{"id":48403,"date":"2024-01-24T15:17:58","date_gmt":"2024-01-24T07:17:58","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=48403"},"modified":"2025-03-21T14:03:22","modified_gmt":"2025-03-21T06:03:22","slug":"%e6%b0%b8%e7%94%9f%e5%8c%96%e4%ba%ba%e9%a1%9e%e5%bf%83%e8%87%9f%e7%ba%96%e7%b6%ad%e6%af%8d%e7%b4%b0%e8%83%9ebma3-1a7-abmgood%e8%b2%a8%e8%99%9ft0446","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=48403","title":{"rendered":"\u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96\u7dad\u6bcd\u7d30\u80de | ABMGOOD\u8ca8\u865fT0446"},"content":{"rendered":"

A<\/strong>bmgood\u4e0d\u673d<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong> (immortalized cell)-\u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96\u7dad\u6bcd\u7d30\u80de<\/span><\/strong><\/span><\/p>\n

\u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96\u7dad\u6bcd\u7d30\u80de (\u8ca8\u865fT0446)<\/span><\/a><\/span><\/strong><\/p>\n

Immortalized Human Cardiac Fibroblasts<\/p>\n

<\/p>\n

\u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96\u7dad\u6bcd\u7d30\u80de\u7279\u6027<\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u578b\u614b: Adherent, multipolar\u3002 \u4f86\u6e90Heart\u3002\u89e3\u51cd\u5f8c\uff0c\u7d30\u80de\u9700\u89814-5\u5929\u624d\u80fd\u6062\u5fa9\u57f9\u990a<\/span>\u3002 \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u4f7f\u7528PriCoat\u2122T25\u57f9\u990a\u74f6 (\u8ca8\u865fG299<\/a><\/span>)\uff0c\u4ee5\u6700\u4f73\u5316\u521d\u4ee3\u548c\u4e0d\u673d\u7d30\u80de\u7684\u751f\u9577\u3001\u589e\u6b96\u548c\u64f4\u589e\u3002\u6216\u5857\u5c64\u7d30\u80de\u5916\u57fa\u8cea(Applied Cell Extracellular Matrix) \u589e\u5f37\u7d30\u80de\u8cbc\u9644 (\u8ca8\u865fG422<\/a><\/span>)\u3002Grow cells in ECM-coated culture vessels with the following conditions. \u57f9\u990a\u6db2PriGrow IV (\u8ca8\u865fTM004<\/a><\/u>)\u3002+ 10% FBS + 1 mM NEAA (TM068<\/a>) + 2 mM L-glutamine (G275<\/a>)+ \u00a05 \u00b5g\/ml insulin (TM053<\/a>) + 1% Penicillin\/Streptomycin Solution (G255<\/a>) \u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002<\/p>\n

\u00a0\"\"<\/div>\n
\n
Specifications<\/span><\/strong><\/span><\/div>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT0446<\/span><\/td>\n<\/tr>\n
Name<\/td>\nImmortalized Human Cardiac Fibroblasts<\/td>\n<\/tr>\n
Description<\/td>\nHuman Cardiac Fibroblast is an important heart component that provides structural support for cardiomyoctes. They synthesize extracellular matrix, growth factors and cytokines during growth. In addition, they also interact with myocardial responses to injury and pathophysiological conditions such as scar formation at sites of myocardial infarction, cardiac fibrosis and cardiac hypertrophy. These cells are ideal models for study in cardiac matrix response modeled by physiological and pathological stressors. Real Time PCR was used to quantify the SV-40 transgene expression.<\/td>\n<\/tr>\n
Organism<\/td>\nHuman (H. sapiens)<\/td>\n<\/tr>\n
Tissue<\/td>\nHeart<\/td>\n<\/tr>\n
Donor History<\/td>\nNot disclosed, Ventricle<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent, multipolar<\/td>\n<\/tr>\n
Cell Type<\/td>\nImmortalized Cells<\/td>\n<\/tr>\n
Storage Condition<\/td>\nVapor phase of liquid nitrogen, or below -130\u00b0C.<\/td>\n<\/tr>\n
Shipping Conditions<\/td>\nShip with dry ice.<\/td>\n<\/tr>\n
Product Format<\/td>\nFrozen<\/td>\n<\/tr>\n
Intended Use<\/td>\nThis product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.<\/td>\n<\/tr>\n
BioSafety<\/td>\nII<\/td>\n<\/tr>\n
Certificate of Analysis<\/td>\nFor batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.<\/td>\n<\/tr>\n
Growth Conditions<\/td>\nPriCoat\u2122 T25 Flasks (G299<\/a>) or Applied Cell Extracellular Matrix (G422<\/a>) are recommended for optimal cell culture.<\/strong>\u00a0PriGrow IV (TM004<\/a>) + 10% FBS + 1 mM NEAA (TM068<\/a>) + 2 mM L-glutamine (G275<\/a>)+ \u00a05 \u00b5g\/ml insulin (TM053<\/a>) + 1% Penicillin\/Streptomycin Solution (G255<\/a>), 37.0\u00b0C, 5% CO\u2082.<\/td>\n<\/tr>\n
Unpacking and Storage Instructions<\/td>\n\n

