{"id":7210,"date":"2016-10-21T14:51:35","date_gmt":"2016-10-21T06:51:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7210"},"modified":"2023-08-25T13:48:26","modified_gmt":"2023-08-25T05:48:26","slug":"%e5%bf%ab%e9%80%9f%e5%85%a7%e6%af%92%e7%b4%a0%e5%8e%bb%e9%99%a4%e5%95%86%e5%93%81%e5%a5%97%e7%b5%84-toxout-rapid-endotoxin-removal-kit%e8%b2%a8%e8%99%9fk2501-5","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=7210","title":{"rendered":"\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u5546\u54c1\u5957\u7d44 |ToxOut\u2122 Rapid Endotoxin Removal Kit\u8ca8\u865fK2501-5 (abcam, ab239707)"},"content":{"rendered":"

5<\/span><\/strong>\u5206\u9418\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u5546\u54c1\u5957\u7d44<\/strong>\u00a0| \u8ca8\u865fK2501-5\u00a0 \u00a0(abcam, ab239707)\u00a0 5\u500b\u53cd\u61c9 (\u6bcf\u500bcolumn\u53ef\u518d\u91cd\u8986\u4f7f\u75285\u6b21)<\/span><\/a><\/span><\/p>\n

ToxOut\u2122 Rapid Endotoxin Removal Kit<\/span><\/p>\n

polymyxin B\u53bb\u9664\u5167\u6bd2\u7d20\u4e4b\u539f\u7406:<\/strong><\/span><\/p>\n

Polymyxin B<\/span>\uff08PMB<\/span>\uff09<\/span>\u662f\u5f9e\u591a\u7c98\u82bd\u5b62\u687f\u83cc\u4e2d\u5206\u96e2\u51fa\u4f86\u7684\u4e00\u7a2e\u5f37\u967d\u96e2\u5b50\u74b0\u6297\u751f\u7d20\uff0c\u662f\u76ee\u524d\u767c\u73fe\u7684\u552f\u4e00<\/span><\/strong>\u4e00\u7a2e\u80fd\u7279\u7570\u6027\u89aa\u548c\u5438\u9644\u5167\u6bd2\u7d20(<\/span>\u8102\u591a\u7cd6)<\/span>\u7684\u6297\u751f\u7d20\u3002\u5c07Polymyxin B<\/span>\u901a\u904e\u5171\u50f9\u5076\u806f\u7684\u65b9\u5f0f\u56fa\u5b9a\u5728\u74ca\u8102\u7cd6\u73e0\u4e0a\uff0c\u5229\u7528Polymyxin B<\/span>\u80fd\u5920\u7279\u7570\u6027\u89aa\u548c\u5167\u6bd2\u7d20\u7684\u7279\u6027\uff0c\u9664\u53bb\u6eb6\u6db2\u548c\u751f\u7269\u88fd\u54c1\u4e2d\u7684\u5167\u6bd2\u7d20\u3002Biovision\u63d0\u4f9b\u4e00\u7cfb\u5217\u5167\u6bd2\u7d20\u8a66\u5291\u5546\u54c1\uff1b\u5176\u4e2dToxOutTM<\/sup>\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u8a66\u5291\u76d2 (\u8ca8\u865fK2501) \u4e2d\uff0c\u6709\u6548\u6d88\u9664\u6eb6\u6db2\u4e2d\u7684\u5167\u6bd2\u7d20<0.05EU \/ ml\uff0c\u9ad8\u7d50\u5408\u5bb9\u91cf(Binding Capacity)\uff1a\u9ad8\u9054up to\u00a0 9 x 108<\/sup> EU\/ml resin\u3002\u9ad8\u6548\u6a23\u54c1\u56de\u6536\u7387(Sample Recovery) \u86cb\u767d\u8cea\u6eb6\u6db2\u6a23\u54c1\u56de\u6536\u301c90\uff05\u3002\u89aa\u548c\u6027\u57fa\u8ceapolymyxin B\uff0c50\uff05\u6f3f\u6db2\u6eb6\u65bc20\uff05\u4e59\u9187\u4e2d\u3002Columns Content: 5 x 100 \u00b5l ToxOutTM<\/sup> Rapid Endotoxin Removal Agarose (Cat. No. 7941-5) \u61c9\u7528\u65bc\u86cb\u767d\u8cea\u5167\u6bd2\u7d20\u4e4b\u79fb\u9664\u6216\u85e5\u7406\u5316\u5408\u7269\u5167\u6bd2\u7d20\u4e4b\u79fb\u9664\u3002<\/p>\n

