{"id":46187,"date":"2023-10-18T12:26:41","date_gmt":"2023-10-18T04:26:41","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=46187"},"modified":"2023-10-27T17:40:30","modified_gmt":"2023-10-27T09:40:30","slug":"%e7%a2%98%e5%8c%96%e4%b8%99%e5%95%b6propidium-iodide-pi-servicebio-%e8%b2%a8%e8%99%9fg1021-10ml","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=46187","title":{"rendered":"\u7898\u5316\u4e19\u5576(propidium iodide, PI) | SERVICEBIO \u8ca8\u865fG1021-10ML"},"content":{"rendered":"

SERVICEBIO\u7d30\u80de\u87a2\u5149\u67d3\u8272(Cell Fluorescence Staining)\u7cfb\u5217\u7522\u54c1-\u7898\u5316\u4e19\u5576(propidium iodide, PI)<\/span><\/span><\/span><\/strong><\/span><\/p>\n

\u7898\u5316\u4e19\u5576(propidium iodide, PI) |SERVICEBIO \u8ca8\u865fG1021-10ML<\/a><\/span><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong><\/span><\/p>\n

propidium iodide, PI\uff08\u6216\u7a31\u7898\u5316\u4e19\u5576)\u662f\u6eb4\u5316\u4e59\u9320( ethidium bromide)\u7684\u985e\u4f3c\u7269\uff0c\u53ef\u8207 DNA \u7262\u56fa\u7d50\u5408\u3002\u7576\u5b83\u5d4c\u5165\u96d9\u80a1DNA( double-stranded DNA)\u6642\uff0c\u5b83\u6703\u91cb\u653e\u7d05\u8272\u87a2\u5149\u4f86\u67d3\u8272DNA\u6216\u7d30\u80de\u6838\u3002 \u7898\u5316\u4e19\u5576(propidium iodide, PI)\u7121\u6cd5\u7a7f\u900f\u5b8c\u6574\u7684\u7d30\u80de\u819c(intact cell membrane)\uff0c\u4f46\u53ef\u4ee5\u7a7f\u900f\u51cb\u4ea1\u665a\u671f\u7d30\u80de( late apoptotic cells )\u548c\u6b7b\u4ea1\u7d30\u80de(dead cells)\u7684\u53d7\u640d\u7d30\u80de\u819c\u3002\u9451\u65bc\u6b64\u7279\u9ede\uff0c\u7898\u5316\u4e19\u5576(propidium iodide, PI)\u901a\u5e38\u8207Calcein-AM\u6216FDA\u7b49\u87a2\u5149\u63a2\u91dd\u7d50\u5408\u4f7f\u7528\uff0c\u5c0d\u6b7b\u7d30\u80de\u9032\u884c\u67d3\u8272\u89c0\u5bdf\uff0c\u6216\u63a1\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u5c0d\u7d30\u80de\u51cb\u4ea1( apoptosis)\u548c\u7d30\u80de\u9031\u671f(cell cycle)\u9032\u884c\u76f8\u5c0d\u5b9a\u91cf\u6aa2\u6e2c\u3002 PI-\u96d9\u80a1DNA\u8907\u5408\u7269\u7684\u6700\u5927\u6fc0\u767c\u6ce2\u9577\u70ba535nm\uff0c\u6700\u5927\u767c\u5c04\u6ce2\u9577\u70ba615nm\u3002\u672c\u7522\u54c1\u7898\u5316\u4e19\u5576(propidium iodide, PI)\u67d3\u8272\u6db2\u70ba\u5373\u7528\u578b\uff0c\u6fc3\u5ea6\u70ba100 \u03bcg\/mL\uff0c\u53ef\u76f4\u63a5\u7528\u65bc\u58de\u6b7b\u7d30\u80de\u6216\u7d44\u7e54\u7684\u7d30\u80de\u6838\u67d3\u8272\uff0c\u7d30\u80de\u61f8\u6d6e\u6db2\u53ef\u7528\u65bc\u6d41\u5f0f( flow cytometry)\u6aa2\u6e2c\u7d30\u80de\u9031\u671f\u7528\u6b64\u67d3\u8272\u6db2\u67d3\u8272\u5f8c\u9032\u884c\u7d30\u80de\u8a08\u6578\u3002<\/p>\n

