{"id":53013,"date":"2025-10-04T16:31:41","date_gmt":"2025-10-04T08:31:41","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=53013"},"modified":"2025-11-03T13:43:53","modified_gmt":"2025-11-03T05:43:53","slug":"cd274%e9%ab%94%e5%85%a7%e6%87%89%e7%94%a8%e6%8a%97%e9%ab%94-in-vivo-antibody-clone-10f-9g2-invivocrown%e8%b2%a8%e8%99%9f-iv0101","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=53013","title":{"rendered":"CD274\u9ad4\u5167\u61c9\u7528\u6297\u9ad4 (In Vivo antibody) | Clone 10F.9G2 InvivoCrown\u8ca8\u865f IV0101"},"content":{"rendered":"

InVivoPro\u7cfb\u5217\u55ae\u682a\u6297\u9ad4 (\u9ad4\u5167\u6297\u9ad4) ->>CD274 (B7-H1\u548cPD-L1)<\/span><\/span><\/span>
\n<\/span><\/span><\/span><\/strong><\/p>\n

InVivoPro Anti-Mouse PD-L1(B7-H1, CD274) in vivo antibody, Clone 10F.9G2<\/span><\/p>\n

CD274\uff0c\u53c8\u7a31\u70baB7-H1\u548cPD-L1\uff0c\u662f\u4e00\u7a2e\u5206\u5b50\u91cf\u70ba40 kD\u7684I\u578b\u8de8\u819c\u86cb\u767d\uff0c\u5c6c\u65bc\u514d\u75ab\u7403\u86cb\u767d\u53d7\u9ad4\u8d85\u5bb6\u65cf(immunoglobulin receptor superfamily)\u4e2d\u7684B7\u5bb6\u65cf\u6210\u54e1(B7 family)\u3002\u5b83\u8868\u9054\u65bcT\u7d30\u80de\u3001B\u7d30\u80de\u3001NK\u7d30\u80de\u3001\u6a39\u7a81\u72c0\u7d30\u80de(dendritic cells)\u3001IFN-\u03b3\u6d3b\u5316\u7684\u5167\u76ae\u7d30\u80de\u548c\u55ae\u6838\u7d30\u80de(monocytes)\u3002\u5167\u6bd2\u7d20\u2264 1.0 EU\/mg, by the LAL method\uff0c\u61c9\u7528\u65bcIn vivo PD-L1 blocked\u3002InVivoPro \u6297\u5c0f\u9f20 PD-1(CD279) \u9ad4\u5167\u6297\u9ad4\uff08clone 29F.1A12\uff09\u5df2\u6210\u70ba\u81e8\u5e8a\u524d\u514d\u75ab\u6cbb\u7642\u7814\u7a76(preclinical immunotherapy research)\u7684\u57fa\u77f3\u3002\u9019\u6b3e\u9ad8\u5ea6\u7279\u7570\u7684\u55ae\u682a\u6297\u9ad4\u7531 BioXCell \u958b\u767c\uff0c\u53ef\u91dd\u5c0d\u5c0f\u9f20 PD-1\u3002 PD-1 \u662f\u4e00\u7a2e\u95dc\u9375\u7684\u514d\u75ab\u6aa2\u67e5\u9ede\u5206\u5b50(immune checkpoint molecule)\uff0c\u53ef\u6291\u5236 T \u7d30\u80de\u53cd\u61c9(T-cell responses)\uff0c\u4f7f\u816b\u7624( tumors)\u548c\u75c5\u539f\u9ad4(pathogens)\u9003\u907f\u514d\u75ab\u6aa2\u6e2c(immune detection)\u3002\u514b\u968629F.1A12\u6e90\u81ea\u5927\u9f20IgG2a\u514b\u9686(rat IgG2a clone)\uff0c\u5728\u56b4\u683c\u7684animal-free conditions\u4e0b\u751f\u7522\uff0c\u78ba\u4fdd\u5353\u8d8a\u7684\u7d14\u5ea6\uff08\u226595%\uff09\u548c\u8d85\u4f4e\u5167\u6bd2\u7d20\u6c34\u5e73\uff08<1 EU\/mg\uff09\u3002\u5b83\u4ee5\u9ad8\u89aa\u548c\u529b\u548c\u7279\u7570\u6027\u8207PD-1\u80de\u5916\u7d50\u69cb\u57df\u7d50\u5408\uff0c\u6709\u6548\u963b\u65b7\u5176\u8207PD-L1\u548cPD-L2\u914d\u9ad4\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u900f\u904e\u963b\u65b7\u6b64\u6291\u5236\u6027\u8a0a\u865f\u901a\u8def\uff0c\u6b64\u6297\u9ad4\u53ef\u91cd\u632fT\u7d30\u80de\u529f\u80fd\uff0c\u589e\u5f37\u5176\u5c0d\u764c\u7d30\u80de\u7684\u7d30\u80de\u6bd2\u6027\u80fd\u529b(cytotoxic ability)\uff0c\u4e26\u589e\u5f37\u6297\u75c5\u6bd2\u514d\u75ab\u529b( antiviral immunity)\u3002<\/p>\n