\"\"<\/p>\n

\u5167\u6bd2\u7d20\u53bb\u9664\u8a66\u5291\u76d2\uff08\u5feb\u901f\uff09(ab239707) \u5f9e\u86cb\u767d\u8cea\u6eb6\u6db2\u6a23\u54c1\u4e2d\u53bb\u9664 >90% \u7684\u5167\u6bd2\u7d20\u3002\u5167\u6bd2\u7d20\u5bb9\u91cf\u548c\u5167\u6bd2\u7d20\u6548\u7387\u901a\u904e\u5728 200 \u00b5L BSA (10 mg\/mL) \u4e2d\u52a0\u5165 1 x 10 8<\/sup> EU \/mL LPS \u7684\u5177\u6709\u6311\u6230\u6027\u7684 0.1 mL \u6a39\u8102\u4f86\u78ba\u5b9a\u3002\u901a\u904e\u5c07\u6a23\u54c1\u91cd\u65b0\u52a0\u8f09\u5230\u53cd\u590d\u518d\u751f\u7684\u8272\u8b5c\u67f1\u4e2d\uff0cBSA \u6eb6\u6db2\u4e2d\u7684\u5167\u6bd2\u7d20\u52a0\u6a19\u964d\u81f3 <0.05 EU\/mL\u3002\u7b2c\u4e00\u500b\u6392\u6bd2\u9031\u671f\u7684\u6700\u9ad8\u5167\u6bd2\u7d20\u5bb9\u91cf\u70ba 9.99 x 10 8<\/sup> EU\/mL\u30025 \u500b\u6392\u6bd2\u9031\u671f\u7684\u5e73\u5747\u6548\u7387\u70ba 94.5%\uff0c5 \u500b\u6392\u6bd2\u9031\u671f\u7684\u5e73\u5747\u86cb\u767d\u8cea\u56de\u6536\u7387\u70ba 89.9%\u3002<\/p>\n

\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664 (\u5373\u7528\u578b\u96e2\u5fc3\u67f1)\u00a0<\/span><\/strong><\/p>\n

\u5305\u542b 100uL \u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u74ca\u8102\u7cd6\u3002\u4efb\u4f55\u6642\u5019\u90fd\u4e0d\u8981\u8b93\u6a39\u8102\u8b8a\u4e7e\u3002\u4f7f\u7528\u5f8c\u5132\u5b58\u5728\u542b\u6709 20% \u4e59\u9187\u7684\u5167\u6bd2\u7d20\u5e73\u8861\u7de9\u885d\u6db2\u4e2d\u3002\u5982\u679c\u8cfc\u8cb7\u55ae\u7368\u7684\u7de9\u885d\u6db2\uff08\u5167\u6bd2\u7d20\u53bb\u9664\u5e73\u8861\u7de9\u885d\u6db2 (ab278802)\u3001\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u518d\u751f\u7de9\u885d\u6db2 (ab269281)\u3001\u5feb\u901f\u53bb\u9664\u6d17\u6ecc\u7de9\u885d\u6db2 (ab278801)\uff09\uff0c\u6bcf\u6839\u7ba1\u67f1\u6700\u591a\u53ef\u91cd\u8907\u4f7f\u7528 5 \u6b21\u3002<\/p>\n

