{"id":49730,"date":"2024-06-28T16:51:47","date_gmt":"2024-06-28T08:51:47","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49730"},"modified":"2025-11-11T13:33:36","modified_gmt":"2025-11-11T05:33:36","slug":"svog-cell-line-abmgood-%e8%b2%a8%e8%99%9ft9144","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49730","title":{"rendered":"SVOG Cell Line | ABMGOOD (\u8ca8\u865fT9144)"},"content":{"rendered":"

A<\/strong>bmgood\u4e0d\u673d<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)-\u6c38\u751f\u5316SVOG\u7d30\u80de\u682a<\/strong><\/span><\/p>\n

SVOG Cell Line (\u8ca8\u865fT9144)<\/span><\/a><\/span><\/strong><\/p>\n

SVOG Cell Line\u7279\u6027<\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u578b\u614b: Adherent, polygonal\u3002 \u4f86\u6e90Ovary\u3002\u89e3\u51cd\u5f8c\uff0c\u7d30\u80de\u9700\u89814-5\u5929\u624d\u80fd\u6062\u5fa9\u57f9\u990a<\/span>\u3002 \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a><\/span>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrow II (\u8ca8\u865f<\/u>TM002)+ 10% FBS + 1% Penicillin\/Streptomycin Solution (G255) \u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002<\/p>\n

\u00a0\"\"<\/div>\n
\n
Specifications<\/span><\/strong><\/span><\/div>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT9144<\/span><\/td>\n<\/tr>\n
Name<\/td>\nSVOG Cell Line<\/td>\n<\/tr>\n
Organism<\/td>\nHuman (H. sapiens)<\/td>\n<\/tr>\n
Tissue<\/td>\nOvary<\/td>\n<\/tr>\n
Donor History<\/td>\nFemale<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent, polygonal<\/td>\n<\/tr>\n
Cell Type<\/td>\nImmortalized Cells<\/td>\n<\/tr>\n
Storage Condition<\/td>\nVapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n
Shipping Conditions<\/td>\nShip with dry ice.<\/td>\n<\/tr>\n
Product Format<\/td>\nFrozen<\/td>\n<\/tr>\n
Intended Use<\/td>\nThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n
BioSafety<\/td>\nII<\/td>\n<\/tr>\n
Certificate of Analysis<\/td>\nFor batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n
Growth Conditions<\/td>\nUse of PriCoat\u2122 T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u00b0C, 5% CO\u2082<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/a><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n

\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u00a0(immortalized reagenet)\u00a0Ready-to-use<\/span><\/strong><\/span><\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002<\/p>\n

\"cell-biology-tab-final<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Decomplement<\/strong>\u52a0\u71b1\u975e\u6d3b\u5316FBS\uff1a<\/strong><\/span><\/p>\n

1.\u8b93FBS\u74f6\u57284\u00b0C\u4e0b\u5b8c\u5168\u89e3\u51cd\u904e\u591c\u3002<\/p>\n

2.\u5c07FBS\u74f6\u7f6e\u65bc56\u00b0C\u7684\u6c34\u6d74\u4e2d30\u5206\u9418\u3002<\/p>\n

Decomplemented FBS\u53ef\u4ee5\u5728-20\u00b0C\u4e0b\u9577\u671f\u4fdd\u5b58\u3002\u907f\u514d\u983b\u7e41\u7684\u51cd\u878d\u5faa\u74b0\u3002\u6bcf2-3\u5929\u66f4\u63db\u4e00\u6b21\u5b8c\u6574\u7684\u5a92\u9ad4\u3002\u4e0d\u8981\u8b93\u4ecb\u8cea\u984f\u8272\u8b8a\u70ba\u9ec3\u8272\u3002<\/p>\n

Thawing<\/strong>\u7d30\u80de\u89e3\u51cd\u65b9\u6cd5\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u572837\u2103\u6c34\u6d74\u4e2d\u89e3\u51cd\u5c0f\u74f6\uff0c\u76f4\u5230\u53ea\u6709\u4e00\u5c0f\u584a\u51b0\u584a\u3002(DMSO\u5c0d\u7d30\u80de\u6709\u6bd2\u6027\uff0cover-thaw\u904e\u5ea6\u89e3\u51cd\u4e0b\u53ef\u80fd\u9020Stress)<\/span><\/p>\n

