{"id":50269,"date":"2024-09-04T16:58:27","date_gmt":"2024-09-04T08:58:27","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=50269"},"modified":"2025-03-25T11:04:30","modified_gmt":"2025-03-25T03:04:30","slug":"%e5%8d%b3%e7%94%a8%e5%9e%8bihc%e8%a9%a6%e5%8a%91%e7%9b%92-ready-to-use-ihc-kit-%e8%b2%a8%e8%99%9fsl-ihc100t","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=50269","title":{"rendered":"\u5373\u7528\u578bIHC\u8a66\u5291\u76d2 | Ready-to-use IHC kit \u8ca8\u865fSL-IHC100T"},"content":{"rendered":"

\u5373\u7528\u578bIHC\u8a66\u5291\u76d2 | Ready-to-use IHC kit <\/strong>\u8ca8\u865fSL-IHC100T<\/strong><\/a><\/span><\/p>\n

Ready-to-use IHC kit (Biotin free), One-Step HRP Polymer anti-Mouse and Rabbit IgG (H+L) with DAB<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong><\/p>\n

\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08Immunohistochemistry , IHC\uff09\/\u514d\u75ab\u7d30\u80de\u5316\u5b78\uff08Immunocytochemistry , ICC\uff09\u662f\u901a\u904e\u4f7f\u7528\u6a19\u8a18\u7684\u6297\u9ad4\u4f5c\u70ba\u7279\u7570\u6027\u8a66\u5291\uff0c\u901a\u904e\u6297\u539f – \u6297\u9ad4\u76f8\u4e92\u4f5c\u7528\u4f86\u5b9a\u4f4d\u7d44\u7e54\u5207\u7247\/\u7d30\u80de\u4e2d\u7684\u6297\u539f\uff0c\u6297\u539f – \u6297\u9ad4\u76f8\u4e92\u4f5c\u7528\u901a\u904e\u8af8\u5982\u87a2\u5149\u67d3\u6599(fluorescent dye) \u3001\u9176(enzyme) \u3001\u653e\u5c04\u6027\u5143\u7d20(radioactive element) \u6216\u81a0\u9ad4\u91d1\u7684\u6a19\u8a18\u7269\u7b49\u8b93\u8868\u73fe\u53ef\u8996\u5316\u3002 \u901a\u5e38\u4f7f\u7528\u5e7e\u7a2eIHC\u6280\u8853\uff0c\u4f8b\u5982\u6a19\u8a18\u7684\u751f\u7269\u7d20\u4e8c\u6297\u93c8\u9ef4\u6297\u751f\u7269\u7d20\u86cb\u767d – \u904e\u6c27\u5316\u7269\u9176 (labeled biotin secondary antibody streptavidin-peroxidase, HRP) \uff0c\u6297HRP-HRP\uff0cABC\u50ac\u5316\u7684\u4fe1\u865f\u653e\u5927\uff0cpolymer system\u805a\u5408\u7269\u7cfb\u7d71\uff08\u4e00\u6b65\u6216\u5169\u6b65\uff09\u7b49\uff0c\u4ee5\u6aa2\u6e2c\u7d44\u7e54\u548c\u7d30\u80de\u4e2d\u7684\u6297\u539f\u3002\u5373\u7528\u578bIHC\u8a66\u5291\u76d2\u63a1\u7528\u805a\u5408\u7269\u6280\u8853( polymer system)\uff0c\u53ef\u63d0\u9ad8\u9748\u654f\u5ea6\u548c\u6aa2\u6e2c\u9748\u654f\u5ea6\u3002 \u91dd\u5c0d\u6297\u5c0f\u9f20\u548c\u6297\u5154 polymer\uff0c\u4e0d\u540c\u65bc\u514d\u75ab\u751f\u7269\u7d20\/\u6297\u751f\u7269\u7d20\u86cb\u767d\u7684\u7cfb\u7d71 (biotin\/avidin free system) \u5b83\u70ba\u7528\u6236\u63d0\u4f9b\u4e86\u5feb\u901f\u4e14\u6613\u65bc\u4f7f\u7528\u7684IHC\u6aa2\u6e2c\u7cfb\u7d71\u3002<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
\u00a0<\/strong><\/td>\nProduct<\/td>\nSize<\/td>\nStorage<\/td>\nRemark<\/td>\n<\/tr>\n
1<\/td>\nCitrate buffer (0.01mol\/L, pH6.0)<\/td>\n1L<\/td>\n4\u2103<\/td>\nSolute in 1L of deionized water or distilled water<\/td>\n<\/tr>\n
2<\/td>\nPBS\u00a0buffer<\/td>\n2L<\/td>\n4\u2103<\/td>\nSolute in\u00a02L of deionized water or distilled water<\/td>\n<\/tr>\n
3<\/td>\n\n

