{"id":45956,"date":"2023-10-05T15:52:34","date_gmt":"2023-10-05T07:52:34","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45956"},"modified":"2025-11-11T13:37:55","modified_gmt":"2025-11-11T05:37:55","slug":"arg2-%e6%85%a2%e7%97%85%e6%af%92%e8%bc%89%e9%ab%94%ef%bc%88%e4%ba%ba%e9%a1%9e%ef%bc%89%ef%bc%88cmv%ef%bc%89%ef%bc%88plenti-giii-cmv%ef%bc%89%e8%b2%a8%e8%99%9f12270061","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45956","title":{"rendered":"ARG2 \u6162\u75c5\u6bd2\u8f09\u9ad4\uff08\u4eba\u985e\uff09\uff08CMV\uff09\uff08pLenti-GIII-CMV\uff09\u8ca8\u865f12270061"},"content":{"rendered":"

ARG2 \u6162\u75c5\u6bd2\u8f09\u9ad4\uff08\u4eba\u985e\uff09\uff08CMV\uff09\uff08pLenti-GIII-CMV\uff09\u8ca8\u865f12270061<\/strong><\/a><\/span><\/p>\n

\u6162\u75c5\u6bd2\u7c21\u4ecb<\/b><\/span><\/p>\n

\u3000\u3000\u6162\u75c5\u6bd2\uff08Lentivirus\uff09\u7684\u7d50\u69cb\u53ef\u5206\u6210\u5957\u819c\uff08envelop\uff09\u3001\u86cb\u767d\u8cea\u819c\uff08capsid\uff09\u3001\u4ee5\u53ca\u907a\u50b3\u7269\u8cea\u3002Lentivirus\u611f\u67d3\u5bbf\u4e3b\u5f8c\uff0c\u6703\u5c07\u5176\u57fa\u56e0\u5d4c\u5165\u5bbf\u4e3b\u7d30\u80de\u4f7f\u5176\u8868\u9054\u3002\u73fe\u70ba\u5be6\u9a57\u5ba4\u4e2d\u5e38\u898b\u7684\u57fa\u56e0\u91cd\u7d44\u5de5\u5177\u3002<\/p>\n

\u3000\u3000\u76ee\u524dlentivirus\u5df2\u53ef\u85c9\u7531\u30001. \u5e36\u6709target gene\u7684lentivector<\/strong><\/span>\uff0c\u52a0\u4e0a\u30002. packaging system<\/strong><\/span>\uff0c\u69cb\u6210\u7121\u81f4\u75c5\u6027\u7684psuedovirus\u3002<\/p>\n

ABM Lentiviral Expression System \u6162\u75c5\u6bd2<\/b>\u8868\u73fe\u8f09\u9ad4<\/b><\/span><\/p>\n

    \n
  1. \u4e0d\u50c5\u53ef\u505a\u70ba\u75c5\u6bd2\u8f09\u9ad4\u4f7f\u7528\uff0c\u4ea6\u53ef\u505a\u70ba\u4e00\u822c\u8f09\u9ad4\u9032\u884c\u8f49\u67d3\u3002<\/li>\n
  2. \u5e36\u6709\u5169\u7a2e\u6297\u751f\u7d20\u7be9\u9078\u4f4d\uff0c\u4f9b\u539f\u6838\u6216\u771f\u6838\u751f\u7269\u4f7f\u7528\u3002<\/li>\n
  3. \u4e00\u7cfb\u5217\u5177\u6709\u4e0d\u540c\u555f\u52d5\u5b50\u7684\u6162\u75c5\u6bd2\u8f09\u9ad4\uff0c\u5982EF1a\u3001CMV\u3001UbC\u3001PGK\u3002\u4ea6\u6709\u5c08\u4f9bpromoter\u7814\u7a76\u4e4bpromoterless\u8f09\u9ad4\u3002EF1a \u555f\u52d5\u5b50(EF1a promoter)\u4f7f\u8868\u9054\u8f49\u57fa\u56e0\u7684\u7d30\u80de\u767e\u5206\u6bd4\u6700\u9ad8\uff0c\u5176\u6b21\u662f PGK \u548c UBC\u3002\u7136\u800c\uff0cPGK \u555f\u52d5\u5b50(PGK promoter) \u5728\u967d\u6027\u7d30\u80de\u4e2d\u63d0\u4f9b\u66f4\u9ad8\u7684\u57fa\u56e0\u8868\u73fe\u6c34\u5e73\u3002<\/li>\n
  4. \u53ef\u4f9d\u4e0d\u540c\u5be6\u9a57\u9700\u6c42\u4f7f\u7528bicistronic\u3001tricistronic\uff0c\u6216\u662f\u5e36\u6709reporter gene \uff08GFP\u3001RFP\u3001Luciferase\uff09\u4e4b\u75c5\u6bd2\u8f09\u9ad4\u3002<\/li>\n
  5. \u4f9d\u4e0d\u540c\u9700\u6c42\u751f\u7522\u5ba2\u88fd\u5316\u8f09\u9ad4\u3002<\/li>\n<\/ol>\n