2.\u5c07\u7d30\u80de\u8f49\u79fb\u5230\u542b\u67095ml pre-warmed complete media \u4e4b15ml\u7ba1\u4e2d\u3002<\/p>\n

3.\u5c07\u7d30\u80de\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

4.\u5c0f\u5fc3\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u6253\u64fe\u7d30\u80de\u6c89\u6fb1\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u57281ml\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

5.\u5c07\u7d30\u80de\u57f9\u990a\u572850,000 – 100,000 cells\/cm2<\/sup>\u3002\u00a0cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5<\/p>\n

\"\"<\/p>\n

DMSO\u5728\u7d30\u80de\u51cd\u5b58\u4e2d\u7684\u61c9\u7528<\/span><\/strong><\/p>\n

\u4e8c\u7532\u57fa\u4e9e\u78b8\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u6ef2\u900f\u578b\u7d30\u80de\u4fdd\u8b77\u5291\u3002\u5728\u6df1\u4f4e\u6eab\uff08\u96f6\u4e0b200\u5ea6\uff09\u4fdd\u5b58\u7d30\u80de\u6642\u51cd\u5b58\u904e\u7a0b\u4e2d\uff0c\u70ba\u9632\u6b62\u7d30\u80de\u5167\u6db2\u51b0\u6676\u5f62\u6210\u3001\u6ef2\u900f\u58d3\u6539\u8b8a\u3001\u7d30\u80de\u7d50\u69cb\u7d0a\u4e82\u7b49\u5c0e\u81f4\u7684\u640d\u50b7\uff0c\u6709\u5fc5\u8981\u4f7f\u7528\u542b\u6709DMSO\u51b7\u51cd\u4fdd\u8b77\u5291\u3002DMSO\u80fd\u5920\u5feb\u901f\u7a7f\u900f\u7d30\u80de\u819c\u9032\u5165\u7d30\u80de\u4e2d\uff0c\u964d\u4f4e\u51b0\u9ede\u3001\u5ef6\u7de9\u51cd\u5b58\u904e\u7a0b\uff0c\u540c\u6642\u63d0\u9ad8\u7d30\u80de\u5167\u96e2\u5b50\u6fc3\u5ea6\uff0c\u6e1b\u5c11\u7d30\u80de\u5167\u51b0\u6676\u7684\u5f62\u6210\uff0c\u5f9e\u800c\u6e1b\u5c11\u7d30\u80de\u640d\u50b7\u3002\u6df1\u4f4e\u6eab\u6642\u4e8c\u7532\u57fa\u4e9e\u78b8\u7684\u7d30\u80de\u6bd2\u6027\u53d7\u5230\u6291\u5236\u3002\u5fa9\u8607\u6642\u52d5\u4f5c\u8981\u5feb\uff0c\u5118\u5feb\u6d17\u6389\u4e8c\u7532\u57fa\u4e9e\u78b8\uff0c\u5426\u5247\u6703\u9020\u6210\u5c0d\u7d30\u80de\u56b4\u91cd\u7684\u6bd2\u6027\u3002\u4e8c\u7532\u57fa\u4e9e\u78b8\uff08DMSO\uff09\u662f\u76ee\u524d\u6700\u597d\u7684\u7d30\u80de\u51cd\u5b58\u4fdd\u8b77\u5291\uff0c\u4f46\u4e5f\u662f\u4e00\u7a2e\u4ee5\u7d30\u80de\u6bd2\u6027\u5f88\u5927\u7684\u5316\u5b78\u8a66\u9a57\u5291\u3002\u7814\u7a76\u7d50\u679c\u8868\u660e\uff0c\u57f9\u990a\u6db2\u4e2dDMSO\u6fc3\u5ea6\u70ba10%\u6642\uff0c\u7d30\u80de\u751f\u9577\u6291\u5236\u7387\u8fd1100%\uff1b1\u2030\u6fc3\u5ea6\u6642\u6291\u5236\u7387\u70ba35%\uff0c\u5373\u4f7f\u662f0.04\u2030\u7684\u6fc3\u5ea6\uff0cDMSO\u5c0d\u7d30\u80de\u7684\u751f\u9577\u4e5f\u6709\u4e0d\u5229\u7684\u5f71\u97ff\u3002<\/p>\n