<\/p>\n

\u5728\u81e8\u5e8a\u524d\u816b\u7624\u5b78(oncology)\u7814\u7a76\u4e2d\uff0cClone 29F.1A12\u5df2\u986f\u793a\u51fa\u986f\u8457\u7684\u7642\u6548\u3002\u5728\u5c0f\u9f20\u9ed1\u8272\u7d20\u7624(melanoma)\u3001\u5927\u8178\u76f4\u8178\u764c(colorectal cancer)\u548c\u4e73\u764c(breast cancer)\u6a21\u578b\u4e2d\uff0c\u6b64\u6297\u9ad4\u6cbb\u7642\u53ef\u986f\u8457\u6291\u5236\u816b\u7624\u751f\u9577(tumor growth suppression)\u3002\u5b83\u4fc3\u9032 CD8+ T \u7d30\u80de\u7684\u6d3b\u5316(activation)\u548c\u589e\u6b96(proliferation)\uff0c\u540c\u6642\u964d\u4f4e\u816b\u7624\u5fae(tumor microenvironment)\u74b0\u5883\u4e2d\u8017\u7aed T \u7d30\u80de\u7684\u6bd4\u4f8b\u3002Clone 29F.1A12\u8207\u5176\u4ed6\u514d\u75ab\u7642\u6cd5\u85e5\u7269\uff08\u5982\u6297 CTLA-4 \u6297\u9ad4\u6216\u514d\u75ab\u523a\u6fc0\u6027\u7d30\u80de\u6fc0\u7d20\uff09\u5408\u4f75\u4f7f\u7528\u6642\uff0c\u53ef\u767c\u63ee\u5354\u540c\u4f5c\u7528\uff0c\u9032\u4e00\u6b65\u589e\u5f37\u6297\u816b\u7624(antitumor)\u53cd\u61c9\u3002\u9664\u4e86\u764c\u75c7\u7814\u7a76(cancer research)\u4e4b\u5916\uff0c\u9019\u7a2e\u6297\u9ad4\u5728\u50b3\u67d3\u75c5\u7814\u7a76( infectious disease studies)\u4e2d\u4e5f\u5177\u6709\u4e0d\u53ef\u4f30\u91cf\u7684\u50f9\u503c\u3002\u5728\u5305\u62ec\u9f20\u9245\u7d30\u80de\u75c5\u6bd2(viral infections)\u548c\u6dcb\u5df4\u7403\u8108\u7d61\u53e2\u8166\u819c\u708e\u75c5\u6bd2(lymphocytic choriomeningitis virus)\u5728\u5167\u7684\u75c5\u6bd2\u611f\u67d3\u6a21\u578b\u4e2d\uff0cClone 29F.1A12\u53ef\u6062\u5fa9\u6297\u75c5\u6bd2T\u7d30\u80de\u6d3b\u6027\uff0c\u4fc3\u9032\u75c5\u6bd2\u6e05\u9664\u4e26\u9810\u9632\u6162\u6027\u611f\u67d3\u7684\u767c\u5c55\u3002\u5b83\u9084\u6709\u52a9\u65bc\u95e1\u660ePD-1\u5728\u6301\u7e8c\u6027\u75c5\u6bd2\u611f\u67d3(viral infections)\u671f\u9593\u514d\u75ab\u8870\u7aed\u4e2d\u7684\u4f5c\u7528\u3002Clone 29F.1A12 \u7684\u6027\u80fd\u5df2\u901a\u904e\u591a\u7a2e\u5be6\u9a57\u65b9\u6cd5\u5f97\u5230\u56b4\u683c\u9a57\u8b49\u3002\u6d41\u5f0f\u7d30\u80de\u5100( Flow cytometry )\u8b49\u5be6\u5176\u8207PD-1+ T\u7d30\u80de\u7279\u7570\u6027\u7d50\u5408\uff0c\u800c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u67d3\u8272(immunohistochemistry)\u5247\u53ef\u89c0\u5bdf\u5230\u7d44\u7e54\u4e2dPD-1\u7684\u8868\u9054\u3002\u85e5\u7269\u52d5\u529b\u5b78\u7814\u7a76\u5df2\u78ba\u5b9a\u6700\u4f73\u7d66\u85e5\u65b9\u6848\uff0c\u901a\u5e38\u70ba intraperitoneal administration \u7d66\u85e5\uff0c\u5291\u91cf\u70ba2.5-7.5 mg\/kg\uff0c\u6bcf\u9031\u5169\u6b21\u3002<\/p>\n