DON-HRP-Conjugated polyase<\/p>\n

(anti mouse \/ rabbit)<\/td>\n

6mL<\/td>\n4\u2103<\/td>\nNon-frozen, 50\u03bcL\u00a0per slice<\/td>\n<\/tr>\n
4<\/td>\n30%\u00a0H2O2<\/td>\n10mL<\/td>\n4\u2103<\/td>\nDilute into 3%\u00a0, ready to use<\/td>\n<\/tr>\n
5<\/td>\nDAB\u00a0solution\u00a0I<\/td>\n0.6mL<\/td>\n-20\u2103<\/td>\nReady to use<\/td>\n<\/tr>\n
6<\/td>\nDAB\u00a0solution\u00a0II<\/td>\n0.6mL<\/td>\n-20\u2103<\/td>\nReady to use<\/td>\n<\/tr>\n
7<\/td>\nhematoxylin<\/td>\n6ml<\/td>\n4\u2103<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nSL-IHC100T<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMouse and rabbit<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nA biotin\/avidin free system that stains membrane, cytoplasmic and nuclear antigens. Immunohistochemistry (IHC) and immunocytochemistry (ICC).<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 The assay is sensitive and stable<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Citrate buffer (0.01mol\/L, pH6.0), 1L\u00a0 (4\u2103)
\n\u2022 PBS buffer, 2L (4\u2103)
\n\u2022 DON-HRP-Conjugated polyase (anti mouse \/ rabbit), 6ML\u00a0 (4\u2103)
\n\u2022 30% H2O2 , 10ML\u00a0 (4\u2103)
\n\u2022 DAB solution I, 0.6ML (-20\u2103)
\n\u2022 DAB\u00a0solution\u00a0II, 0.6ML (-20\u2103)\u2022 hematoxylin, 6ML\u00a0 (4\u2103)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u5957\u7d44\u5167\u4e0d\u5305\u542bWashing buffer |\u00a0\u00a0Retrieval Solution<\/span><\/a>\u00a0|\u00a0<\/strong><\/span> positive or negative control | primary antibody | \u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a><\/span>\u3002<\/p>\n

 <\/p>\n

1.\u70e4\u7247\u53ca\u812b\u881f\u5207\u7247\u572865\u2103\u6046\u6eab\u70e4\u7bb1\u4e2d\u70d8\u70e41h\uff1b\u5728\u4e8c\u7532\u82ef I (xylene I) \u4e2d\u6d78\u6ce115\u5206\u9418\uff0c\u7136\u5f8c\u5728\u4e8c\u7532\u82efII (xylene II) \u4e2d\u6d78\u6ce115\u5206\u9418\u3002<\/p>\n

2.\u6c34\u5316\u5c07\u812b\u881f\u5f8c\u7684\u5207\u7247\u7528100%\u9152\u7cbeI (100% alcohol I)\u3001100%\u9152\u7cbeII (100% alcohol II)\u300195%\u9152\u7cbe(95% alcohol)\u300185%\u9152\u7cbe(85% alcohol) \u548c75%\u9152\u7cbe(75% alcohol)\u6d78\u6ce1\uff0c\u4e26\u7528\u81ea\u4f86\u6c34\u6c96\u6d1710min\u3002<\/p>\n

