{"id":7162,"date":"2016-10-18T15:35:55","date_gmt":"2016-10-18T07:35:55","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7162"},"modified":"2022-07-25T15:22:26","modified_gmt":"2022-07-25T07:22:26","slug":"8-ohdg-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk4160-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=7162","title":{"rendered":"8-OHdG ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fK4160-100"},"content":{"rendered":"

8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA<\/strong>\u8a66\u5291\u76d2<\/strong> | \u8ca8\u865fK4160-100<\/a><\/span><\/p>\n

8OHdG; 7,8-Dihydro-8-Oxo-2′-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2′-Deoxyguanosine; 8-Oxo-DG<\/p>\n

\u7522\u54c1\u898f\u683c<\/strong>:\u00a0<\/strong>8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a<\/span><\/a>\u8a66\u5291\u76d2<\/span> | \u63d0\u4f9b\u7c21\u6613\u65b9\u4fbf\u7701\u6642\u4e4b\u65b9\u6cd5\uff0c\u5feb\u901f\u6e2c\u91cf\u6a23\u54c1\u4e2d8-OHdG \u4e4b\u542b\u91cf\u3002\u6e2c\u91cf\u7bc4\u570d\u70ba1.563 – 100 ng\/ml\u3002\u6700\u4f4e\u9748\u654f\u5ea6Sensitivity: < 0.94 ng\/ml\u3002BioVision\u63d0\u4f9b\u4e00\u7cfb\u5217\u6e96\u78ba\u3001\u5373\u7528\u578b\u4e4bELISA\u8a66\u5291\u76d2\uff1b\u7528\u65bc\u6aa2\u6e2c\u548c\u91cf\u5316\u86cb\u767d\u8cea\u6216\u5c0f\u5206\u5b50\u7684\u5b58\u5728\u3002\u4ee5ELISA\u70ba\u57fa\u790e\u4e4b\u5075\u6e2c\u65b9\u6cd5\u512a\u9ede\u70ba\u6e96\u78ba-\u5177\u6709\u8f03\u4f4e\u7684\u5167\u548c\u6e2c\u5b9a\u9593\u8b8a\u7570\u6027\u7684\u53ef\u91cd\u8907\u7d50\u679c\u3001\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\u3001\u4f4e\u80cc\u666f\uff1b\u901a\u7528\u6027-\u5ee3\u6cdb\u7684\u6a23\u54c1\u985e\u578b\uff0c\u5982\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d44\u7e54\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\u3001\u5c3f\u6db2\u548c\u8a31\u591a\u5176\u4ed6\u751f\u7269\u6db2\u9ad4\u3002\u65b9\u4fbf\u6027-\u7121\u9700\u7279\u6b8a\u8655\u7406\u6216\u8655\u7f6e\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: \u00a0<\/strong>8-\u7fa5\u57fa-2-\u812b\u6c27\u9ce5\u82f7\uff088-hydroxy-2-deoxyguanosine , 8-OHdG\uff09\u662f\u812b\u6c27\u9ce5\u82f7(deoxyguanosine)\u7684\u6c27\u5316\u884d\u751f\u7269 (oxidized derivative )\u3002 \u7d30\u80de\u51678-ox-dG (8-hydroxy-2′-deoxyguanosine) \u7684\u6fc3\u5ea6\u662foxidative stress\u7684\u6e2c\u91cf\u6307\u6a19\u3002 \u5b83\u88ab\u8a8d\u70ba\u662foxidative stress\u548ccarcinogenesis\u7684\u95dc\u9375\u6027\u751f\u7269\u6a19\u8a8c\u7269\u3002 \u8a72ELISA\u8a66\u5291\u76d2\u4f7f\u7528\u7af6\u722dELISA(Competitive-ELISA)\u65b9\u6cd5\u3002\u8a72\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684microtiter plate\u70ba8-OHdG\u9810\u5305\u88ab\u3002\u5728\u53cd\u61c9\u671f\u9593\uff0c\u6a23\u54c1\u6216\u6a19\u6e96\u54c1\u4e2d\u76848-OHdG\u8207\u56fa\u5b9a\u91cf\u76848-OHdG\u5728\u56fa\u76f8\u652f\u6301\u7269\u4e0a\uff0c\u7af6\u722d8-OHdG\u7279\u7570\u6027\u7684\u751f\u7269\u7d20\u5316\u6aa2\u6e2c\u6297\u9ad4\u4e4b\u4f4d\u9ede\u3002 \u6a23\u54c1\u4e2d8-OHdG\u7684\u6fc3\u5ea6\uff0c\u901a\u904e\u6bd4\u8f03\u6a23\u54c1\u4e2d\u7684O.D.\u7684\u6a23\u54c1\u5230\u6a19\u6e96\u66f2\u7dda\u30028-hydroxy-2′-deoxyguanosine (8-OHdG) is an oxidized derivative of deoxyguanosine. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress. It is considered as a critical biomarker of oxidative stress and carcinogenesis. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. During the reaction,8-OHdG in the sample or standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 8-OHdG. The concentration of 8-OHdG in the samples is determined by comparing the O.D. of the samples to the standard curve.<\/p>\n