    <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Cat. No.<\/td>\n12270061<\/span><\/td>\n<\/tr>\n
    Name<\/td>\nARG2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)<\/td>\n<\/tr>\n
    Unit<\/td>\n1.0 \u00b5g DNA<\/td>\n<\/tr>\n
    Gene Name<\/td>\nARG2<\/td>\n<\/tr>\n
    Gene Full Name<\/td>\narginase, type II<\/td>\n<\/tr>\n
    Accession Number<\/td>\nBC029050<\/td>\n<\/tr>\n
    Species<\/td>\nHuman<\/td>\n<\/tr>\n
    Vector Map<\/td>\npLenti-GIII-CMV\u00a0<\/a>(click blue link to view)<\/td>\n<\/tr>\n
    System<\/td>\nLentiviral Vector<\/td>\n<\/tr>\n
    Insert Size<\/td>\n1065<\/td>\n<\/tr>\n
    Vector Size<\/td>\n7684<\/td>\n<\/tr>\n
    Storage Condition<\/td>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
    <\/div>\n
    \n
    MOI = \u7522\u7269\u6ef4\u5ea6 (IU\/ml) x \u75c5\u6bd2\u9ad4\u7a4d (ml) \/ \u7d30\u80de\u7e3d\u6578<\/strong><\/span>
    \nMOI \u662f\u6307\u611f\u67d3\u4e2d\u4f7f\u7528\u7684\u6bcf\u500b\u7d30\u80de\u7684\u75c5\u6bd2\u9846\u7c92\u6578\u91cf\uff0c\u4f8b\u5982 MOI \u70ba 5 \u8868\u793a\u5b54\u4e2d\u6bcf\u500b\u7d30\u80de\u6709 5 \u500b\u611f\u67d3\u55ae\u4f4d (IU) \u6216\u8f49\u5c0e\u55ae\u4f4d (TU)\u3002\u8981\u78ba\u5b9a\u611f\u67d3\u8907\u6578 (MOI)\uff0c\u8acb\u8a08\u7b97\u6bcf\u500b\u5b54\u4e2d\u6dfb\u52a0\u7684\u75c5\u6bd2\u9846\u7c92\u6578\u91cf\uff0c\u7136\u5f8c\u5c07\u8a72\u6578\u5b57\u9664\u4ee5\u63a5\u7a2e\u5230\u5b54\u4e2d\u7684\u7d30\u80de\u6578\u91cf\u3002\u6211\u5011\u4e5f\u5efa\u8b70\u4f7f\u7528\u4e00\u7cfb\u5217 MOIs \u9032\u884c\u8f49\u5c0e\uff0c\u56e0\u70ba\u4e0d\u540c\u7684\u7d30\u80de\u985e\u578b\u53ef\u80fd\u9700\u8981\u4e0d\u540c\u7684 MOIs \u624d\u80fd\u6210\u529f\u8f49\u5c0e\u3002MOI = \u75c5\u6bd2\u6ef4\u5ea6 (IU\/ml) x \u75c5\u6bd2\u9ad4\u7a4d (ml) \/ \u7d30\u80de\u7e3d\u6578<\/p>\n
    \n
    \n

