{"id":47525,"date":"2023-11-30T17:58:34","date_gmt":"2023-11-30T09:58:34","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=47525"},"modified":"2025-09-16T14:55:12","modified_gmt":"2025-09-16T06:55:12","slug":"%e9%9b%99%e8%9e%a2%e5%85%89%e7%b4%a0%e9%85%b6%e5%a0%b1%e5%91%8a%e5%9f%ba%e5%9b%a0%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-dual-luciferase-reporter-gene-assay-kit%e8%b2%a8%e8%99%9fkta8010","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=47525","title":{"rendered":"\u96d9\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2 | Dual Luciferase Reporter Gene Assay Kit\u8ca8\u865fKTA8010"},"content":{"rendered":"

\u96d9\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2 |Dual Luciferase Reporter Gene Assay Kit\u8ca8\u865fKTA8010<\/span><\/strong><\/a><\/p>\n

Dual Luciferase Reporter Gene Assay Kit<\/span><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/span><\/strong>\u96d9\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2( Dual Luciferase Reporter Gene Assay Kit, KTA8010) \u5177\u6709\u9748\u654f\u3001\u5feb\u901f\u3001\u65b9\u4fbf\uff0c\u53ef\u6aa2\u6e2c\u96d9\u5831\u544a\u57fa\u56e0( Reporter gene )\u3002 \u96d9\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u7cfb\u7d71\u63d0\u4f9b\u4e86\u57f7\u884c\u5169\u7a2e\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u7684\u6709\u6548\u65b9\u6cd5\u3002\u5f9e\u55ae\u4e00\u6a23\u672c\u4f9d\u5e8f\u6e2c\u91cf\u87a2\u706b\u87f2 ( Firefly Luciferase ) \u548c\u6d77\u814e \u87a2\u5149\u7d20\u9176( Renilla Luciferase)\u7684\u6d3b\u6027\u3002\u900f\u904e\u6aa2\u6e2c\u87a2\u5149\u7d20\u9176(Luciferase)\u7684\u6d3b\u6027\u4f86\u5224\u65b7\u85e5\u7269\u6cbb\u7642\u5c0d\u76ee\u6a19\u57fa\u56e0\u7684\u8f49\u9304\u8abf\u63a7\u4f5c\u7528\u3002\u6d77\u814e\u87a2\u5149\u7d20\u9176(Renilla luciferase)\u6aa2\u6e2c\u8f49\u67d3\u6548\u7387\u7684\u5167\u53c3( internal reference )\uff0c\u4ee5\u6d88\u9664\u7d30\u80de\u6578\u91cf\u8207\u8f49\u67d3\u6548\u7387\u4e4b\u9593\u7684\u5dee\u7570\u3002<\/p>\n