<\/p>\n\n\n\n\n
Product Name<\/strong><\/td>\nCat.No<\/strong><\/td>\nSpec.<\/strong><\/td>\n<\/tr>\n
PI Staining Solution<\/td>\nG1021-10ML<\/td>\n10 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Assay Protocol<\/strong><\/span><\/p>\n

lFlow Cytometry Assays For Cell Cycle Procedures\uff1a<\/p>\n

1. The cultured viable cells were digested, washed with PBS, and collected by low-speed centrifugation to obtain cell precipitation.<\/p>\n

2. Slowly add 1-3 mL of 90% ethanol precooled at 20 \u00b0 C to the cell precipitation, resuspended the cells, and ice bath overnight.<\/p>\n

3. Cells were collected by centrifugation at 1500 for 5min, resuspended in PBS, and centrifuged again to remove the supernatant.<\/p>\n

4. Cells were resuspended with 250 \u03bcL PBS.<\/p>\n

5. Add 2 \u03bcL of RNase A (G3405 and G3413 are recommended) at a concentration of 1 mg\/mL, and then water bath at 37\u2103 for 40 min.<\/p>\n

6. Add 50 \u00b5L PI dye solution and incubate in the dark for 20 min (the time can be adjusted according to the staining results of experimental materials).<\/p>\n

7. Flow cytometry detection.<\/p>\n

Fluorescence Microscopy to Observe The Procedure of Living Dead Cells\uff1a<\/strong><\/span><\/p>\n

1. After cultured adherent cells, the culture medium was removed and washed twice with PBS.<\/p>\n

2. The PI dye solution was diluted 10-20 times with PBS to prepare a final concentration of 5-10 \u03bcg\/mL PI staining working solution. An appropriate amount of PI staining working solution was added to the well plate to cover the cells, and the cells were incubated for 5-10 min at room temperature in the dark.<\/p>\n

3. The staining work was abandoned, and the cells were covered with PBS and observed by fluorescence microscope. Nuclei of dead or late apoptotic cells show red fluorescence.<\/p>\n

\u6d41\u5f0f\u7d30\u80de\u5100\u5075\u6e2c\u7d30\u80de\u9031\u671f\u7a0b\u5e8f\uff1a<\/span><\/strong><\/span><\/p>\n

1.\u5c07\u57f9\u990a\u7684\u6d3b\u7d30\u80de\u6d88\u5316\uff0cPBS\u6d17\u6ecc\uff0c\u4ee5\u4f4e\u901f\u96e2\u5fc3\u6536\u96c6\uff0c\u5f97\u5230\u7d30\u80de\u6c89\u6fb1\u3002<\/p>\n

2. \u5c07\u7d30\u80de\u6c89\u6fb1\u7de9\u6162\u52a0\u5165\u9810\u51b7\u65bc20\u2103\u76841-3mL 90%\u4e59\u9187\uff0c\u91cd\u61f8\u7d30\u80de\uff0c\u51b0\u6d74\u904e\u591c\u3002<\/p>\n

3. 1500\u2103\u96e2\u5fc35min\u6536\u96c6\u7d30\u80de\uff0c\u91cd\u61f8\u65bcPBS\u4e2d\uff0c\u518d\u6b21\u96e2\u5fc3\u53bb\u9664\u4e0a\u6e05\u6db2\u3002<\/p>\n

4.\u7528250\u03bcL PBS\u91cd\u61f8\u7d30\u80de\u3002<\/p>\n

5. \u52a0\u5165 2 \u03bcL RNase A\uff08\u5efa\u8b70 G3405 \u548c G3413\uff09\uff0c\u6fc3\u5ea6\u70ba 1 mg\/mL\uff0c\u7136\u5f8c 37\u2103\u6c34\u6d74 40 \u5206\u9418\u3002<\/p>\n

6. \u52a0\u516550 \u00b5L PI\u67d3\u6599\u6eb6\u6db2\uff0c\u907f\u5149\u5b75\u80b220 min\uff08\u6642\u9593\u53ef\u4f9d\u5be6\u9a57\u6750\u6599\u67d3\u8272\u7d50\u679c\u8abf\u6574\uff09\u3002<\/p>\n