1. Visually examine the packaging containers for signs of leakage or breakage.<\/p>\n

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u00b0C, preferably in liquid nitrogen vapor phase storage, until ready for use.<\/p>\n

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u00b0C or in liquid nitrogen vapor phase. Do not store at -70\u00b0C, as it will result in loss of viability.<\/td>\n<\/tr>\n

Thawing Protocol<\/td>\n\n

1. Thaw cells quickly in a 37\u00b0C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.<\/p>\n

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.<\/p>\n

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.<\/p>\n

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.<\/p>\n

5. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n

Subculture Protocol<\/td>\n\n

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.<\/p>\n

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.<\/p>\n

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u00b0C, for several minutes to facilitate detachment.<\/p>\n

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.<\/p>\n

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.<\/p>\n

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.<\/p>\n

6. Incubate the cells at the recommended conditions.<\/td>\n<\/tr>\n

Cryopreservation<\/td>\nCryopreservation Medium (TM024), or complete growth media with 10% DMSO.<\/td>\n<\/tr>\n
Seeding Density (cells\/cm2<\/sup>)<\/td>\n40,000 – 50,000<\/td>\n<\/tr>\n
Population Doubling Time (h)<\/td>\n48 – 58<\/td>\n<\/tr>\n
Immortalization Method<\/td>\nSerial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen<\/td>\n<\/tr>\n
Expression<\/td>\nvWF and caveolin-1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

<\/p>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/a><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n

\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u00a0(immortalized reagenet)\u00a0Ready-to-use<\/span><\/strong><\/span><\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002<\/p>\n

\"cell-biology-tab-final<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Decomplement<\/strong>\u52a0\u71b1\u975e\u6d3b\u5316FBS\uff1a<\/strong><\/span><\/p>\n

1.\u8b93FBS\u74f6\u57284\u00b0C\u4e0b\u5b8c\u5168\u89e3\u51cd\u904e\u591c\u3002<\/p>\n

2.\u5c07FBS\u74f6\u7f6e\u65bc56\u00b0C\u7684\u6c34\u6d74\u4e2d30\u5206\u9418\u3002<\/p>\n

Decomplemented FBS\u53ef\u4ee5\u5728-20\u00b0C\u4e0b\u9577\u671f\u4fdd\u5b58\u3002\u907f\u514d\u983b\u7e41\u7684\u51cd\u878d\u5faa\u74b0\u3002\u6bcf2-3\u5929\u66f4\u63db\u4e00\u6b21\u5b8c\u6574\u7684\u5a92\u9ad4\u3002\u4e0d\u8981\u8b93\u4ecb\u8cea\u984f\u8272\u8b8a\u70ba\u9ec3\u8272\u3002<\/p>\n

Thawing<\/strong>\u7d30\u80de\u89e3\u51cd\u65b9\u6cd5\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u572837\u2103\u6c34\u6d74\u4e2d\u89e3\u51cd\u5c0f\u74f6\uff0c\u76f4\u5230\u53ea\u6709\u4e00\u5c0f\u584a\u51b0\u584a\u3002(DMSO\u5c0d\u7d30\u80de\u6709\u6bd2\u6027\uff0cover-thaw\u904e\u5ea6\u89e3\u51cd\u4e0b\u53ef\u80fd\u9020Stress)<\/span><\/p>\n

2.\u5c07\u7d30\u80de\u8f49\u79fb\u5230\u542b\u67095ml pre-warmed complete media \u4e4b15ml\u7ba1\u4e2d\u3002<\/p>\n

