{"id":7607,"date":"2016-11-11T12:04:07","date_gmt":"2016-11-11T04:04:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7607"},"modified":"2024-06-14T10:51:19","modified_gmt":"2024-06-14T02:51:19","slug":"g108-g%e6%96%b0%e5%9e%8b%e5%ae%89%e5%85%a8%e6%a0%b8%e9%85%b8%e6%9f%93%e5%8a%91-safe-green-abmgood%e8%b2%a8%e8%99%9fg108-g","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=7607","title":{"rendered":"G108-G\u65b0\u578b\u5b89\u5168\u6838\u9178\u67d3\u5291 Safe-Green | Abmgood\u8ca8\u865fG108-G"},"content":{"rendered":"

\u00a0\u65b0\u578b\u5b89\u5168\u6838\u9178\u67d3\u5291\u00a0Safe-Green<\/a>\u00a0| <\/a><\/span><\/strong>\u8ca8\u865f<\/strong>G108-G<\/strong><\/a><\/span>\u00a0(<\/strong>Ex490nm\/Em525nm)<\/strong><\/span><\/p>\n

\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

Abmgood<\/strong>\u63d0\u4f9b\u4e00\u7cfb\u5217<\/strong>\u65b0\u578b\u5b89\u5168\u6838\u9178\u67d3\u5291\u00a0SafeView<\/strong>\u7cfb\u5217\u5546\u54c1\uff1b\u5305\u62ec\u4e0d\u540c\u6fc0\u767c\u6ce2\u9577<\/strong>(290-490nm)<\/strong>\uff0c\u53ef\u642d\u914d<\/strong>UV<\/strong>\u89c0\u5bdf\u7bb1\u6216\u85cd\u5149\u89c0\u5bdf\u7bb1\u4f7f\u7528\uff1b\u5176\u4e2d<\/strong>SafeDye\u00a0<\/u><\/strong>(G108-G)<\/u><\/strong>\u53ef\u4ee3\u66ff\u6eb4\u5316\u4e59\u9320(EtBr)\u662floading dye\u4e5f\u662fDNA\u67d3\u5291\uff0c\u7522\u54c1\u7d93\u7531Ames TEST\uff08\u827e\u59c6\u6c0f\u6e2c\u8a66\u6cd5\u3001\u5b89\u59c6\u6c0f\u6e2c\u8a66\u6cd5\u3001\u5b89\u6c0f\u6e2c\u8a66\u6cd5\uff09\u6aa2\u9a57\uff0c\u4e0d\u5177\u57fa\u56e0\u7a81\u8b8a\u53ca\u764c\u5316\u4e4b\u98a8\u96aa (\u5b89\u5168\u6e2c\u8a66\u5831\u544a\u5982\u4e0b\u9801\u6240\u793a) \u3002\u9748\u654f\u5ea60.2\uff0d0.6ng<\/u>\u8207EtBr\u9748\u654f\u5ea60.1ng\u4ee5\u4e0b\u76f8\u7576\u3002\u6b64\u5b89\u5168\u67d3\u5291\u7522\u54c1\u6fc0\u767c(Excitation)\u6ce2\u6bb5\u70ba490nm<\/u>\uff0c\u6563\u5c04\u5149(Emmision)\u70ba525nm<\/u>\u3002\u4f7f\u7528\u65b9\u6cd5<\/strong>\u70ba\u8dd1\u96fb\u6cf3\u6642\u5c07maker\u8207\u6a23\u672c\u4e2d\u52a0\u5165SafeView\u2122 dye (1:5\u500d\u7a00\u91cb)\uff0c\u662f\u4e00\u7a2e\u5feb\u901f\u67d3\u6838\u4e4bloading dye\uff0c\u4e0d\u9700\u5c07\u81a0\u9ad4\u9810\u67d3\u6216\u5916\u67d3\u3002<\/p>\n

G108-G\u00a0\u00a0\u64cd\u4f5c\u624b\u518a<\/span>\u00a0<\/a>\u00a0\u00a0MSDS\u00a0<\/a>\u00a0\u00a0Ligation\u6548\u80fd\u6e2c\u8a66\u5831\u544a<\/a><\/span><\/span><\/p>\n

