{"id":49707,"date":"2024-06-25T16:30:11","date_gmt":"2024-06-25T08:30:11","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49707"},"modified":"2024-07-18T18:00:00","modified_gmt":"2024-07-18T10:00:00","slug":"%e5%96%ae%e6%a0%b8%e7%90%83%e7%b4%b0%e8%83%9e%e5%88%86%e9%9b%a2%e6%b6%b2-lymphoprep-density-gradient-media%e8%b2%a8%e8%99%9fpg-1858-6","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49707","title":{"rendered":"\u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2 | Lymphoprep Density Gradient Media\u8ca8\u865fPG-1858-6"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=49707&amp;preview=true\"><span style=\"font-size: 12pt;\">\u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2 | Lymphoprep Density Gradient Media\u8ca8\u865fPG-1858-6<\/span><\/a><\/span><\/p>\n<p>Lymphoprep\u2122 \u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2 (Lymphoprep Density Gradient Media, \u8ca8\u865fPG-185-6) \u662f\u4e00\u7a2e\u5373\u7528\u578b\u3001\u7121\u83cc\u4e14\u7d93\u904e\u5167\u6bd2\u7d20\u6e2c\u8a66\u7684\u6e2c\u8a66\u6eb6\u6db2\uff0c\u7528\u65bc\u5206\u96e2\u7d14\u6dcb\u5df4\u7d30\u80de\u61f8\u6d6e\u6db2(pure lymphocyte suspensions)\u3002\u6eb6\u6db2\u542b\u6709\u4ee5\u4e0b\u6fc3\u5ea6\u7684 Sodium Diatrizoate\u548c\u591a\u91a3(Polysaccharide)\uff1a<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/2-%E5%96%AE%E6%A0%B8%E7%90%83%E7%B4%B0%E8%83%9E%E5%88%86%E9%9B%A2%E6%B6%B2-Lymphoprep-Density-Gradient-Media%E8%B2%A8%E8%99%9FPG-1858-6.png\" \/><\/p>\n<h1><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2\u539f\u7406 <\/span><\/strong><\/span><\/h1>\n<p>\u5206\u96e2\u767d\u8840\u7403\u6700\u5e38\u898b\u7684\u6280\u8853\u662f\u5c07\u8840\u6db2\u8207\u805a\u96c6(aggregates )\u7d05\u8840\u7403\u7684\u5316\u5408\u7269\u6df7\u5408\uff0c\u5f9e\u800c\u589e\u52a0\u5176\u6c89\u6fb1\u901f\u7387(sedimentation rate)\u3002\u767d\u8840\u7403(leucocytes)\u7684\u6c89\u964d\u50c5\u53d7\u5230\u8f15\u5fae\u5f71\u97ff\uff0c\u7576\u7d05\u8840\u7403\u6c89\u964d\u6642\uff0c\u53ef\u4ee5\u5f9e\u7ba1\u7684\u4e0a\u90e8\u6536\u96c6\u767d\u8840\u7403\u3002 B\u00f8yum (1968) \u4f7f\u7528 Metrizoate \u548c\u591a\u91a3\u7684\u6df7\u5408\u7269\u958b\u767c\u4e86\u4e00\u7a2e\u4e00\u6b65\u5f0f\u96e2\u5fc3\u6280\u8853\uff0c\u7528\u65bc\u5f9e\u5ba4\u6eab\u4e0b\u4fdd\u5b58\u9577\u9054 6 \u5c0f\u6642\u7684\u6297\u51dd\u8840\u8840\u6db2\u4e2d\u5206\u96e2\u6dcb\u5df4\u7d30\u80de\u3002Lymphoprep\u2122\u53ef\u7a69\u5b9a\u4fdd\u5b58 3 \u5e74(\u4fdd\u6301\u7121\u83cc\u4e26\u907f\u5149)\u3002\u9577\u6642\u9593\u66b4\u9732\u5728\u967d\u5149\u76f4\u5c04\u4e0b\u6703\u5c0e\u81f4\u6cdb\u5f71\u9178\u9209\u5206\u5b50\u91cb\u653e\u7898\u3002\u3002 Lymphoprep\u2122 \u61c9\u5132\u5b58\u65bc +4 \u00b0C \u81f3 +30 \u00b0C \u4e0b\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" id=\"img-R-type-2\" class=\"\" src=\"https:\/\/www.blossombio.com\/img\/PRODUCT\/Lymphoprep\/Lymphoprep.jpg\" alt=\"Lymphoprep\u2122 \u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2\" width=\"372\" height=\"293\" \/><\/p>\n<p>\u5bc6\u5ea6\u68af\u5ea6\u96e2\u5fc3 (Density gradient centrifugation) \u662f\u4ee5\u4e00\u5b9a\u4ecb\u8cea\u5728\u96e2\u5fc3\u7ba1\u5167\u5f62\u6210\u5bc6\u5ea6\u68af\u5ea6\uff0c\u85c9\u4ee5\u5206\u96e2\u7d30\u80de\u6216\u80de\u5668\u7684\u5be6\u9a57\u65b9\u5f0f\u3002Lymphoprep\u2122 \u70ba\u9069\u7528\u65bc\u5206\u96e2\u4eba\u985e\u8840\u6db2\u6dcb\u5df4\u7403 (Lymphocytes) \u53ca\u55ae\u6838\u7403 (Monocytes) \u7684\u7406\u60f3\u4ecb\u8cea\uff0c\u5177\u5099\u4ee5\u4e0b\u7279\u9ede\uff1a<\/p>\n<ul>\n<li>\u5bc6\u5ea6 1.077\u00b10.001 g\/ml\uff0c\u53ef\u53d6\u4ee3\u4ed6\u724c Plus \u6216 PREMIUM \u7522\u54c1<\/li>\n<li>\u64cd\u4f5c\u65b9\u4fbf\uff0c\u5229\u7528\u7c21\u55ae\u7684\u5bc6\u5ea6\u68af\u5ea6\u96e2\u5fc3\u65b9\u5f0f\u534a\u5c0f\u6642\u5167\u5373\u53ef\u5b8c\u6210\u7d30\u80de\u5206\u96e2<\/li>\n<li>\u5177\u7b49\u6ef2\u900f\u58d3\u53ca\u9069\u5408\u7684 pH\uff0c\u4e0d\u5c0d\u6a23\u672c\u9020\u6210\u7834\u58de<\/li>\n<li>\u4f4e\u9ecf\u6027\u3001\u7121\u6bd2\u6027\uff0c\u7b26\u5408 GMP \u898f\u7bc4<\/li>\n<li>\u50f9\u683c\u5177\u6709\u9ad8\u5ea6\u7af6\u722d\u529b<\/li>\n<\/ul>\n<p><strong><span style=\"color: #800000; font-size: 12pt;\">Lymphoprep\u2122 \u64cd\u4f5c\u6d41\u7a0b\uff08\u898b\u5716\uff09<\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/4-%E5%96%AE%E6%A0%B8%E7%90%83%E7%B4%B0%E8%83%9E%E5%88%86%E9%9B%A2%E6%B6%B2-Lymphoprep-Density-Gradient-Media%E8%B2%A8%E8%99%9FPG-1858-6.png\" \/><\/p>\n<p>1. \u5c07\u8840\u6db2\u6536\u96c6\u5230\u542b\u6709\u6297\u51dd\u8840\u5291 (EDTA, heparin, ACD)\u7684\u8a66\u7ba1\u4e2d\u6216\u4f7f\u7528\u53bb\u7e96\u7dad\u8840\u6db2(defibrinated blood)\u3002<\/p>\n<p>2. \u52a0\u5165\u7b49\u9ad4\u7a4d ( 1:1 \u7a00\u91cb ) \u7684 0.9% NaCl \u7a00\u91cb\u8840\u6db2\u3002<\/p>\n<p>3. \u5c0f\u5fc3\u5730\u5c07 6 ml \u7a00\u91cb\u8840\u6db2\u7f6e\u65bc 12\u201315 mm \u96e2\u5fc3\u7ba1\u4e2d\u7684 3 ml Lymphoprep\u2122 \u4e0a\u3002\u6216\u8005\uff0cLymphoprep\u2122 \u53ef\u4ee5\u92ea\u5728\u5e95\u5c64\u3002\u518d\u5c07 6 ml \u7a00\u91cb\u5f8c\u7684\u8840\u6db2\u6a23\u672c\u52a0\u81f3\u4e0a\u5c64\u3002<br \/>\n\u907f\u514d\u8840\u6db2\u548c\u5206\u96e2\u6db2\u6df7\u5408\u3002\u84cb\u4f4f\u7ba1\u5b50\u4ee5\u9632\u6b62\u5f62\u6210\u6c23\u6eb6\u81a0\u3002<\/p>\n<p>4. \u5728\u5ba4\u6eab\uff08\u7d04 20 \u00b0C\uff09\u4e0b\uff0c 800 x g \u96e2\u5fc3 20 \u5206\u9418\u3002\u5982\u679c\u8840\u6db2\u4fdd\u5b58\u6642\u9593\u8d85\u904e2\u5c0f\u6642\uff0c\u8acb\u5c07\u96e2\u5fc3\u6642\u9593\u589e\u52a0\u81f330\u5206\u9418\u3002<\/p>\n<p>5. \u96e2\u5fc3\u5f8c\uff0c\u55ae\u6838\u7d30\u80de\u5728\u6a23\u672c\/\u4ecb\u8cea\u754c\u9762\u8655\u5f62\u6210\u660e\u986f\u7684\u689d\u5e36\uff0c\u5982\u5716\u6240\u793a\u3002\u6700\u597d\u4f7f\u7528\u5df4\u65af\u5fb7\u79fb\u6db2\u7ba1\u5f9e\u754c\u9762\u4e0a\u53bb\u9664\u7d30\u80de\uff0c\u800c\u4e0d\u53bb\u9664\u4e0a\u5c64\u3002<\/p>\n<p>6. \u4ee5 0.9% NaCl \u6216\u57f9\u990a\u57fa\u7a00\u91cb\u6536\u7a6b\u7684\u7d1a\u5206\u4ee5\u964d\u4f4e\u6eb6\u6db2\u5bc6\u5ea6\uff0c\u4e26\u4ee5 250 x g \u96e2\u5fc3 10 \u5206\u9418\u6c89\u6fb1\u7d30\u80de\u3002<\/p>\n<p><strong><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/1-\u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2-Lymphoprep-Density-Gradient-Media\u8ca8\u865fPG-1858-6.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7d14\u5ea6\u548c\u6d3b\u6027(PURITY AND VIABILITY)<\/span><\/strong><\/span><\/p>\n<p>\u5df2\u767c\u73fe\u6240\u63cf\u8ff0\u7684\u65b9\u6cd5\u5feb\u901f\u3001\u7c21\u55ae\u4e14\u53ef\u9760\uff0c\u4e26\u4e14\u5c0d\u4f86\u81ea\u5927\u591a\u6578\u6b63\u5e38\u500b\u9ad4\u548c\u60a3\u8005\u7684\u8840\u6db2\u6a23\u672c\u7d66\u51fa\u4e86\u512a\u7570\u7684\u7d50\u679c\u3002\u8a72\u6280\u8853\u9084\u53ef\u7528\u65bc\u88fd\u5099\u7528\u65bc\u6df7\u5408\u6dcb\u5df4\u7d30\u80de\u57f9\u990a\u6e2c\u8a66\u7684\u6dcb\u5df4\u7d30\u80de\u61f8\u6d6e\u6db2\u3002\u7d05\u8840\u7403\u6dcb\u5df4\u7403\u61f8\u6d6e\u6db2\u4e2d\u7684\u6c61\u67d3\u901a\u5e38\u4f54\u7e3d\u7d30\u80de\u6578\u7684 1-5%\u3002\u5728\u5f37\u70c8\u7684\u514d\u75ab\u6291\u5236\u6cbb\u7642\u671f\u9593\uff0c\u4e00\u4e9b\u672a\u6210\u719f\u7684\u7c92\u7d30\u80de\u53ef\u80fd\u6703\u8ddf\u96a8\u6dcb\u5df4\u7d30\u80de\u3002\u7576\u4f7f\u7528\u809d\u7d20\u5316\u8840\u6db2\u6642\uff0c\u5fc5\u9808\u79fb\u9664\u5927\u90e8\u5206\u8840\u5c0f\u677f\uff0c\u4ee5\u907f\u514d\u7d30\u80de\u6bd2\u6027\u6e2c\u8a66\u4e2d\u7684\u6291\u5236\u3002\u6240\u63cf\u8ff0\u7684\u6d17\u6ecc\u7a0b\u5e8f\u901a\u5e38\u5c31\u8db3\u5920\u4e86\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u55ae\u6838\u7403\u7d30\u80de\u5206\u96e2\u6db2 | Lymphoprep Density Gradient Media\u8ca8\u865fPG-1858-6 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":49708,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6609],"tags":[6633],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49707"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49707"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49707\/revisions"}],"predecessor-version":[{"id":49709,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49707\/revisions\/49709"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/49708"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49707"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=49707"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=49707"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}