Subculture<\/strong>\u7d30\u80de\u7e7c\u4ee3\u65b9\u6cd5:<\/strong>\u00a0(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)<\/strong>\u00a0\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u7576\u7d30\u80de70-90\uff05 confluency\u6642\uff0c\u5efa\u8b70\u7e7c\u4ee3\u57f9\u990a\u3002<\/p>\n

2. Aspirate old media\u3002\u61f8\u6d6e\u6db2\u4e2d\u7684\u4e00\u4e9b\u7d30\u80de\u4ecd\u7136\u662f\u6d3b\u7d30\u80de\u3002\u6216\u8005\uff0c\u4e5f\u53ef\u4ee5\u6536\u96c6\u542b\u6709\u61f8\u6d6e\u7d30\u80de\u7684\u4e0a\u6e05\u6db2\u4e26\u96e2\u5fc3\uff08Skip to Step 6 in protocol\uff09\u3002<\/p>\n

3.\u52a0\u51651\uff1a1\u6bd4\u4f8b\u76841X\u7121\u83ccPBS\u548c0.25\uff05\u80f0\u86cb\u767d\u9176-EDTA\uff08TM051\uff09\u3002<\/p>\n

4.\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u7d30\u80de3-5\u5206\u9418\u4e26\u652a\u62cc\u57f9\u990a\u5bb9\u5668\u76f4\u81f390\uff05\u7684\u7d30\u80de\u812b\u843d\u3002<\/p>\n

5. \u4e2d\u548c\u80f0\u86cb\u767d\u9176:\u6dfb\u52a0\u7b49\u91cf\u4e4btrypsin + PBS\u3002<\/p>\n

6.\u6536\u96c6\u7d30\u80de\u4e26\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

7.\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

8.\u5efa\u8b70split ratio\u6bd4\u4e0d\u8d85\u904e1\uff1a3\u3002<\/p>\n

Freezing<\/strong>\u7d30\u80de\u51cd\u7d50\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/a><\/strong><\/span><\/span><\/p>\n

Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.<\/p>\n

\u5132\u5b58\u6eab\u5ea6\uff1a\u6db2\u6c2e\u6c23\u76f8\u3002\u91cd\u8981\u63d0\u793a\uff1a\u6b64\u7d30\u80de\u4fc2\u5c0dFBS\u6279\u6b21\u975e\u5e38\u654f\u611f\u3002\u6211\u5011\u5f37\u70c8\u5efa\u8b70\u6700\u7d42\u7528\u6236\u4f7f\u7528\u5f9ePAN biotech\u6216Nucleus Biologics \u4e4bFBS\u3002<\/p>\n

