{"id":7082,"date":"2016-10-13T16:54:31","date_gmt":"2016-10-13T08:54:31","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=7082"},"modified":"2022-07-25T16:12:21","modified_gmt":"2022-07-25T08:12:21","slug":"%e8%85%ab%e7%98%a4%e5%a3%9e%e6%ad%bb%e5%9b%a0%e6%95%b8%ce%b1tnf%ce%b1%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-cloud-clone-corp%e8%b2%a8%e8%99%9fsea133ra","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=7082","title":{"rendered":"Rat\u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2 |Cloud-Clone corp\u8ca8\u865fSEA133Ra"},"content":{"rendered":"

\u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/strong>|<\/strong>\u8ca8\u865f<\/strong>SEA133Ra<\/strong><\/a><\/span><\/p>\n

ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/p>\n

\u5546\u54c1: \u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2<\/u>|\u8ca8\u865fSEA133Ra\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea615.6-1,000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea66.1pg\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Cloud-Clone corp\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002<\/p>\n

\"SEA133Ra.jpg\"<\/p>\n

\u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Pre-coated, ready to use 96-well strip plate<\/td>\n1<\/td>\nPlate sealer for 96 wells<\/td>\n4<\/td>\n<\/tr>\n
Standard<\/td>\n2<\/td>\nStandard Diluent<\/td>\n1\u00d720mL<\/td>\n<\/tr>\n
Detection Reagent A<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent A<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
Detection Reagent B<\/td>\n1\u00d7120\u00b5L<\/td>\nAssay Diluent B<\/td>\n1\u00d712mL<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n1\u00d79mL<\/td>\nStop Solution<\/td>\n1\u00d76mL<\/td>\n<\/tr>\n
Wash Buffer (30 \u00d7 concentrate)<\/td>\n1\u00d720mL<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u514d\u75ab\u5206\u6790\u6cd5\u7684\u6982\u62ec\u6d41\u7a0b<\/strong><\/p>\n

    \n
  1. Prepare all reagents, samples and standards;<\/li>\n
  2. Add 100\u00b5L standard or sample to each well. Incubate 1 hours at 37\u00b0C;<\/li>\n
  3. Aspirate and add 100\u00b5L prepared Detection Reagent A. Incubate 1 hour at 37\u00b0C;<\/li>\n
  4. Aspirate and wash 3 times;<\/li>\n
  5. Add 100\u00b5L prepared Detection Reagent B. Incubate 30 minutes at 37\u00b0C;<\/li>\n
  6. Aspirate and wash 5 times;<\/li>\n
  7. Add 90\u00b5L Substrate Solution. Incubate 15-25 minutes at 37\u00b0C;<\/li>\n
  8. Add 50\u00b5L Stop Solution. Read at 450nm immediately.<\/li>\n<\/ol>\n

    \u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    Catalog No.<\/td>\nRelated products for research use of Rattus norvegicus (Rat) Organism species<\/td>\nApplications<\/td>\n<\/tr>\n
    APA133Ra01<\/td>\nActive Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nCell culture; Activity Assays; In vivo assays.<\/td>\n<\/tr>\n
    RPA133Ra01<\/td>\nRecombinant Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nSDS-PAGE; WB; ELISA; IP.<\/td>\n<\/tr>\n
    PAA133Ra01<\/td>\nPolyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.<\/td>\n<\/tr>\n
    LAA133Ra81<\/td>\nFITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nIHC;ICC;IF;WB<\/td>\n<\/tr>\n
    LAA133Ra71<\/td>\nBiotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nIHC;WB;ELISA<\/td>\n<\/tr>\n
    MAA133Ra21<\/td>\nMonoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nWB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.<\/td>\n<\/tr>\n
    SEA133Ra<\/td>\nELISA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection<\/td>\n<\/tr>\n
    SCA133Ra<\/td>\nCLIA Kit for Tumor Necrosis Factor Alpha (TNFa)<\/strong><\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u816b\u7624\u58de\u6b7b\u56e0\u6578\u03b1(TNF\u03b1)\u6aa2\u6e2c\u8a66\u5291\u76d2(\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) |\u8ca8\u865fSEA133Ra ELISA Kit for […]<\/p>\n","protected":false},"author":3,"featured_media":5836,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[4355],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7082"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7082"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7082\/revisions"}],"predecessor-version":[{"id":17355,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/7082\/revisions\/17355"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/5836"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7082"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7082"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7082"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}