\u5167\u6bd2\u7d20\u4ecb\u7d39<\/strong>:<\/strong><\/span><\/p>\n

\u5167\u6bd2\u7d20(Endotoxins )\u662f\u4f4d\u65bc\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc(gram negative bacteria)\u5916\u819c(outer membrane )\u4e2d\u7684\u8102\u591a\u91a3\uff08LPS\uff09\u8907\u5408\u7269\u3002\u55ae\u500b\u5927\u8178\u687f\u83cc\u542b\u6709\u5927\u7d042\u767e\u842cLPS\u4e4b\u5206\u5b50\uff082-20fg \/\u7d30\u80de\uff09\u3002\u5728\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u5927\u91cf\u7684\u5167\u6bd2\u7d20\u88ab\u812b\u843d\u4e26\u4e14\u53ef\u4ee5\u5bb9\u6613\u5730\u6c61\u67d3\u5be6\u9a57\u5ba4\u5668\u76bf\uff0c\u7de9\u885d\u6db2\u53ca\u4e0b\u6e38\u7522\u54c1\u3002In vitro\uff0c\u5167\u6bd2\u7d20\u5728\u57fa\u65bc\u7d30\u80de\u7684\u7814\u7a76\u4e2d\u5f15\u8d77\u5404\u7a2e\u554f\u984c\uff1bIn vivo\uff0c\u5167\u6bd2\u7d20\u53ef\u5f15\u8d77\u5404\u7a2e\u526f\u4f5c\u7528\uff0c\u5305\u62ec\u5bbf\u4e3b\u751f\u7269\u9ad4\u4e2d\u7684\u708e\u75c7\u53cd\u61c9(inflammatory response)\uff0c\u5668\u5b98\u8870\u7aed(organ failure)\u6216\u81bf\u6bd2\u6027\u4f11\u514b(septic shock)\u3002\u5167\u6bd2\u7d20\u5c0d\u7d30\u80de\u7684\u57f9\u990a\u5177\u6709\u6709\u5bb3\u7684\u5f71\u97ff\uff0c\u4e26\u4e14\u53ef\u5c0e\u81f4\u4e0d\u53ef\u518d\u73fe\u7684\u6216\u9670\u6027\u5be6\u9a57\u7684\u7522\u751f\u6578\u64da\u3002<\/p>\n

\u7c21\u55ae\u64cd\u4f5c\u7a0b\u5e8f:<\/strong><\/span><\/p>\n

The kit quickly and effectively eliminates endotoxins to < 0.05 EU\/ml and is ideal for processing small-scale solution sample (0.1-0.5 mL)<\/p>\n

\u5c07\u7ba1\u67f1\u9032\u884c\u4ee5\u4e0b\u53cd\u61c9 (\u52a0\u5165-\u96e2\u5fc3)<\/p>\n

—-\u79fb\u53bb\u7ba1\u67f1\u4e2d\u4e0b\u9762\u7684\u6813\uff0c\u4e26\u653e\u81f3ToxOut\u2122Endotoxin-free Collection Tube. Spin\u96e2\u6389 storage solution.<\/p>\n

—-\u52a0\u5165Equilibration(total two times)\u6eb6\u6db2\u4e26\u96e2\u5fc3-> \u52a0\u5165Regenerate\u6eb6\u6db2\u4e26\u96e2\u5fc3-> Wash(total 3 times)-> \u52a0\u5165Equilibration (total two times) \u6eb6\u6db2\u4e26\u96e2\u5fc3-><\/p>\n

—- Place column in a new ToxOutTM Endotoxin-free Collection tube<\/p>\n

—- \u52a0\u5165sample (0.1 – 0.5 ml)\u91cd\u529b\u6d41\u52d5(3-5 times), \u5c07cap \u53ca plug\u88dd\u56de\u7ba1\u67f1<\/p>\n

—- room temperature or 4 \u030aC for 1 hour<\/p>\n

—-\u96e2\u5fc3\u6536\u96c6\u53bb\u9664\u5b8c\u5167\u6bd2\u7d20\u4e4bSample<\/p>\n

Rapid Endotoxin Removal Spin Column Ready to use as supplied.<\/p>\n

Contains 100uL of Rapid Endotoxin Removal Agarose. Do not let the resin dry out at any time. Store in Endotoxin Equilibration Buffer containing 20% ethanol after use. Each column can be re-used up to 5 times, providing the separate buffers are purchased (Endotoxin Removal Equilibration Buffer (ab278802), Rapid Endotoxin Removal Regeneration Buffer (ab269281), Rapid Removal Wash Buffer (ab278801)).<\/p>\n