\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176( Firefly luciferase)\u53ef\u4ee5\u50ac\u5316\u87a2\u706b\u87f2\u87a2\u5149\u7d20(firefly luciferin)\u751f\u6210\u6c27\u5316\u87a2\u5149\u7d20(Oxyluciferin) (\u5728ATP\u3001\u9382\u96e2\u5b50\u548c\u6c27\u6c23\u5b58\u5728\u7684\u60c5\u6cc1)\uff0c\u6c27\u5316\u87a2\u5149\u7d20( oxidation of luciferin)\u904e\u7a0b\u7522\u751f\u5149\u8a0a\u865f\u3002\u5728\u6709\u6c27(oxygen)\u5b58\u5728\u7684\u60c5\u6cc1\u4e0b\uff0c\u6d77\u814e\u87a2\u5149\u7d20\u9176(Renilla luciferase)\u53ef\u4ee5\u50ac\u5316coelenterazine\u6c27\u5316\u70bacoelenteramide\uff0c coelenterine\u6c27\u5316\u904e\u7a0b\u4e2d\u4e5f\u6703\u7522\u751f\u5149\u8a0a\u865f\u3002\u900f\u904e\u87a2\u5149\u7d20\u9176\u53ca\u5176\u5e95\u7269\u7684\u751f\u7269\u767c\u5149\u7cfb\u7d71\uff0c\u53ef\u4ee5\u975e\u5e38\u9748\u654f\u4e14\u6709\u6548\u7387\u3002\u901a\u5e38\uff0c\u5c07\u76ee\u7684\u57fa\u56e0\u7684\u8f49\u9304\u8abf\u63a7\u5143\u4ef6( Transcriptional regulation element)\u62165′-\u555f\u52d5\u5b50\u5340(5′-promoter region)\u514b\u9686\u5230\u87a2\u5149\u7d20\u9176(Luciferase )\u4e0a\u6e38\uff0c\u6216\u5c073′-UTR\u5340\u514b\u9686\u5230\u87a2\u5149\u7d20\u9176(Luciferase )\u4e0b\u6e38\uff0c\u5efa\u69cb\u5831\u544a\u57fa\u56e0\u8cea\u9ad4 ( Reporter gene plasmid)\uff0c\u7136\u5f8c\u9032\u884c\u8f49\u67d3(transfection)\u7d30\u80de\uff0c\u8655\u7406\u7528\u9069\u7576\u7684\u85e5\u7269\u7d30\u80de\uff0c\u7136\u5f8c\u88c2\u89e3\u7d30\u80de\u3002\u900f\u904e\u6aa2\u6e2c\u87a2\u5149\u7d20\u9176(Luciferase)\u7684\u6d3b\u6027\u4f86\u5224\u65b7\u85e5\u7269\u6cbb\u7642\u5c0d\u76ee\u6a19\u57fa\u56e0\u7684\u8f49\u9304\u8abf\u63a7\u4f5c\u7528\u3002\u6d77\u814e\u87a2\u5149\u7d20\u9176(Renilla luciferase)\u7528\u4f5c\u6aa2\u6e2c\u8f49\u67d3\u6548\u7387\u7684\u5167\u53c3( internal reference )\uff0c\u4ee5\u6d88\u9664\u7d30\u80de\u6578\u91cf\u8207\u8f49\u67d3\u6548\u7387\u4e4b\u9593\u7684\u5dee\u7570\u3002\u96d9\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2\u9996\u5148\u4ee5\u87a2\u5149\u7d20(Luciferin)\u70ba\u5e95\u7269\u6aa2\u6e2c\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly luciferase)\u7684\u6d3b\u6027\uff0c\u7136\u5f8c\u6dfb\u52a0\u6291\u5236\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Fireflyluciferase)\u50ac\u5316\u7684\u7269\u8cea\uff0c\u540c\u6642\u6dfb\u52a0 Coelenterazine \u6aa2\u6e2c\u6d77\u814e\u87a2\u5149\u7d20\u9176( Renilla luciferase)\u7684\u6d3b\u6027\uff0c\u5f9e\u800c\u5be6\u73fe\u96d9\u87a2\u5149\u7d20\u9176\u7684\u6aa2\u6e2c\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u3002\u8a72\u8a66\u5291\u76d2\u5177\u6709\u6aa2\u6e2c\u5feb\u901f\u3001\u9748\u654f\u5ea6\u9ad8\u3001\u6aa2\u6e2c\u7bc4\u570d\u5ee3\u3001\u4e0d\u53d7\u7d30\u80de\u5167\u6d3b\u6027\u5e72\u64fe\u7684\u7279\u6027\u3002<\/p>\n

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\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nDual Luciferase Reporter Gene Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nUsually, the Transcriptional regulation element or 5′-promoter region of the gene of interest is cloned upstream of Luciferase, or the 3′- UTR region is cloned downstream of Luciferase to construct a Reporter gene plasmid, and then transfect the cell, treat the cell with appropriate drugs, and then Lytic cells. The Transcriptional regulation effect of drug treatment on the target gene is judged by detecting the activity of Luciferase.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022Lysis Buffer (5\u00d7)\u2022Firefly Luciferase Assay Buffer\u2022Firefly Luciferase Substrate\u2022Renilla Luciferase Assay Buffer\u2022Renilla Luciferase Substrate (50\u00d7)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High luminous intensity.\u00a0\u2022 High detection sensitivity, wide linear range of the measured sample.\u00a0\u2022 Easy operation, stable reading, fast speed.\u00a0\u2022 Strong compatibility with various media.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStored at -20\u2103 for 12 months, protected from light<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nIn the presence of ATP, magnesium ions and oxygen, Firefly luciferase can catalyze firefly luciferin to Oxyluciferin, which generate light signals during the oxidation of luciferin. In the presence of oxygen, Renilla luciferase can catalyze the oxidation of coelenterazine to coelenteramide, and light signals are also generated during the oxidation of coelenterine. Through the Bioluminescence system of Luciferase and its substrate, gene expression can be detected very sensitively and efficiently.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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