3.\u5c07\u7d30\u80de\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

4.\u5c0f\u5fc3\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u4e0d\u8981\u6253\u64fe\u7d30\u80de\u6c89\u6fb1\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u57281ml\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

5.\u5c07\u7d30\u80de\u57f9\u990a\u572850,000 – 100,000 cells\/cm2<\/sup>\u3002\u00a0cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5<\/p>\n

\"\"<\/p>\n

DMSO\u5728\u7d30\u80de\u51cd\u5b58\u4e2d\u7684\u61c9\u7528<\/span><\/strong><\/p>\n

\u4e8c\u7532\u57fa\u4e9e\u78b8\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u6ef2\u900f\u578b\u7d30\u80de\u4fdd\u8b77\u5291\u3002\u5728\u6df1\u4f4e\u6eab\uff08\u96f6\u4e0b200\u5ea6\uff09\u4fdd\u5b58\u7d30\u80de\u6642\u51cd\u5b58\u904e\u7a0b\u4e2d\uff0c\u70ba\u9632\u6b62\u7d30\u80de\u5167\u6db2\u51b0\u6676\u5f62\u6210\u3001\u6ef2\u900f\u58d3\u6539\u8b8a\u3001\u7d30\u80de\u7d50\u69cb\u7d0a\u4e82\u7b49\u5c0e\u81f4\u7684\u640d\u50b7\uff0c\u6709\u5fc5\u8981\u4f7f\u7528\u542b\u6709DMSO\u51b7\u51cd\u4fdd\u8b77\u5291\u3002DMSO\u80fd\u5920\u5feb\u901f\u7a7f\u900f\u7d30\u80de\u819c\u9032\u5165\u7d30\u80de\u4e2d\uff0c\u964d\u4f4e\u51b0\u9ede\u3001\u5ef6\u7de9\u51cd\u5b58\u904e\u7a0b\uff0c\u540c\u6642\u63d0\u9ad8\u7d30\u80de\u5167\u96e2\u5b50\u6fc3\u5ea6\uff0c\u6e1b\u5c11\u7d30\u80de\u5167\u51b0\u6676\u7684\u5f62\u6210\uff0c\u5f9e\u800c\u6e1b\u5c11\u7d30\u80de\u640d\u50b7\u3002\u6df1\u4f4e\u6eab\u6642\u4e8c\u7532\u57fa\u4e9e\u78b8\u7684\u7d30\u80de\u6bd2\u6027\u53d7\u5230\u6291\u5236\u3002\u5fa9\u8607\u6642\u52d5\u4f5c\u8981\u5feb\uff0c\u5118\u5feb\u6d17\u6389\u4e8c\u7532\u57fa\u4e9e\u78b8\uff0c\u5426\u5247\u6703\u9020\u6210\u5c0d\u7d30\u80de\u56b4\u91cd\u7684\u6bd2\u6027\u3002\u4e8c\u7532\u57fa\u4e9e\u78b8\uff08DMSO\uff09\u662f\u76ee\u524d\u6700\u597d\u7684\u7d30\u80de\u51cd\u5b58\u4fdd\u8b77\u5291\uff0c\u4f46\u4e5f\u662f\u4e00\u7a2e\u4ee5\u7d30\u80de\u6bd2\u6027\u5f88\u5927\u7684\u5316\u5b78\u8a66\u9a57\u5291\u3002\u7814\u7a76\u7d50\u679c\u8868\u660e\uff0c\u57f9\u990a\u6db2\u4e2dDMSO\u6fc3\u5ea6\u70ba10%\u6642\uff0c\u7d30\u80de\u751f\u9577\u6291\u5236\u7387\u8fd1100%\uff1b1\u2030\u6fc3\u5ea6\u6642\u6291\u5236\u7387\u70ba35%\uff0c\u5373\u4f7f\u662f0.04\u2030\u7684\u6fc3\u5ea6\uff0cDMSO\u5c0d\u7d30\u80de\u7684\u751f\u9577\u4e5f\u6709\u4e0d\u5229\u7684\u5f71\u97ff\u3002<\/p>\n

Subculture<\/strong>\u7d30\u80de\u7e7c\u4ee3\u65b9\u6cd5:<\/strong>\u00a0(<\/strong>\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)<\/strong>\u00a0\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/strong><\/a><\/span><\/span><\/p>\n