\u5b8c\u6574\u64cd\u4f5c\u624b\u518a\u8acb\u6309\u6b64<\/a><\/span><\/p>\n

\u91cd\u67f1\u82e5\u91cd\u8986\u4f7f\u7528Pace plug and cap back and store the column in 200 \u00b5l ToxOutTM<\/sup> Endotoxin Removal Equilibration buffer with 20% ethanol at 4 \u030aC<\/p>\n\n\n\n\n\n\n\n
Cat # +Size<\/th>\nK2501-5\u00a0\u00a0(abcam, ab239707)<\/td>\n<\/tr>\n
Size<\/th>\n5 columns, up to\u00a0 9 x 108<\/sup>\u00a0EU\/ml resin<\/td>\n<\/tr>\n
Highlights<\/th>\n\u2022 High Binding Capacity: up to 9 x 108<\/sup> EU\/ml resin
\n\u2022 High Sample Recovery: ~90% recovery with protein solution samples
\n\u2022 Column Content: 5 x 100 \u00b5l ToxOutTM Rapid Endotoxin Removal Agarose (Cat. No. 7941-5)
\n\u2022 Resin Content: affinity matrix of polymyxin B, supplied as 50% slurry in 20% ethanol
\n\u2022 Each column can be reused up to 5 times
\n\u2022 Rapid removal: only require 5 minutes for each removal cycle<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n4\u00b0C Do not freeze<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog#<\/a><\/td>\n\u00a0Product Name<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a07943-50<\/td>\n\u00a0ToxOut\u2122 Endotoxin Free PBS\u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n
\u00a07937-50<\/td>\n\u00a0ToxOut\u2122 Endotoxin Free Collection Tube<\/a><\/strong><\/td>\n\u00a050 tubes<\/td>\n<\/tr>\n
\u00a07944-2<\/td>\n\u00a0ToxOut\u2122 Endotoxin Free Glass Vials<\/a><\/strong><\/td>\n\u00a02 ml (10 vials)<\/td>\n<\/tr>\n
\u00a07944-10<\/td>\n\u00a0ToxOut\u2122 Endotoxin Free Glass Vials<\/a><\/strong><\/td>\n\u00a010 ml (10 vials)<\/td>\n<\/tr>\n
\u00a07938-50<\/td>\n\u00a0ToxOut\u2122 Endotoxin Free Water\u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n
\u00a07941-5<\/td>\n\u00a0ToxOut\u2122 Endotoxin Rapid Removal Agarose<\/a><\/strong><\/td>\n\u00a05 ml<\/td>\n<\/tr>\n
\u00a07940-50<\/td>\n\u00a0ToxOut\u2122 Endotoxin Removal Equilibration Buffer\u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n
\u00a07942-5<\/td>\n\u00a0ToxOut\u2122 High Capacity Endotoxin Removal Agarose \u00a0<\/a><\/strong><\/td>\n\u00a05 ml<\/td>\n<\/tr>\n
\u00a0K2502-5<\/td>\n\u00a0ToxOut\u2122 High Capacity Endotoxin Removal Kit\u00a0<\/a><\/strong><\/td>\n\u00a05 kit<\/td>\n<\/tr>\n
\u00a07945-50<\/td>\n\u00a0ToxOut\u2122 High Capacity Endotoxin Removal Regeneration Buffer \u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n
\u00a07946-50<\/td>\n\u00a0ToxOut\u2122 High Capacity Endotoxin Removal Wash Buffer\u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n
\u00a0K2501-5<\/td>\n\u00a0ToxOut\u2122 Rapid Endotoxin Removal Kit<\/a><\/strong><\/td>\n\u00a05 kits<\/td>\n<\/tr>\n
\u00a07939-50<\/td>\n\u00a0ToxOut\u2122 Rapid Endotoxin Removal Regeneration Buffer\u00a0<\/a><\/strong><\/td>\n\u00a050 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

5\u5206\u9418\u5feb\u901f\u5167\u6bd2\u7d20\u53bb\u9664\u5546\u54c1\u5957\u7d44\u00a0| \u8ca8\u865fK2501-5\u00a0 \u00a0(abcam, ab239707)\u00a0 5\u500b\u53cd\u61c9 (\u6bcf […]<\/p>\n","protected":false},"author":3,"featured_media":6512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5380,508,134,179],"tags":[4328],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7210"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7210"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7210\/revisions"}],"predecessor-version":[{"id":45374,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7210\/revisions\/45374"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7210"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7210"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7210"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}