CD274\u9ad4\u5167\u61c9\u7528\u6297\u9ad4(In Vivo antibody) | Clone: 10F.9G2 InvivoCrown<\/strong>\u8ca8\u865f IV0101<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/span><\/strong><\/span><\/p>\n

InVivoPro\u81f4\u529b\u65bc\u70ba\u79d1\u5b78\u5bb6\u63d0\u4f9b\u7d14\u5ea6\u9ad8\u3001\u9069\u5408\u7814\u7a76\u9818\u57df\u7684\u9ad4\u5167\u61c9\u7528\u6297\u9ad4(In Vivo antibody)\uff0c\u4e0d\u65b7\u64f4\u5c55\u7684\u7522\u54c1\u7d44\u5408\u5305\u542b 20 \u591a\u7a2e\u9ad8\u54c1\u8cea\u55ae\u682a\u6297\u9ad4 (MAb)\uff0c\u6bcf\u7a2e\u90fd\u7d93\u904e\u56b4\u683c\u7684\u9ad4\u5167\u61c9\u7528( in vivo applications)\u9a57\u8b49\u3002\u9019\u4e9b\u7d93\u904e\u79d1\u5b78\u8868\u5fb5\u7684\u8a66\u5291\uff0c\u540c\u6642\u53ef\u7acb\u5373\u63d0\u4f9b\u6beb\u514b\u81f3\u6578\u514b\u7684\u91cf\u7d1a\uff0c\u4e26\u7531\u82f1\u570b\u7684\u5feb\u901f\u914d\u9001\u63d0\u4f9b\u652f\u6301\uff0c\u4ee5\u78ba\u4fdd\u53ef\u9760\u7684\u4f9b\u61c9\u93c8\u9023\u7e8c\u6027\u3002\u4e3b\u8981\u7522\u54c1\u5305\u62ec\uff1a\u91dd\u5c0d\u7279\u5b9a\u8868\u4f4d( epitopes )\u7684\u963b\u65b7\u6027 MAb\u3001\u7528\u65bc\u6a5f\u5236\u85e5\u7406\u5b78\u7814\u7a76\u7684\u529f\u80fd\u6027 MAb (Functional MAbs) \u548c\u751f\u7269\u4eff\u88fd\u85e5\u5c0d\u7167(biosimilar controls)\u3001 \u91dd\u5c0d\u7269\u7a2e\u6709\u7279\u7570\u6027\u7684\u6297\u9f20\u8a66\u5291(anti-mouse reagents\u200b\u200b)\uff0c\u7d93\u904e\u512a\u5316\u4ee5\u6d88\u9664\u4ea4\u53c9\u53cd\u61c9\u6df7\u96dc(cross-reactivity confounders)\u56e0\u7d20\u3002\u6240\u6709\u7522\u54c1\u5747\u7d93\u904e\u4e09\u7d1a\u54c1\u8cea\u4fdd\u8b49(\u200b\u200bthree-tier quality assurance)\uff0c\u78ba\u4fdd\u5728\u81e8\u5e8a\u524d\u6a21\u578b(preclinical models.)\u4e2d\u7684\u53ef\u91cd\u8907\u6027\u3002<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Catalog<\/td>\nIV0101<\/td>\n<\/tr>\n
Product Name<\/td>\nInVivoPro Anti-Mouse\u00a0PD-L1(B7-H1, CD274) in vivo antibody, Clone 10F.9G2<\/td>\n<\/tr>\n
Size<\/td>\n1mg\/5mg\/25mg\/50mg\/100mg<\/td>\n<\/tr>\n
Isotype<\/td>\nRat IgG2b \u03ba<\/td>\n<\/tr>\n
Clone<\/td>\n10F.9G2<\/td>\n<\/tr>\n
Target<\/td>\nPD-L1<\/td>\n<\/tr>\n
Other Names<\/td>\nProgrammed cell death ligand 1\uff0cB7-H1\uff0cCD274\uff0cPD-L1<\/td>\n<\/tr>\n
Isotype Control Catalog Code<\/td>\nIV0106 or Rat IgG2b Isotype Control<\/td>\n<\/tr>\n
Dilution Buffer<\/td>\nPBS, pH 7.2, contains no stabilizers or preservatives<\/td>\n<\/tr>\n
Reactivity<\/td>\nMouse<\/td>\n<\/tr>\n
Host Species<\/td>\nRat<\/td>\n<\/tr>\n
How much antibody to use\u00a0in vivo<\/td>\n200-500 \u03bcg per mouse; or 10 mg\/kg. This range is based off the most recent publication data using the 10F.9G2 clone in vivo. Each investigator should\u00a0 determine their own optimal working dilution for specific applications.<\/td>\n<\/tr>\n
Background(ichorbio)<\/td>\nCD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-\u03b3 activated endothelial cells, and monocytes.<\/td>\n<\/tr>\n
Applications<\/td>\nIn vivo PD-L1 blocked<\/td>\n<\/tr>\n
Purification<\/td>\nprotein A or G<\/td>\n<\/tr>\n
Storage<\/td>\nThis antibody is stable for at least 2 months when stored at 2-8\u00b0C. For long term storage, aliquot in working volumes without diluting and store at -20\u00b0C or -80\u00b0C. Avoid repeated freeze thaw cycles.<\/td>\n<\/tr>\n
Shipping<\/td>\n2-8\u00b0C with blue ice<\/td>\n<\/tr>\n
Concentration<\/td>\nLot specific, generally \u2265 5.0 mg\/ml<\/td>\n<\/tr>\n
Shelf Life<\/td>\n12 months from the date of receipt if stored as recommended<\/td>\n<\/tr>\n
Formulation<\/td>\nPBS Buffer, PH 7.2, with no carrier protein, or preservatives.<\/td>\n<\/tr>\n
Sterility<\/td>\n0.2 \u03bcM filtered<\/td>\n<\/tr>\n
Endotoxin<\/td>\n\u2264 1.0 EU\/mg, by the LAL method<\/td>\n<\/tr>\n
Purity<\/td>\n99%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\n
\n

