{"id":6504,"date":"2016-09-09T10:46:28","date_gmt":"2016-09-09T02:46:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=6504"},"modified":"2022-07-26T13:57:09","modified_gmt":"2022-07-26T05:57:09","slug":"lenti-sv40-lentivirus%e7%97%85%e6%af%92%e6%b6%b2-sv40-whole-gene%e8%b2%a8%e8%99%9fg203","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=6504","title":{"rendered":"Lenti-SV40 Lentivirus\u75c5\u6bd2\u6db2 |SV40 whole gene\u8ca8\u865fG203"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6504\"><strong>Lenti-SV40 Lentivirus<\/strong><strong>\u75c5\u6bd2\u6db2 |<\/strong><strong>\u8ca8\u865f<\/strong><strong>G203 \u00a0<\/strong><\/a><\/span><\/p>\n<p><strong>\u5305\u88dd<\/strong><strong>10ml<\/strong> <strong>Lentivirus expressing SV40 whole gene (106 IU\/ml)<\/strong><\/p>\n<p>No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. <a href=\"https:\/\/www.abmgood.com\/SV40-Cell-Immortalization.html\"><br \/>\n\u75c5\u6bd2\u76f8\u95dc\u8cc7\u6599\u8acb\u6309\u6b64pLenti SV40<\/a><\/p>\n<p>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u6c38\u751f\u5316\u7684\u65b9\u6cd5\u3002\u4e00\u7a2e\u662f\u4f7f\u7528\u75c5\u6bd2\u57fa\u56e0\uff0c\u4f8b\u5982\u733f\u7334\u75c5\u6bd240\uff08SV40\uff09T\u6297\u539f\uff0c\u4ee5\u8a98\u5c0e\u6c38\u751f\u5316\u3002 SV40 T\uff08\u5927T\uff09\u6297\u539f\u5df2\u88ab\u8b49\u660e\u662f\u57f9\u990a\u4e2d\u8a31\u591a\u4e0d\u540c\u7d30\u80de\u985e\u578b\u6c38\u751f\u5316\u7684\u6700\u7c21\u55ae\u548c\u6700\u53ef\u9760\u7684\u8a66\u5291\u3002\u5728\u5927\u591a\u6578\u60c5\u6cc1\u4e0b\uff0c\u75c5\u6bd2\u57fa\u56e0\u901a\u904e\u00a0inactivating\u816b\u7624\u6291\u5236\u57fa\u56e0\uff08p53\uff0cRb\u7b49\uff09\u5728\u7d30\u80de\u4e2d\u8a98\u5c0e\u8907\u88fd\u8870\u8001\u72c0\u614b\uff0c\u5be6\u73fe\u6c38\u751f\u5316\u3002\u7814\u7a76\u9084\u8868\u660e\uff0cSV40 T\u6297\u539f\u53ef\u4ee5\u8a98\u5c0e\u611f\u67d3\u7d30\u80de\u4e2d\u7684\u7aef\u7c92\u9176\u6d3b\u6027(\u00a0telomerase activity)\u3002abm\u9084\u63d0\u4f9b\u4e86SV40 T\u6297\u539f\uff08SV40 T antigen-tsA58\uff09\u7684\u6eab\u5ea6\u654f\u611f\u7a81\u8b8a\u9ad4\uff0c\u5176\u7d93\u5e38\u7528\u65bc\u539f\u4ee3\u7d30\u80de\u7684\u689d\u4ef6\u6c38\u751f\u5316\u3002\u7576\u751f\u9577\u572832-34\u00b0C\u7684\u5141\u8a31\u751f\u9577\u6eab\u5ea6\u6642\uff0cSV40 T tsA58\u6162\u75c5\u6bd2\u611f\u67d3\u7684\u7d30\u80de\u8207\u91ce\u751f\u578bSV40 T\u6297\u539f\u6c38\u751f\u5316\u7d30\u80de\u76f8\u4f3c\uff0c\u4f46\u5728\u9650\u5236\u6027\u751f\u9577\u6eab\u5ea6\uff0839-41\u00b0C\uff09\uff0c\u7d30\u80de\u6062\u5fa9\u5230\u975e\u6c38\u751f\u5316\u7d30\u80de\u5f62\u614b\u3002<\/p>\n<table border=\"0\" width=\"100%\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Vector Name<\/td>\n<td>pAdeno-SV40<\/td>\n<\/tr>\n<tr>\n<td>VectorType<\/td>\n<td>Plasmid<\/td>\n<\/tr>\n<tr>\n<td>Antibiotic Information<\/td>\n<td>No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds.<\/td>\n<\/tr>\n<tr>\n<td>Sequencing Primers<\/td>\n<td>Sequencing Primer (621-640)<br \/>\n5`&#8212;CTACAACAAGGCAAGGCTTG&#8212;3`<\/td>\n<\/tr>\n<tr>\n<td>Cloning Sites<\/td>\n<td>EcoRI<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u6764\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u6764 (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u6747\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002\u5b9a\u91cf\u597d\u4e4bLentivirus\uff0c\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3002\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.abmgood.com\/CellImmor\/images\/Cell-Immortalization-Workflow.png\" alt=\"\" width=\"763\" height=\"272\" \/><\/p>\n<p>\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\u3002\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n<p><strong>ABM<\/strong><strong>\u63d0\u4f9b\u4ee5\u4e0b\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u5de5\u5177\uff1a<\/strong><\/p>\n<ul>\n<li>Gene Immortalization (Myc, p53, Rb, Ras) Reagents<\/li>\n<li>HPV E6-E7 Cell Immortalization Reagents<\/li>\n<li>SV40 T antigen Cell Immortalization Reagents<\/li>\n<li>EBV Cell Immortalization Reagents<\/li>\n<li>hTERT Cell Immortalization