{"id":49693,"date":"2024-06-24T13:06:41","date_gmt":"2024-06-24T05:06:41","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49693"},"modified":"2024-07-18T18:00:21","modified_gmt":"2024-07-18T10:00:21","slug":"%e8%84%82%e8%82%aa%e9%85%b8%e6%94%9d%e5%8f%96%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-fatty-acid-uptake-assay-kit-%e8%b2%a8%e8%99%9fabnovaka4084","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49693","title":{"rendered":"\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| Fatty Acid Uptake Assay Kit \u8ca8\u865fAbnova\u2122KA4084"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><strong><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=49693&amp;preview=true\"><span style=\"font-size: 12pt;\">\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| Fatty Acid Uptake Assay Kit \u8ca8\u865fAbnova\u2122KA4084<\/span><\/a><\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/abnova.png\" width=\"628\" height=\"82\" \/><\/p>\n<p><span style=\"color: #999999;\">100 assays\u00a0<\/span><\/p>\n<p>\u8102\u80aa\u9178\u651d\u53d6(Fatty acid uptake )\u662f\u6cbb\u7642\u80a5\u80d6\u3001\u7b2c2\u578b\u7cd6\u5c3f\u75c5( type 2 diabete )\u548c\u809d\u8102\u80aa\u8b8a\u6027\u7b49\u8a31\u591a\u4eba\u985e\u75be\u75c5\u7684\u91cd\u8981\u6cbb\u7642\u6a19\u9776\u3002 \u6e2c\u5b9a\u539f\u7406 \u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u800c\u9748\u654f\u7684\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u542b\u6709\u8102\u80aa\u9178\u8f49\u904b\u86cb\u767d(fatty acid transporters)\u7684\u7d30\u80de\u4e2d\u7684\u8102\u80aa\u9178\u651d\u53d6\u3002\u672c\u8a66\u5291\u76d2\u4f7f\u7528\u5c08\u6709\u7684\u5341\u4e8c\u70f7\u9178\u87a2\u5149\u8102\u80aa\u9178\u5e95\u7269\u3002\u6b64\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u5728\u4efb\u4f55\u5177\u6709\u5e95\u90e8\u8b80\u53d6\u6a21\u5f0f\u6216 FITC \u901a\u9053\u7684\u87a2\u5149\u9176\u6a19\u5100\u4e0a\u9032\u884c\u3002\u6b64\u6e2c\u5b9a\u53ef\u4ee5\u5728 96 \u5b54\u6216 384 \u5b54\u5fae\u91cf\u6ef4\u5b9a\u677f\u4e2d\u900f\u904e\u7c21\u55ae\u7684\u6df7\u5408\u548c\u8b80\u53d6\u7a0b\u5e8f\u9032\u884c\uff0c\u4e26\u4e14\u53ef\u4ee5\u8f15\u9b06\u9069\u61c9\u9ad8\u901a\u91cf\u7be9\u9078\u61c9\u7528\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/2-%E8%84%82%E8%82%AA%E9%85%B8%E6%94%9D%E5%8F%96%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Fatty-Acid-Uptake-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2KA4084.png\" \/><\/p>\n<p>\u6e2c\u5b9a\u7a0b\u5e8f<\/p>\n<p>\u4f9d\u9700\u8981\u7528\u6e2c\u8a66\u5316\u5408\u7269\u8655\u7406\u7d30\u80de\u3002 \u5f9e\u57f9\u990a\u7bb1\u4e2d\u53d6\u51fa\u7d93\u5316\u5408\u7269\u8655\u7406\u7684\u7d30\u80de\u677f\uff0c\u52a0\u5165 100 \u00b5L\/\u5b54\uff0896 \u5b54\u677f\uff09\u6216 25 \u00b5L\/\u5b54\uff08384 \u5b54\u677f\uff09\uff08\u5305\u62ec\u7a7a\u767d\u5b54\uff09\u8102\u80aa\u9178\u67d3\u6599\u4e0a\u6a23\u6eb6\u6db2\u3002\u4f7f\u7528\u5e95\u90e8\u8b80\u53d6\u6a21\u5f0f\uff0c\u4f7f\u7528\u87a2\u5149\u9176\u6a19\u5100\u5728 Ex\/Em = 485\/515 nm\uff08\u5728 495 nm \u622a\u6b62\uff09\u6e2c\u91cf\u87a2\u5149\u8a0a\u865f\u3002<\/p>\n<p>\u5c0d\u65bc\u52d5\u529b\u5b78\u8b80\u6578\uff1a\u7acb\u5373\u4ee5 