    SV40 \u6b63\u5411\u5f15\u5b50\u5e8f\u5217 5′ ACTGAGGGGCCTGAAATGA SV40 \u53cd\u5411\u5f15\u5b50\u5e8f\u5217 5′ GACTCAGGGCATGAAACAGG \u9019\u4e9b\u662f qPCR \u5f15\u7269\uff0c\u689d\u5e36\u5927\u5c0f\u70ba 61 bp\u3002<\/p>\n<\/div>\n<\/div>\n

    \u5f71\u97ff\u6162\u75c5\u6bd2\u5305\u88dd\u529b\u50f9\u7684\u56e0\u7d20\u6709\u5f88\u591a\uff0c\u5305\u62ec\u63d2\u5165\u7247\u6bb5\u5927\u5c0f( insert size)\u3001\u63d2\u5165\u7247\u6bb5\u5e8f\u5217(insert sequence)\uff08specific sequence\/\u7279\u5b9a\u5e8f\u5217\uff09\u4ee5\u53ca\u7528\u65bc\u75c5\u6bd2\u8f09\u9ad4\u751f\u7522\u7684\u83cc\u682a\u3002\u6c92\u6709\u4eba\u53ef\u4ee5\u78ba\u5b9a\u54ea\u7a2e\u63d2\u5165\u7269\u6548\u679c\u66f4\u597d\u3002\u8f49\u5c0e(Transduction)\u53ef\u4ee5\u5177\u6709\u66f4\u9ad8\u7684\u8f49\u5c0e\u6548\u7387\u548c\u66f4\u9ad8\u6c34\u5e73\u7684\u57fa\u56e0\u8868\u9054\uff0c\u56e0\u70ba\u53ef\u4ee5\u8d85\u904e100\u500b\u62f7\u8c9d\u7684DNA\u9032\u5165\u7d30\u80de\u3002\u4f46\u53ea\u6709\u7d04 1% \u7684\u8f49\u5c0e\u7d30\u80de\u6703\u6574\u5408\u5230\u5bbf\u4e3b\u7d30\u80de\u4e2d\u3002\u7531\u65bc\u53ea\u6709 1-10 \u500b\u62f7\u8c9d\u7684\u75c5\u6bd2\u9846\u7c92\u9032\u5165\u7d30\u80de\uff0c\u6162\u75c5\u6bd2\u6548\u7387\u901a\u5e38\u4f4e\u65bc\u8f49\u67d3\uff0c\u8868\u9054\u91cf\u4e5f\u4f4e\u65bc\u8f49\u67d3\u8868\u9054\u3002\u6b64\u5916\uff0c\u6548\u7387\u4e5f\u4f9d\u8cf4\u7d30\u80de\u682a\u3002\u6211\u5011\u5df2\u7d93\u5145\u5206\u8b49\u660e\u6162\u75c5\u6bd2\u53ef\u4ee5\u8f15\u9b06\u8f49\u5c0e 293 \u548c MDA-MB-468 \u7d30\u80de\u3002\u8acb\u6e2c\u8a66\u9019\u5169\u7a2e\u7d30\u80de\u682a\uff0c\u4ee5\u78ba\u5b9a\u662f\u5426\u662f\u529b\u50f9\u554f\u984c\uff0c\u6216\u8005\u53ef\u80fd\u662f\u8a72\u7279\u5b9a\u7d30\u80de\u682a\u96e3\u4ee5\u88ab\u6162\u75c5\u6bd2\u8f49\u5c0e\u3002<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

    ARG2 \u6162\u75c5\u6bd2\u8f09\u9ad4\uff08\u4eba\u985e\uff09\uff08CMV\uff09\uff08pLenti-GIII-CMV\uff09\u8ca8\u865f12270061 \u6162\u75c5\u6bd2\u7c21\u4ecb \u3000\u3000 […]<\/p>\n","protected":false},"author":3,"featured_media":29552,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,7021],"tags":[6117],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45956"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45956"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45956\/revisions"}],"predecessor-version":[{"id":45960,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45956\/revisions\/45960"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29552"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45956"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45956"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}