1.\u7576\u7d30\u80de70-90\uff05 confluency\u6642\uff0c\u5efa\u8b70\u7e7c\u4ee3\u57f9\u990a\u3002<\/p>\n

2. Aspirate old media\u3002\u61f8\u6d6e\u6db2\u4e2d\u7684\u4e00\u4e9b\u7d30\u80de\u4ecd\u7136\u662f\u6d3b\u7d30\u80de\u3002\u6216\u8005\uff0c\u4e5f\u53ef\u4ee5\u6536\u96c6\u542b\u6709\u61f8\u6d6e\u7d30\u80de\u7684\u4e0a\u6e05\u6db2\u4e26\u96e2\u5fc3\uff08Skip to Step 6 in protocol\uff09\u3002<\/p>\n

3.\u52a0\u51651\uff1a1\u6bd4\u4f8b\u76841X\u7121\u83ccPBS\u548c0.25\uff05\u80f0\u86cb\u767d\u9176-EDTA\uff08TM051\uff09\u3002<\/p>\n

4.\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u7d30\u80de3-5\u5206\u9418\u4e26\u652a\u62cc\u57f9\u990a\u5bb9\u5668\u76f4\u81f390\uff05\u7684\u7d30\u80de\u812b\u843d\u3002<\/p>\n

5. \u4e2d\u548c\u80f0\u86cb\u767d\u9176:\u6dfb\u52a0\u7b49\u91cf\u4e4btrypsin + PBS\u3002<\/p>\n

6.\u6536\u96c6\u7d30\u80de\u4e26\u4ee5290xg\u96e2\u5fc35\u5206\u9418\u3002<\/p>\n

7.\u4e1f\u68c4\u4e0a\u6e05\u6db2\uff0c\u8f15\u8f15\u5730\u4e0a\u4e0b\u79fb\u6db2\uff0c\u5c07\u7d30\u80de\u91cd\u65b0\u61f8\u6d6e\u5728\u5b8c\u5168\u57f9\u990a\u57fa\u4e2d\u3002<\/p>\n

8.\u5efa\u8b70split ratio\u6bd4\u4e0d\u8d85\u904e1\uff1a3\u3002<\/p>\n

Freezing<\/strong>\u7d30\u80de\u51cd\u7d50\uff1a(\u6bcf\u7a2e\u7d30\u80de\u5efa\u8b70\u689d\u4ef6\u53ef\u80fd\u6709\u8a31\u4e0d\u540c\uff0c\u4f9d\u7167\u539f\u5ee0\u5efa\u8b70\u64cd\u4f5c\uff0c\u4ee5\u4e0b\u70ba\u901a\u7528\u65b9\u5f0f)\u00a0<\/strong>\u64cd\u4f5c\u65b9\u5f0f\u4e0b\u8f09<\/a><\/strong><\/span><\/span><\/p>\n

Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.<\/p>\n

\u5132\u5b58\u6eab\u5ea6\uff1a\u6db2\u6c2e\u6c23\u76f8\u3002\u91cd\u8981\u63d0\u793a\uff1a\u6b64\u7d30\u80de\u4fc2\u5c0dFBS\u6279\u6b21\u975e\u5e38\u654f\u611f\u3002\u6211\u5011\u5f37\u70c8\u5efa\u8b70\u6700\u7d42\u7528\u6236\u4f7f\u7528\u5f9ePAN biotech\u6216Nucleus Biologics \u4e4bFBS\u3002<\/p>\n

