{"id":51622,"date":"2025-06-26T11:30:44","date_gmt":"2025-06-26T03:30:44","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=51622"},"modified":"2025-08-11T17:49:18","modified_gmt":"2025-08-11T09:49:18","slug":"tunel%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-dab-servicebio-%e8%b2%a8%e8%99%9fg1507-50t","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=51622","title":{"rendered":"TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 (DAB) | SERVICEBIO \u8ca8\u865fG1507-50T"},"content":{"rendered":"<p><span style=\"font-size: 14pt;\"><strong><span style=\"color: #800080;\">SERVICEBIO\u7cfb\u5217\u7522\u54c1-<span style=\"color: #ff6600;\"> TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 (DAB\u5448\u8272)<\/span><\/span><\/strong><\/span><\/p>\n<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=51622&amp;preview=true\">DAB\uff08SA-HRP\uff09TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 SERVICEBIO \u8ca8\u865fG1507-50T<\/a><\/span><\/strong><strong><span style=\"color: #33cccc; font-size: 12pt;\"><br \/>\n<\/span><\/strong><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u7522\u54c1\u4ecb\u7d39:<\/strong><\/span><\/p>\n<p>\u7d30\u80de\u51cb\u4ea1(apoptosis)\u6642\u67d3\u8272\u9ad4DNA(chromosomal DNA)\u7684\u65b7\u88c2\u662f\u4e00\u500b\u6f38\u9032\u7684\u904e\u7a0b\u3002\u67d3\u8272\u9ad4DNA( Chromosomal DNA )\u9996\u5148\u5728\u5167\u6e90\u6027\u6838\u9178\u9176\u6c34\u89e3\u9176(endogenous nuclease hydrolase)\u7684\u4f5c\u7528\u4e0b\u964d\u89e3\u70ba50\uff5e300 KB\u7684\u5927\u7247\u6bb5\uff0c\u96a8\u5f8c\u7d0430%\u7684\u67d3\u8272\u9ad4DNA\u5728Ca2+\u548cMg2+\u4f9d\u8cf4\u6027\u6838\u9178\u5167\u5207\u9176(ca2+ and mg2+ dependent endonuclease) \u7684\u4f5c\u7528\u4e0b\u5728\u6838\u5c0f\u9ad4\u55ae\u5143\u4e4b\u9593\u96a8\u6a5f\u5207\u5272\uff0c\u5f62\u6210180\uff5e200 BP\u7684\u6838\u5c0f\u9ad4DNA\u805a\u5408\u7269\u3002\u56e0\u6b64\uff0c\u5728\u7d30\u80de\u51cb\u4ea1\u5f8c\u671f\uff0cDNA\u6703\u964d\u89e3\u70ba180\uff5e200 BP\u7684\u7247\u6bb5\uff0c\u65b7\u88c2\u7684\u57fa\u56e0\u7d44DNA\u4e0a\u6703\u6709\u5927\u91cf3&#8242;-OH\u672b\u7aef\u66b4\u9732\u3002\u672b\u7aef\u812b\u6c27\u6838\u7cd6\u6838\u82f7\u9178\u8f49\u79fb\u9176\uff08TDT\uff09\u662f\u4e00\u7a2e\u4e0d\u4f9d\u8cf4\u6a21\u677f\u7684DNA\u805a\u5408\u9176( DNA polymerase)\uff0c\u80fd\u5920\u50ac\u5316\u812b\u6c27\u6838\u82f7\u9178(deoxynucleotides )\u8207\u65b7\u88c2\u7684DNA\u5206\u5b50\u76843&#8242;-OH\u672b\u7aef\u7d50\u5408\u3002\u56e0\u6b64\uff0cTUNEL\uff08TDT\u4ecb\u5c0e\u7684dUTP\u7f3a\u53e3\u672b\u7aef\u6a19\u8a18\uff09\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u6aa2\u6e2c\u7d44\u7e54\u7d30\u80de\u51cb\u4ea1\u5f8c\u671f\u767c\u751f\u7684\u6838DNA\u65b7\u88c2\u3002\u5176\u539f\u7406\u662f\u5728TDT\u9176\u7684\u4f5c\u7528\u4e0b\uff0c\u5c07\u751f\u7269\u7d20\u6a19\u8a18\u7684dUTP\uff08\u751f\u7269\u7d20dUTP\uff09\u6dfb\u52a0\u5230\u57fa\u56e0\u7d44DNA\u65b7\u88c2\u6642\u66b4\u9732\u76843\u00b4-OH\u672b\u7aef\uff0c\u7136\u5f8c\u7528\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u6a19\u8a18\u7684\u93c8\u9ef4\u89aa\u548c\u7d20\uff08SA HRP\uff09\u6aa2\u6e2c\u751f\u7269\u7d20\u6a19\u8a18\u7684DNA\u672b\u7aef\u3002\u6700\u5f8c\u52a0\u5165HRP\u7684\u5e95\u7269\u6df7\u5408\u7269\uff08DAB\uff09\u9032\u884c\u986f\u8272\u53cd\u61c9\uff0c\u51cb\u4ea1\u7d30\u80de\u7684\u7d30\u80de\u6838\u67d3\u6210\u68d5\u8272\uff0c\u53ef\u7528\u4e00\u822c\u5149\u5b78\u986f\u5fae\u93e1\u6aa2\u6e2c\u3002\u6b64\u8a66\u5291\u76d2\u61c9\u7528\u7bc4\u570d\u5ee3\u6cdb\uff0c\u9069\u7528\u65bc\u77f3\u881f\u7d44\u7e54\u5207\u7247(paraffin