{"id":49328,"date":"2024-04-28T17:19:35","date_gmt":"2024-04-28T09:19:35","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=49328"},"modified":"2025-11-15T15:42:38","modified_gmt":"2025-11-15T07:42:38","slug":"%e4%b8%ad%e6%80%a7%e7%b4%85%e8%a1%80%e7%90%83%e5%a2%9e%e6%ae%96%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-neutral-red-cell-proliferation-assay-kit-apexbio%e8%b2%a8%e8%99%9f-k2262","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=49328","title":{"rendered":"\u4e2d\u6027\u7d05\u8840\u7403\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2| Neutral Red Cell Proliferation Assay Kit APExBIO\u8ca8\u865f K2262"},"content":{"rendered":"

\u4e2d\u6027\u7d05\u8840\u7403\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2| Neutral Red Cell Proliferation Assay Kit APExBIO\u8ca8\u865f K2262<\/span><\/strong><\/span><\/a><\/span><\/p>\n

A kit to detect cell proliferation by neutral red<\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong><\/span><\/p>\n

\u4e2d\u6027\u7d05\u8840\u7403\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2(Neutral Red Cell Proliferation Assay Kit )\u662f\u4e00\u7a2e\u53ef\u4ee5\u5feb\u901f\u6aa2\u6e2c\u7d30\u80de\u589e\u6b96(cell proliferation )\u6216\u6bd2\u6027(toxicity)\u7684\u8a66\u5291\u76d2\u3002\u7d30\u80de\u5c0d\u4e2d\u6027\u7d05(neutral red )\u7684\u5438\u6536\u53d6\u6c7a\u65bc\u7d30\u80de\u7dad\u6301 pH \u68af\u5ea6\u7684\u80fd\u529b\uff0c\u800c pH \u68af\u5ea6\u53c8\u53d6\u6c7a\u65bc ATP \u7684\u7522\u751f(ATP production)\u3002\u5728\u751f\u7406pH\u689d\u4ef6\u4e0b( physiological pH conditions)\uff0c\u4e2d\u6027\u7d05(neutral red )\u53ef\u85c9\u7531\u88ab\u52d5\u64f4\u6563(passive diffusion )\u9032\u5165\u7d30\u80de\u819c\u4e26\u5728\u6eb6\u5c0f\u9ad4(lysosomes)\u4e2d\u7d2f\u7a4d\u3002\u7576\u7d30\u80de\u6578\u91cf\u589e\u52a0\u6642\uff0c\u4e2d\u6027\u7d05(neutral red )\u7684\u651d\u53d6\u91cf\u4e5f\u6703\u589e\u52a0\u3002\u76f8\u53cd\uff0c\u7576\u7d30\u80de\u53d7\u640d\u6642\uff0c\u5b83\u5011\u651d\u53d6\u4e2d\u6027\u7d05(neutral red )\u7684\u80fd\u529b\u5c31\u6703\u6e1b\u5f31\u3002\u900f\u904e\u6e2c\u91cf\u7d30\u80de\u651d\u53d6\u7684\u4e2d\u6027\u7d05(neutral red )\u7684\u91cf\uff0c\u53ef\u4ee5\u8a55\u4f30\u7d30\u80de\u7684\u589e\u6b96\u6216\u6bd2\u6027\u3002\u6b64\u5916\uff0c\u4e2d\u6027\u7d05(neutral red )\u8272\u4e5f\u53ef\u7528\u4f5cpH\u6307\u793a\u5291\uff0c\u5176\u984f\u8272\u6703\u96a8pH\u503c\u800c\u8b8a\u5316\u3002\u4e2d\u6027\u7d05(neutral red )\u5728\u9178\u6027\u74b0\u5883\u4e2d\u5448\u73fe\u7d05\u8272\uff0c\u7576pH\u503c\u5f9e6.8\u4e0a\u5347\u81f38.0\u6642\uff0c\u984f\u8272\u9010\u6f38\u7531\u7d05\u8272\u8b8a\u6210\u9ec3\u8272\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

1. Ready-to-use lysis buffer preparation:<\/p>\n

Add 50 mL of absolute ethanol to the bottle of Neutral Red Lysis Buffer and shake to make the ready-to-use lysis buffer.<\/p>\n

2. Cell culture:<\/p>\n

For 96-well plates, seed cells at a density of 5-100×103 cells\/well in 100 \u03bcL culture medium.Treat cells with the interested drug for a desired period. Prepare parallel wells as the background control (onlycontaining medium) and negative control (containing medium, cells and the same volume of solvent for theinterested drug)<\/p>\n

3. Neutral red intake:<\/p>\n

Prepare the neutral red staining solution referring to the table below. Remove the medium, add the freshly prepared neutral red staining solution, and incubate in the incubator for 2 h. The
\nincubation time can be adjusted according to the specific experiment and can be extended to 3-4 h.<\/p>\n

<\/p>\n

4. Neutral red lysis:<\/p>\n

Remove medium and wash 1-2 times with PBS. Add 200 \u03bcL of ready-to-use lysis buffer and lyse on a shaker at room temperature for 10 minutes.<\/p>\n

5. Detection:<\/p>\n

Measure the absorbance (A) at 540 nm with a microplate reader. A wavelength of 690 nm can be selected as the reference wavelength.<\/p>\n

6. Analysis: Calculate cell viability with the following equation
\nCell viability (%) =\u02f7\u02c4A Treatment sample – A Background control\u02c5\/\u02c4A Negative control – A Background control\u02c5\u02f9x 100<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n

https:\/\/www.apexbt.com<\/a><\/span><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9\u6578<\/span><\/td>\n\u7522\u54c1\u4ecb\u7d39\u9023\u7d50<\/span><\/td>\n<\/tr>\n
K2249<\/span><\/td>\nMTT\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/td>\n500T\/2500T<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/span><\/a><\/span><\/td>\n<\/tr>\n
K2262<\/strong><\/a><\/span><\/td>\n\u4e2d\u6027\u7d05\u8840\u7403\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong><\/a><\/span><\/td>\n500T<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/span><\/span><\/a><\/span><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n\u64cd\u4f5c\u624b\u518a<\/span><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n\u64cd\u4f5c\u624b\u518a<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e2d\u6027\u7d05\u8840\u7403\u589e\u6b96\u6aa2\u6e2c\u8a66\u5291\u76d2| Neutral Red Cell Proliferation Assay Kit […]<\/p>\n","protected":false},"author":3,"featured_media":45049,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[6727],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49328"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=49328"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49328\/revisions"}],"predecessor-version":[{"id":53746,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/49328\/revisions\/53746"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/45049"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=49328"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=49328"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=49328"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}