{"id":47206,"date":"2023-11-20T16:02:37","date_gmt":"2023-11-20T08:02:37","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=47206"},"modified":"2023-11-27T16:54:15","modified_gmt":"2023-11-27T08:54:15","slug":"mrc2-orf-vector-mouse-porf-%e8%b2%a8%e8%99%9f30388014","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=47206","title":{"rendered":"Mrc2 ORF Vector (Mouse) (pORF) \u00a0| \u8ca8\u865f30388014\u00a0"},"content":{"rendered":"

Mrc2 ORF Vector (Mouse) (pORF) <\/strong>\u00a0| <\/strong>\u8ca8\u865f30388014<\/strong><\/a><\/span>\u00a0(1.0 \u00b5g DNA)<\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846(Open Reading Frame, ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2( Lentivirus<\/a>)\u548cAAV<\/a>\u3001\u817a\u75c5\u6bd2( Adenovirus<\/a>\u00a0)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

<\/p>\n

Mrc2 ORF Vector (Mouse) (pORF)\u8f09\u9ad4\u4ecb\u7d39: Accession Number NM_008626\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\n30388014<\/span><\/td>\n<\/tr>\n
Name<\/td>\nMrc2 ORF Vector (Mouse) (pORF)<\/td>\n<\/tr>\n
Unit<\/td>\n1.0 \u00b5g DNA<\/td>\n<\/tr>\n
Description<\/td>\nThe Mrc2 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/td>\n<\/tr>\n
Gene Name<\/td>\nMRC2<\/td>\n<\/tr>\n
Gene Full Name<\/td>\nmannose receptor, C type 2<\/td>\n<\/tr>\n
Alias<\/td>\nKIAA0709, ENDO180, CLEC13E, CD280<\/td>\n<\/tr>\n
Accession Number<\/td>\nNM_008626<\/td>\n<\/tr>\n
Species<\/td>\nMouse<\/td>\n<\/tr>\n
Vector Map<\/td>\npORF\u00a0<\/a>(click blue link to view)<\/td>\n<\/tr>\n
System<\/td>\nNon-Viral Vector<\/td>\n<\/tr>\n
Insert Size<\/td>\n4440<\/td>\n<\/tr>\n
Vector Size<\/td>\n2596<\/td>\n<\/tr>\n
Storage Condition<\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