tissue sections)\u3001\u51b0\u51cd\u7d44\u7e54\u5207\u7247(frozen tissue sections)\u3001\u7d30\u80de\u722c\u7247(cell climbing slides)\u3001\u7d30\u80de\u62b9\u7247(cell smears)\u7b49\u6a23\u672c\u4e2d\u7684\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/06\/3DAB%EF%BC%88SA-HRP%EF%BC%89TUNEL%E7%B4%B0%E8%83%9E%E5%87%8B%E4%BA%A1%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-SERVICEBIO-%E8%B2%A8%E8%99%9FG1507-50T.png\" \/><\/p>\n<div class=\"display-inline-block\"><button class=\"cursor-pointer text-no-underline bg-transparent border-0 padding-0 text-left margin-0 text-normal text-primary\" type=\"button\" aria-controls=\"journal_context_menu\" aria-expanded=\"false\">Cell Death Dis<\/button><\/div>\n<p>\u5f15\u7528\u6587\u737b: \u51fa\u81ea2022 May 12;13(5):451. doi: <a class=\"usa-link usa-link--external\" href=\"https:\/\/doi.org\/10.1038\/s41419-022-04914-6\" target=\"_blank\" rel=\"noopener noreferrer\" data-ga-action=\"click_feat_suppl\">10.1038\/s41419-022-04914-6<\/a><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/06\/4-TUNEL%E7%B4%B0%E8%83%9E%E5%87%8B%E4%BA%A1%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-DAB-SERVICEBIO-%E8%B2%A8%E8%99%9FG1507-50T.png\" \/><\/p>\n<p>\u5f15\u7528\u6587\u737b: \u51fa\u81ea Int J Mol Med. 2025 Apr;55(4):65. doi: 10.3892\/ijmm.2025.5506. Epub 2025 Feb 21.<\/p>\n<p><span style=\"font-size: 12pt; color: #993300;\"><strong>Preparation<\/strong><\/span><\/p>\n<p>1. PBS\u78f7\u9178\u9e7d\u7de9\u885d\u6db2\uff08\u5efa\u8b70G0002\uff09\u3002<\/p>\n<p>2. \u56fa\u5b9a\u6db2\uff1a4%\u591a\u805a\u7532\u919b\u6eb6\u65bcPBS\uff0cpH 7.4\uff08\u5efa\u8b70G1101\uff09\u3002<\/p>\n<p>3. \u7834\u819c\u5291\uff1a0.1%-0.5% Triton X-100\uff08\u5efa\u8b70G1204\uff09\u3002<\/p>\n<p>4. \u904e\u6c27\u5316\u6c2b\u5c01\u5b54\u6db2\uff1a3% H2O2\uff0c\u7528PBS\u914d\u88fd\u3002<\/p>\n<p>5. \u5982\u9700\u67d3\u8272\u7d30\u80de\u6838\uff0c\u9700\u81ea\u884c\u6e96\u5099\u8607\u6728\u7cbe\u67d3\u6db2\uff08\u5efa\u8b70G1004\uff09\u3001\u8607\u6728\u7cbe\u5206\u5316\u6db2\uff08\u5efa\u8b70G0139\uff09\u4ee5\u53ca\u8607\u6728\u7cbe\u85cd\u5316\u6db2\uff08\u5efa\u8b70G1004\uff09\u3002<\/p>\n<p>6. \u81ea\u884c\u914d\u88fdDAB\u986f\u8272\u5291\uff08\u5efa\u8b70G1212\uff09\u3001\u6b63\u4e01\u9187\u3001\u4e8c\u7532\u82ef\u3001\u4e2d\u6027\u6a39\u81a0\u7b49\u3002<\/p>\n<p>7. \u64cd\u4f5c\u6642\u8acb\u7a7f\u8457\u6e2c\u8a66\u670d\u4e26\u6234\u4e0a\u4e00\u6b21\u6027\u624b\u5957\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #993300;\"><strong>Assay Protocol \/ Procedures<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt; color: #ff9900;\"><strong>1.\u00a0Sample preparation<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt; color: #ff9900;\"><strong>A.\u00a0Paraffin embedded tissue section<\/strong><\/span><\/p>\n<p>1) \u5c07\u77f3\u881f\u7d44\u7e54\u5207\u7247\u5728\u5ba4\u6eab\u4e0b\u6d78\u6ce1\u5728\u4e8c\u7532\u82ef\u4e2d5-10\u5206\u9418\uff0c\u91cd\u89073\u6b21\uff1b\u7136\u5f8c\u6d78\u6ce1\u5728\u7121\u6c34\u4e59\u9187\u4e2d5\u5206\u9418\uff0c\u91cd\u89072\u6b21\uff1b\u6700\u5f8c\uff0c\u5206\u5225\u6d78\u6ce1\u5728\u68af\u5ea6\u4e59\u9187\uff0885%\u300175%\uff09\u548c\u96d9\u84b8\u6c34\u4e2d\uff0c\u54045\u5206\u9418\uff1b<\/p>\n<p>2) \u7528PBS\u8f15\u8f15\u6f64\u6fd5\u5207\u7247\uff0c\u4e26\u5438\u53bb\u6a23\u54c1\u5468\u570d\u591a\u9918\u7684\u6db2\u9ad4\u3002\u7528PAP\u7b46\u6cbf\u8457\u7d44\u7e54\u5916\u5468\u8f2a\u5ed3\uff0c\u5728\u8ddd\u96e2\u7d44\u7e542-3\u6beb\u7c73\u8655\u756b\u4e00\u500b\u5c0f\u5713\u5708\uff0c\u4ee5\u4fbf\u5f8c\u7e8c\u9032\u884c\u901a\u900f\u6027\u8655\u7406\u548c\u5e73\u8861\u6a19\u8a18\u3002\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u4e0d\u8981\u8b93\u6a23\u54c1\u4e7e\u71e5\uff0c\u5c07\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u653e\u5165\u6fd5\u76d2\u4e2d\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\uff1b<\/p>\n<p>3) \u914d\u88fd\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\uff1a\u5c07\u86cb\u767d\u9176K\uff08200 \u00b5g\/ml\uff09\u539f\u6db2\u4ee5PBS\u4f5c\u70ba\u7a00\u91cb\u5291\uff0c\u4ee51:9\u7684\u9ad4\u7a4d\u6bd4\u7a00\u91cb\uff0c\u4f7f\u7d42\u6fc3\u5ea6\u70ba20 \u03bcg\/mL\uff1b<\/p>\n<p>4) \u6bcf\u4efd\u6a23\u672c\u52a0\u5165100 \u03bcL\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\uff0c\u5b8c\u5168\u8986\u84cb\u7d44\u7e54\uff0c37\u2103\u5b75\u80b220\u5206\u9418\uff1b<br \/>\n\u6ce8\u610f\uff1a\u86cb\u767d\u9176K\u8655\u7406\u4e3b\u8981\u6709\u52a9\u65bc\u5f8c\u7e8c\u6b65\u9a5f\u4e2d\u67d3\u8272\u8a66\u5291\u5728\u7d44\u7e54\u548c\u7d30\u80de\u4e2d\u7684\u6ef2\u900f\u6027\u3002\u5b75\u80b2\u6642\u9593\u904e\u9577\u6216\u904e\u77ed\u90fd\u6703\u5f71\u97ff\u5f8c\u7e8c\u6a19\u8a18\u6548\u7387\u3002\u70ba\u4e86\u7372\u5f97\u66f4\u4f73\u7d50\u679c\uff0c\u53ef\u4ee5\u512a\u5316\u86cb\u767d\u9176K\u7684\u5b75\u80b2\u6642\u9593\u3002<\/p>\n<p>5) \u7528PBS\u6eb6\u6db2\u6d78\u6ce1\u6e05\u6d17\u6a23\u54c13\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff08\u86cb\u767d\u9176K\u9700\u6e05\u6d17\uff0c\u5426\u5247\u6703\u5e79\u64fe\u5f8c\u7e8c\u6a19\u8a18\u53cd\u61c9\uff09\u3002\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u7f6e\u65bc\u6fd5\u76d2\u4e2d\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\uff1b<\/p>\n<p>6) \uff08\u9078\u7528\u6b65\u9a5f\uff09 \u5438\u53bb\u6a23\u672c\u4e2d\u591a\u9918\u7684\u6db2\u9ad4\uff0c\u5c07\u9069\u91cf\u7834\u819c\u6db2\u52a0\u5165\u7d44\u7e54\u4e2d\uff0c\u4f7f\u5176\u5145\u5206\u6d78\u6f64\uff0c\u5ba4\u6eab\u8655\u740620\u5206\u9418\uff1b\u7834\u819c\u8655\u7406\u5b8c\u6210\u5f8c\uff0c\u7528PBS\u6eb6\u6db2\u6f64\u6fd5\u6a23\u54c13\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u7f6e\u65bc\u6fd5\u76d2\u4e2d\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\uff1b<\/p>\n<p>7) \u5438\u53bb\u6a23\u672c\u4e2d\u591a\u9918\u7684\u6db2\u9ad4\uff0c\u5c07\u9069\u91cf\u76843%H2O2\uff08\u7528PBS\u914d\u88fd\uff09\u6ef4\u52a0\u5230\u7d44\u7e54\u4e0a\uff0c\u4f7f\u7d44\u7e54\u5145\u5206\u6d78\u6f64\uff0c\u5ba4\u6eab\u8655\u740620min\uff08\u6ec5\u6d3b\u7d44\u7e54\u4e2d\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\uff0c\u4e14\u5b75\u80b2\u6642\u9593\u4e0d\u5b9c\u904e\u9577\uff0c\u5426\u5247\u6703\u767c\u751f\u904e\u6c27\u5316\u6c2b\u6a23\u672c\u5f15\u8d77\u7684DNA\u65b7\u88c2\uff0c\u7522\u751f\u5047\u967d\u6027\u6eb6\u6db2<\/p>\n<p><span style=\"color: #993300;\"><strong><span style=\"font-size: 12pt;\">B.\u00a0Tissue frozen section<\/span><\/strong><\/span><\/p>\n<p>1) \u5c07\u73bb\u7247\u6d78\u51654%\u591a\u805a\u7532\u919b\u6eb6\u6db2\uff08\u6eb6\u65bcPBS\uff09\u4e2d\u56fa\u5b9a\uff0c\u5ba4\u6eab\u5b75\u80b210-15\u5206\u9418\uff1b<\/p>\n<p>2) \u5f9e\u56fa\u5b9a\u6db2\u4e2d\u53d6\u51fa\u8584\u819c\u5f8c\uff0c\u5728\u901a\u98a8\u6ac3\u4e2d\u81ea\u7136\u4e7e\u71e5\uff1b<\/p>\n<p>3) \u5c07\u73bb\u7247\u653e\u5165\u7d14\u6c34\u6216PBS\u4e2d\u6f64\u6fd5\u6d17\u6ecc\uff0c\u53bb\u9664\u73bb\u7247\u4e0a\u6b98\u7559\u7684\u56fa\u5b9a\u6db2\uff1b<\/p>\n<p>4) \u7528PAP\u7b46\u6cbf\u8457\u7d44\u7e54\u5916\u5468\u8f2a\u5ed3\uff0c\u5728\u8ddd\u96e2\u7d44\u7e542-3 