{"id":46076,"date":"2023-10-13T17:34:25","date_gmt":"2023-10-13T09:34:25","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=46076"},"modified":"2023-10-22T12:01:18","modified_gmt":"2023-10-22T04:01:18","slug":"sirt1-%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-sirt1-activity-assay-kit-abcam-%e8%b2%a8%e8%99%9fab156065","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=46076","title":{"rendered":"SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | SIRT1 Activity Assay Kit Abcam \u8ca8\u865f(ab156065)"},"content":{"rendered":"

SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | Abcam \u8ca8\u865f<\/strong><\/span><\/a>(ab156065)<\/a><\/strong><\/span><\/p>\n

SIRT1 Activity Assay Kit (Fluorometric) (ab156065)<\/p>\n

\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u5316\u9176 (Histone Deacetylases, HDAC) \u662f\u4e00\u985e\u8ca0\u8cac\u6838\u5fc3\u7d44\u86cb\u767d\uff08H2A\u3001H2B\u3001H3 \u548c H4\uff09N \u7aef\u90e8\u5206\u8cf4\u6c28\u9178\u6b98\u57fa\u53bb\u4e59\u91af\u5316\u7684\u9176\uff0c\u4f7f\u7d44\u86cb\u767d\u80fd\u5920\u66f4\u7dca\u5bc6\u5730\u5305\u88f9 DNA\u3002\u6839\u64da\u529f\u80fd\u548c DNA \u5e8f\u5217\u76f8\u4f3c\u6027\uff0cHDAC \u86cb\u767d\u5206\u70ba\u56db\u985e (class I, class IIA, class IIB, class III, class IV)\u3002I\u3001IIA \u548c IIB \u985e\u88ab\u8a8d\u70ba\u662f\u300c\u7d93\u5178\u300dHDAC\uff0c\u5176\u6d3b\u6027\u53d7\u5230\u66f2\u53e4\u6291\u83cc\u7d20 A (trichostatin A, TSA) \u7684\u6291\u5236\uff0c\u800c III \u985e\u662f\u4e0d\u53d7 TSA \u5f71\u97ff\u7684 NAD+ \u4f9d\u8cf4\u6027\u86cb\u767d (sirtuins) \u5bb6\u65cf\u3002\u50c5\u57fa\u65bc\u8207\u5176\u4ed6\u985e\u5225\u7684 DNA \u5e8f\u5217\u76f8\u4f3c\u6027\uff0cIV \u985e\u672c\u8eab\u5c31\u88ab\u8a8d\u70ba\u662f\u975e\u5178\u578b\u985e\u5225\u3002SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09(ab156065) \u53ef\u6aa2\u6e2c\u88c2\u89e3\u7269\u4e2d\u7684 SIRT1 \u6d3b\u6027(SIRT1 activity)\u3002<\/p>\n