\u6162\u75c5\u6bd2\u5305\u88dd\u7cfb\u5217\u7522\u54c1Lentivirus<\/u>\u6162\u75c5\u6bd2\u4f4e\u901f\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus\u00a0<\/u>\u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316<\/u>(\u7d14\u5316)\u00a0<\/a>|\u00a0Lentivirus<\/u>\u75c5\u6bd2RT-QPCR<\/u>\u5b9a\u91cf<\/u>(\u5b9a\u91cf)<\/a>\u00a0<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat No<\/strong><\/td>\nProduct Name<\/strong><\/td>\n<\/tr>\n
T0016<\/td>\nImmortalized Human Cord Blood Mesenchymal Stromal Cells (cbMSC-hTERT)<\/td>\n<\/tr>\n
T0017<\/td>\nImmortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP)<\/td>\n<\/tr>\n
T0533<\/td>\nImmortalized Human Endometrial Stromal Cells (HESC)<\/td>\n<\/tr>\n
T0697<\/td>\nImmortalized Human Cranial Suture Progenitor Cells (iSuPs)<\/td>\n<\/tr>\n
T0723<\/td>\nImmortalized Human Foreskin Keratinocytes (HFK-16E6E7)<\/td>\n<\/tr>\n
T0727<\/td>\nImmortalized Human Prostate Stromal Cells (PM151T) – hTERT<\/td>\n<\/tr>\n
T0752<\/td>\nImmortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5)<\/td>\n<\/tr>\n
T0764<\/td>\nImmortalized Human Endometriotic Cell Line (12Z)<\/td>\n<\/tr>\n
T0841<\/td>\nImmortalized Human Dental Pulp Stem Cells (TP-023)<\/td>\n<\/tr>\n
T0842<\/td>\nImmortalized Human Dental Pulp Stem Cells (TP-037)<\/td>\n<\/tr>\n
T1013<\/td>\nImmortalized Human Diploid Fibroblasts – hTERT<\/td>\n<\/tr>\n
T1015<\/td>\nImmortalized Human Lung Fibroblast – hTERT<\/td>\n<\/tr>\n
T9435<\/td>\nImmortalized Human Cancer Associated Fibroblast Cells – SV40<\/td>\n<\/tr>\n
T3000<\/td>\nAcan CRISPR Knockout RCS Cell Line (Rat)<\/td>\n<\/tr>\n
T3002<\/td>\nAquaporin-4 Stable 293 Cell Line<\/td>\n<\/tr>\n
T3005<\/td>\nMuSK Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3006<\/td>\nLuciferase Stable PC3 Cell Line<\/td>\n<\/tr>\n
T3008<\/td>\nLuciferase (Luc2) Stable TRAMP Cell Line<\/td>\n<\/tr>\n
T3009<\/td>\nLuciferase Stable U87MG\/\u0394EGFR Cell Line<\/td>\n<\/tr>\n
T3013<\/td>\nLuciferase Stable WM-266-4 Cell Line<\/td>\n<\/tr>\n
T3015<\/td>\nNF-kB Luciferase Stable RAW264.7 Cell Line<\/td>\n<\/tr>\n
T3016<\/td>\nHEK293-CymR-Puro Stable Cell Line<\/td>\n<\/tr>\n
T3017<\/td>\nA2058-CymR Stable Cell Line<\/td>\n<\/tr>\n
T3018<\/td>\nSKMEL5-CymR Stable Cell Line<\/td>\n<\/tr>\n
T3021<\/td>\nGRPR-Luciferase Stable PC3 Cell Line<\/td>\n<\/tr>\n
T3024<\/td>\nBax Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3025<\/td>\nBak Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3026<\/td>\nBax\/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3027<\/td>\nAtg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3028<\/td>\nAtg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line<\/td>\n<\/tr>\n
T3030<\/td>\nNav2.1\/&beta;1\/Contactin Stable Chinese Hamster Lung Cell Line<\/td>\n<\/tr>\n
T3031<\/td>\nPARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line<\/td>\n<\/tr>\n
T3032<\/td>\nDual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line<\/td>\n<\/tr>\n
T3033<\/td>\nDual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4&lt;sup&gt;esn&lt;\/sup&gt;) Cell Line<\/td>\n<\/tr>\n
T3034<\/td>\nTLR3 Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3035<\/td>\nTLR9 Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3036<\/td>\nType I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line<\/td>\n<\/tr>\n
T3037<\/td>\nRyanodine Receptor 1 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3038<\/td>\nKCC2 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3039<\/td>\nStable HEK293 Expressing Mutant UQCRB-MT1 Cell Line<\/td>\n<\/tr>\n
T3040<\/td>\nc-Kit\/D816V Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3041<\/td>\nc-Kit\/WT Stable Ba\/F3 (with GNNK- isoform) Cell Line<\/td>\n<\/tr>\n
T3042<\/td>\nc-Kit\/WT Stable Ba\/F3 (with GNNK+ isoform) Cell Line<\/td>\n<\/tr>\n
