{"id":49536,"date":"2024-06-03T11:52:47","date_gmt":"2024-06-03T03:52:47","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49536"},"modified":"2025-11-18T14:00:35","modified_gmt":"2025-11-18T06:00:35","slug":"49536","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49536","title":{"rendered":"\u6d3b\u6b7b\u83cc\u67d3\u8272\u8a66\u5291\u76d2 | Live-Dead Bacterial Staining Kit APExBIO\u8ca8\u865fK2239"},"content":{"rendered":"

\u67d3\u8272\u7cfb\u5217\u5546\u54c1->>\u6d3b\u6b7b\u83cc\u67d3\u8272\u8a66\u5291\u76d2<\/span>
\n<\/span><\/span><\/span><\/strong><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291 (Small Molecule Inhibitors)\/\u6d3b\u5316\u5291(Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

\u6d3b\u6b7b\u83cc\u67d3\u8272\u8a66\u5291\u76d2 | Live-Dead Bacterial Staining Kit APExBIO\u8ca8\u865fK2239<\/strong><\/span><\/a><\/span><\/p>\n

A kit that can detect both live bacteria and dead bacteria at the same time<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong><\/span><\/p>\n

\u6d3b\u6b7b\u83cc\u67d3\u8272\u8a66\u5291\u76d2(The Live-Dead Bacterial Staining Kit )\u63d0\u4f9b\u540c\u6642\u6aa2\u6e2c\u6d3b\u83cc( live bacteria)\u548c\u6b7b\u83cc(dead bacteria)\u7684\u6aa2\u6e2c\u65b9\u6cd5\u3002\u8a66\u5291\u76d2\u5305\u542b\u5169\u7a2e\u67d3\u6599\uff1aNucGreen \u548c EthD-III\u3002 NucGreen\u662f\u4e00\u7a2e\u7da0\u8272\u6838\u9178\u67d3\u6599(green nucleic acid dye)\uff0c\u53ef\u5c0d\u6d3b\u83cc\u548c\u6b7b\u7d30\u83cc\u9032\u884c\u67d3\u8272\uff1b EthD-III\u662f\u4e00\u7a2e\u7d05\u8272\u6838\u9178\u67d3\u6599(red nucleic acid dye)\uff0c\u50c5\u5c0d\u7d30\u80de\u819c\u53d7\u640d(damaged cell membranes)\u7684\u7d30\u83cc\uff08\u4f8b\u5982\u6b7b\u7d30\u83cc\uff09\u9032\u884c\u67d3\u8272\u3002\u900f\u904e\u6b64\u8a66\u5291\u76d2\u53ef\u4ee5\u8b58\u5225\u7d30\u83cc\u7684\u6d3b\u529b\uff0c\u5065\u5eb7\u7684\u7d30\u83cc\u5448\u73fe\u7da0\u8272\uff0c\u6b7b\u4ea1\u7684\u7d30\u83cc\u5448\u73fe\u7da0\u8272\u548c\u7d05\u8272\u758a\u52a0\u3002<\/p>\n

<\/p>\n\n\n\n\n\n
Components<\/b><\/td>\nK2239-20 T<\/b><\/td>\nK2239-100 T<\/b><\/td>\n<\/tr>\n
NucGreen<\/b><\/td>\n20 \u03bcL<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
EthD-III<\/b><\/td>\n40 \u03bcL<\/td>\n200 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

1. \u7d30\u83cc\u6a23\u54c1\u88fd\u5099\uff1a<\/span><\/strong><\/span><\/p>\n

1) \u5c07\u7d30\u83cc\u5728\u9069\u7576\u7684\u6db2\u9ad4\u57f9\u990a\u57fa\u4e2d\u57f9\u990a\u81f3\u5c0d\u6578\u751f\u9577\u5f8c\u671f\u3002
\n2) \u53d61 mL\u83cc\u6db2\uff0c5000-10000 g\u96e2\u5fc310-15 min\u3002
\n3) \u9664\u53bb\u4e0a\u6e05\u6db2\uff0c\u52a0\u5165 1 mL 0.85% NaCl \u6eb6\u6db2\u81f3\u7ba1\u5167\u91cd\u61f8\u7d30\u83cc\u30025000-10000 g \u96e2\u5fc3 10-15 \u5206\u9418\uff0c\u9664\u53bb\u4e0a\u6e05\u6db2\u3002\u52a0\u5165 1 mL 0.85% \u6c2f\u5316\u9209\u6eb6\u6db2\u91cd\u61f8\u7d30\u83cc\u4e26\u91cd\u8907\u96e2\u5fc3\u4e00\u6b21\u3002
\n4) \u4ee50.85% NaCl\u6eb6\u6db2\u91cd\u61f8\u7d30\u83cc\uff0c\u8abf\u6574\u7d30\u80de\u5bc6\u5ea6\u81f3108\u500b\u7d30\u83cc\/mL\uff08OD670~0.03)<\/p>\n

2.\u7d30\u83cc\u67d3\u8272\uff1a<\/span><\/strong><\/p>\n

1) \u53d6 1 \u03bcL NucGreen\u30012 \u03bcL EthD-III\u30013 \u03bcL 0.85% NaCl \u6eb6\u6db2\uff0c\u5145\u5206\u6df7\u52fb\uff0c\u88fd\u6210\u7d30\u83cc\u67d3\u8272\u5de5\u4f5c\u6db2\u3002
\n2) \u5c075 \u03bcL\u67d3\u8272\u5de5\u4f5c\u6db2\u52a0\u5165100 \u03bcL\u83cc\u6db2\u4e2d\uff0c\u6df7\u52fb\uff0c\u5b75\u80b2\u5ba4\u6eab\u907f\u5149 15 \u5206\u9418\u3002<\/p>\n

\u6aa2\u6e2c\uff1a\u67d3\u8272\u5f8c\uff0c\u5728\u4e7e\u6de8\u7684\u73bb\u7247\u4e0a\u52a0\u516510\u03bcL\u83cc\u6db2\uff0c\u84cb\u4e0a\u73bb\u7483\u84cb\u73bb\u7247\u3002\u5169\u7a2e\u67d3\u6599\u7684\u6aa2\u6e2c\u689d\u4ef6\u70ba\uff1a NucGreen\uff1aEx\/Em\uff1a503\/530 nm\uff08\u8207 DNA \u7d50\u5408\u5f8c\uff09 EthD-III\uff1aEx\/Em\uff1a530\/620 nmmm\uff08\uff08\u8207 DNA \u7d50\u5408\u5f8c\uff09\u6700\u4f73\u5316\uff1b\u6839\u64da\u4e0d\u540c\u83cc\u682a\u9069\u7576\u8abf\u6574\u67d3\u6599\u6fc3\u5ea6\uff0c\u4e00\u822c\u76e1\u91cf\u4f7f\u7528\u80fd\u63d0\u4f9b\u8db3\u5920\u8a0a\u865f\u7684\u6700\u4f4e\u67d3\u6599\u6fc3\u5ea6\u3002\u5408\u4f75\u6642\u5efa\u8b70\u4fdd\u6301\u7d05\u7da0\u87a2\u5149\u4eae\u5ea6\u4e00\u81f4\u3002\u975c\u7f6e10\u5206\u9418\u5f8c\u53ef\u7559\u4e0b\u5c11\u91cf\u83cc\u6db2\u9032\u884c\u6210\u50cf\uff0c\u53ef\u6709\u6548\u964d\u4f4e\u80cc\u666f\u3002<\/p>\n

\"\"\u5316\u5b78\u67d3\u8272\u8a66\u5291<\/span><\/strong><\/span><\/p>\n