{"id":53971,"date":"2025-11-24T15:36:10","date_gmt":"2025-11-24T07:36:10","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=53971"},"modified":"2025-12-26T11:06:53","modified_gmt":"2025-12-26T03:06:53","slug":"uncoated-%e8%83%b0%e5%b3%b6%e7%b4%a0elisa%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e9%85%b5%e7%b4%a0%e5%85%8d%e7%96%ab%e5%90%b8%e9%99%84%e8%a9%a6%e9%a9%97%e6%b3%95-%e8%b2%a8%e8%99%9fuksa448","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=53971","title":{"rendered":"(Uncoated) \u80f0\u5cf6\u7d20ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9175\u7d20\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) | \u8ca8\u865fUKSA448Ra1"},"content":{"rendered":"

Uncoated \u591a\u76e4\u5f0fELISA\u5546\u54c1\u5168\u65b0\u4e0a\u5e02!!<\/span><\/p>\n

(Uncoated) <\/span><\/span><\/strong>\u80f0\u5cf6\u7d20ELISA<\/strong>\u6aa2\u6e2c\u8a66\u5291\u76d2 (<\/strong>\u9175\u7d20\u514d<\/strong>\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/strong>| <\/strong>\u8ca8\u865fUKSA448Ra1<\/strong><\/span><\/a><\/span><\/p>\n

ELISA Kit DIY Materials for Insulin (INS)<\/span><\/p>\n

<\/p>\n

\u5546\u54c1: \u80f0\u5cf6\u7d20ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2<\/p>\n

Competitive Inhibition ELISA for Antigen Detection \u8ca8\u865fUKSA448Ra1\uff0c\u61c9\u7528\u7269\u7a2e\u70baRattus norvegicus (Rat)\uff1b\u5075\u6e2c\u7684\u9748\u654f\u5ea6123.5-10,000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea650.2pg\/mL, \u53ef\u61c9\u7528\u65bcSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u4e4b\u6a23\u54c1\u4f86\u6e90\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002USCN\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u4e4bELISA (\u9175\u7d20\u514d\u75ab\u5206\u6790\u5957\u7d44\/\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\uff0c\u5c08\u9580\u751f\u7522\u7814\u7a76\u7528\u6aa2\u6e2c\u8a66\u5291\u7684\u751f\u6280\u516c\u53f8\uff0c\u65bc\u7f8e\u570b\u7814\u767c\u548c\u751f\u7522\uff0c\u7522\u54c1\u7dda\u5305\u62ecELISA Kit\u3001CLIA Kit\u3001Antibody\u53carecombinant protein\uff1bELISA\u7522\u54c1\u7cfb\u5217\u8d85\u904e6000\u7a2e\u4ee5\u4e0a\uff0c\u7269\u7a2e\u9f4a\u5168\u5305\u62ecrat\u3001mouse\u3001rabbit\u3001cavia\u3001human\u3001porcine\u3001bovine\u3001simian\u7b49\u591a\u7269\u7a2e\u5546\u54c1\u3002\u54c1\u8cea\u4fdd\u8b49\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3001\u9ad8\u9748\u654f\u5ea6\u8a2d\u8a08ELISA\u3002Uncoated<\/u><\/strong> ELISA<\/u><\/strong>\u578b\u5f0f<\/u><\/strong>\uff1b\u5305\u62ecCapture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u5176\u6bcf\u7d44\u53ef\u9032\u884c10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

\u80f0\u5cf6\u7d20ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)\u5957\u7d44\u5167\u5bb9<\/span><\/p>\n

\u5305\u62ecCapture Antibody, Detection Antibody\u3001Standard\u3001Streptavidin-HRP\u3001TMB Substrate, 96-well Plate\u3002Buffer<\/a>\u7684\u90e8\u4efd\u53e6\u5916\u8cfc\u8cb7\uff0c\u82e5\u70baSandwich ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 1<\/a>\uff0ccompetitive method ELISA\u8cc7\u6599\u8acb\u6309\u6b64Assay Kit DIY Support Pack 2 <\/a>\u5176\u6bcf\u7d44\u53ef\u9032\u884c10\u76e4ELISA\u5206\u6790\u3002<\/p>\n

\u80f0\u5cf6\u7d20ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2\u64cd\u4f5c\u7c21\u55ae\u6d41\u7a0b<\/span><\/p>\n

    \n
  1. Coat the plates with 100\u03bcL per well of working solution of Capture Antibody.incubate overnight at 4\u00b0C or incubate at 37\u00b0C for 2 hours.<\/li>\n
  2. Aspirate and wash 1 time.<\/li>\n
  3. Block the plates with 200 \u03bcL per well of working solution of Blocking Buffer. Incubate at 37\u00b0C for 1.5 hours.<\/li>\n
  4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.<\/li>\n<\/ol>\n

    \u66f4\u591aDIY Uncoated ELISA\u5206\u6790\u5546\u54c1<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Catalog No.<\/td>\nOrganism species: Rattus norvegicus (Rat)<\/td>\nApplications (RESEARCH USE ONLY!)<\/td>\n<\/tr>\n
    UNPA448Ra01<\/td>\nNative Insulin (INS)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
    URPA448Ra01<\/td>\nRecombinant Insulin (INS)<\/a><\/td>\nPositive Control; Immunogen; SDS-PAGE; WB.<\/td>\n<\/tr>\n
    UPAA448Ra01<\/td>\nPolyclonal Antibody to Insulin (INS)<\/a><\/td>\nWB; IHC<\/td>\n<\/tr>\n
    UMAA448Ra21<\/td>\nMonoclonal Antibody to Insulin (INS)<\/a><\/td>\nWB; IHC; ICC; IP.<\/td>\n<\/tr>\n
    UCEA448Ra<\/td>\nELISA Kit for Insulin (INS)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
    UMEA448Ra<\/td>\nMini Samples ELISA Kit for Insulin (INS)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
    UHEA448Ra<\/td>\nHigh Sensitive ELISA Kit for Insulin (INS)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antigen Detection.<\/td>\n<\/tr>\n
    UAEA448Ra<\/td>\nELISA Kit for Anti-Insulin Antibody (Anti-INS)<\/a><\/td>\nEnzyme-linked immunosorbent assay for Antibody Detection.<\/td>\n<\/tr>\n
    UCCA448Ra<\/td>\nCLIA Kit for Insulin (INS)<\/a><\/td>\nChemiluminescent immunoassay for Antigen Detection.<\/td>\n<\/tr>\n
    ULMA448Ra<\/td>\nMultiplex Assay Kit for Insulin (INS) ,etc. by FLIA (Flow Luminescence Immunoassay)<\/a><\/td>\nFLIA Kit for Antigen Detection.<\/td>\n<\/tr>\n
    UKSA448Ra11<\/td>\nELISA Kit DIY Materials for Insulin (INS)<\/a><\/td>\nMain materials for “Do It (ELISA Kit) Yourself”.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Uncoated \u591a\u76e4\u5f0fELISA\u5546\u54c1\u5168\u65b0\u4e0a\u5e02!! (Uncoated) \u80f0\u5cf6\u7d20ELISA\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9175\u7d20\u514d […]<\/p>\n","protected":false},"author":3,"featured_media":53973,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6961],"tags":[7061],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/53971"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=53971"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/53971\/revisions"}],"predecessor-version":[{"id":53991,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/53971\/revisions\/53991"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/53973"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=53971"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=53971"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=53971"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}