 <\/p>\n

\"Gel%20Safegreen%201\"
\n<\/p>\n

\n
\n
\n
\n
SafeView\u2122\/Safedye<\/strong>\u7522\u54c1\u61c9\u7528<\/strong><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\u53ef\u4f7f\u7528\u5728\u74ca\u8102\u7cd6(agarose)\u6216\u4e19\u70ef\u91af\u80fa\u51dd\u81a0(polyacrylamide gels)\u4e2d\u7684 dsDNA(double-stranded DNA)\u3001ssDNA(single-stranded DNA)\u53caRNA\u3002\u642d\u914d\u4e0d\u540c\u6ce2\u9577\u6ffe\u7247\u9032\u884cDNA\u5075\u6e2c\u3002In gel staining\u3001Gel extraction\u3001Ligation\u3001Transformation\u3001Transfection<\/p>\n

\"\u67e5\u770b\u4f86\u6e90\u5716\u7247\"<\/p>\n

SafeView\u2122\/Safedye<\/strong>\u7522\u54c1\u6fc0\u767c\u6ce2\u9577\u53ca\u9748\u654f\u5ea6:\u00a0\u5728\u9078\u64c7\u4e0d\u540c\u67d3\u5291\u524d\uff0c\u4e5f\u8acb\u9078\u64c7\u9069\u7684\u6ce2\u9577\u7bc4\u570d\uff0c\u624d\u80fd\u5c07\u5f71\u50cf\u6700\u4f73\u5316\u3002SafeView (G108-G)\u6b64\u5b89\u5168\u67d3\u5291\u7522\u54c1\u6fc0\u767c(Excitation)\u6ce2\u6bb5\u70ba490nm<\/u>\uff0c\u6563\u5c04\u5149(Emmision)\u70ba525nm<\/u>\u3002<\/p>\n

\"\"<\/p>\n

SafeView\u2122\uff0dEtBr\u66ff\u4ee3\u54c1 (\u5167\u67d3\u6216\u5916\u67d3\u4f7f\u7528)\u3002\u4f7f\u7528\u65b9\u6cd5\u8207EtBr\u985e\u4f3c\uff0c\u7121\u9808\u66f4\u6539\u4f7f\u7528\u7fd2\u6163\uff0c\u53ef\u4f7f\u7528\u85cd\u5149\/UV\u5075\u6e2c\u3002SafeView\u2122 Classic\uff0ddsDNA\u8207ssDNA\u53ef\u5448\u73fe\u7da0\u8272\u87a2\u5149\u3001RNA\u5448\u73fe\u7d05\u8272\u87a2\u5149\u3002 SafeView\u2122 FireRed\uff0ddsDNA\u3001ssDNA\u3001\u4ee5\u53caRNA\u5448\u73fe\u7d05\u8272\u87a2\u5149\u3002\u50b3\u7d71EtBr\u67d3\u5291\uff0c\u5176\u9748\u654f\u5ea6\u662f\u6aa2\u6e2c\u81f3\u5c111ng\u4ee5\u4e0b\u7684DNA\uff1b\u65b0\u578b\u5b89\u5168\u67d3\u5291\uff0c\u5177\u6709\u66f4\u9ad8\u7684signal:Bkgrd\u7684\u6bd4\u503c\uff0c\u9748\u654f\u5ea6\u9ad8\u65bc\u50b3\u7d71\u578bEtBr\u67d3\u5291\u3002\u5728\u9078\u64c7\u4e0d\u540c\u67d3\u5291\u524d\uff0c\u4e5f\u8acb\u9078\u64c7\u9069\u7684\u6ce2\u9577\u7bc4\u570d\uff0c\u624d\u80fd\u5c07\u5f71\u50cf\u6700\u4f73\u5316\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u6ce2\u9577\uff08Ex\/Em\uff09<\/strong><\/td>\n\u9748\u654f\u5ea6\uff08ng\uff09<\/strong><\/td>\n\u5305\u88dd<\/strong><\/td>\n<\/tr>\n
SafeView\u2122 Classic<\/strong>\uff0d\u5167\u67d3<\/strong><\/td>\n<\/tr>\n
G108<\/td>\nSafeView\u2122 Classic<\/td>\n\n