InvivoCrown<\/span><\/strong><\/p>\n<\/div>\n<\/div>\n

https:\/\/www.crownzz.com\/<\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
InvivoCrown \u7522\u54c1\u8ca8\u865f<\/td>\nInvivoCrown \u54c1\u9805<\/td>\n\u7db2\u7ad9\u8cc7\u6599<\/td>\n\u64cd\u4f5c\u624b\u518a<\/td>\n<\/tr>\n
IV0100<\/td>\nInVivoPro Anti-Mouse\u00a0PD-1(CD279) in vivo antibody, Clone RMP1-14<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0101<\/td>\nInVivoPro Anti-Mouse\u00a0PD-L1(B7-H1, CD274) in vivo antibody, Clone 10F.9G2<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0102<\/td>\nInVivoPro Anti-Mouse CD4 in vivo antibody, Clone GK1.5<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0103<\/td>\nInVivoPro Anti-Mouse Ly6G in vivo antibody, Clone 1A8<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0104<\/td>\nInVivoPro Anti-Mouse CD8\u03b1 in vivo antibody, Clone 2.43<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0105<\/td>\nInVivoPro Rat IgG2a in vivo Isotype control<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0106<\/td>\nInVivoPro Rat IgG2b in vivo Isotype control<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0107<\/td>\nInVivoPro Anti-Mouse\u00a0PD-1(CD279) in vivo antibody, Clone 29F.1A12<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0109<\/td>\nInVivoPro Anti-Mouse CTLA-4 (CD152) in vivo antibody, Clone 9D9<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0110<\/td>\nInVivoPro Anti-Mouse TNF\u03b1 in vivo antibody, Clone TN3-19.12<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0111<\/td>\nInVivoPro Anti-Mouse NK1.1(CD161) Antibody in vivo antibody, Clone PK136<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0112<\/td>\nInVivoPro Anti-Mouse CTLA-4 (CD152) in vivo antibody, Clone 9H10<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0113<\/td>\nInVivoPro Anti-Mouse Ly6G\/Ly6C (Gr-1) in vivo antibody, Clone RB6-8C5<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0114<\/td>\nInVivoPro Anti-Mouse CSF1R (CD115) in vivo antibody, Clone AFS98<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0116<\/td>\nInVivoPro Anti-Mouse CD40 in vivo antibody, Clone FGK45<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0117<\/td>\nInVivoPro Anti-Mouse CD8\u03b2 (Lyt 3.2) in vivo antibody, Clone 53-5.8<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0118<\/td>\nInVivoPro Anti-Mouse IFN\u03b3 in vivo antibody, Clone XMG1.2<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0119<\/td>\nInVivoPro Anti-Mouse CD8\u03b1 in vivo antibody, Clone 53-6.7<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0120<\/td>\nInVivoPro Anti-Mouse CD28 in vivo antibody, Clone 37.51<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n
IV0121<\/td>\nInVivoPro Anti-Mouse CD3\u03b5 in vivo antibody, Clone 145-2C11<\/td>\n\u7db2\u7ad9\u9023\u7d50<\/td>\n\u4e0b\u8f09<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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