\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1Lentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus\u00a0<\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus<\/u>\u75c5\u6bd2RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat No<\/strong><\/td>\nProduct Name<\/strong><\/td>\n<\/tr>\n
T0016<\/td>\nImmortalized Human Cord Blood Mesenchymal Stromal Cells (cbMSC-hTERT)<\/td>\n<\/tr>\n
T0017<\/td>\nImmortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP)<\/td>\n<\/tr>\n
T0533<\/td>\nImmortalized Human Endometrial Stromal Cells (HESC)<\/td>\n<\/tr>\n
T0697<\/td>\nImmortalized Human Cranial Suture Progenitor Cells (iSuPs)<\/td>\n<\/tr>\n
T0723<\/td>\nImmortalized Human Foreskin Keratinocytes (HFK-16E6E7)<\/td>\n<\/tr>\n
T0727<\/td>\nImmortalized Human Prostate Stromal Cells (PM151T) – hTERT<\/td>\n<\/tr>\n
T0752<\/td>\nImmortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5)<\/td>\n<\/tr>\n
T0764<\/td>\nImmortalized Human Endometriotic Cell Line (12Z)<\/td>\n<\/tr>\n
T0841<\/td>\nImmortalized Human Dental Pulp Stem Cells (TP-023)<\/td>\n<\/tr>\n
T0842<\/td>\nImmortalized Human Dental Pulp Stem Cells (TP-037)<\/td>\n<\/tr>\n
T1013<\/td>\nImmortalized Human Diploid Fibroblasts – hTERT<\/td>\n<\/tr>\n
T1015<\/td>\nImmortalized Human Lung Fibroblast – hTERT<\/td>\n<\/tr>\n
T9435<\/td>\nImmortalized Human Cancer Associated Fibroblast Cells – SV40<\/td>\n<\/tr>\n
T3000<\/td>\nAcan CRISPR Knockout RCS Cell Line (Rat)<\/td>\n<\/tr>\n
T3002<\/td>\nAquaporin-4 Stable 293 Cell Line<\/td>\n<\/tr>\n
T3005<\/td>\nMuSK Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3006<\/td>\nLuciferase Stable PC3 Cell Line<\/td>\n<\/tr>\n
T3008<\/td>\nLuciferase (Luc2) Stable TRAMP Cell Line<\/td>\n<\/tr>\n
T3009<\/td>\nLuciferase Stable U87MG\/\u0394EGFR Cell Line<\/td>\n<\/tr>\n
T3013<\/td>\nLuciferase Stable WM-266-4 Cell Line<\/td>\n<\/tr>\n
T3015<\/td>\nNF-kB Luciferase Stable RAW264.7 Cell Line<\/td>\n<\/tr>\n
T3016<\/td>\nHEK293-CymR-Puro Stable Cell Line<\/td>\n<\/tr>\n
T3017<\/td>\nA2058-CymR Stable Cell Line<\/td>\n<\/tr>\n
T3018<\/td>\nSKMEL5-CymR Stable Cell Line<\/td>\n<\/tr>\n
T3021<\/td>\nGRPR-Luciferase Stable PC3 Cell Line<\/td>\n<\/tr>\n
T3024<\/td>\nBax Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3025<\/td>\nBak Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3026<\/td>\nBax\/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3027<\/td>\nAtg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3028<\/td>\nAtg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3030<\/td>\nNav2.1\/&beta;1\/Contactin Stable Chinese Hamster Lung Cell Line<\/td>\n<\/tr>\n
T3031<\/td>\nPARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line<\/td>\n<\/tr>\n
T3032<\/td>\nDual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line<\/td>\n<\/tr>\n
T3033<\/td>\nDual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4&lt;sup&gt;esn&lt;\/sup&gt;) Cell Line<\/td>\n<\/tr>\n
T3034<\/td>\nTLR3 Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3035<\/td>\nTLR9 Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3036<\/td>\nType I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3037<\/td>\nRyanodine Receptor 1 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3038<\/td>\nKCC2 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3039<\/td>\nStable HEK293 Expressing Mutant UQCRB-MT1 Cell Line<\/td>\n<\/tr>\n
T3040<\/td>\nc-Kit\/D816V Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3041<\/td>\nc-Kit\/WT Stable Ba\/F3 (with GNNK- isoform) Cell Line<\/td>\n<\/tr>\n
T3042<\/td>\nc-Kit\/WT Stable Ba\/F3 (with GNNK+ isoform) Cell Line<\/td>\n<\/tr>\n
T3043<\/td>\nc-Kit\/Y568F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3044<\/td>\nc-Kit\/Y570F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3045<\/td>\nc-Kit Y568F\/Y570F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3046<\/td>\nc-Kit\/Y703F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3047<\/td>\nc-Kit\/Y936F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3048<\/td>\nc-Kit Y703F\/Y936F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3049<\/td>\nc-Kit\/N705AStable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3050<\/td>\nc-Kit\/N938A Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3051<\/td>\nc-Kit