490nm\/ 635nm<\/p>\n

490nm\/520nm<\/td>\n

0.1\uff0d0.3<\/td>\n1ml<\/td>\n<\/tr>\n
SafeView Plus\u2122<\/strong>\uff0d\u5916\u67d3<\/strong><\/td>\n<\/tr>\n
G468<\/td>\nSafeView Plus\u2122<\/td>\n290nm\/520nm<\/td>\n0.05\uff0d0.1<\/td>\n1ml<\/td>\n<\/tr>\n
SafeView\u2122 dyes<\/strong><\/td>\n<\/tr>\n
G108-G<\/td>\nSafe-Green\u2122<\/td>\n490nm\/525nm<\/td>\n0.2\uff0d0.6<\/td>\n1ml<\/td>\n<\/tr>\n
G108-R<\/td>\nSafe-Red\u2122<\/td>\n490nm\/630nm<\/td>\n0.3\uff0d0.8<\/td>\n1ml<\/td>\n<\/tr>\n
G108-W<\/td>\nSafe-White\u2122<\/td>\n370nm\/470nm<\/td>\n0.2\uff0d0.5<\/td>\n1ml<\/td>\n<\/tr>\n
G108-P<\/td>\nSafe-Pack\u2122<\/td>\n\u53c3\u7167G108-G\u3001R\u3001W<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/span>\u00a0\u00a0\u00a0\u5fae\u751f\u7269\u57f9\u990a\u57fa<\/a>\u00a0|\u00a0\u5fae\u751f\u7269\u6297\u751f\u7d20<\/a>\u00a0|\u00a0\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44<\/a>\u00a0|\u00a0CMOS\u87a2\u5149\u76f8\u6a5f<\/a>\u00a0|Mupid 2 DNA\u6c34\u5e73\u96fb\u6cf3\u69fd<\/a><\/span><\/strong><\/p>\n

SafeView\u2122\/Safedye<\/strong>\u4f7f\u7528\u65b9\u6cd5:<\/strong><\/p>\n

\"\u00e4\u00bd\u00bf\u00e7\u0094\u00a8\u00e6\u0096\u00b9\u00e6\u00b3\u0095A\"(\u5167\u67d3) SafeView\u2122 Classic\u3001FireRed\u4f7f\u7528\u65b9\u6cd5<\/strong>100ml agarose\u6eb6\u6db2\u4e2d\u52a0\u51655ul SafeView\u2122 Classic \u5f8c\u88fd\u81a0\u3002SafeDye (\u662floading dye\u4e5f\u662fDNA\u67d3\u5291)\u7cfb\u5217\u7522\u54c1\u5305\u62ec\uff1aSafe-Green\u3001Red\u3001White\u3001Pack (loading dye\u67d3\u5291)\uff0c\u4f7f\u7528\u65b9\u6cd5<\/strong>\u70ba\u8dd1\u96fb\u6cf3\u6642\u5c07maker\u8207\u6a23\u672c\u4e2d\u52a0\u5165SafeView\u2122 dye (1:5\u500d\u7a00\u91cb)\uff0c\u662f\u4e00\u7a2e\u5feb\u901f\u67d3\u6838\u4e4bloading dye\uff0c\u4e0d\u9700\u5c07\u81a0\u9ad4\u9810\u67d3\u6216\u5916\u67d3\u3002(\u5916\u67d3) SafeView Plus\u2122\uff0d\u4f5c\u70ba\u5916\u67d3<\/strong>\u4f7f\u7528\u3002\u4e0d\u9700\u9000\u67d3\u6b65\u9a5f\u3002\u9748\u654f\u5ea6\u66f4\u4f73\u3002SafeView Plus\u2122\uff1aDNA\u96fb\u6cf3\u5b8c\u6210\u5f8c\uff0c\u5c07\u81a0\u9ad4\u6ce1\u5165\u542b\u6709SafeView Plus\u2122\u7684\u96fb\u6cf3\u7de9\u885d\u6db2\uff081:10,000\u7a00\u91cb\uff09\uff0c\u5ba4\u6eab\u4e0b\u53cd\u61c910-40\u5206\u9418\u5373\u53ef\u9032\u884c\u5075\u6e2c\u3002<\/p>\n

