{"id":49682,"date":"2024-06-21T16:06:23","date_gmt":"2024-06-21T08:06:23","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49682"},"modified":"2024-07-18T18:00:34","modified_gmt":"2024-07-18T10:00:34","slug":"human-type-iv-collagen-solution-%e8%b2%a8%e8%99%9f-1250-01s-0-5-mg-ml-1ml%e5%8c%85%e8%a3%9d","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49682","title":{"rendered":"Human Type IV Collagen-Solution | \u8ca8\u865f 1250-01S \u00a0(0.5 mg\/mL) 1ml\u5305\u88dd"},"content":{"rendered":"

Human Type IV Collagen-Solution | \u8ca8\u865f 1250-01S \u00a0(0.5 mg\/mL) 1ml\u5305\u88dd<\/a><\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u81a0\u539f<\/strong>– Collagen<\/strong><\/span>\"\"<\/p>\n

\u95dc\u65bc\u81a0\u539f\uff08Collagen\uff09\uff1a<\/strong><\/span><\/p>\n

\u81a0\u539f\u86cb\u767d(Collagen)\u662f\u52d5\u7269\u7d50\u7de0\u7d44\u7e54\u91cd\u8981\u7684\u86cb\u767d\u8cea,\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\u7684\u542b\u91cf\u5c24\u70ba\u8c50\u5bcc\uff0c\u4f54\u6709\u6a5f\u9ad4\u86cb\u767d\u8cea\u767e\u5206\u4e4b25%-35%\u542b\u91cf\u3002\u81a0\u539f\u662f\u7d30\u80de\u5916\u6700\u91cd\u8981\u7684\u6c34\u4e0d\u6eb6\u6027\u7e96\u7dad\u86cb\u767d, \u662f\u69cb\u6210\u7d30\u80de\u5916\u57fa\u8cea\uff08extracellular matrix, ECM\uff09\u7684\u9aa8\u67b6\u3002\u81a0\u539f\u5728\u7d30\u80de\u5916\u57fa\u8cea\u4e2d\u5f62\u6210\u534a\u6676\u9ad4\u7684\u7e96\u7dad,\u7d66\u7d30\u80de\u63d0\u4f9b\u6297\u5f35\u529b\u548c\u5f48\u6027,\u4e26\u5728\u7d30\u80de\u7684\u9077\u79fb\u548c\u767c\u80b2\u4e2d\u8d77\u4f5c\u7528\u3002\u81a0\u539f\u86cb\u767d\u7684\u57fa\u672c\u7d50\u69cb\u55ae\u4f4d\u662f\u539f\u81a0\u539f(tropocollagen), \u539f\u81a0\u539f\u80bd\u93c8\u7684\u4e00\u7d1a\u7d50\u69cb\u5177\u6709(Gly-x-y)n\u91cd\u8907\u5e8f\u5217, \u5176\u4e2dx\u5e38\u70ba\u812f\u6c28\u9178(Pro), y\u5e38\u70ba\u7fa5\u812f\u6c28\u9178(Hypro)\u6216\u7fa5\u8cf4\u6c28\u9178(Hylys)\u3002Hylys\u6b98\u57fa\u53ef\u767c\u751f\u7cd6\u57fa\u5316\u4fee\u98fe,\u5176\u7cd6\u55ae\u4f4d\u6709\u7684\u662f\u4e00\u500b\u534a\u4e73\u7cd6\u6b98\u57fa(Gal), \u4f46\u901a\u5e38\u662f\u4e8c\u7cd6(Glu-Gal-),\u81a0\u539f\u4e0a\u7684\u7cd6\u6240\u5360\u7684\u91cf\u7d04\u70ba\u81a0\u539f\u768410%\u3002\u539f\u81a0\u539f\u662f\u7531\u4e09\u689d\u03b1-\u80bd\u93c8\u7d44\u6210\u7684\u7e96\u7dad\u72c0\u86cb\u767d\u8cea, \u76f8\u4e92\u64f0\u6210\u4e09\u80a1\u87ba\u65cb\u72c0\u69cb\u578b, \u9577300nm, \u76f4\u5f911.5nm\u3002\u76ee\u524d\u5df2\u767c\u73fe29\u7a2e\u4e0d\u540c\u7d44\u7e54\u4e2d\u4e0d\u540c\u985e\u578b\u7684\u81a0\u539f; \u4e0d\u540c\u985e\u578b\u7684\u81a0\u539f\u5b9a\u4f4d\u65bc\u9ad4\u5167\u7684\u7279\u5b9a\u7d44\u7e54,\u4e5f\u67092-3\u7a2e\u4e0d\u540c\u7684\u81a0\u539f\u5b58\u5728\u65bc\u540c\u4e00\u7d44\u7e54\u4e2d\u3002\u800c\u6a5f\u9ad4\u516790%\u7684\u81a0\u539f\u70baI\u3001II\u3001III\u3001IV\u578b\u3002<\/p>\n