SIRT1 \u662f\u4e00\u7a2e\u4fdd\u5b88\u86cb\u767d\uff0c\u6700\u8fd1\u88ab\u8b49\u660e\u53ef\u4ee5\u8abf\u7bc0\u82bd\u6b96\u9175\u6bcd( budding yeast)\u548c\u7dda\u87f2(nematode)\u7684\u58fd\u547d\u5ef6\u9577\u3002 2000\u5e74\uff0c\u6709\u5831\u544a\u6307\u51fa\u9175\u6bcdSIRT1\u86cb\u767d\u662f\u4e00\u7a2eNAD(+)\u4f9d\u8cf4\u6027\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u5316\u9176\uff0c\u5728\u8f49\u9304\u6c89\u9ed8\u3001\u57fa\u56e0\u7d44\u7a69\u5b9a\u6027\u548c\u58fd\u547d\u4e2d\u767c\u63ee\u95dc\u9375\u4f5c\u7528\u3002 Sir2 \u7684\u4eba\u985e\u540c\u6e90\u7269 SIRT1 \u4e5f\u5177\u6709 NAD(+) \u4f9d\u8cf4\u6027 p53 \u53bb\u4e59\u91af\u5316\u9176\u548c NAD(+) \u4f9d\u8cf4\u6027\u7d44\u86cb\u767d\u53bb\u4e59\u91af\u5316\u9176\u7684\u529f\u80fd\u3002 SIRT1 \u53ef\u8abf\u7bc0 p53 \u816b\u7624\u6291\u5236\u56e0\u5b50\u7684\u6d3b\u6027\u4e26\u6291\u5236\u7d30\u80de\u51cb\u4ea1\u3002\u9019\u4e9b\u7d50\u679c\u5c0d\u65bc SIRT1 \u5728\u8abf\u7bc0 p53 \u4f9d\u8cf4\u6027\u7d30\u80de\u51cb\u4ea1\u53cd\u61c9\u4e2d\u7d30\u80de\u654f\u611f\u6027\u7684\u91cd\u8981\u4f5c\u7528\u4ee5\u53ca\u764c\u75c7\u6cbb\u7642\u4e2d\u7684\u53ef\u80fd\u6548\u679c\u5177\u6709\u91cd\u8981\u610f\u7fa9\u3002\u7531\u65bc\u900f\u904e\u8207DNA\u640d\u50b7\u8a66\u5291\u4e00\u8d77\u4f7f\u7528\u4f7fp53\u7684\u529f\u80fd\u5f37\u529b\u589e\u5f37\uff0c\u56e0\u6b64SIRT1\u6291\u5236\u5291\u6709\u671b\u6210\u70ba\u6709\u6548\u7684\u6297\u764c\u85e5\u7269\u3002\u7136\u800c\uff0c\u6e2c\u91cfSIRT1\u6d3b\u6027\u7684\u5e38\u898f\u65b9\u6cd5\u975e\u5e38\u8907\u96dc\u4e14\u8cbb\u529b\u3002\u70ba\u4e86\u6e2c\u91cfSIRT1\u9176\u6d3b\u6027\uff0c\u9700\u8981\u88fd\u5099\u653e\u5c04\u6027\u4e59\u91af\u5316\u7d44\u86cb\u767d\u4f5c\u70ba\u5e95\u7269\u3002\u9996\u5148\uff0c\u5fc5\u9808\u900f\u904e\u5728\u57f9\u990a\u57fa\u4e2d\u6dfb\u52a0\u653e\u5c04\u6027\u4e59\u9178\u4f86\u7528\u653e\u5c04\u6027\u4ee3\u8b1d\u6a19\u8a18\u7d30\u80de\u3002\u5176\u6b21\uff0c\u5fc5\u9808\u5f9e\u7d30\u80de\u7d14\u5316\u653e\u5c04\u6027\u4e59\u91af\u5316\u7d44\u86cb\u767d\u3002\u53cd\u61c9\u5f8c\uff0c\u9700\u8981\u63d0\u53d6\u4e26\u5206\u96e2\u4e59\u91af\u5316\u7d44\u86cb\u767d\u4e2d\u91cb\u653e\u7684\u653e\u5c04\u6027\u4e59\u91af\u57fa\uff0c\u4f7f\u7528\u4e59\u9178\u4e59\u916f\u6839\u64da\u653e\u5c04\u6027\u6e2c\u91cf\u9176\u7684\u6d3b\u6027\u3002\u5118\u7ba1\u8fd1\u5e74\u4f86\u5831\u5c0e\u4e86\u4e0d\u4f7f\u7528\u653e\u5c04\u6027\u7269\u8cea\u6e2c\u91cf\u53bb\u4e59\u91af\u9176\u6d3b\u6027\u7684\u65b9\u6cd5\uff0c\u4f46\u7531\u65bc\u4f7f\u7528\u87a2\u5149\u6a19\u8a18\u7684\u4e59\u91af\u5316\u8cf4\u6c28\u9178\u4f5c\u70ba\u5e95\u7269\uff0c\u5fc5\u9808\u5c07\u53cd\u61c9\u7522\u7269\u8207\u5b8c\u6574\u7684\u5e95\u7269\u5206\u96e2\u4e26\u6e2c\u91cf\u87a2\u5149\u5f37\u5ea6\u4ee5\u53cd\u76f8HPLC \u3002\u5982\u4e0a\u6240\u8ff0\uff0c\u9019\u4e9b\u6e2c\u91cf\u7cfb\u7d71\u7531\u65bc\u64cd\u4f5c\u8907\u96dc\u800c\u96e3\u4ee5\u9069\u61c9\u5728\u5404\u7a2e\u689d\u4ef6\u4e0b\u8655\u7406\u8a31\u591a\u6a23\u54c1\u3002\u56e0\u6b64\uff0c\u512a\u9078\u7528\u65bc\u751f\u5316\u5206\u6790\u4ee5\u53ca\u4e0d\u4f7f\u7528\u653e\u5c04\u6027\u7269\u8cea\u7684\u6291\u5236\u5291\u7be9\u9078\u7684\u7c21\u55ae\u7cfb\u7d71\u3002<\/p>\n

Sirtuin \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/span><\/strong><\/a><\/span>\u00a0| SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/strong><\/a><\/span> | NMN<\/strong><\/a> | NAD+\/NADH \u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/a><\/span> | \u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/strong><\/span> | Metformin<\/a> | \u96f7\u5e15\u9ef4\u83cc\uff08Rapamycin\uff09<\/a><\/span><\/span><\/p>\n

SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(SIRT1 Activity Assay Kit)\u4e3b\u8981\u8a2d\u8a08\u7528\u65bc\u4f7f\u7528\u7c97 SIRT1 \u7d1a\u5206(crude SIRT1 fraction)\u6216\u7d14\u5316\u7684 SIRT1 \u5feb\u901f\u3001\u9748\u654f\u5730\u8a55\u4f30 SIRT1 \u6291\u5236\u5291\u6216\u6d3b\u5316\u5291\u3002\u6b64\u5916\uff0c\u5982\u679c\u4f7f\u7528\u9069\u7576\u7684\u91dd\u5c0d SIRT1 \u7684\u6297\u9ad4\uff08\u6297 SIRT1 \u6297\u9ad4 (ab7343)\uff09\u9032\u884c\u514d\u75ab\u6c89\u6fb1\uff0c\u5247\u53ef\u4ee5\u4f7f\u7528 SIRT1 \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u6e2c\u5b9a\u4efb\u4f55\u57f9\u990a\u7684\u539f\u4ee3\u7d30\u80de\u3001\u7d30\u80de\u7cfb\u6216\u7d44\u7e54\u52fb\u6f3f\u7684 SIRT1 \u6d3b\u6027\u3002Abcam \u7684 SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09\u5df2\u88ab\u8b49\u660e\u53ef\u4ee5\u6aa2\u6e2c Sirtuins \u7684\u6d3b\u6027\uff0c\u81f3\u5c11\u53ef\u4ee5\u6aa2\u6e2c\u4eba\u985e\u6216\u52d5\u7269\u7d30\u80de\u88c2\u89e3\u7269\u6216\u67f1\u7d1a\u5206\u4e2d\u7684 SIRT1\u3002\u6b64\u6e2c\u5b9a\u986f\u793a\u6a23\u54c1\u53cd\u61c9\u5177\u6709\u826f\u597d\u7684\u7dda\u6027\u3002\u6b64\u6e2c\u5b9a\u53ef\u7528\u65bc\u8ffd\u8e64Sirtuins\u7684\u7d14\u5316\u6216\u53ef\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u88c2\u89e3\u7269\u4e2dSirtuins\u7684\u5b58\u5728\u3002<\/p>\n

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1. \u76e3\u6e2cSIRT1\u7684\u7d14\u5316\u60c5\u6cc1\u3002<\/p>\n

2.\u7be9\u9078SIRT1\u7684\u6291\u5236\u5291\u6216\u6d3b\u5316\u5291\u3002<\/p>\n

3.\u6aa2\u6e2c\u85e5\u7269\u5c0dSIRT1\u7684\u5f71\u97ff\u3002<\/p>\n

<\/p>\n

\u529f\u80fd\u7814\u7a76 – SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09(ab156065)<\/strong><\/span><\/div>\n
C2C12 \u548c L6 \u6210\u808c\u7d30\u80de\u5206\u5225\u4ee5 1 \u548c 2 \u00b5M KL1333 \u8655\u7406 1 \u5c0f\u6642\u3002\u4f7f\u7528\u57fa\u65bc\u87a2\u5149\u7684\u6e2c\u5b9a\u6cd5\u5206\u6790\u4e86\u5169\u7a2e\u7d30\u80de\u7cfb\u4e2d\u7684 SIRT1 \u6d3b\u6027\u3002<\/div>\n
Seo\u3001Kang-Sik \u7b49\u4eba\uff0c\u795e\u7d93\u75c5\u5b78\u524d\u6cbf\uff1f\u5377\u30029 552.\uff0c\u57162\uff0cdoi\uff1a10.3389\/fneur.2018.00552<\/em><\/div>\n
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\u529f\u80fd\u7814\u7a76 – SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6e2c\u5b9a\uff09(ab156065)<\/strong><\/span><\/div>\n
\u50c5\u5728\u8f09\u9ad4\u5c0d\u7167\u548c NAM \u689d\u4ef6\u4e0b\uff0c\u4f7f\u7528 46 \u500b\u5be6\u9a57\u7d44\u7684 9 \u7d44\u7d30\u80de\u7684\u4ee3\u8868\u6027\u6a23\u672c\u6aa2\u6e2c\u7b2c 21 \u5929\u7684 SIRT1 \u9176\u6d3b\u6027<\/div>\n
Shapiro\uff0cAllison LB \u7b49\u4eba\uff0cPloS one\uff1fvol\u300211,7 e0159575.\uff0c\u5716 2\uff0cdoi:10.1371\/journal.pone.0159575<\/em><\/div>\n
<\/div>\n
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SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab156065)<\/strong><\/span><\/div>\n
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\u5728 EX-527 ( ab141506<\/a>\u00a0)\u5b58\u5728\u7684\u60c5\u6cc1\u4e0b\u91cd\u7d44 SIRT1 \u5c0d SIRT1 \u5e95\u7269\u53bb\u4e59\u91af\u5316\u7684\u6642\u9593\u904e\u7a0b<\/p>\n

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SIRT1 \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 (ab156065)<\/strong><\/span><\/div>\n
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\u91cd\u7d44 SIRT1 \u5c0d SIRT1 \u5e95\u7269\u53bb\u4e59\u91af\u5316\u7684\u6642\u9593\u904e\u7a0b<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

<\/p>\n

ab156065 – SIRT1 \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09<\/strong><\/span><\/div>\n
\u91cd\u7d44SIRT1\u6d3b\u6027\u7684\u5291\u91cf\u4f9d\u8cf4\u6027\u66f2\u7dda<\/div>\n
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ab156065 – SIRT1 \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09<\/span><\/div>\n
\n

\u6e2c\u91cf\u5148\u524d\u4ee5\u6297 SIRT1 \u6297\u9ad4\u514d\u75ab\u6c89\u6fb1\u7684\u6a23\u672c\u4e2d\u7684 293T \u7d30\u80de\u5167\u6e90\u6027 SIRT1 \u6d3b\u6027\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/strong><\/p>\n