\"\"<\/p>\n

SafeView\u2122\/Safedye<\/strong>\u4f7f\u7528\u9808\u77e5<\/strong><\/p>\n

\u4e00\u822c\u6838\u9178\u89c0\u5bdf\u7bb1\u5206\u70baUV\u8207\u85cd\u5149\u4e8c\u7a2e\u7cfb\u7d71\uff1b<\/strong>\u50b3\u7d71\u7684UV Box\u89c0\u5bdf\u7bb1, \u5177\u6709\u9577\u6ce2(365nm)\u3001\u4e2d\u6ce2(312nm)\u3001\u77ed\u6ce2(256nm)\u6ce2\u6bb5\u6fc0\u767c\u3002\u9577\u6ce2\u80fd\u91cf\u8f03\u5f31 (365nm) \uff0c\u9069\u5408\u5207\u81a0\u7b49\u81a0\u9ad4\u56de\u6536(gel extraction)\u7528\u3002\u77ed\u6ce2\u80fd\u91cf\u5f37(256nm)\uff0c\u53ef\u4ee5\u53d6\u5f97\u8f03\u660e\u986f\u7684\u5f71\u50cf\u3002\u65b0\u4e00\u578bSafe Blue\u85cd\u5149\u89c0\u5bdf\u7bb1\uff0c\u53d6\u4ee3UV\u7684\u6ce2\u9577\u70ba470nm \u85cd\u5149\u6ce2\u6bb5\uff0c\u6e1b\u5c11UV\u5149\u7dda\u5c0d\u6a23\u54c1\u53ca\u773c\u775b\u9020\u6210\u7684\u50b7\u5bb3\u3002\u642d\u914d\u4e0d\u540c\u7684\u6838\u9178\u89c0\u5bdf\u7bb1\uff0cabmgood\u63d0\u4f9b\u4e0d\u540c\u6ce2\u9577\u4e4b\u6838\u9178\u67d3\u5291\uff0c\u5c07\u6838\u9178\u5f71\u50cf\u6700\u4f73\u5316\u3002\u4e5f\u6e1b\u5c11\u505a\u5be6\u9a57\u8a98\u764c\u4e4b\u98a8\u96aa\u767c\u751f\u3002\u50b3\u7d71\u7684\u6838\u9178\u67d3\u5291, EtBr (Biovisioin #1203-10)<\/strong>\u6eb4\u5316\u4e59\u9320 (\u7e2e\u5beb\u70baEtBr)\uff0c\u662f\u4e00\u7a2e\u6838\u9178\u67d3\u5291\uff0c\u7528\u70ba\u74ca\u8102\u7cd6\u51dd\u81a0\u96fb\u6cf3\u5f8c\uff0c\u6838\u9178\u4e4b\u67d3\u8272\u3002\u5728UV\u7d2b\u5916\u5149\u7167\u5c04\u4e0b\uff0c\u672a\u7d50\u5408\u7684ErBr\u53ef\u88ab\u6fc0\u767c\u51fa\u6a59\u7d05\u8272\u87a2\u5149\uff1b\u7d50\u5408\u7684DNA\u6216\u96d9\u80a1RNA\uff0c\u87a2\u5149\u5f37\u5ea6\u6703\u589e\u5f3720\u500d\uff0c\u4f7f\u5f97\u6838\u9178\u96fb\u6cf3\u5f8c\u53ef\u5206\u8fa8\u6838\u9178\u76f8\u5c0d\u7684\u5206\u5b50\u91cf\u3002EtBr\u5176\u6fc0\u767c(Excitation)\u6ce2\u6bb5\u70ba310nm\uff0c\u6563\u5c04\u5149(emmision)\u70ba570nm\u3002\u9700\u6ce8\u610f\u6eb4\u5316\u4e59\u9320 (EtBr)\u70ba\u662f\u4e00\u7a2e\u5f37\u7684\u8a98\u8b8a\u5291intercalator\uff0c\u53ef\u81f4\u764c\u6216\u81f4\u7578\u3002\u64cd\u4f5c\u6642\u5fc5\u9808\u4f69\u6234\u624b\u5957\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/th>\nSafe-Green\u00a0 is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.<\/td>\n<\/tr>\n
SKU<\/th>\nG108-G<\/td>\n<\/tr>\n
SafeView Series<\/th>\nLoading Dye<\/td>\n<\/tr>\n
LED Viewer Compatibility<\/th>\nYes<\/td>\n<\/tr>\n
Stain Color<\/th>\nGreen<\/td>\n<\/tr>\n
Applications<\/th>\nSafe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.<\/td>\n<\/tr>\n
Note<\/th>\n\n