7.\u6d41\u5f0f\u7d30\u80de\u5100\u6aa2\u6e2c\u3002<\/p>\n

\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf\u6d3b\u6b7b\u7d30\u80de\u7684\u904e\u7a0b\uff1a<\/strong><\/span><\/p>\n

1.\u8cbc\u58c1\u7d30\u80de\u57f9\u990a\u5f8c\uff0c\u9664\u53bb\u57f9\u990a\u57fa\uff0c\u4ee5PBS\u6d17\u6ecc\u5169\u6b21\u3002<\/p>\n

2.\u5c07PI\u67d3\u6599\u6eb6\u6db2\u4ee5PBS\u7a00\u91cb10-20\u500d\uff0c\u88fd\u5099\u7d42\u6fc3\u5ea65-10 \u03bcg\/mL\u7684PI\u67d3\u8272\u5de5\u4f5c\u6db2\u3002\u5728\u5b54\u677f\u4e2d\u52a0\u5165\u9069\u91cfPI\u67d3\u8272\u5de5\u4f5c\u6db2\u8986\u84cb\u7d30\u80de\uff0c\u5ba4\u6eab\u907f\u5149\u5b75\u80b2\u7d30\u80de5-10 min\u3002<\/p>\n

3.\u653e\u68c4\u67d3\u8272\u5de5\u4f5c\uff0c\u7528PBS\u8986\u84cb\u7d30\u80de\u4e26\u5728\u87a2\u5149\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u3002\u6b7b\u4ea1\u6216\u665a\u671f\u51cb\u4ea1\u7d30\u80de\u7684\u7d30\u80de\u6838\u986f\u793a\u7d05\u8272\u87a2\u5149\u3002<\/p>\n

<\/p>\n

<\/div>\n
\n
\n

 <\/p>\n

<\/div>\n
\n
\n\n\n\n\n\n\n<\/colgroup>\n\n\n
\n
Cat.No.<\/div>\n<\/th>\n
\n
Product Name<\/div>\n<\/th>\n
\n
Spec.<\/div>\n<\/th>\n<\/tr>\n<\/thead>\n<\/table>\n<\/div>\n
\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n
G1011-10ML<\/span><\/div>\n<\/td>\n
\n
Hoechst 33258 Staining Solution (Ready-To-Use)
\nHoechst 33258 \u67d3\u8272\u6db2\uff08\u5373\u7528\u578b\uff09<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
10 mL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1012-100ML<\/span><\/div>\n<\/td>\n
\n
DAPI Staining Reagent (Ready to Use)
\nDAPI \u67d3\u8272\u8a66\u5291\uff08\u5373\u4f7f\u7528\u578b\uff09<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
100 mL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1012-10ML<\/span><\/div>\n<\/td>\n
\n
DAPI Staining Reagent (Ready to Use)
\nDAPI \u67d3\u8272\u8a66\u5291\uff08\u5373\u4f7f\u7528\u578b\uff09<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
10 mL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1021-10ML<\/span><\/div>\n<\/td>\n
\n
PI Stain
\n\u7898\u5316\u4e19\u5576(propidium iodide, PI) <\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
10 mL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1127-1ML<\/span><\/div>\n<\/td>\n
\n
Hoechst 33342 Staining Solution
\nHoechst 33342 \u67d3\u8272\u6db2<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
1 mL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1515-100T<\/span><\/div>\n<\/td>\n
\n
JC-1 Mitochondrial Membrane Potential Assay Kit
\nJC-1 \u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
100 T<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1704<\/span><\/div>\n<\/td>\n
\n
DiO Cell Membrane Green Fluorescence Staining Kit
\nDiO \u7d30\u80de\u819c\u7da0\u8272\u87a2\u5149\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
100-1000 T<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1705<\/span><\/div>\n<\/td>\n
\n
Dil Cell Membrane Red Fluorescence Staining Kit
\nDil \u7d30\u80de\u819c\u7d05\u8272\u87a2\u5149\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
100-1000 T<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1720-20UL<\/span><\/div>\n<\/td>\n
\n
ER-Tracker Green
\nER-\u8ffd\u8e64\u5668\u7da0\u8272(ER-Tracker Green)<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
20 \u03bcL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1721-20UL<\/span><\/div>\n<\/td>\n
\n
ER-Tracker Red
\nER-\u8ffd\u8e64\u5668\u7d05\u8272(ER-Tracker Red) <\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
20 \u03bcL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1722-50UL<\/span><\/div>\n<\/td>\n
\n
Lyso-Tracker Green
\n\u6eb6\u8840\u8ffd\u8e64\u5668\u7da0\u8272(Lyso-Tracker Green) <\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
50 \u03bcL<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1723-50UG<\/span><\/div>\n<\/td>\n
\n
MitoTracker Red CMXRos
\nMitoTracker \u7d05\u8272<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
50 \u03bcg<\/span><\/div>\n<\/td>\n<\/tr>\n
\n
G1905-100T<\/span><\/div>\n<\/td>\n
\n
Lipid Droplets Fluorescence Assay Kit
\n\u8102\u6ef4\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span>
\n<\/span><\/div>\n<\/td>\n
\n
100 T<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