N705A\/N938A Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3052<\/td>\nc-Kit\/D816H Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3053<\/td>\nc-Kit\/D816F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3054<\/td>\nc-Kit\/D816YStable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3055<\/td>\nc-Kit\/V560D Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3056<\/td>\nc-Kit\/K642E Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3057<\/td>\nc-Kit\/Y721F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3058<\/td>\nc-Kit\/Y823F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3059<\/td>\nTLR2 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3060<\/td>\nPGC-1\u03b1\/ERR\u03b1 Stable 293T Cell Line<\/td>\n<\/tr>\n
T3061<\/td>\nTNK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3062<\/td>\nALK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3063<\/td>\nLTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3064<\/td>\nAXL Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3065<\/td>\nMERTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3066<\/td>\nTYRO3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3067<\/td>\nEPHA1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3068<\/td>\nEPHA5 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3069<\/td>\nEPHA7 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3070<\/td>\nEPHA8 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3071<\/td>\nFGFR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3072<\/td>\nFGFR3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3073<\/td>\nIGF1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3074<\/td>\nINSR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3075<\/td>\nJAK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3076<\/td>\nJAK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3077<\/td>\nJAK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3078<\/td>\nMET Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3079<\/td>\nMST1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3080<\/td>\nFLT3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3081<\/td>\nKIT Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3082<\/td>\nPDGFRA Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3083<\/td>\nPDGFRB Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3084<\/td>\nRET Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3085<\/td>\nROS1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3086<\/td>\nBLK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3087<\/td>\nFGR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3088<\/td>\nFYN Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3089<\/td>\nHCK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3090<\/td>\nLCK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3091<\/td>\nSRC Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3092<\/td>\nYES1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3093<\/td>\nSYK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3094<\/td>\nNTRK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3095<\/td>\nNTRK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3096<\/td>\nNTRK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3097<\/td>\nFLT4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3098<\/td>\nERBB2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3099<\/td>\nEPHA4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3100<\/td>\nABL1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3101<\/td>\nABL2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3102<\/td>\nAZ-AHR Stable HepG2 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3103<\/td>\nAZ-GR Stable HeLa Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3104<\/td>\nAIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3105<\/td>\nBMP Responsive Immortalized Reporter (BRITER) Cell Line<\/td>\n<\/tr>\n
T3106<\/td>\nEGFR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3107<\/td>\nERBB4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3108<\/td>\nEPHA2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3109<\/td>\nEPHB1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3110<\/td>\nEPHB2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3111<\/td>\nTYK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3112<\/td>\nEPHB4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3113<\/td>\nFER Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3115<\/td>\nLYN Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3116<\/td>\nZAP70 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3117<\/td>\nTIE1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3118<\/td>\nKDR Stable Ba\/F3 Cell Line (IL-3 Independent)<\/td>\n<\/tr>\n