Reagents<\/li>\n<\/ul>\n<table>\n<tbody>\n<tr>\n<td width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"384\"><u><a href=\"http:\/\/www.abmgood.com\/SV40-Cell-Immortalization.html\"><strong><u>SV40<\/u><\/strong><strong><u>\u91cd\u7d44\u75c5\u6bd2<\/u><\/strong><\/a><\/u><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G203<\/td>\n<td width=\"476\">Lenti-SV40 Lentivirus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G256<\/td>\n<td width=\"476\">Lenti-SV40T Lentivirus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G258<\/td>\n<td width=\"476\">Lenti-SV40 T+t Lentivirus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G210<\/td>\n<td width=\"476\">Adeno-SV40 Adenovirus<\/td>\n<td width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G212<\/td>\n<td width=\"476\">Retro-SV40 Retrovirus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"312\"><u><a href=\"http:\/\/www.abmgood.com\/hTERT-Cell-Immortalization.html\"><strong><u>hTERT<\/u><\/strong><strong><u>\u91cd\u7d44\u75c5\u6bd2<\/u><\/strong><\/a><\/u><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G200<\/td>\n<td width=\"476\">Lenti-hTERT Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G201<\/td>\n<td width=\"476\">Lenti-hTERT Antisense Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G706<\/td>\n<td width=\"476\">Lenti-EF1\u03b1-hTERT Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G205<\/td>\n<td width=\"476\">Adeno-hTERT Virus<\/td>\n<td width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G208<\/td>\n<td width=\"476\">Adeno-hTERT Antisense Virus<\/td>\n<td width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G207<\/td>\n<td width=\"476\">Retro-E1-hTERT Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"384\"><u><a href=\"http:\/\/www.abmgood.com\/Myc-p53-Rb-Ras-Cell-Immortalization.html\"><strong><u>\u764c\u75c7\u57fa\u56e0\u76f8\u95dc\u91cd\u7d44\u75c5\u6bd2<\/u><\/strong><\/a><\/u><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G217<\/td>\n<td width=\"476\">Lenti-Myc-T58A Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G219<\/td>\n<td width=\"476\">Lenti-p53 siRNA Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G221<\/td>\n<td width=\"476\">Lenti-Ras-V12 Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G223<\/td>\n<td width=\"476\">Lenti-Rb siRNA Virus<\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"384\"><strong>\u5176\u4ed6\u764c\u75c7\u76f8\u95dc\u91cd\u7d44\u75c5\u6bd2<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G229<\/td>\n<td width=\"476\"><u><a href=\"http:\/\/www.abmgood.com\/EBV-Cell-Immortalization.html\">EBV Virus<\/a><\/u><\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">G268<\/td>\n<td width=\"476\"><u><a href=\"http:\/\/www.abmgood.com\/HPV-E6-E7-Cell-Immortalization.html\">Lenti-HPV-16 E6\/E7 Virus<\/a><\/u><\/td>\n<td width=\"100\">10ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<\/strong><a href=\"http:\/\/biopioneer.com.tw\/?cat=443\">\u7d30\u80de\u6c61\u67d3\u9632\u6cbb\u5546\u54c1<\/a> | <a href=\"http:\/\/biopioneer.com.tw\/?cat=57\">\u6162\u75c5\u6bd2\u611f\u67d3\u7cfb\u7d71<\/a> | <a href=\"https:\/\/www.abmgood.com\/Cell-Immortalization.html\">Cell Immortalization Reagents<\/a>\u7d30\u80de\u4e0d\u6764\u8a66\u5291 | Lentivirus\u5b9a\u91cf\u4e4b\u75c5\u6bd2\u6db2 | <a href=\"https:\/\/www.abmgood.com\/Growth-Factors-Kinases.html\">Growth Factors &amp; Kinases<\/a>\u751f\u9577\u56e0\u5b50\u53ca\u6fc0\u7d20<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Lenti-SV40 Lentivirus\u75c5\u6bd2\u6db2 |\u8ca8\u865fG203 \u00a0 \u5305\u88dd10ml Lentivirus ex [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3571,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[454,456],"tags":[4498],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6504"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6504"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6504\/revisions"}],"predecessor-version":[{"id":20842,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/6504\/revisions\/20842"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3571"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}