T3043<\/td>\nc-Kit\/Y568F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3044<\/td>\nc-Kit\/Y570F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3045<\/td>\nc-Kit Y568F\/Y570F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3046<\/td>\nc-Kit\/Y703F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3047<\/td>\nc-Kit\/Y936F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3048<\/td>\nc-Kit Y703F\/Y936F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3049<\/td>\nc-Kit\/N705AStable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3050<\/td>\nc-Kit\/N938A Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3051<\/td>\nc-Kit N705A\/N938A Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3052<\/td>\nc-Kit\/D816H Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3053<\/td>\nc-Kit\/D816F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3054<\/td>\nc-Kit\/D816YStable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3055<\/td>\nc-Kit\/V560D Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3056<\/td>\nc-Kit\/K642E Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3057<\/td>\nc-Kit\/Y721F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3058<\/td>\nc-Kit\/Y823F Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3059<\/td>\nTLR2 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3060<\/td>\nPGC-1\u03b1\/ERR\u03b1 Stable 293T Cell Line<\/td>\n<\/tr>\n
T3061<\/td>\nTNK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3062<\/td>\nALK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3063<\/td>\nLTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3064<\/td>\nAXL Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3065<\/td>\nMERTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3066<\/td>\nTYRO3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3067<\/td>\nEPHA1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3068<\/td>\nEPHA5 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3069<\/td>\nEPHA7 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3070<\/td>\nEPHA8 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3071<\/td>\nFGFR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3072<\/td>\nFGFR3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3073<\/td>\nIGF1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3074<\/td>\nINSR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3075<\/td>\nJAK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3076<\/td>\nJAK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3077<\/td>\nJAK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3078<\/td>\nMET Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3079<\/td>\nMST1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3080<\/td>\nFLT3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3081<\/td>\nKIT Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3082<\/td>\nPDGFRA Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3083<\/td>\nPDGFRB Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3084<\/td>\nRET Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3085<\/td>\nROS1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3086<\/td>\nBLK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3087<\/td>\nFGR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3088<\/td>\nFYN Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3089<\/td>\nHCK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3090<\/td>\nLCK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3091<\/td>\nSRC Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3092<\/td>\nYES1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3093<\/td>\nSYK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3094<\/td>\nNTRK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3095<\/td>\nNTRK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3096<\/td>\nNTRK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3097<\/td>\nFLT4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3098<\/td>\nERBB2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3099<\/td>\nEPHA4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3100<\/td>\nABL1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3101<\/td>\nABL2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3102<\/td>\nAZ-AHR Stable HepG2 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3103<\/td>\nAZ-GR Stable HeLa Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3104<\/td>\nAIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3105<\/td>\nBMP Responsive Immortalized Reporter (BRITER) Cell Line<\/td>\n<\/tr>\n