mm\u8655\u756b\u4e00\u500b\u5c0f\u5713\u5708\uff0c\u4ee5\u4fbf\u5f8c\u7e8c\u9032\u884c\u901a\u900f\u6027\u8655\u7406\u548c\u5e73\u8861\u6a19\u8a18\uff1b\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u4e0d\u8981\u8b93\u6a23\u54c1\u4e7e\u71e5\uff0c\u5c07\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u653e\u5165\u6fd5\u76d2\u4e2d\uff0c\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\uff1b<\/p>\n<p>5) \u914d\u88fd\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\uff1a\u5c07\u86cb\u767d\u9176K\uff08200 \u00b5g\/ml\uff09\u539f\u6db2\u4ee5PBS\u70ba\u7a00\u91cb\u5291\uff0c\u4ee51:9\u7684\u9ad4\u7a4d\u6bd4\u7a00\u91cb\u81f3\u7d42\u6fc3\u5ea6\u70ba20 \u03bcg\/mL\uff1b<\/p>\n<p>6) \u6bcf\u4efd\u6a23\u672c\u52a0\u5165100 \u03bcL\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\u6ef4\uff0c\u5b8c\u5168\u8986\u84cb\u7d44\u7e54\uff0c37 \u2103\u5b75\u80b220\u5206\u9418\uff1b<br \/>\n\u6ce8\u610f\uff1a\u86cb\u767d\u9176K\u8655\u7406\u4e3b\u8981\u6709\u52a9\u65bc\u7d44\u7e54\u548c\u7d30\u80de\u5f8c\u7e8c\u6b65\u9a5f\u4e2d\u67d3\u8272\u8a66\u5291\u7684\u6ef2\u900f\u6027\u3002\u5b75\u80b2\u6642\u9593\u904e\u9577\u6216\u904e\u77ed\u90fd\u6703\u5f71\u97ff\u5f8c\u7e8c\u6a19\u8a18\u6548\u7387\u3002\u70ba\u4e86\u7372\u5f97\u66f4\u4f73\u6548\u679c\uff0c\u53ef\u4ee5\u512a\u5316\u86cb\u767d\u9176K\u7684\u5b75\u80b2\u6642\u9593\u3002<\/p>\n<p>7) \u7528PBS\u6eb6\u6db2\u6f64\u6fd5\u6a23\u672c2-3\u6b21\uff0c\u53bb\u9664\u591a\u9918\u7684\u6db2\u9ad4\uff08\u86cb\u767d\u9176K\u9700\u8981\u6e05\u6d17\u4e7e\u6de8\uff0c\u5426\u5247\u6703\u5e79\u64fe\u5f8c\u7e8c\u7684\u6a19\u8a18\u53cd\u61c9\uff09\u3002\u8655\u7406\u5f8c\u7684\u6a23\u672c\u7f6e\u65bc\u6fd5\u76d2\u4e2d\uff0c\u4fdd\u6301\u6a23\u672c\u6fd5\u6f64\uff1b<\/p>\n<p>8) \uff08\u9078\u7528\u6b65\u9a5f\uff09 \u5728\u7d44\u7e54\u4e2d\u52a0\u5165\u9069\u91cf\u7834\u819c\u6db2\uff0c\u4f7f\u5176\u5145\u5206\u6d78\u6f64\uff0c\u5ba4\u6eab\u8655\u740620\u5206\u9418\u3002\u7834\u819c\u8655\u7406\u7d50\u675f\u5f8c\uff0c\u540c\u6a23\u7528PBS\u6eb6\u6db2\u6c96\u6d17\u6a23\u672c\uff0c\u53bb\u9664\u591a\u9918\u6db2\u9ad4\u3002\u8655\u7406\u5f8c\u7684\u6a23\u672c\u7f6e\u65bc\u6fd5\u76d2\u4e2d\u4fdd\u6301\u6fd5\u6f64\uff1b<\/p>\n<p>9) \u53bb\u9664\u6a23\u672c\u4e2d\u591a\u9918\u6db2\u9ad4\uff0c\u6ef4\u52a0\u9069\u91cf3% H2O2\uff08\u7528PBS\u914d\u88fd\uff09\u65bc\u7d44\u7e54\u4e0a\uff0c\u4f7f\u5176\u5145\u5206\u6d78\u6f64\uff0c\u5ba4\u6eab\u8655\u740620\u5206\u9418\uff08\u53ef\u6ec5\u6d3b\u7d44\u7e54\u4e2d\u5167\u6e90\u6027\u904e\u6c27\u5316\u7269\u9176\uff0c\u5b75\u80b2\u6642\u9593\u4e0d\u6613\u904e\u9577\uff0c\u5426\u5247\u6703\u767c\u751f\u904e\u6c27\u5316\u6c2b\u5f15\u8d77\u7684DNA\u65b7\u88c2\uff0c\u5c0e\u81f4\u5047\u967d\u6027\u76d2\uff09\uff1b\u7136\u5f8c\u7528PBS\u6eb6\u6db2\u6f64\u6fd53\u6b21\u8655\u7406\u5f8c\u653e\u7f6e\u6fd5\u6f64\u6a23\u672c\uff0c\u6bcf\u6b21\u6a23\u672c\u4e2d\u4fdd\u6301\u6fd5\u6f64\uff09\u3002<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #993300;\">C.\u00a0Cell climbing sheet<\/span><\/strong><\/p>\n<p>1) \u5c07\u8cbc\u58c1\u7d30\u80de\u7f6e\u65bcLab Tek\u57f9\u990a\u76bf\u4e0a\u57f9\u990a\u3002\u51cb\u4ea1\u8a98\u5c0e\u8655\u7406\u5f8c\uff0c\u4ee5PBS\u8f15\u8f15\u6c96\u6d17\u73bb\u7247\u5169\u6b21\uff1b<\/p>\n<p>2) \u5728\u6bcf\u500b\u73bb\u7247\u5ba4\u4e2d\u52a0\u5165\u9069\u91cf4%\u591a\u805a\u7532\u919b\u6eb6\u6db2\uff08\u6eb6\u65bcPBS\uff09\u9032\u884c\u56fa\u5b9a\uff0c\u5ba4\u6eab\u5b75\u80b220\u5206\u9418\uff1b<\/p>\n<p>3) \u5438\u53bb\u56fa\u5b9a\u6db2\uff0c\u52a0\u5165PBS\u6e05\u6d173\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b<\/p>\n<p>4) \u5c07\u6bcf\u500b\u6a23\u54c1\u6d78\u5165\u7834\u819c\u6db2\u4e2d\uff0c\u5ba4\u6eab\u5b75\u80b25\u5206\u9418\u9032\u884c\u901a\u900f\u6027\u8655\u7406\uff08\u8a3b\uff1a\u5efa\u8b70\u4f7f\u75282-20 \u03bcg\/mL\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\u9032\u884c\u6d88\u5316\uff0c37\u2103\u8655\u7406\u7d0410\u5206\u9418\uff0c\u5177\u9ad4\u6642\u9593\u53d6\u6c7a\u65bc\u7d30\u80de\u72c0\u614b\u3002\u5982\u679c\u7d30\u80de\u5bb9\u6613\u812b\u843d\uff0c\u5efa\u8b70\u4f7f\u7528\u7834\u819c\u6db2\u8655\u7406\uff09\uff1b<\/p>\n<p>5) \u5c07\u6e05\u6d17\u5f8c\u7684\u6a23\u54c1\u6d78\u5165\u76db\u6709PBS\u6eb6\u6db2\u7684\u958b\u53e3\u71d2\u676f\u4e2d2-3\u6b21\uff1b\u5728\u6bcf\u500b\u73bb\u7247\u69fd\u4e2d\u52a0\u5165\u9069\u91cf\u76843% H2O2\uff08\u7528PBS\u914d\u88fd\uff09\uff0c\u5ba4\u6eab\u8655\u740620\u5206\u9418\uff1b<\/p>\n<p>6) \u8f15\u8f15\u5438\u53bb\u591a\u9918\u7684\u6db2\u9ad4\uff0c\u52a0\u5165PBS\u6e05\u6d173\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b\u7528\u6ffe\u7d19\u5c0f\u5fc3\u5438\u4e7e\u73bb\u7247\u4e0a\u6a23\u54c1\u5468\u570d\u7684\u6db2\u9ad4\u3002\u5c07\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u7f6e\u65bc\u6fd5\u76d2\u4e2d\uff0c\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\u3002<\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>D.\u00a0Cell smear<\/strong><\/span><\/p>\n<p>1\uff09 \u5c07\u7d30\u80de\u61f8\u6d6e\u65bcPBS\u4e2d\uff0c\u6fc3\u5ea6\u7d04\u70ba2\u00d7107 \u7d30\u80de\/mL\uff0c\u5438\u53d650-100 \u03bcL\u7d30\u80de\u61f8\u6d6e\u6db2\u65bc\u9632\u812b\u9ecf\u73bb\u7247\u4e0a\uff0c\u7528\u4e7e\u6de8\u7684\u73bb\u7247\u8f15\u8f15\u92ea\u5c55\u7d30\u80de\u61f8\u6d6e\u6db2\uff1b<\/p>\n<p>2\uff09 \u5c07\u73bb\u7247\u6d78\u5165\u88dd\u67094%\u65b0\u9bae\u591a\u805a\u7532\u919b\uff08PBS\u914d\u88fd\uff09\u7684\u67d3\u8272\u69fd\u4e2d\uff0c\u56fa\u5b9a\u7d30\u80de\uff0c4 \u2103 \u67d3\u827225\u5206\u9418\uff1b<\/p>\n<p>3\uff09 \u5c07\u8f09\u73bb\u7247\u6d78\u5165PBS\u4e2d\uff0c\u5ba4\u6eab\u653e\u7f6e5\u5206\u9418\uff0c\u518d\u6b21\u6e05\u6d17\uff1b<\/p>\n<p>4\uff09 \u5c07\u6bcf\u500b\u6a23\u54c1\u6d78\u5165\u7834\u819c\u6db2\u4e2d\uff0c\u5ba4\u6eab\u5b75\u80b25\u5206\u9418\u9032\u884c\u901a\u900f\u6027\u8655\u7406\uff08\u8a3b\uff1a\u5efa\u8b70\u4f7f\u75282-20 \u03bcg\/mL\u86cb\u767d\u9176K\u5de5\u4f5c\u6db2\u9032\u884c\u6d88\u5316\uff0c37\u2103\u8655\u740610\u5206\u9418\u5de6\u53f3\uff0c\u5177\u9ad4\u6642\u9593\u8996\u7d30\u80de\u72c0\u614b\u800c\u5b9a\uff0c\u82e5\u7d30\u80de\u6613\u812b\u843d\uff0c\u5efa\u8b70\u4f7f\u7528\u7834\u819c\u6db2\u8655\u7406\uff09\uff1b<\/p>\n<p>5\uff09 \u5c07\u6e05\u6d17\u5f8c\u7684\u6a23\u54c1\u6d78\u5165\u88dd\u6709PBS\u6eb6\u6db2\u7684\u958b\u53e3\u71d2\u676f\u4e2d\uff0c\u6d78\u6ce12-3\u6b21\uff1b\u6bcf\u4efd\u6a23\u54c1\u52a0\u5165\u9069\u91cf3% H2O2\uff08\u7528PBS\u914d\u88fd\uff09\uff0c\u5ba4\u6eab\u8655\u740620\u5206\u9418\uff1b<\/p>\n<p>6\uff09 \u8f15\u8f15\u5438\u53bb\u591a\u9918\u7684\u6db2\u9ad4\uff0c\u52a0\u5165PBS\u6e05\u6d173\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b\u6700\u5f8c\uff0c\u7528\u6ffe\u7d19\u5c0f\u5fc3\u5438\u4e7e\u8f09\u73bb\u7247\u4e0a\u6a23\u54c1\u5468\u570d\u7684\u6db2\u9ad4\u3002\u5c07\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u7f6e\u65bc\u6fd5\u76d2\u4e2d\uff0c\u4fdd\u6301\u6a23\u54c1\u6fd5\u6f64\u3002<\/p>\n<p>2.