8-<\/strong>\u7fa5\u57fa<\/strong>-2-<\/strong>\u812b\u6c27\u9ce5\u82f7<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n

Microplate: 96 wells
\nLyophilized Standard
\nSample \/ Standard dilution buffer
\nBiotin- detection antibody (Concentrated)
\nAntibody dilution buffer
\nHRP-Streptavidin Conjugate (SABC)
\nSABC dilution buffer
\nTMB substrate
\nStop Solution
\nWash buffer (25X)
\nPlate sealers<\/p>\n

\u66f4\u591aELISA\u5206\u6790\u5957\u7d44<\/strong>\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0Catalog #<\/a><\/td>\n\u00a0Product Name+<\/a><\/td>\n\u00a0Size<\/a><\/td>\n<\/tr>\n
\u00a0K4160-100<\/td>\n\u00a08-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA Kit\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4188-100<\/td>\n\u00a0ABCG2 (Human) ELISA Kit\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4769-100<\/td>\n\u00a0Activin A (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4901-100<\/td>\n\u00a0Adiponectin (human) Elisa Assay Kit<\/a><\/strong><\/td>\n\u00a096 assays<\/td>\n<\/tr>\n
\u00a0K4902-100<\/td>\n\u00a0Adiponectin (mouse) Elisa Assay Kit<\/a><\/strong><\/td>\n\u00a096 assays<\/td>\n<\/tr>\n
\u00a0K4903-100<\/td>\n\u00a0Adiponectin\/gACRP30 (rat) Elisa Assay Kit<\/a><\/strong><\/td>\n\u00a096 assays<\/td>\n<\/tr>\n
\u00a0K4768-100<\/td>\n\u00a0Adipsin\/Factor D (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4003-100<\/td>\n\u00a0Adrenocorticotropic Hormone (ACTH) (human) ELISA Kit\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4166-100<\/td>\n\u00a0AKT1\/PKB (Human) ELISA Kit\u00a0<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4802-100<\/td>\n\u00a0Angiogenin (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K7115-100<\/td>\n\u00a0Angiopoietin-1 (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K7116-100<\/td>\n\u00a0Angiopoietin-2 (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4755-100<\/td>\n\u00a0Angiostatin (human) ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n
\u00a0K4914-100<\/td>\n\u00a0ANGPTL3 (human) Serum ELISA Kit<\/a><\/strong><\/td>\n\u00a0100 assays<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

8-hydroxy-2′-deoxyguanosine (8-OHdG) ELISA\u8a66\u5291\u76d2 | \u8ca8 […]<\/p>\n","protected":false},"author":3,"featured_media":6786,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[516,183],"tags":[4336],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7162"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7162"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7162\/revisions"}],"predecessor-version":[{"id":22277,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7162\/revisions\/22277"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6786"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7162"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7162"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}