T3119<\/td>\nTNK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3120<\/td>\nCSK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3121<\/td>\nMATK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3122<\/td>\nDDR1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3123<\/td>\nDDR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3124<\/td>\nPTK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3126<\/td>\nEPHA10 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3127<\/td>\nEPHA3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3128<\/td>\nEPHA6 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3129<\/td>\nEPHB3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3131<\/td>\nPTK2B Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3132<\/td>\nFGFR4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3133<\/td>\nINSRR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3134<\/td>\nAATK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3136<\/td>\nLMTK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3137<\/td>\nMuSK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3138<\/td>\nCSF1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3139<\/td>\nPTK7 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3140<\/td>\nROR1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3141<\/td>\nROR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3142<\/td>\nRYK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3143<\/td>\nFRK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3145<\/td>\nSRMS Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3146<\/td>\nBMX Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3147<\/td>\nBTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3148<\/td>\nITK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3149<\/td>\nTEC Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3150<\/td>\nTXK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3151<\/td>\nTEK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3152<\/td>\nFLT1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3153<\/td>\nSTYK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3154<\/td>\nSPINK1 Stable AsPC-1 Cell Line<\/td>\n<\/tr>\n
T3155<\/td>\nhSSAO\/VAP-1 A7r5 Stable Cell Line<\/td>\n<\/tr>\n
T3156<\/td>\nMouse R-spondin1 Stable 293T Cell Line<\/td>\n<\/tr>\n
T3157<\/td>\nHuman T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line<\/td>\n<\/tr>\n
T3158<\/td>\nTrkA Stable T17 Cell Line<\/td>\n<\/tr>\n
T3159<\/td>\nTrkB Stable T48 Cell Line<\/td>\n<\/tr>\n
T3160<\/td>\nLuc-Akt-PH Stable MCF7 Cell Line<\/td>\n<\/tr>\n
T3161<\/td>\nLuc-Akt-PH\/YFP Stable MCF-7\/B2 Stable Cell Line<\/td>\n<\/tr>\n
T3162<\/td>\nHEK293S-TetR Stably Expressing TP Cell Line<\/td>\n<\/tr>\n
T3163<\/td>\nHEK293S-TetR GnTI- Stably Expressing TP Cell Line<\/td>\n<\/tr>\n
T3164<\/td>\nHEK293S-TetR Stably Expressing A160T Cell Line<\/td>\n<\/tr>\n
T3165<\/td>\nHEK293S-TetR GnTI- Stably Expressing A160T Cell Line<\/td>\n<\/tr>\n
T3166<\/td>\nCx26 Stable BxPC-3 Cell Line<\/td>\n<\/tr>\n
T3167<\/td>\nE-cadherin Stable MIA PaCa-2 Cell Line<\/td>\n<\/tr>\n
T3168<\/td>\nBMP9 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3169<\/td>\n92 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line<\/td>\n<\/tr>\n
T3170<\/td>\n88 Tag Stable Y-LIZ DNA Polymerase-\u03b2 Knockout Mouse Embryonic Fibroblast Cell Line<\/td>\n<\/tr>\n
T3171<\/td>\nSIRT1 Wiltype Stable RKO Cell Line<\/td>\n<\/tr>\n
T3172<\/td>\nSIRT1 Knockout Stable RKO Cell Line<\/td>\n<\/tr>\n
T3173<\/td>\nSIRT1 Wildtype Stable DLD-1 Cell Line<\/td>\n<\/tr>\n
T3174<\/td>\nSIRT1 Knockout Stable DLD-1 Cell Line<\/td>\n<\/tr>\n
T3175<\/td>\nSIRT1 Wildtype Stable 293T Cell Line<\/td>\n<\/tr>\n
T3176<\/td>\nSIRT1 Knockout Stable 293T Cell Line<\/td>\n<\/tr>\n
T3177<\/td>\nPZ-TR Stable HepG2 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3178<\/td>\nAd5 pTP Stable 293 (293pTP) Cell Line<\/td>\n<\/tr>\n