20 \u79d2\u7684\u9593\u9694\u8b80\u53d6\u87a2\u5149\u5f37\u5ea6\uff0c\u6301\u7e8c 30-60 \u5206\u9418\u3002<\/p>\n<p>\u5c0d\u65bc\u7d42\u9ede\u8b80\u6578\uff1a\u5728 30-60 \u5206\u9418\u5b75\u80b2\u7d50\u675f\u6642\u8b80\u53d6\u87a2\u5149\u5f37\u5ea6\u3002<\/p>\n<p>\u5c07\u7d30\u80de\u5728\u751f\u9577\u57f9\u990a\u57fa\u4e2d\u57f9\u990a4-6 \u5c0f\u6642\u5c07\u7d30\u80de\u8f49\u79fb\u81f3\u7121\u8840\u6e05\u57f9\u990a\u57fa\u4e2d1 \u5c0f\u6642\uff0c\u4e26\u6839\u64da\u9700\u8981\u8655\u7406\u7d30\u80de\u6dfb\u52a0100 \u03bcL\/\u5b54\u7684\u8102\u80aa\u9178\u67d3\u6599\u4e0a\u6a23\u6eb6\u6db2\u76e3\u6e2c\u87a2\u5149\u7acb\u5373\u5728Ex\/Em = 485\/515 nm \u8655\u589e\u52a0\u4ee5\u9032\u884c\u52d5\u529b\u5b78\u5206\u6790\uff0c\u6216\u5728\u5b75\u80b260 \u5206\u9418\u5f8c\u9032\u884c\u7d42\u9ede\u8b80\u53d6\uff08\u5e95\u90e8\u8b80\u53d6\u6a21\u5f0f\uff09<\/p>\n<p>\u91cd\u8981\u63d0\u793a\uff1a\u5728\u958b\u59cb\u5be6\u9a57\u4e4b\u524d\uff0c\u8acb\u5728\u5ba4\u6eab\u4e0b\u89e3\u51cd\u6240\u6709\u8a66\u5291\u76d2\u7d44\u4ef6\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/3-%E8%84%82%E8%82%AA%E9%85%B8%E6%94%9D%E5%8F%96%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92Fatty-Acid-Uptake-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2KA4084.png\" \/><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">3T3-L1\u8102\u80aa\u7d30\u80de\u548c\u7e96\u7dad\u6bcd\u7d30\u80de\u8102\u80aa\u9178\u651d\u53d6\u7684\u6bd4\u8f03\u3002<\/span><\/strong><\/span><\/p>\n<p>\u5c07\u7d30\u80de\u4ee550,000\u500b\u7d30\u80de\/100uL\/\u5b54\u92ea\u572896\u5b54\u9ed1\u58c1\/\u900f\u660e\u5e95\u90e8\u805a-D\u8cf4\u6c28\u9178\u677f\u4e2d5\u5c0f\u6642\uff0c\u7136\u5f8c\u53bb\u8840\u6e051\u5c0f\u6642\u3002\u7d30\u80de\u4e0d\u4f7f\u7528\uff08\u5c0d\u7167\uff09\u6216\u7528\u80f0\u5cf6\u7d20\uff08150 nM\uff09\u8655\u7406\uff0c\u4e26\u5728 37\u00b0C\u30015% CO2 \u57f9\u990a\u7bb1\u4e2d\u5b75\u80b2 30 \u5206\u9418\u3002\u5b75\u80b2\u6642\u9593\u7d50\u675f\u6642\uff0c\u5c07100ul\u8102\u80aa\u9178\u6df7\u5408\u7269\u52a0\u5165\u5b54\u4e2d\uff0c\u4e26\u518d\u5b75\u80b260\u5206\u9418\uff0c\u4f7f\u7528FlexStation\u8b80\u677f\u5668\u4f7f\u7528\u5e95\u90e8\u8b80\u53d6\u6a21\u5f0f\u6e2c\u91cf\u87a2\u5149\u8a0a\u865f\u3002 A\uff1a\u7e96\u7dad\u6bcd\u7d30\u80de\uff08\u5c0d\u7167\uff09\uff1b B\uff1a\u7e96\u7dad\u6bcd\u7d30\u80de\uff08\u80f0\u5cf6\u7d20\uff09\uff1b C\uff1a\u8102\u80aa\u7d30\u80de\uff08\u5c0d\u7167\uff09\uff1b D\uff1a\u8102\u80aa\u7d30\u80de\uff08\u80f0\u5cf6\u7d20\uff09\u3002\u5f9e\u542b\u6709\u7d30\u80de\u7684\u5b54\u7684\u503c\u4e2d\u6e1b\u53bb\u542b\u6709\u751f\u9577\u57f9\u990a\u57fa\u7684\u7a7a\u767d\u5b54\u4e2d\u7684\u87a2\u5149\u3002\u7a7a\u767d\u5b54\u7684\u80cc\u666f\u87a2\u5149\u53ef\u80fd\u6703\u6839\u64da\u751f\u9577\u57f9\u990a\u57fa\u6216\u5fae\u91cf\u6ef4\u5b9a\u677f\u7684\u4f86\u6e90\u800c\u6539\u8b8a\u3002<\/p>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Product Description<\/span><\/h4>\n<p>Fatty Acid Uptake Assay Kit is used for the measurement of fatty acid uptake.