T3106<\/td>\nEGFR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3107<\/td>\nERBB4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3108<\/td>\nEPHA2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3109<\/td>\nEPHB1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3110<\/td>\nEPHB2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3111<\/td>\nTYK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3112<\/td>\nEPHB4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3113<\/td>\nFER Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3115<\/td>\nLYN Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3116<\/td>\nZAP70 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3117<\/td>\nTIE1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3118<\/td>\nKDR Stable Ba\/F3 Cell Line (IL-3 Independent)<\/td>\n<\/tr>\n
T3119<\/td>\nTNK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3120<\/td>\nCSK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3121<\/td>\nMATK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3122<\/td>\nDDR1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3123<\/td>\nDDR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3124<\/td>\nPTK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3126<\/td>\nEPHA10 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3127<\/td>\nEPHA3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3128<\/td>\nEPHA6 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3129<\/td>\nEPHB3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3131<\/td>\nPTK2B Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3132<\/td>\nFGFR4 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3133<\/td>\nINSRR Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3134<\/td>\nAATK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3136<\/td>\nLMTK3 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3137<\/td>\nMuSK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3138<\/td>\nCSF1R Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3139<\/td>\nPTK7 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3140<\/td>\nROR1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3141<\/td>\nROR2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3142<\/td>\nRYK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3143<\/td>\nFRK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3145<\/td>\nSRMS Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3146<\/td>\nBMX Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3147<\/td>\nBTK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3148<\/td>\nITK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3149<\/td>\nTEC Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3150<\/td>\nTXK Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3151<\/td>\nTEK2 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3152<\/td>\nFLT1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3153<\/td>\nSTYK1 Stable Ba\/F3 Cell Line<\/td>\n<\/tr>\n
T3154<\/td>\nSPINK1 Stable AsPC-1 Cell Line<\/td>\n<\/tr>\n
T3155<\/td>\nhSSAO\/VAP-1 A7r5 Stable Cell Line<\/td>\n<\/tr>\n
T3156<\/td>\nMouse R-spondin1 Stable 293T Cell Line<\/td>\n<\/tr>\n
T3157<\/td>\nHuman T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line<\/td>\n<\/tr>\n
T3158<\/td>\nTrkA Stable T17 Cell Line<\/td>\n<\/tr>\n
T3159<\/td>\nTrkB Stable T48 Cell Line<\/td>\n<\/tr>\n
T3160<\/td>\nLuc-Akt-PH Stable MCF7 Cell Line<\/td>\n<\/tr>\n
T3161<\/td>\nLuc-Akt-PH\/YFP Stable MCF-7\/B2 Stable Cell Line<\/td>\n<\/tr>\n
T3162<\/td>\nHEK293S-TetR Stably Expressing TP Cell Line<\/td>\n<\/tr>\n
T3163<\/td>\nHEK293S-TetR GnTI- Stably Expressing TP Cell Line<\/td>\n<\/tr>\n
T3164<\/td>\nHEK293S-TetR Stably Expressing A160T Cell Line<\/td>\n<\/tr>\n
T3165<\/td>\nHEK293S-TetR GnTI- Stably Expressing A160T Cell Line<\/td>\n<\/tr>\n
T3166<\/td>\nCx26 Stable BxPC-3 Cell Line<\/td>\n<\/tr>\n
T3167<\/td>\nE-cadherin Stable MIA PaCa-2 Cell Line<\/td>\n<\/tr>\n
T3168<\/td>\nBMP9 Stable HEK293 Cell Line<\/td>\n<\/tr>\n
T3169<\/td>\n92 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line<\/td>\n<\/tr>\n
T3170<\/td>\n88 Tag Stable Y-LIZ DNA Polymerase-\u03b2 Knockout Mouse Embryonic Fibroblast Cell Line<\/td>\n<\/tr>\n
T3171<\/td>\nSIRT1 Wiltype Stable RKO Cell Line<\/td>\n<\/tr>\n
T3172<\/td>\nSIRT1 Knockout Stable RKO Cell Line<\/td>\n<\/tr>\n
T3173<\/td>\nSIRT1 Wildtype Stable DLD-1 Cell Line<\/td>\n<\/tr>\n
T3174<\/td>\nSIRT1 Knockout Stable DLD-1 Cell Line<\/td>\n<\/tr>\n
T3175<\/td>\nSIRT1 Wildtype Stable 293T Cell Line<\/td>\n<\/tr>\n
T3176<\/td>\nSIRT1 Knockout Stable 293T Cell Line<\/td>\n<\/tr>\n
T3177<\/td>\nPZ-TR Stable HepG2 Luciferase Reporter Cell Line<\/td>\n<\/tr>\n
T3178<\/td>\nAd5 pTP Stable 293 (293pTP) Cell Line<\/td>\n<\/tr>\n