T3181<\/td>\np47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line<\/td>\n<\/tr>\n
T3182<\/td>\nPodoplanin Stably Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3183<\/td>\nStable Retinal Pigment Epithelial Expressing Truncated hELG1-CFP Cell Line<\/td>\n<\/tr>\n
T3184<\/td>\nStable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line<\/td>\n<\/tr>\n
T3185<\/td>\nStable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line<\/td>\n<\/tr>\n
T3186<\/td>\nStable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line<\/td>\n<\/tr>\n
T3187<\/td>\nStable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line<\/td>\n<\/tr>\n
T3188<\/td>\nStable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line<\/td>\n<\/tr>\n
T3189<\/td>\nStable VERO Overexpressing HER2 Cell Line<\/td>\n<\/tr>\n
T3190<\/td>\nStable VERO Overexpressing HER3 Cell Line<\/td>\n<\/tr>\n
T3191<\/td>\nStable VERO Overexpressing HER2 and HER3 Cell Line<\/td>\n<\/tr>\n
T3192<\/td>\nStable VERO Overexpressing HER1 Cell Line<\/td>\n<\/tr>\n
T3193<\/td>\nStable CHO Overexpressing HER2 Cell Line<\/td>\n<\/tr>\n
T3194<\/td>\nStable VERO Overexpressing HER1 and HER2 Cell Line<\/td>\n<\/tr>\n
T3195<\/td>\nStable HepG2 Expressing Murine ADH1 (VA-13) Cell Line<\/td>\n<\/tr>\n
T3196<\/td>\nStable HA-Tagged hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3197<\/td>\nStable G90E Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3198<\/td>\nStable V94M Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3199<\/td>\nStable C307Y Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3203<\/td>\nCRE Expressing 293 Cell Line<\/td>\n<\/tr>\n
T3207<\/td>\nICP47 Stable Raji Cell Line<\/td>\n<\/tr>\n
T3208<\/td>\nICP47 Stable Mel JuSo Cell Line<\/td>\n<\/tr>\n
T3209<\/td>\nHLA-DQ8 Expressing Stable K562 Cell Line<\/td>\n<\/tr>\n
T3210<\/td>\nMutant AKT1 Stable Proteus Syndrome Derived Cell Line (F6B)<\/td>\n<\/tr>\n
T3217<\/td>\nGFP Stably Expressing Schwann cells<\/td>\n<\/tr>\n
T3218<\/td>\nhuGRPr Expessing Stable Ace-1 Cell Line<\/td>\n<\/tr>\n
T3219<\/td>\nApolipoprotein A-1 Expressing Stable MC38 Cell Line (MC38 Apo)<\/td>\n<\/tr>\n
T3220<\/td>\nApolipoprotein A-1 and P144 Over-expressing Stable MC38 Cell Line (MC38 ApoLinkerP144)<\/td>\n<\/tr>\n
T3222<\/td>\nBRCA1 L1780P Mutant Stable HCC1937 Cell Line<\/td>\n<\/tr>\n
T3223<\/td>\nBRCA1 L1780P Mutant Stable HCC1395 TNBC Cell Line<\/td>\n<\/tr>\n
T3224<\/td>\nNDUFS2 Stable Knockout HEK293 Cell Line (HEK293\u0394NDUFS2)<\/td>\n<\/tr>\n
T3229<\/td>\nHEK293-T Cells Stably Expressing MLL-AF6 (MA6)<\/td>\n<\/tr>\n
T3230<\/td>\nHEK293-T Cells Stably Expressing AF6-MLL (A6M)<\/td>\n<\/tr>\n
T3231<\/td>\nHEK293-T Cells Stably Expressing MLL-AF6\/AF6-MLL (CO1)<\/td>\n<\/tr>\n
T3232<\/td>\nHEK293-T Cells Stably Expressing exMLL-AF6 (exMA6)<\/td>\n<\/tr>\n
T3233<\/td>\nHEK293-T Cells Stably Expressing AF6-shMLL (A6shM)<\/td>\n<\/tr>\n
T3234<\/td>\nHEK293-T Cells Stably Expressing exMLL-AF6\/AF6-shMLL (CO2)<\/td>\n<\/tr>\n
T3235<\/td>\nA549 Cells Stably Expressing hACE2 Receptor and TMPRSS2 (A549-AT)<\/td>\n<\/tr>\n
T3236<\/td>\nU2OS Stably Expressing NONO-APEX2 Cell Line (U2OS:NONO-APEX2-HA)<\/td>\n<\/tr>\n
T3241<\/td>\nts-46 Stable Knockout HEK293 Cell Line (ts-46 293KO)<\/td>\n<\/tr>\n
T3242<\/td>\nts-101 Stable Knockout HEK293 Cell Line (ts-101 293KO)<\/td>\n<\/tr>\n
T3243<\/td>\nHuman Progerin Expressing 3T3-L1 Stable Cell Line (3T3L1-PG)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u7cfb\u5217\u5546\u54c1 (immortalized cell)-\u6c38\u751f\u5316SVOG\u7d30\u80de\u682a SVOG Cell […]<\/p>\n","protected":false},"author":3,"featured_media":28511,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,7018],"tags":[6610],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49730"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49730"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49730\/revisions"}],"predecessor-version":[{"id":50770,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49730\/revisions\/50770"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28511"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49730"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=49730"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=49730"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}