<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Suitable Sample<\/span><\/h4>\n<p>Live Cells<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\">Excitation\/&lt;br&gt;Emission (nm)<\/h4>\n<p>485\/515 nm<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Detection Method<\/span><\/h4>\n<p>Fluorometric<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Instrument Platform<\/span><\/h4>\n<p>Fluorescence Microscope, Microplate Readers<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Regulation Status<\/span><\/h4>\n<p>For research use only (RUO)<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"font-size: 12pt; color: #800000;\">Storage Instruction<\/span><\/h4>\n<p>Store the kit in desiccated environment at -20\u00b0C and avoid from light.<\/p>\n<\/div>\n<div class=\"article-block\">\n<h4 class=\"font-18 semiblod\"><span style=\"color: #800000;\">Note<\/span><\/h4>\n<p>Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.<\/p>\n<\/div>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u61c9\u7528\uff1a <\/span><\/strong><\/span><\/p>\n<pre id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"\u7ffb\u8b6f\" data-ved=\"2ahUKEwjHsIzg2ZWCAxVNhlYBHYHMDksQ3ewLegQIBhAQ\"><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713 \u61c9\u7528\uff1a \r\n\u6aa2\u6e2c\uff1a\u751f\u7269\u6a23\u672c\u4e2d\u7684\u78f7\u8102\uff0c\u4f8b\u5982\u8840\u6e05\u548c\u975e EDTA \u8840\u6f3f\u3002\u85e5\u7269\u767c\u73fe\/\u85e5\u7406\u5b78\uff1a\u85e5\u7269\u5c0d\u542b\u81bd\u9e7c\u78f7\u8102\u4ee3\u8b1d\u7684\u5f71\u97ff\u3002 \r\n<\/span><\/pre>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7279\u9ede\uff1a <\/span><\/strong><\/span><\/p>\n<pre class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"\u7ffb\u8b6f\" data-ved=\"2ahUKEwjHsIzg2ZWCAxVNhlYBHYHMDksQ3ewLegQIBhAQ\"><span class=\"Y2IQFc\" lang=\"zh-TW\">\u2713\u4e3b\u8981\u7279\u6027 \u9748\u654f\uff1a\u4f7f\u7528 20 \u00b5L \u6a23\u54c1\u3002\u7dda\u6027\u5075\u6e2c\u7bc4\u570d\uff1a\u6bd4\u8272\u6e2c\u5b9a 3 - 200 \u03bcM\uff0c\u87a2\u5149\u6e2c\u5b9a 0.6 - 20 \u03bcM \u78f7\u8102\u8cea\u3002\r\n\r\n<\/span><\/pre>\n<p><a href=\" http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2024\/06\/1-\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2-Fatty-Acid-Uptake-Assay-Kit-\u8ca8\u865fAbnova\u2122KA4084.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<div class=\"article-block\">\n<div class=\"article-block\">\n<div class=\"article-block\">\n<div class=\"article-block\">\n<div class=\"article-block\">\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u8102\u80aa\u9178\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| Fatty Acid Uptake Assay Kit \u8ca8\u865fAbnova\u2122KA4084 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6636],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49693"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49693"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49693\/revisions"}],"predecessor-version":[{"id":49694,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49693\/revisions\/49694"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49693"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=49693"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=49693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}