T3181<\/td>\np47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line<\/td>\n<\/tr>\n
T3182<\/td>\nPodoplanin Stably Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3183<\/td>\nStable Retinal Pigment Epithelial Expressing Truncated hELG1-CFP Cell Line<\/td>\n<\/tr>\n
T3184<\/td>\nStable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line<\/td>\n<\/tr>\n
T3185<\/td>\nStable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line<\/td>\n<\/tr>\n
T3186<\/td>\nStable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line<\/td>\n<\/tr>\n
T3187<\/td>\nStable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line<\/td>\n<\/tr>\n
T3188<\/td>\nStable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line<\/td>\n<\/tr>\n
T3189<\/td>\nStable VERO Overexpressing HER2 Cell Line<\/td>\n<\/tr>\n
T3190<\/td>\nStable VERO Overexpressing HER3 Cell Line<\/td>\n<\/tr>\n
T3191<\/td>\nStable VERO Overexpressing HER2 and HER3 Cell Line<\/td>\n<\/tr>\n
T3192<\/td>\nStable VERO Overexpressing HER1 Cell Line<\/td>\n<\/tr>\n
T3193<\/td>\nStable CHO Overexpressing HER2 Cell Line<\/td>\n<\/tr>\n
T3194<\/td>\nStable VERO Overexpressing HER1 and HER2 Cell Line<\/td>\n<\/tr>\n
T3195<\/td>\nStable HepG2 Expressing Murine ADH1 (VA-13) Cell Line<\/td>\n<\/tr>\n
T3196<\/td>\nStable HA-Tagged hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3197<\/td>\nStable G90E Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3198<\/td>\nStable V94M Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3199<\/td>\nStable C307Y Mutated hGALE Expressing CHO Cell Line<\/td>\n<\/tr>\n
T3203<\/td>\nCRE Expressing 293 Cell Line<\/td>\n<\/tr>\n
T3207<\/td>\nICP47 Stable Raji Cell Line<\/td>\n<\/tr>\n
T3208<\/td>\nICP47 Stable Mel JuSo Cell Line<\/td>\n<\/tr>\n
T3209<\/td>\nHLA-DQ8 Expressing Stable K562 Cell Line<\/td>\n<\/tr>\n
T3210<\/td>\nMutant AKT1 Stable Proteus Syndrome Derived Cell Line (F6B)<\/td>\n<\/tr>\n
T3217<\/td>\nGFP Stably Expressing Schwann cells<\/td>\n<\/tr>\n
T3218<\/td>\nhuGRPr Expessing Stable Ace-1 Cell Line<\/td>\n<\/tr>\n
T3219<\/td>\nApolipoprotein A-1 Expressing Stable MC38 Cell Line (MC38 Apo)<\/td>\n<\/tr>\n
T3220<\/td>\nApolipoprotein A-1 and P144 Over-expressing Stable MC38 Cell Line (MC38 ApoLinkerP144)<\/td>\n<\/tr>\n
T3222<\/td>\nBRCA1 L1780P Mutant Stable HCC1937 Cell Line<\/td>\n<\/tr>\n
T3223<\/td>\nBRCA1 L1780P Mutant Stable HCC1395 TNBC Cell Line<\/td>\n<\/tr>\n
T3224<\/td>\nNDUFS2 Stable Knockout HEK293 Cell Line (HEK293\u0394NDUFS2)<\/td>\n<\/tr>\n
T3229<\/td>\nHEK293-T Cells Stably Expressing MLL-AF6 (MA6)<\/td>\n<\/tr>\n
T3230<\/td>\nHEK293-T Cells Stably Expressing AF6-MLL (A6M)<\/td>\n<\/tr>\n
T3231<\/td>\nHEK293-T Cells Stably Expressing MLL-AF6\/AF6-MLL (CO1)<\/td>\n<\/tr>\n
T3232<\/td>\nHEK293-T Cells Stably Expressing exMLL-AF6 (exMA6)<\/td>\n<\/tr>\n
T3233<\/td>\nHEK293-T Cells Stably Expressing AF6-shMLL (A6shM)<\/td>\n<\/tr>\n
T3234<\/td>\nHEK293-T Cells Stably Expressing exMLL-AF6\/AF6-shMLL (CO2)<\/td>\n<\/tr>\n
T3235<\/td>\nA549 Cells Stably Expressing hACE2 Receptor and TMPRSS2 (A549-AT)<\/td>\n<\/tr>\n
T3236<\/td>\nU2OS Stably Expressing NONO-APEX2 Cell Line (U2OS:NONO-APEX2-HA)<\/td>\n<\/tr>\n
T3241<\/td>\nts-46 Stable Knockout HEK293 Cell Line (ts-46 293KO)<\/td>\n<\/tr>\n
T3242<\/td>\nts-101 Stable Knockout HEK293 Cell Line (ts-101 293KO)<\/td>\n<\/tr>\n
T3243<\/td>\nHuman Progerin Expressing 3T3-L1 Stable Cell Line (3T3L1-PG)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Abmgood\u4e0d\u673d\u7cfb\u5217\u5546\u54c1 (immortalized cell)-\u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96\u7dad\u6bcd\u7d30\u80de \u6c38\u751f\u5316\u4eba\u985e\u5fc3\u81df\u7e96 […]<\/p>\n","protected":false},"author":3,"featured_media":28511,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373],"tags":[6527],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/48403"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=48403"}],"version-history":[{"count":7,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/48403\/revisions"}],"predecessor-version":[{"id":50771,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/48403\/revisions\/50771"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28511"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=48403"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=48403"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=48403"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}