{"id":7912,"date":"2016-12-01T14:41:16","date_gmt":"2016-12-01T06:41:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7912"},"modified":"2022-07-25T11:30:30","modified_gmt":"2022-07-25T03:30:30","slug":"plasmidcosmid-dna%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-%e8%b2%a8%e8%99%9frb94-ypd100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=7912","title":{"rendered":"plasmid\/cosmid  \u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865f#RB94-YPD100"},"content":{"rendered":"<p><span style=\"color: #3366ff;\"><strong><a style=\"color: #3366ff;\" href=\"http:\/\/biopioneer.com.tw\/?p=7912\">plasmid DNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865fRB94-YPD100<\/a><\/strong><\/span><\/p>\n<p>T-Pro\u8cea\u9ad4\u8a66\u5291\u76d2\u7cfb\u5217\u5546\u54c1\uff0cT-Pro Mini Prep\u7cfb\u7d71\uff08mini Column\uff09\u7d93\u904e\u512a\u5316\u8655\u7406\uff1b\u5feb\u901f\u5206\u96e2plasmid\u8cea\u9ad4DNA \u6216cosmid DNA\uff1b\u9ad4\u7a4d\u70ba1-5ml\u7d30\u83cc\u57f9\u990a\u6db2\u3002\u5927\u91cf\u88fd\u5099\u9078\u64c7T-Pro Midi \/ Maxi\uff0c\u5176\u7522\u91cf\u70ba500\u03bcg-1mg\u3002\u6b64\u8403\u53d6\u8cea\u9ad4DNA\u7684\u65b9\u6cd5\uff0c\u70ba\u4f7f\u7528\u4fee\u98fe\u7684\u9e7c\u6027\u88c2\u89e3\u6cd5(<strong>alkaline lysis method)<\/strong>\uff0c\u63a5\u8457\u9032\u884cRNase\u8655\u7406\u964d\u4f4egDNA\u6216RNA\u6c61\u67d3\uff0c\u4ee5\u7372\u5f97\u6f84\u6e05\u7684\u7d30\u80de\u88c2\u89e3\u7269\u3002<strong>Silica spin <\/strong>\u6280\u8853\u8207<strong>chaotrophic salt<\/strong><strong>\u4e4b<\/strong>\u7d50\u5408\uff0c\u63d0\u4f9b\u4e86\u53ef\u9760\u7684DNA\u7d50\u5408\u548c<strong>elution<\/strong>\u7cfb\u7d71\u3002\u7d14\u5316\u7684DNA\uff0c\u53ef\u76f4\u63a5\u61c9\u7528\u65bc\u4e0b\u6e38\u5206\u751f\u5be6\u9a57: \u7528\u65bcdigestion\u3001 ligestion\u3001PCR\u53ca\u5b9a\u5e8f\u53cd\u61c9\u3002<\/p>\n<p>T-Pro Plasmid Mini Kit RB94-YPD100 RB94-YPD250 PS1 Buffer 25 ml 60 ml PS2 Buffer 25 ml 60 ml PS3 Buffer 45 ml 90 ml W1 Buffer 45 ml 120 ml W2 Buffer 25 ml 50 ml *** Elution Buffer 15 ml 30 ml RNase A (50 mg\/ml) 50\u03bcl 120\u03bcl 2ml Columns 100 pcs 250 pcs PD columns 100 pcs 250 pcs<\/p>\n","protected":false},"excerpt":{"rendered":"<p>plasmid DNA\u8403\u53d6\u5957\u7d44 | \u8ca8\u865fRB94-YPD100 T-Pro\u8cea\u9ad4\u8a66\u5291\u76d2\u7cfb\u5217\u5546\u54c1\uff0cT-Pro Mi [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":7902,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88,474],"tags":[4154],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7912"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7912"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7912\/revisions"}],"predecessor-version":[{"id":10056,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7912\/revisions\/10056"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/7902"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7912"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7912"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7912"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}