<\/p>\n

Servicebio\u53f0\u7063\u54c1\u724c\u4ecb\u7d39-BRAND\/AGENCY\/\u54c1\u724c\u4ecb\u7d39- Servicebio<\/span><\/span><\/strong><\/p>\n\n\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Classfication<\/td>\nCat No.<\/td>\nName<\/td>\nPlace of Origin<\/td>\nSpec.<\/td>\n<\/tr>\n
\n
Amino Acids<\/div>\n<\/td>\n
GC304002<\/td>\nL-Glutamine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC304003<\/td>\nL-Arginine hydrochloride<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304004-100g<\/td>\nL-Glutamine acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC304005<\/td>\nL-Isoleucine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304006-25g<\/td>\nL-Leucine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304007<\/td>\nL-Lysine hydrochloride<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304009<\/td>\nL-Methionine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304010<\/td>\nL-Phenylalanine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC304011-25g<\/td>\nL-Alanine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304012<\/td>\nL-Histidine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304013<\/td>\nL-Serine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304014<\/td>\nL-tryptophan<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC304016<\/td>\nL-Threonine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304017<\/td>\nL-Valine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC304019<\/td>\nGlycine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC304021<\/td>\nL-Histidine hydrochloride monohydrate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC304022<\/td>\nGlycine cell culture grade
\n\u7518\u6c28\u9178Glycine (\u7d30\u80de\u57f9\u990a\u7b49\u7d1a<\/td>\n		\u7522\u54c1\u8cc7\u6599<\/span><\/a><\/td>\n100 g<\/td>\n<\/tr>\n
\n
Protein Derivatives<\/div>\n<\/td>\n
GC305002-5g<\/td>\nIodoacetamide<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC305003<\/td>\nTrypsin 1:250 Source: Porcine Pancreas<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC305004-5g<\/td>\nPepsin 1:3000<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC305005<\/td>\nHorseradish peroxidase<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC305006<\/td>\nBovine Serum Albumin – import<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC305009<\/td>\nHuman Albumin-FITC<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n0.5 mL<\/td>\n<\/tr>\n
GC305010<\/td>\nBovine Serum Albumin BSA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC305010-25G<\/td>\nBovine Serum Albumin BSA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC305010-50G<\/td>\nBovine Serum Albumin BSA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n50 g<\/td>\n<\/tr>\n
GC305010-5G<\/td>\nBovine Serum Albumin BSA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC305013-100mg<\/td>\nCollagenase I<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mg<\/td>\n<\/tr>\n
GC305014-100mg<\/td>\nCollagenase II<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mg<\/td>\n<\/tr>\n
GC305015-100mg<\/td>\nCollagenase IV
\n\u81a0\u539f\u9176IV (Collagenase IV)<\/td>\n		\u7522\u54c1\u8cc7\u6599<\/a><\/span><\/td>\n100 mg<\/td>\n<\/tr>\n
GC305016-100g<\/td>\nGelatine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
\n
Hormones<\/div>\n<\/td>\n
GC302002-25mg<\/td>\nAbscisic acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 mg<\/td>\n<\/tr>\n
GC302003<\/td>\nAngiotensin II<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mg<\/td>\n<\/tr>\n
\n
Biological pigments<\/div>\n<\/td>\n
GC307002-25g<\/td>\nCrystal violet<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC307003-5g<\/td>\nTrypan Blue<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307005<\/td>\nAlcian Blue8GX<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307006-5g<\/td>\nBromphenol Blue<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307007<\/td>\nServa blue G-250<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307008-5g<\/td>\nCongo Red<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307009-25g<\/td>\nAlizarin Red S<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC307010-1g<\/td>\nGiemsa\u2032s Stain<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307011<\/td>\nFuchsin basic<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307012-10g<\/td>\nPonceau S<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307013<\/td>\nAcid red 26 2R<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307014<\/td>\nDirect Red 80<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307015-5g<\/td>\nSudan Black B<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307017<\/td>\nAniline blue<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307018<\/td>\nNuclear fast red<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307019<\/td>\nSolvent Blue 38<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307020<\/td>\nHematoxylin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307021<\/td>\nSolvent Red 43<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307022<\/td>\nEosin Y, water soluble<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307023<\/td>\nSafranin