\"SB\"<\/a><\/p>\n

 <\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong><\/span>\u81a0\u539f\u86cb\u767d(Collagen)\u662f\u7d30\u80de\u5916\u7a7a\u9593\u4e2d\u7684\u4e3b\u8981\u7d50\u69cb\u86cb\u767d\uff0c\u662fECM\u4e2d\u6700\u8c50\u5bcc\u7684\u86cb\u767d\u3002\u81a0\u539f\u86cb\u767d\u5206\u70ba\u5169\u985e-\u539f\u7e96\u7dad\uff08I\uff0cII\uff0cIII\uff0cV\u578b\uff09\u548c\u975e\u539f\u7e96\u7dad\uff08IV\uff0cVI\u578b\uff09\u3002 IV\u578b\u81a0\u539f\u86cb\u767d\u4e3b\u8981\u5728\u57fa\u5e95\u819c(basal lamina)\u4e2d\u8868\u9054\uff0c\u56e0\u6b64\u69cb\u6210\u4e86\u57fa\u5e95\u819c(basement membrane)\u7684\u4e00\u90e8\u5206\u3002 IV\u578b\u81a0\u539f\u86cb\u767d\u7a81\u8b8a\u8207Alport\u7d9c\u5408\u5fb5\u76f8\u95dc\uff0c\u800c\u03b13\uff08IV\uff09 \u93c8\u88ab\u8a8d\u70ba\u662fGoodpasture\u7d9c\u5408\u5fb5\u4e2d\u7684\u6297\u539f\u3002 IV\u578b\u81a0\u539f\u7531\u516d\u500b\u547d\u540d\u70ba\u03b11\uff08IV\uff09\u81f3\u03b16\uff08IV\uff09\u7684\u7570\u69cb\u93c8\u7d44\u6210\u3002SouthernBiotech\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u54c1\u8cea\u81a0\u539f-\u00a0 Collagen , \u5176\u4e2dHuman Type IV Collagen-Solution (\u8ca8\u865f<\/strong>1250-01S )\u4f86\u6e90\u70baPlacental villi, \u7d14\u5316\u65b9\u5f0f\u7d93\u7531\u63a7\u5236\u548c\u9650\u5236\u80c3\u86cb\u767d\u9176\u6d88\u5316\uff0c\u7136\u5f8c\u9032\u884c\u9078\u64c7\u6027\u9e7d\u6c89\u6fb1\u3002\u7d14\u5ea6> 90% by SDS-PAGE\uff0c\u8caf\u5b58\u5728500 mM acetic acid\u6eb6\u6db2\u4e2d\uff0c\u6fc3\u5ea6\u70ba0.5mg\/ml, \u9ad4\u7a4d1ml\u3002<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Description<\/strong><\/td>\nCollagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes – fibril (types I, II, III, V) and non-fibril (types IV, VI). Type IV collagen is primarily expressed in the basal lamina and therefore constitutes a portion of the basement membrane. Type IV collagen mutations are associated with Alport syndrome and the \u03b13(IV) chain is thought to be the antigen implicated in Goodpasture’s syndrome. Type IV collagen is comprised of six isomeric chains designated \u03b11(IV) to \u03b16(IV).<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPlacental villi<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n> 90% by SDS-PAGE<\/td>\n<\/tr>\n
Purification Method<\/strong><\/td>\nControlled and limited pepsin digestion followed by selective salt precipitation<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n500 mM acetic acid<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.5 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Recommended Storage<\/strong><\/td>\n2-8\u00b0C<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA \u2013 Quality tested\u00a02-4<\/sup>
\nSDS-PAGE \u2013 Quality tested\u00a01<\/sup>
\nCoating Material for \u2013
\nAdhesion Studies \u2013 Reported in literature\u00a05,6<\/sup>
\nBlood Disorder Studies \u2013 Reported in literature\u00a02-4<\/sup>
\nDifferentiation Studies \u2013 Reported in literature\u00a07<\/sup>
\nECM Interaction Studies \u2013 Reported in literature\u00a08,9<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