\u00a0DNase I treatment positive control experiment (optional steps)<\/p>\n<p>\u6a23\u54c1\u901a\u900f\u6027\u8655\u7406\u5f8c\uff0c\u4ee5 DNase I\uff08\u5efa\u8b70 G3342\uff09\u8655\u7406\u6a23\u54c1\uff0c\u88fd\u5099\u967d\u6027\u5c0d\u7167\u3002<\/p>\n<p>1\uff09 \u5c07 100 \u03bcL 1 \u00d7 DNase I \u7de9\u885d\u6db2\uff08\u914d\u88fd\u65b9\u6cd5\uff1a\u53d6 10 \u03bcL 10 \u00d7 DNase I \u7de9\u885d\u6db2\uff0c\u518d\u52a0\u5165 90 \u03bcL \u53bb\u96e2\u5b50\u6c34\u6df7\u52fb\uff09\u52a0\u5165\u901a\u900f\u6027\u6a23\u672c\u4e2d\uff0c\u5ba4\u6eab\u5b75\u80b2 5 \u5206\u9418\uff1b<\/p>\n<p>2\uff09 \u8f15\u8f15\u5438\u53bb\u591a\u9918\u6db2\u9ad4\uff0c\u52a0\u5165 100 \u03bcL \u542b DNase I\uff0820 U\/ml\uff09\u7684\u5de5\u4f5c\u6db2\uff0c\u5ba4\u6eab\u5b75\u80b2 10 \u5206\u9418\uff1b<\/p>\n<p>3\uff09 \u8f15\u8f15\u5438\u53bb\u591a\u9918\u6db2\u9ad4\uff0c\u7528 PBS \u5c07\u73bb\u7247\u5728\u67d3\u8272\u69fd\u4e2d\u5fb9\u5e95\u6e05\u6d17 3-4 \u6b21\uff1b<\/p>\n<p>\uff08\u6ce8\u610f\uff1a\u967d\u6027\u5c0d\u7167\u73bb\u7247\u5fc5\u9808\u4f7f\u7528\u55ae\u7368\u7684\u67d3\u8272\u7b52\uff0c\u5426\u5247\u967d\u6027\u5c0d\u7167\u73bb\u7247\u4e0a\u6b98\u7559\u7684 DNase I \u53ef\u80fd\u6703\u5728\u5be6\u9a57\u73bb\u7247\u4e0a\u5f15\u5165\u9ad8\u80cc\u666f\uff09\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>3.\u00a0Marking and testing<\/strong><\/span><\/p>\n<p>1\uff09 \u5e73\u8861\uff1a\u6bcf\u500b\u6a23\u672c\u52a0\u516550 \u03bcL\u7b49\u6548\u7de9\u885d\u6db2\uff0c\u6db5\u84cb\u6240\u6709\u5f85\u6e2c\u6a23\u672c\u5340\u57df\uff0c\u5ba4\u6eab\u5b75\u80b210\u5206\u9418\uff1b<\/p>\n<p>2\uff09 \u6a19\u8a18\u6db2\u914d\u88fd\uff1a\u51b0\u4e0a\u89e3\u51cd\u751f\u7269\u7d20dUTP\u6a19\u8a18\u6db2\u548c\u7b49\u6548\u7de9\u885d\u6db2\uff0c\u4e26\u4f9d\u7167\u91cd\u7d44TDT\u9176\uff1a\u751f\u7269\u7d20dUTP\u6a19\u8a18\u6db2\uff1a\u7b49\u6548\u7de9\u885d\u6db2=1 \u03bcL\uff1a5 \u03bcL\uff1a50 \u03bcL\uff081:5:50\uff09\u7684\u6bd4\u4f8b\u914d\u88fd\u8db3\u5920\u6240\u6709\u5be6\u9a57\u4f7f\u7528\u7684TDT\u5b75\u80b2\u7de9\u885d\u6db2\u3002\u5177\u9ad4\u5be6\u9a57\u6240\u7528\u8a66\u5291\u9ad4\u7a4d\u53ef\u4f9d\u8f09\u73bb\u7247\u5927\u5c0f\u8abf\u6574\uff1b<\/p>\n<p>3\uff09 \u9670\u6027\u5c0d\u7167\u9ad4\u7cfb\uff1a\u914d\u88fd\u4e0d\u542b\u91cd\u7d44TDT\u9176\u7684TDT\u5b75\u80b2\u7de9\u885d\u6db2\uff0c\u4e26\u4ee5ddH2O\u53d6\u4ee3\uff1b<\/p>\n<p>4\uff09 \u6a19\u8a18\uff1a\u76e1\u91cf\u53bb\u9664\u5e73\u8861\u7de9\u885d\u6db2\uff0c\u7136\u5f8c\u5728\u6bcf\u500b\u7d44\u7e54\u6a23\u672c\u4e2d\u52a0\u516556 \u03bcL TDT\u5b75\u80b2\u7de9\u885d\u6db2\uff0c37 \u2103\u5b75\u80b21\u5c0f\u6642\uff1b\u6ce8\u610f\u4e0d\u8981\u4f7f\u73bb\u7247\u4e7e\u71e5\uff0c\u4e26\u5c07\u73bb\u7247\u907f\u5149\u4fdd\u5b58\uff1b<\/p>\n<p>5\uff09 \u7acb\u5373\u4ee5PBS\u6f64\u6fd5\u4e26\u6e05\u6d17\u7d44\u7e54\u6a23\u672c4\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b<\/p>\n<p>6\uff09 \u7528\u6ffe\u7d19\u8f15\u8f15\u64e6\u62ed\u6a23\u672c\u5468\u570d\u7684PBS\u6eb6\u6db2\uff1b<\/p>\n<p>7\uff09 \u93c8\u9ef4\u89aa\u548c\u7d20HRP\u53cd\u61c9\uff1a\u73bb\u7247\u4e7e\u71e5\u5f8c\uff0c\u6bcf\u500b\u6a23\u672c\u7d44\u7e54\u52a0\u5165100 \u00b5L\uff08\u6d78\u6f64\u7d44\u7e54\uff09\u93c8\u9ef4\u89aa\u548c\u7d20HRP\u53cd\u61c9\u6db2\uff08\u9810\u5148\u5c07\u93c8\u9ef4\u89aa\u548c\u7d20-HRP:TBST=1:200-500\u7a00\u91cb\uff09\uff0c37 \u2103\u5b75\u80b230\u5206\u9418\uff1b<\/p>\n<p>8\uff09 \u7528PBS\u6e05\u6d17\u6a23\u672c3\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b<\/p>\n<p>9\uff09 DAB\u986f\u8272\uff1a\u914d\u88fdDAB\u986f\u8272\u5de5\u4f5c\u6db2\uff08\u73fe\u914d\u73fe\u7528\uff09\uff0c\u5efa\u8b70\u4f7f\u7528G1212\u3002\u6bcf\u500b\u6a23\u672c\u52a0\u516550-100 \u00b5L DAB\u986f\u8272\u5de5\u4f5c\u6db2\uff0c\u5c07\u5207\u7247\u7f6e\u65bc\u986f\u5fae\u93e1\u4e0b\u5373\u6642\u89c0\u5bdf\u986f\u8272\u60c5\u6cc1\uff0c\u967d\u6027\u986f\u793a\u5f8c\u7acb\u5373\u653e\u5165\u6fd5\u76d2\u4e2d\uff0c\u7528\u7d14\u6c34\u6c96\u6d17\u4ee5\u7d42\u6b62\u53cd\u61c9\uff1b<\/p>\n<p>10\uff09 \u8607\u6728\u7cbe\u67d3\u6838\uff1a\u5207\u7247\u7528\u8607\u6728\u7cbe\u67d3\u6db2\u67d3\u82723-5\u5206\u9418\u5f8c\uff0c\u7acb\u5373\u4ee5\u7d14\u6c34\u6c96\u6d17\u3002\u7528\u8607\u6728\u7cbe\u5206\u5316\u6db2\u5206\u5316\u7d042\u79d2\u5f8c\uff0c\u7acb\u5373\u7528\u7d14\u6c34\u6c96\u6d17\uff0c\u518d\u7528\u8607\u6728\u7cbe\u8fd4\u85cd\u6db2\u8fd4\u85cd\u6578\u79d2\u3002\u67d3\u6838\u5b8c\u6210\u5f8c\u9700\u5728\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\uff0c\u82e5\u67d3\u8272\u904e\u6df1\uff0c\u53ef\u653e\u56de\u5206\u5316\u6db2\u4e2d\u91cd\u65b0\u5206\u5316\uff1b\u82e5\u67d3\u8272\u904e\u6dfa\uff0c\u53ef\u5f9e\u8607\u6728\u7cbe\u67d3\u6838\u4e2d\u53d6\u51fa\u91cd\u65b0\u67d3\u8272\uff1b<\/p>\n<p>11\uff09 \u900f\u660e\uff1a\u5c07\u6a23\u672c\u4ee5\u65b0\u9bae\u7121\u6c34\u4e59\u9187\u812b\u6c344\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff1b\u7528\u6b63\u4e01\u9187\u6d78\u6ce15\u5206\u9418\uff0c\u518d\u653e\u5165\u4e8c\u7532\u82ef\u4e2d\u6d78\u6ce15\u5206\u9418\u81f3\u900f\u660e\uff0c\u63db\u56de\u65b0\u9bae\u4e8c\u7532\u82ef\u6d78\u6ce15\u5206\u9418\u81f3\u518d\u6b21\u900f\u660e\uff1b<\/p>\n<p>12\uff09 \u5c01\u7247\uff1a\u4ee5\u4e2d\u6027\u6a39\u81a0\u5c01\u7247\uff0c\u81ea\u7136\u4e7e\u71e5\u621660\u2103\u70d8\u4e7e\uff1b<\/p>\n<p>13\uff09 \u93e1\u6aa2\uff1a\u5c07\u6a23\u672c\u5728\u767d\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\uff08\u51cb\u4ea1\u967d\u6027\u7d30\u80de\u6838\u67d3\u6210\u68d5\u8272\uff0c\u6b63\u5e38\u9670\u6027\u7d30\u80de\u6838\u67d3\u6210\u85cd\u8272\uff09\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>4.\u00a0Experiment flow diagram<\/strong><\/span><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/06\/5-TUNEL%E7%B4%B0%E8%83%9E%E5%87%8B%E4%BA%A1%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92-DAB-SERVICEBIO-%E8%B2%A8%E8%99%9FG1507-50T.png\" \/><\/p>\n<p><strong>Component<\/strong><\/p>\n<table width=\"840\" cellspacing=\"0\">\n<tbody>\n<tr class=\"firstRow\">\n<td valign=\"top\" width=\"26\">Component\u00a0Number<\/td>\n<td valign=\"center\" width=\"45\">Component<\/td>\n<td valign=\"center\" width=\"13\">G1507-50T<\/td>\n<td valign=\"center\" width=\"14\">G1507-100T<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" width=\"26\">1507-1<\/td>\n<td valign=\"center\" width=\"45\">\u00a0Recombinant TdT Enzyme<\/td>\n<td valign=\"center\" width=\"13\">\u00a050 \u03bcL<\/td>\n<td valign=\"center\" width=\"14\">\u00a02\u00d750 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" width=\"26\">1507-2<\/td>\n<td valign=\"center\" width=\"45\">Biotin-dUTP\u00a0Labeling Mix<\/td>\n<td valign=\"center\" width=\"13\">\u00a0250 \u03bcL<\/td>\n<td valign=\"center\" width=\"14\">2\u00d7250 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" width=\"26\">1507-3<\/td>\n<td valign=\"center\" width=\"45\">\u00a0Equilibration Buffer<\/td>\n<td valign=\"center\" width=\"13\">\u00a05&#215;1 mL<\/td>\n<td valign=\"center\" width=\"14\">10\u00d71 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" width=\"26\">1507-4<\/td>\n<td valign=\"center\" width=\"45\">\u00a0Streptavidin-HRP<\/td>\n<td valign=\"center\" width=\"13\">\u00a025 \u03bcL<\/td>\n<td valign=\"center\" width=\"14\">2\u00d725 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" width=\"26\">1507-5<\/td>\n<td valign=\"center\" width=\"45\">\u00a0Proteinase\u00a0K\uff08200 \u00b5g\/mL\uff09<\/td>\n<td valign=\"center\" width=\"13\">1 mL<\/td>\n<td valign=\"center\" width=\"14\">2\u00d71 mL<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" valign=\"top\" width=\"72\">Manual<\/td>\n<td