3002genomic DNA\uff0cPCR\u53cd\u61c9\u7522\u7269\uff0c\u53ef\u4ee5\u5927\u65bc15Kb\uff0c\u5176\u57284\u5ea6C\uff0c\u53ef\u4ee5\u7dad\u6301\u4e0d\u8b8a\u7684\u6548\u80fd\u3002\u5728-20\u5ea6C \u53ef\u4ee5\u7dad\u63011\u5e74\u54c1\u8cea\u90fd\u4e0d\u6703\u6539\u8b8a\u3002DirectPCR Lysis Reagent\u4ee5\u7c97\u8403\u53d6\u7269\u53cd\u61c9\u70ba\u76ee\u7684\uff1b\u82e5\u8981\u7d14\u5316Crude lysates\uff0c\u5176\u65b9\u6cd5\u6bd4\u540c\u4e00\u822cDNA\u7d14\u5316-DNA in crude lysates can be rescued for further analysis. Add NaCl to a final concentration of 250mM , and then add 0.7 volume of isopropanol. DNA will form precipitates. Centrifuge at 4\u00b0C for 2 min, discard supernatant, wash DNA with 1 ml 70% EtOH, and dissolve DNA in 50 \u03bcl 10 mM Tris-HCl (8.0). Use 1 \u03bcl for PCR.<\/p>\n

Brief procedure:<\/strong>
\n1. \u00a0Lyse tails in DirectPCR\u00ae Lysis Reagent.
\n2. \u00a0Incubate for 45 min at 85\u00b0C.
\n3. \u00a0PCR genotyping with 1 \u00b5l lysates.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
DirectPCR DNA Extraction Reagents<\/strong><\/td>\nCat#<\/strong><\/td>\nComments<\/strong><\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (mouse tail)<\/td>\n[101-T]<\/td>\n250 mouse tails (50 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (mouse tail)<\/td>\n[102-T]<\/td>\n500 mouse tails (100 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (yolk sac)<\/td>\n[201-Y]<\/td>\nyolk sacs (50 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (yolk sac)<\/td>\n[202-Y]<\/td>\nYolk sacs (100 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (cell<\/td>\n[301-C]<\/td>\ncultured cells (50 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (cell)<\/td>\n[302-C]<\/td>\ncultured cells (100 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (mouse ear)<\/td>\n[401-E]<\/td>\nmouse ear (25 ml)<\/td>\n<\/tr>\n
DirectPCR Lysis Reagent (mouse ear)<\/td>\n[402-E]<\/td>\nmouse ear (50 ml)<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

HOT!! CRISPR \u55ae\u682aclone\u7d30\u80de | \u6700\u7c21\u55aegDNA\u8403\u53d6\u65b9\u6cd5 \u00a0DirectPCR Lysis R […]<\/p>\n","protected":false},"author":3,"featured_media":6520,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133,88],"tags":[4112],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8050"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8050"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8050\/revisions"}],"predecessor-version":[{"id":32537,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/8050\/revisions\/32537"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6520"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8050"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8050"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8050"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}