All SafeView DNA Stains are ISO-13485 certified.<\/p>\n

Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).<\/td>\n<\/tr>\n

Shipping Conditions<\/th>\nShipped on blue ice packs.<\/td>\n<\/tr>\n
Storage Condition<\/th>\nStore at 4\u00b0C for up to 2 years.<\/td>\n<\/tr>\n
Unit quantity<\/th>\n1.0 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5b89\u5168\u6e2c\u8a66\u5831\u544a (Ligation Efficiency Test Report<\/a>)<\/strong><\/span><\/p>\n

\u6838\u9178\u67d3\u5291\u6e2c\u8a66\u5831\u544a\uff0c\u70ba\u6bd4\u8f03\u5b89\u5168\u67d3\u5291Safe-GreenTM<\/sup>\u8207\u6eb4\u5316\u4e59\u9320(\u00a0Ethidium Bromide)\u4f7f\u7528\u76f8\u6bd4\uff0cSafe-GreenTM<\/sup>\u5c0d\u9023\u63a5\u53cd\u61c9(\u00a0ligation reactions)\u662f\u5426\u6709\u8ca0\u9762\u5f71\u97ff<\/p>\n

To investigate if Safe-GreenTM has any negative effect on ligation reactions compared to using Ethidium Bromide.<\/p>\n

\u6548\u80fd\u6e2c\u8a66\u64cd\u4f5c\u7a0b\u5e8f<\/strong><\/p>\n

1\uff09\u4f7f\u7528XhoI\u7dda\u6027\u5316(Linearize ) 6 \u00b5g\u8f09\u9ad4(vector)\u3002<\/p>\n

2\uff09\u5c073 \u00b5g\u7dda\u6027\u5316\u8f09\u9ad4 (linearized vector)\/ DNA Loading dye \/\u6eb4\u5316\u4e59\u9320(Ethidum Bromide )\u51dd\u81a0\u4e0a\u904b\u884c; \u53e6\u5916\u5728\u6c92\u6709\u6eb4\u5316\u4e59\u9320\u53d63 \u00b5g\u7dda\u6027\u5316\u7684\u8f09\u9ad4+ SafeGreen (\u542bLoading dye) )\u51dd\u81a0\u4e0a\u904b\u884c\u3002<\/p>\n

3\uff09\u4f7f\u7528Column-Pure DNA\u51dd\u81a0\u63d0\u53d6\u8a66\u5291\u76d2\uff0c\u4ee525 \u00b5l\u6d17\u812b\u7de9\u885d\u6db2(elution buffer)\u6d17\u812bDNA\u3002<\/p>\n

4\uff09\u4f7f\u75284 \u00b5l\u7d14\u5316\u7684\u7dda\u6027\u5316\u8f09\u9ad4\u548cT4 DNA\u9023\u63a5\u9176\u5efa\u7acb\u9023\u63a5\u53cd\u61c9, \u8a2d\u7f6eno ligase\u63a7\u4ef6\u4ee5\u8a55\u4f30\u672a\u5207\u5272\u7684\u8f09\u9ad4\u80cc\u666f\u7684\u6c34\u5e73\uff0c\u5982\u4e0b\u6240\u793a\uff1a<\/p>\n

5 ) \u8f49\u5316(Transform)\u81f3LB +\u6c28\u8290\u9752\u9ef4\u7d20\u5e73\u677f(ampicillin plates)\u4e0a\u4ee5\u9078\u64c7\u542b\u6709\u6240\u9700\u7684\u91cd\u7d44\u8cea\u7c92\u3002\u7b2c\u4e8c\u5929\u6bd4\u8f03\u83cc\u843d\u8a08\u6578\u3002<\/p>\n

\u5b89\u5168\u67d3\u5291(Safe-Green)\u8207\u50b3\u7d71\u6eb4\u5316\u4e59\u9320\u76f8\u6bd4\uff0c\u4f7f\u7528Safe-Green\u6642\uff0c\u9023\u63a5\u6548\u7387\u6c92\u6709\u5dee\u7570<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

Dye intensity was highest in original gel, but intensity did not observe a gradual decrease as the number of re-boiling increased. However, structural integrity of the agarose gel was noticeably weaker by the 5th re-boiling, which led to a rip of the wells (well 2-6) during loading, hence, the observed smear.
\nConclusion:
\nRe-boiling of up to 5 times did not significantly impact the performance of the SafeView dye (G108). However, integrity of the agarose gel may weaken over time.<\/p>\n","protected":false},"excerpt":{"rendered":"

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