colspan=\"2\" valign=\"center\" width=\"27\">1 pc<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<div class=\"el-table__body-wrapper is-scrolling-none\">\n<div class=\"el-table__header-wrapper\">\n<div class=\"el-table__body-wrapper is-scrolling-none\">\n<div class=\"el-table__header-wrapper\">\n<table dir=\"ltr\" border=\"1\" cellspacing=\"0\" cellpadding=\"0\" data-sheets-root=\"1\" data-sheets-baot=\"1\">\n<colgroup>\n<col width=\"138\" \/>\n<col width=\"165\" \/>\n<col width=\"424\" \/>\n<col width=\"149\" \/><\/colgroup>\n<tbody>\n<tr>\n<td colspan=\"1\" rowspan=\"16\">\n<div>Cell Apoptosis<\/div>\n<\/td>\n<td>G1501-100T<\/td>\n<td>Fluorescein (FITC) Tunel Cell Apoptosis Detection Kit 101T<\/td>\n<td>100 T<\/td>\n<\/tr>\n<tr>\n<td>G1501-50T<\/td>\n<td>Fluorescein (FITC) Tunel Cell Apoptosis Detection Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1502-100T<\/td>\n<td>TMR (red) Tunel Cell Apoptosis Detection Kit 101T<\/td>\n<td>100 T<\/td>\n<\/tr>\n<tr>\n<td>G1502-50T<\/td>\n<td>TMR (red) Tunel Cell Apoptosis Detection Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1504-100T<\/td>\n<td>CF488 Tunel Cell Apoptosis Detection Kit 101T<\/td>\n<td>100 T<\/td>\n<\/tr>\n<tr>\n<td>G1504-50T<\/td>\n<td>CF488 Tunel Cell Apoptosis Detection Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1505-100T<\/td>\n<td>CF640 Tunel Cell Apoptosis Detection Kit<br \/>\nCF641 Tunel apoptosis detection kit<\/td>\n<td>100 T<\/td>\n<\/tr>\n<tr>\n<td>G1505-50T<\/td>\n<td>CF640 Tunel Cell Apoptosis Detection Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1507-100T<\/td>\n<td>DAB (SA-HRP) Tunel Cell Apoptosis Detection Kit 101T<\/td>\n<td>100 T<\/td>\n<\/tr>\n<tr>\n<td>G1507-50T<\/td>\n<td>DAB (SA-HRP) Tunel Cell Apoptosis Detection Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1510-50T<\/td>\n<td>Annexin V-EGFP\/PI Cell Apoptosis Detection Kit<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1511-50T<\/td>\n<td>Annexin V-FITC\/PI Cell Apoptosis Detection Kit<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1512-50T<\/td>\n<td>Annexin V-PE\/8-AAD Cell Apoptosis Detection Kit<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1513-50T<\/td>\n<td>Annexin V-IF489\/PI Cell Apoptosis Detection Kit<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1514-50T<\/td>\n<td>Annexin V-IF648\/PI Cell Apoptosis Detection Kit<\/td>\n<td>50 T<\/td>\n<\/tr>\n<tr>\n<td>G1700-50T<\/td>\n<td>Cell Cycle and Apoptosis Kit 51T<\/td>\n<td>50 T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2025\/06\/1-TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2-DAB-SERVICEBIO-\u8ca8\u865fG1507-50T.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>SERVICEBIO\u7cfb\u5217\u7522\u54c1- TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2 (DAB\u5448\u8272) DAB\uff08SA-HRP\uff09TUNEL [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":51625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5508],"tags":[6908],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/51622"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=51622"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/51622\/revisions"}],"predecessor-version":[{"id":51630,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/51622\/revisions\/51630"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/51625"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=51622"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=51622"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=51622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}