{"id":52683,"date":"2025-09-01T17:33:04","date_gmt":"2025-09-01T09:33:04","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=52683"},"modified":"2025-11-15T13:40:17","modified_gmt":"2025-11-15T05:40:17","slug":"%e4%b8%80%e6%b0%a7%e5%8c%96%e6%b0%ae%ef%bc%88no%ef%bc%89%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-colorimetric-nitric-oxide-no-assay-kit-apexbio%e8%b2%a8%e8%99%9fk2107","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=52683","title":{"rendered":"\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0 | Colorimetric Nitric Oxide (NO) Assay Kit APExBIO\u8ca8\u865fK2107"},"content":{"rendered":"

APExBIO\u7cfb\u5217\u5546\u54c1->><\/span><\/span><\/span>\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/span><\/span>
\n<\/span><\/span><\/span><\/strong><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291 (Small Molecule Inhibitors)\/\u6d3b\u5316\u5291 (Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0 | Colorimetric Nitric Oxide (NO) Assay Kit APExBIO\u8ca8\u865fK2107<\/a><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/span><\/strong><\/span><\/p>\n

A kit for the determination of NO in samples by an optimized Griess Reagent<\/p>\n

\u4e00\u6c27\u5316\u6c2e (Nitric oxide, NO) \u662f\u751f\u7269\u9ad4\u4e2d\u7684\u95dc\u9375\u8a0a\u865f\u5206\u5b50\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u795e\u7d93\u7cfb\u7d71(neurological)\u3001\u5faa\u74b0\u7cfb\u7d71(circulatory)\u3001\u6d88\u5316\u7cfb\u7d71(digestive)\u3001\u547c\u5438\u7cfb\u7d71(respiratory)\u548c\u6ccc\u5c3f\u751f\u6b96\u7cfb\u7d71(genitourinary systems)\u4e2d\uff0c\u5728\u751f\u7406(physiological)\u548c\u75c5\u7406(pathological)\u904e\u7a0b\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u4e00\u6c27\u5316\u6c2e (Nitric oxide, NO) \u53ef\u88ab\u6c27\u5316\u70ba\u4e9e\u785d\u9178\u9e7d\u548c\u785d\u9178\u9e7d\u3002\u9593\u63a5\u6aa2\u6e2c \u4e00\u6c27\u5316\u6c2e (Nitric oxide, NO) \u7684\u5e38\u7528\u65b9\u6cd5\u662f\u6e2c\u5b9a\u5176\u7a69\u5b9a\u7684\u6c27\u5316\u7522\u7269\u4e9e\u785d\u9178\u9e7d\u3002\u6bd4\u8272\u6cd5\u4e00\u6c27\u5316\u6c2e (Nitric oxide, NO) \u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u4f7f\u7528\u512a\u5316\u7684 Griess \u8a66\u5291\u6e2c\u5b9a\u57f9\u990a\u7d30\u80de(cultured cells)\u3001\u7d44\u7e54(tissues)\u3001\u8840\u6e05(serum)\u3001\u8840\u6f3f( plasma)\u548c\u5c3f\u6db2(urine)\u7b49\u6a23\u672c\u4e2d NO \u542b\u91cf\u7684\u8a66\u5291\u76d2\u3002\u5176\u539f\u7406\u662f Griess \u8a66\u5291\u53ef\u8207\u4e9e\u785d\u9178\u9e7d(nitrite)\u53cd\u61c9\uff0c\u751f\u6210\u7684\u7522\u7269\u5728 540 nm \u8655\u5177\u6709\u7279\u7570\u6027\u5438\u5149\u5ea6\u3002\u5438\u5149\u5ea6\u8207\u4e00\u6c27\u5316\u6c2e (Nitric oxide, NO) \u542b\u91cf\u5448\u6b63\u76f8\u95dc\u3002\u8a72\u8a66\u5291\u76d2\u7d93\u904e\u512a\u5316\uff0c\u53ef\u6aa2\u6e2c\u4f4e\u81f3 1 \u03bcM \u7684 NO\uff0c\u4e26\u5728 1-100 \u03bcM \u7bc4\u570d\u5167\u5177\u6709\u826f\u597d\u7684\u7dda\u6027\u3002<\/p>\n

<\/p>\n

\u6a19\u6e96\u54c1\u7a00\u91cb\uff081 M NaNO2\uff09\uff1a\u4ee5\u7121\u83cc\u6c34\u5c0d 1 M NaNO2 \u9032\u884c\u68af\u5ea6\u7a00\u91cb\uff0c\u7528\u65bc\u5f8c\u7e8c\u6a19\u6e96\u66f2\u7dda\u7684\u88fd\u5099\u3002\u6211\u5011\u5efa\u8b70\u68af\u5ea6\u7a00\u91cb\u81f3 0\u30011\u30012\u30015\u300110\u300120\u300140\u300160\u3001100 \u03bcM\uff0c\u6bcf\u7a2e\u68af\u5ea6\u81f3\u5c11 200 \u03bcL\u3002Griess Reagent\u7684\u914d\u88fd\uff1a\u5c07\u9069\u91cf\u7684Griess Reagent A\u548cGriess Reagent B\u4ee51:1\u7684\u6bd4\u4f8b\u6df7\u5408\uff0c\u5373\u53ef\u914dGriess Reagent\u3002\u8acb\u6e96\u5099\u8db3\u5920\u7684\u8a66\u5291\u4ee5\u4f9b\u7acb\u5373\u9032\u884c\u5be6\u9a57\u3002\u6bcf\u6b21\u914d\u88fdGriess Reagent\u6642\uff0c\u8acb\u52d9\u5fc5\u65b0\u9bae\u914d\u88fd\u3002\u914d\u88fd\u904e\u7a0b\u9700\u907f\u5149\u3002<\/p>\n