O<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307024<\/td>\nFast Green<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307026-5g<\/td>\nPyronin G<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307027-1g<\/td>\nJanus Green B<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307030-10g<\/td>\nBrilliant Cresyl Blue<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307031<\/td>\nCoomassie brilliant blue R-250<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307034-1g<\/td>\nThionine acetate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307035-1g<\/td>\nCresyl Fast Violet<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307036-1g<\/td>\nOrcein<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307037-1g<\/td>\nCarmine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC307039-10g<\/td>\nMordant Blue 29<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC307042-5g<\/td>\nOil Red O<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC307045-25g<\/td>\nRhodamine B<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
\n
Vitamins<\/div>\n<\/td>\n
GC301015-25g<\/td>\nVitamin C<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC303001<\/td>\nFolic acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC303004<\/td>\nRibofavin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC303005-5g<\/td>\nVitaminB6<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC303006-25g<\/td>\nVitaminB3<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC303008<\/td>\nVitaminB12<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC303011-25mg<\/td>\nNADPH-Na4<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 mg<\/td>\n<\/tr>\n
GC303013-250mg<\/td>\n\u03b2-nicotinamide adenine dinucleotide NAD<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n250 mg<\/td>\n<\/tr>\n
GC303014-250mg<\/td>\nNADH-Na2<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n250 mg<\/td>\n<\/tr>\n
\n
Antibiotics<\/div>\n<\/td>\n
GC301002<\/td>\nStreptozocin STZ<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301002-100mg<\/td>\nStreptozocin STZ<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mg<\/td>\n<\/tr>\n
GC301003<\/td>\nPenicillin G Sodium salt<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301004<\/td>\nAmpicillin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301005<\/td>\nStreptomycin sulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC301008<\/td>\nKanamycin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301009<\/td>\nKanamycin sulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC301009-1g<\/td>\nHygromycin B<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301011<\/td>\nGeneticin G-418<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC301013<\/td>\nChloramphenicol<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC301013-10mg<\/td>\nPseudomonic acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mg<\/td>\n<\/tr>\n
GC301014-5g<\/td>\nNorfloxacin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC301016<\/td>\nCisplatin<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mg<\/td>\n<\/tr>\n
GC301017-100mg<\/td>\nSirolimus<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mg<\/td>\n<\/tr>\n
\n
Heterocyclic compounds<\/div>\n<\/td>\n
GC207001<\/td>\nImidazole<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC207002<\/td>\nPMSF<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
GC207003<\/td>\nFreund’s Adjuvant Complete<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mL<\/td>\n<\/tr>\n
GC207004<\/td>\nFreund’s Adjuvant Incomplete<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mL<\/td>\n<\/tr>\n
\n
Salt<\/div>\n<\/td>\n
GC201001<\/td>\nGuanidine HCl<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC201002<\/td>\nGuanidine thiocyanate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
\n
Sugar Alcohols<\/div>\n<\/td>\n
GC205001<\/td>\nL(+)-Lactic acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC205002<\/td>\nPyruvic acid sodium salt<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC205003<\/td>\nD-Glucose, anhydrous<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC205004-10g<\/td>\nD(+)-Trehalose dihydrate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC205005<\/td>\nD(+)- Galactose<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC205006-10g<\/td>\nD-(-)Arabinose<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 g<\/td>\n<\/tr>\n
GC205007-1g<\/td>\nDTE<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC205008<\/td>\nGlycerol<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC205009<\/td>\nLPS<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mg<\/td>\n<\/tr>\n
GC205010<\/td>\nDTT<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC205011<\/td>\nIPTG<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
GC205013<\/td>\nAgarose<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC205014<\/td>\nSucrose<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC205015<\/td>\nD-Sorbitol<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
\n
Solvent<\/div>\n<\/td>\n