ELISA plate was coated with serially diluted Mouse Type IV Collagen (SB Cat. No. 1250-04S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u81a0\u539f\u86cb\u767d<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u7de8\u865f\uff08cat#\uff09<\/strong><\/td>\n\u5305\u88dd\u5f62\u5f0f<\/strong><\/td>\n\u898f\u683c<\/strong><\/td>\n<\/tr>\n
Type I Human Collagen<\/td>\n1200-01S<\/td>\n\u6eb6\u6db2<\/td>\n0.50mg<\/td>\n<\/tr>\n
Type I Bovine Collagen<\/td>\n1200-02\/1200-02S<\/td>\n\u51cd\u4e7e\u7c89\/\u6eb6\u6db2<\/td>\n0.25mg\/0.50mg<\/td>\n<\/tr>\n
Type I Rat Collagen<\/td>\n1200-03\/1200-03S<\/td>\n\u51cd\u4e7e\u7c89\/\u6eb6\u6db2<\/td>\n0.25mg\/0.50mg<\/td>\n<\/tr>\n
Type II Rat Collagen<\/td>\n1220-02\/1220-02S<\/td>\n\u51cd\u4e7e\u7c89\/\u6eb6\u6db2<\/td>\n0.25mg\/0.50mg<\/td>\n<\/tr>\n
Type III Human Collagen<\/td>\n1230-01S<\/td>\n\u6eb6\u6db2<\/td>\n0.50mg<\/td>\n<\/tr>\n
Type III Bovine Collagen<\/td>\n1240-02S<\/td>\n\u6eb6\u6db2<\/td>\n0.50mg<\/td>\n<\/tr>\n
Type IV Human Collagen<\/td>\n1250-01S<\/td>\n\u6eb6\u6db2<\/td>\n0.50mg<\/td>\n<\/tr>\n
Type IV Mouse Collagen<\/td>\n1250-04S<\/td>\n\u6eb6\u6db2<\/td>\n0.50mg<\/td>\n<\/tr>\n
Type IV Bovine Collagen<\/td>\n1260-02<\/td>\n\u51cd\u4e7e\u7c89<\/td>\n0.25mg<\/td>\n<\/tr>\n
Type V Human Collagen<\/td>\n1270-01\/1270-01S<\/td>\n\u51cd\u4e7e\u7c89\/\u6eb6\u6db2<\/td>\n0.1mg\/0.25mg<\/td>\n<\/tr>\n
Type V Bovine Collagen<\/td>\n1280-02S<\/td>\n\u6eb6\u6db2<\/td>\n0.25mg<\/td>\n<\/tr>\n
Type VI Human Collagen<\/td>\n1290-01S<\/td>\n\u6eb6\u6db2<\/td>\n0.25mg<\/td>\n<\/tr>\n
Type VI Bovine Collagen<\/td>\n1300-02\/1300-02S<\/td>\n\u51cd\u4e7e\u7c89\/\u6eb6\u6db2<\/td>\n0.10mg\/0.25mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u81a0\u539f\u86cb\u767d\u591a\u682a\u6297\u9ad4<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u7de8\u865f\uff08cat#\uff09<\/strong><\/td>\n\u5305\u88dd\u5f62\u5f0f<\/strong><\/td>\n\u898f\u683c<\/strong><\/td>\n<\/tr>\n
Goat Anti-Type I Collagen<\/td>\n1310-01\/1310-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n
Goat Anti-Type II Collagen<\/td>\n1320-01\/1320-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n
Goat Anti-Type III Collagen<\/td>\n1330-01\/1330-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n
Goat Anti-Type IV Collagen<\/td>\n1340-01\/1340-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n
Goat Anti-Type V Collagen<\/td>\n1350-01\/1350-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n
Goat Anti-Type VI Collagen<\/td>\n1360-01\/1360-08<\/td>\n\u672a\u6a19\u8a18\/\u751f\u7269\u7d20\u5316<\/td>\n0.2mg\/0.2mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u81a0\u539f\u86cb\u767d\u55ae\u682a\u6297\u9ad4<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n\u7de8\u865f\uff08cat#\uff09<\/strong><\/td>\n\u5305\u88dd\u5f62\u5f0f<\/strong><\/td>\n\u898f\u683c<\/strong><\/td>\n<\/tr>\n
Mouse Anti-Human Type I Collagen<\/td>\n1440(1)-01<\/td>\n\u672a\u6a19\u8a18<\/td>\n0.1mg<\/td>\n<\/tr>\n
Mouse Anti-Human Type IV Collagen<\/td>\n1460-01<\/td>\n\u672a\u6a19\u8a18<\/td>\n0.1mg<\/td>\n<\/tr>\n
Mouse Anti-Human Type IV Collagen<\/td>\n1460-02<\/td>\nFITC<\/td>\n0.1mg<\/td>\n<\/tr>\n
Mouse Anti-Human Type IV Collagen<\/td>\n1460-08<\/td>\nBIOT<\/td>\n0.1mg<\/td>\n<\/tr>\n
Mouse Anti-Human Type IV Collagen<\/td>\n1460-09<\/td>\nR-PE<\/td>\n0.1mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u61c9\u7528\uff1a<\/strong>\u00a0ELISA\u3001\u514d\u75ab\u87a2\u5149\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b78\uff08\u51b0\u51cd\u5207\u7247\uff09<\/span><\/p>\n