\u6a23\u54c1\u88fd\u5099\uff1a\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u53ef\u76f4\u63a5\u53d6\u6a23\u6aa2\u6e2c\u3002\u5982\u6709\u6c89\u6fb1\uff0c\u5efa\u8b70\u96e2\u5fc3\u5f8c\u53d6\u6f84\u6e05\u4e0a\u6e05\u9032\u884c\u6aa2\u6e2c\u3002\u7d30\u80de\u6216\u7d44\u7e54\u6a23\u672c\u53ef\u4f7f\u7528\u5feb\u901f\u51cd\u878d\u88c2\u89e3\u6cd5\u6216\u4ee5\u88c2\u89e3\u7de9\u885d\u6db2\uff08\u5efa\u8b70\u4f7f\u7528WB\u548cIP\u7d30\u80de\u88c2\u89e3\u7de9\u885d\u6db2\uff09\u88c2\u89e3\uff0c\u7136\u5f8c\u96e2\u5fc3\u53d6\u4e0a\u6e05\u9032\u884c\u6aa2\u6e2c\u3002<\/p>\n

\u6aa2\u6e2c\u7cfb\u7d71\u914d\u88fd\uff1a\u5c07\u5fae\u5b54\u677f\uff08\u6a23\u54c1\u5bb9\u91cf\u81f3\u5c11\u70ba300 \u03bcL\uff09\u4e2d\u7684\u6bcf\u5b54\u4f9d\u4e0b\u5217\u8981\u6c42\u6df7\u52fb\uff0c\u52a0\u5165\u6642\u8acb\u6ce8\u610f\u4e0d\u8981\u7522\u751f\u6c23\u6ce1\uff0c\u4e26\u6df7\u52fb\u5f8c\u5ba4\u6eab\u5b75\u80b230\u5206\u9418\u3002\u82e5\u6240\u7528\u5fae\u5b54\u677f\u9ad4\u7a4d\u5c0f\u65bc\u672c\u9ad4\u7cfb\uff0c
\n\u53ef\u5c07\u4e0b\u8868\u4e2d\u7684\u8a66\u5291\u7528\u91cf\u6e1b\u534a\uff0c\u5373\u9ad4\u4fc2\u7528\u91cf\u6e1b\u5c11\u81f3150 \u03bcL\uff0c\u6aa2\u6e2c\uff1a\u5b75\u80b2\u5b8c\u6210\u5f8c\uff0c\u4ee5\u9176\u6a19\u5100\u5728540 nm\u8655\u6e2c\u5b9a\u5438\u5149\u5ea6\uff0c \u4e26\u6839\u64da\u6a19\u6e96\u66f2\u7dda\u4f30\u7b97\u6a23\u54c1\u4e2d\u7684NO\u542b\u91cf\u3002<\/span><\/p>\n

<\/p>\n

1. Griess Reagent B \u89e3\u51cd\u5f8c\u5982\u6709\u6c89\u6fb1\uff0c\u53ef\u6416\u52fb\u4fc3\u9032\u6eb6\u89e3\uff0c\u5f85\u5b8c\u5168\u6eb6\u89e3\u5f8c\u65b9\u53ef\u6b63\u5e38\u4f7f\u7528\u3002
\n2. \u672c\u8a66\u5291\u76d2\u4e2d\u7684\u6eb6\u6db2\u8acb\u52ff\u9577\u6642\u9593\u66b4\u9732\u5728\u7a7a\u6c23\u4e2d\uff0c\u5426\u5247\u53ef\u80fd\u5931\u6548\u3002
\n3. \u5982\u9700\u4f7f\u7528\u88c2\u89e3\u7de9\u885d\u6db2\u88c2\u89e3\u7d30\u80de\uff0c\u5efa\u8b70\u4f7f\u7528 WB \u548c IP \u7d30\u80de\u88c2\u89e3\u7de9\u885d\u6db2\uff08\u8ca8\u865f K1123\uff09\uff0c\u800c\u975e RIPA \u88c2\u89e3\u7de9\u885d\u6db2\u3002
\n4. \u672c\u7522\u54c1\u5c0d\u4eba\u9ad4\u6709\u5bb3\uff0c\u64cd\u4f5c\u6642\u8acb\u6ce8\u610f\u9632\u8b77\u3002
\n5. \u70ba\u4e86\u60a8\u7684\u5b89\u5168\u8207\u5065\u5eb7\uff0c\u5be6\u9a57\u904e\u7a0b\u4e2d\u8acb\u7a7f\u8457\u5be6\u9a57\u670d\u4e26\u6234\u4e0a\u624b\u5957\u3002
\n6. \u50c5\u4f9b\u79d1\u5b78\u7814\u7a76\u4f7f\u7528\uff0c\u4e0d\u53ef\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7\u6216\u81e8\u5e8a\u8a66\u9a57\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\n
\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/p>\n<\/div>\n<\/div>\n

https:\/\/www.apexbt.com<\/a><\/span><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

APExBIO\u7cfb\u5217\u5546\u54c1->>\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0 APExBIO Technology L […]<\/p>\n","protected":false},"author":3,"featured_media":49564,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368,7029],"tags":[6985],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/52683"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=52683"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/52683\/revisions"}],"predecessor-version":[{"id":53638,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/52683\/revisions\/53638"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/49564"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=52683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=52683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=52683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}