GC203001<\/td>\nTEMED<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC203002<\/td>\nDMSO(Dimethyl sulfoxide)<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC203003<\/td>\nUrea<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC203005<\/td>\nDMSO cellular level<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n10 mL<\/td>\n<\/tr>\n
\n
Complex<\/div>\n<\/td>\n
GC202001<\/td>\nEDTA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC202002<\/td>\nEDTA -Na2<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
\n
Buffer salt<\/div>\n<\/td>\n
GC208001<\/td>\nTris (Hydroxymethyl) Aminomethane<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC208003<\/td>\nRIS-HCL<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC208004-100g<\/td>\nBIS-TRIS<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC208006<\/td>\nN-Tris[Hydroxymethyl]Methylglycine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC208007<\/td>\n4-propanesulfonyl morpholine<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC208008-25g<\/td>\nHEPPS<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC208009<\/td>\nHEPES, Free acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC208012-1g<\/td>\nTCEP\u00b7HCL<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n1 g<\/td>\n<\/tr>\n
\n
Surfactant<\/div>\n<\/td>\n
GC204001<\/td>\nTween-80<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC204002<\/td>\nTween-20<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC204003<\/td>\nTriton X-100<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
GC204004<\/td>\nSodium dodecylsulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC204005<\/td>\nSodium dodecylsulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC204006<\/td>\nNonidet TM P40 Substitute NP-40<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC204007<\/td>\nDeoxycholic acid\uff0c sodium salt<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC204009-100ml<\/td>\nAPES<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 mL<\/td>\n<\/tr>\n
\n
Amine organics<\/div>\n<\/td>\n
GC206002<\/td>\nAcrylamide<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC206003<\/td>\nN’-MBA<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
\n
Polymer<\/div>\n<\/td>\n
GC209002-250g<\/td>\nPEG 8000<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n250 g<\/td>\n<\/tr>\n
GC209003-250g<\/td>\nPEG 6000<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n250 g<\/td>\n<\/tr>\n
GC209004-100g<\/td>\nPEG 3350<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC209005-500g<\/td>\nPoly(ethylene glycol)2000<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC209006-250g<\/td>\nPoly(ethylene glycol) 20000<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n250 g<\/td>\n<\/tr>\n
\n
Inorganic salt<\/div>\n<\/td>\n
GC106001<\/td>\nOrthoboric acid<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC104004<\/td>\nSodium bicarbonate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC108003<\/td>\nCitric acid monohydrate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC108004<\/td>\nSodium citrate dihydrate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC102001<\/td>\nCalcium chloride<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC102002<\/td>\nMagnesium chloride hexahydrate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC102003<\/td>\nPotassium chloride<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC102004<\/td>\nSodium chloride<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC101004<\/td>\nMagnesium Sulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
GC101013<\/td>\nAmmonium persulfate<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n25 g<\/td>\n<\/tr>\n
GC105001<\/td>\nSodium phosphate monobasic<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC105002<\/td>\nSodium Phosphate, Dibasic<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC105003<\/td>\nPotassium Phosphate Monobasic<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n500 g<\/td>\n<\/tr>\n
GC107001<\/td>\nSodium hydroxide<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n
<\/td>\nGC306001<\/td>\nLodixanol<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n5 g<\/td>\n<\/tr>\n
<\/td>\nGC310001-100g<\/td>\nskimmed milk powder<\/td>\n\u7522\u54c1\u8cc7\u6599<\/td>\n100 g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

SERVICEBIO\u7d30\u80de\u87a2\u5149\u67d3\u8272(Cell Fluorescence Staining)\u7cfb\u5217\u7522\u54c1-\u7898\u5316\u4e19\u5576(p […]<\/p>\n","protected":false},"author":3,"featured_media":46192,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5508],"tags":[6096,5203],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/46187"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=46187"}],"version-history":[{"count":12,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/46187\/revisions"}],"predecessor-version":[{"id":46565,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/46187\/revisions\/46565"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/46192"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=46187"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=46187"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=46187"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}