<\/p>\n\n\n\n\n\n\n
Vector Name<\/b><\/td>\npORF<\/td>\n<\/tr>\n
Antibiotic Information<\/b><\/td>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
Sequencing Primers<\/b><\/td>\nM13 FP: 5′-CCCAGTCACGACGTTGTAAAACG-3′
\nM13 RP: 5′-CAGGAAACAGCTATGAC-3′<\/td>\n<\/tr>\n
Additional Information<\/b><\/td>\nNo Additional Information<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Lenti- backbones<\/td>\nBlank Control Lentivector<\/td>\nCat. No<\/td>\nBlank Control Virus<\/td>\nCat. No<\/td>\n<\/tr>\n
pLenti-GIII-CMV<\/a><\/td>\npLenti-III-Blank<\/a><\/td>\nLV587<\/td>\nLenti-III-Blank Lentivirus<\/a><\/td>\nLVP687<\/td>\n<\/tr>\n
pLenti-GIII-CMV-C-term-HA<\/a><\/td>\npLenti-III-Blank<\/a><\/td>\nLV587<\/td>\nLenti-III-Blank Lentivirus<\/a><\/td>\nLVP687<\/td>\n<\/tr>\n
pLenti-GIII-CMV-GFP-2A-Puro<\/a><\/td>\npLenti-CMV-GFP-2A-Puro-Blank Vector<\/a><\/td>\nLV590<\/td>\nLenti-CMV-GFP-2A-Puro-Blank Lentivirus<\/a><\/td>\nLVP690<\/td>\n<\/tr>\n
pLenti-GIII-CMV-RFP-2A-Puro<\/a><\/td>\npLenti-CMV-RFP-2A-Puro-Blank Vector<\/a><\/td>\nLV591<\/td>\nLenti-CMV-RFP-2A-Puro-Blank Lentivirus<\/a><\/td>\nLVP691<\/td>\n<\/tr>\n
pLenti-GIII-EF1a<\/a><\/td>\npLenti-EF1a-Blank Vector<\/a><\/td>\nLV588<\/td>\nLenti-EF1a-Blank Lentivirus<\/a><\/td>\nLVP688<\/td>\n<\/tr>\n
pLenti-GIII-UbC<\/a><\/td>\npLenti-UbC-Blank Vector<\/a><\/td>\nLV589<\/td>\nLenti-UbC-Blank Lentivirus<\/a><\/td>\nLVP689<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
Other Reporter Control Lentivectors<\/td>\nCat. No<\/td>\n<\/tr>\n
Blank Promoter-Reporter Lentiviral Vector (GFP)<\/a><\/td>\nLV654<\/td>\n<\/tr>\n
\u00a0 Blank Promoter-Reporter Lentiviral Vector (Luc)<\/a><\/td>\nLV653<\/td>\n<\/tr>\n
\u00a0 CMV Promoter-Reporter Lentiviral Vector (GFP)<\/a><\/td>\nLV656<\/td>\n<\/tr>\n
\u00a0 CMV Promoter-Reporter Lentiviral Vector (Luc)<\/a><\/td>\nLV655<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
AAV- Backbones<\/td>\nBlank Control AAV<\/td>\nCat. No<\/td>\nSearch Viruses by Serotype<\/td>\n<\/tr>\n
pAAV-G-CMV-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (Amp)<\/a><\/td>\nAAV001a<\/td>\nAAV Blank Control Virus (CMV) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (GFP) (Amp)<\/a><\/td>\nAAV003a<\/td>\nAAV Blank Control Virus (CMV) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (Luc) (Amp)<\/a><\/td>\nAAV002a<\/td>\nAAV Blank Control Virus (CMV) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV016a<\/td>\nAAV Blank Control Virus (CMV) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (Amp)<\/a><\/td>\nAAV007a<\/td>\nAAV Blank Control Virus (EF1a) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (GFP) (Amp)<\/a><\/td>\nAAV009a<\/td>\nAAV Blank Control Virus (EF1a) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (Luc) (Amp)<\/a><\/td>\nAAV008a<\/td>\nAAV Blank Control Virus (EF1a) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV018a<\/td>\nAAV Blank Control Virus (EF1a) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (Amp)<\/a><\/td>\nAAV022a<\/td>\nAAV Blank Control Virus (hSync) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (SV40-GFP) (Amp)<\/a><\/td>\nAAV024a<\/td>\nAAV Blank Control Virus (hSync) (SV40-GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (SV40-Luc) (Amp)<\/a><\/td>\nAAV023a<\/td>\nAAV Blank Control Virus (hSync) (SV40-Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV025a<\/td>\nAAV Blank Control Virus (hSync) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (Amp)<\/a><\/td>\nAAV004a<\/td>\nAAV Blank Control Virus (PGK) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (GFP) (Amp)<\/a><\/td>\nAAV006a<\/td>\nAAV Blank Control Virus (PGK) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (Luc) (Amp)<\/a><\/td>\nAAV005a<\/td>\nAAV Blank Control Virus (PGK) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV020a<\/td>\nAAV Blank Control Virus (PGK) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CAGGS) (Amp)<\/a><\/td>\nAAV013a<\/td>\nAAV Blank Control Virus (CAGGS) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CAGGS) (GFP) (Amp)<\/a><\/td>\nAAV014a<\/td>\nAAV Blank Control Virus (CAGGS) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-CBH-gcGFP Amp<\/a><\/td>\n\u00a0 AAV Blank Control Vector (CAGGS) (SV40-GFP) (Amp)<\/a><\/td>\nAAV021a<\/td>\nAAV Blank Control Virus (CAGGS) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Mrc2 ORF Vector (Mouse) (pORF) \u00a0| \u8ca8\u865f30388014\u00a0(1.0 \u00b5g DN […]<\/p>\n","protected":false},"author":3,"featured_media":29552,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373],"tags":[6375],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47206"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=47206"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47206\/revisions"}],"predecessor-version":[{"id":49652,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/47206\/revisions\/49652"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29552"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=47206"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=47206"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=47206"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}