3. \u6297\u539f\u4fee\u5fa9\u5728\u542b0.01M\u6ab8\u6aac\u9178\u9209\u7de9\u885d\u6db2 (0.01M sodium citrate buffer solution)\uff08\u53c3\u8003125\u2103\u00b7103KPa\uff09\u7684\u57f9\u990a\u57fa\u4e2d\u4fee\u5fa915min\uff0c\u81ea\u7136\u51b7\u537b<\/p>\n

4. \u5c01\u9589(blocking-up) \u5c07\u8f09\u73bb\u7247\u7f6e\u65bc3% H2O2\u4e2d\uff0c\u6fd5\u76d2\u4e2d\u53cd\u61c910min\uff0c\u6d88\u9664\u5167\u6e90\u904e\u6c27\u5316\u7269\u9176(endogenous peroxidase activity)\u6d3b\u6027\u3002<\/p>\n

5. \u5bc6\u5c01 \u5c07\u8f09\u73bb\u7247\u653e\u51655% BSA\u4e2d\uff0c\u5728\u6fd5\u76d2\u4e2d\u53cd\u61c91 30min\uff0c\u518d\u7528PBS\u6eb6\u6db2\u6e05\u6d173min*3\u6b21\u3002<\/p>\n

6. \u5b75\u80b2\u4e00\u6297\u52a0\u5165\u4e00\u6297\uff08\u9078\u64c7\u6700\u4f73\u7a00\u91cb\u6bd4\uff09\u53cd\u61c9\uff0c\u5728\u6fd5\u76d2\u4e2d\u4ee54\u2103\u53cd\u61c9\u904e\u591c\uff0c\u4ee5PBS\u6eb6\u6db2\u6d17\u6ecc3min*3\u6b21\u3002<\/p>\n

7. \u5b75\u80b2\u4e8c\u6297 \u9664\u53bb PBS \u6eb6\u6db2\uff0c\u52a0\u5165 50 \u00b5L DON-HRP-Conjuated\uff0c\u5c07\u6fd5\u76d2\u5b75\u80b2\uff0c\u5ba4\u6eab\u653e\u7f6e 20min-30min\u3002 PBS \u6eb6\u6db2\u6d17\u6ecc 3min*3 \u6b21<\/p>\n

8. \u986f\u8272 DAB\u67d3\u8272\uff08DAB\u6eb6\u6db2I\u548cDAB\u6eb6\u6db2II\u63091:1\u6df7\u5408\uff09\uff0c\u7576\u5207\u7247\u8b8a\u8272\u6642\uff0c\u7acb\u5373\u7528\u81ea\u4f86\u6c34\u6c96\u6d17\u67d3\u8272\u6db2\u3002<\/p>\n

9.\u8607\u6728\u7cbe\u67d3\u8272\u8607\u6728\u7cbe\u518d\u67d33min\uff0c1%\u9e7d\u9178\u9152\u7cbe\u5206\u5316\uff0c\u93e1\u6aa2\u63a7\u5236\u67d3\u8272\u7a0b\u5ea6\u3002\u7528\u81ea\u4f86\u6c34\u6c96\u6d1710\u5206\u9418\u3002<\/p>\n

10. \u812b\u6c34 \u5c07\u6a23\u672c\u6d78\u6ce1\u572875%\u300185%\u300195%\u3001100%\u9152\u7cbeI\u3001100%\u9152\u7cbeII\u4e2d5\u5206\u9418\u3002<\/p>\n

11.\u5c07\u900f\u660e\u548c\u5bc6\u5c01\u73bb\u7247\u7f6e\u65bc\u4e8c\u7532\u82ef\u4e2d3min*2\u6b21\uff0c\u900f\u660e\u4e2d\u6027\u81a0\u5bc6\u5c01\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5373\u7528\u578bIHC\u8a66\u5291\u76d2 | Ready-to-use IHC kit \u8ca8\u865fSL-IHC100T Ready-to- […]<\/p>\n","protected":false},"author":3,"featured_media":50270,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5551],"tags":[6779],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50269"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=50269"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50269\/revisions"}],"predecessor-version":[{"id":50882,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/50269\/revisions\/50882"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/50270"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=50269"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=50269"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=50269"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}