Isotype control\u00a0<\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a>\u00a0|\u00a0<\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a>\u00a0|\u00a0<\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA\u00a0<\/strong>\u5957\u7d44<\/strong><\/a>\u00a0|\u00a0<\/strong>anti-rabbit\u00a0<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a>\u00a0|\u00a0<\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a>\u00a0|<\/strong><\/span><\/p>\n

References<\/span><\/strong><\/span><\/p>\n

    \n
  1. Hou L, Coller J, Natu V, Hastie TJ, Huang NF. Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia. Acta Biomater. 2016;44:188-99. (Microarray, Cell Culture)<\/li>\n
  2. Slobodianuk TL, Kochelek C, Foeckler J, Kalloway S, Weiler H, Flood VH. Defective collagen binding and increased bleeding in a murine model of von Willebrand disease affecting collagen IV binding. J Thromb Haemost. 2019;17:63-71. (ELISA, Coating, Blood Disorder Studies, Adhesion Studies, Aggregation Studies)<\/li>\n<\/ol>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Human Type IV Collagen-Solution | \u8ca8\u865f 1250-01S \u00a0(0.5 mg\/ […]<\/p>\n","protected":false},"author":3,"featured_media":29305,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5381],"tags":[6637],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49682"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49682"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49682\/revisions"}],"predecessor-version":[{"id":49683,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49682\/revisions\/49683"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29305"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49682"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=49682"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=49682"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}