{"id":45883,"date":"2023-09-26T17:45:04","date_gmt":"2023-09-26T09:45:04","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45883"},"modified":"2023-10-22T12:06:54","modified_gmt":"2023-10-22T04:06:54","slug":"%e8%b1%ac%e8%83%b0%e5%b3%b6%e7%b4%a0%e6%a8%a3%e7%94%9f%e9%95%b7%e5%9b%a0%e5%ad%90-2%ef%bc%8cigf-2-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-porcine-insulin-like-growth-factors-2-igf-2-elisa-kit%e8%b2%a8","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45883","title":{"rendered":"\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2\uff0cIGF-2 ELISA \u8a66\u5291\u76d2 | Porcine Insulin-like Growth Factors 2, IGF-2 ELISA Kit\u8ca8\u865fE0285Po"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=29125\"><span style=\"color: #33cccc;\"><strong><span style=\"font-size: 12pt;\">\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2\uff0cIGF-2 ELISA \u8a66\u5291\u76d2 | Porcine Insulin-like Growth Factors 2, IGF-2 ELISA Kit\u8ca8\u865fE0285Po<\/span><\/strong><\/span><\/a><\/span><\/p>\n<p>Insulin-like Growth Factors 2 (IGF-2) (0.3-90ng\/ml)<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.bt-laboratory.com\/webui\/shop\/pc\/images\/nopic_kit.jpg\" alt=\"\" \/><\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a<span style=\"text-decoration: underline;\">\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2<\/span><\/strong><span style=\"text-decoration: underline;\">\u00a0<\/span>ELISA\u8a66\u5291\u76d2 (Insulin-like Growth Factors 2 (IGF-2) ) \u7528\u65bc\u5b9a\u6027(Qualitative)\u6a23\u54c1\u4e2d\u7684\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2 (IGF-2) \u3002\u6e2c\u91cf\u7269\u7a2e: Porcine \u3002\u8a72\u8a66\u5291\u76d2\u57fa\u65bc\u5b9a\u6027\u53cd\u76f8\u9176\u514d\u75ab\u5206\u6790\u6280\u8853(reverse phase enzyme immuno assay technique)\u3002 \u5fae\u91cf\u6ef4\u5b9a\u677f\u5df2\u9810\u5148\u5857\u6709\u76ee\u6a19\u6297\u9ad4\u3002 \u5c07\u967d\u6027\/\u9670\u6027\u5c0d\u7167\u6216\u6a23\u54c1\u52a0\u5165\u5b54\u4e2d\u4e26\u5b75\u80b2\u3002 \u6a23\u54c1\u4e2d\u7684\u6297\u539f\u8207\u677f\u4e0a\u7684\u6297\u9ad4\u7d50\u5408\u3002 \u672a\u7d50\u5408\u7684\u6297\u539f\u5728\u6d17\u6ecc\u6b65\u9a5f\u4e2d\u88ab\u6d17\u6389\u3002 \u7136\u5f8c\u52a0\u5165\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 (Horseradish Peroxidase , HRP) \u5076\u806f\u7684\u6aa2\u6e2c\u6297\u9ad4\u4e26\u5b75\u80b2\u3002 \u672a\u7d50\u5408\u7684 HRP \u5728\u6d17\u6ecc\u6b65\u9a5f\u4e2d\u88ab\u6d17\u6389\u3002 \u7136\u5f8c\u52a0\u5165 TMB \u5e95\u7269\u4e26\u986f\u8272\u3002 \u901a\u904e\u52a0\u5165\u9178\u6027\u7d42\u6b62\u6eb6\u6db2\u7d42\u6b62\u53cd\u61c9\uff0c\u984f\u8272\u8b8a\u70ba\u9ec3\u8272\uff0c\u53ef\u5728 450 nm \u8655\u6e2c\u91cf\u3002 \u7136\u5f8c\u53ef\u4ee5\u5c07\u672a\u77e5\u6a23\u54c1\u7684 OD \u8207\u967d\u6027\u548c\u9670\u6027\u5c0d\u7167\u7684 OD \u9032\u884c\u6bd4\u8f03\uff0c\u4ee5\u78ba\u5b9a<strong><span style=\"text-decoration: underline;\">\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2<\/span><\/strong>\u7684\u5b58\u5728\u3002\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Serum, Plasma, Cell culture supernatant<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/09\/1-\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50-2\uff0cIGF-2-ELISA-\u8a66\u5291\u76d2-Porcine-Insulin-like-Growth-Factors-2-IGF-2-ELISA-Kit\u8ca8\u865fE0285Po.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-29126 alignleft\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/09\/BT-LAB-150x150.png\" alt=\"BT LAB\" width=\"174\" height=\"198\" \/><\/p>\n<p><strong>Product Summary<\/strong><\/p>\n<p>Size:\u00a096 Test<\/p>\n<p>Assay type: Qualitative (0.3-90ng\/ml)<\/p>\n<p>Sample type: Serum, plasma, cell culture supernates<\/p>\n<p>Reactive with: Porcine<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/www.bt-laboratory.com\/webui\/shop\/pc\/images\/logo.png\" alt=\"\" width=\"147\" height=\"50\" \/><\/p>\n<p>Shanghai Crystal Day Biotech\u6210\u7acb\u65bc2009\u5e74\uff0c\u524d\u8eab\u70baShanghai Korain Biotech\uff0c\u8207\u5927\u5b78\u5be6\u9a57\u5ba4\u5408\u4f5c\uff0c\u65e8\u5728\u70ba\u5be6\u9a57\u5ba4\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u512a\u8cea\u3001\u5be6\u60e0\u7684ELISA\u5de5\u5177\u3002 \u7d93\u904e\u4e94\u5e74\u7684\u7814\u7a76\u548c\u6539\u9032\uff0cShanghai Korain Biotech\u6210\u70baELISA\u8a66\u5291\u76d2\u7684\u5c08\u6709\u5e73\u53f0\uff0c\u4e26\u5275\u7acb\u4e86\u201cbt-laboratory\uff08\u7c21\u7a31BT LAB\uff09\u201d\u54c1\u724c\uff0c\u64c1\u67096, 000+ ELISA\u8a66\u5291\u76d2\u7684\u8c50\u5bcc\u7522\u54c1\u7dda \u5305\u62ec\u593e\u5fc3ELISA\uff08\u9176\u806f\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\uff09\u8a66\u5291\u76d2\u3001Competitive EIA\uff08\u9176\u514d\u75ab\u6e2c\u5b9a\uff09\u8a66\u5291\u76d2\u3002 2018 \u5e74\uff0cBT LAB \u63a8\u51fa\u4e86\u64c1\u6709 10,000+ \u591a\u7a2e\u6297\u9ad4\u7684\u6297\u9ad4\u7cfb\u5217\u53ca10,000+ ELISA\u5957\u7d44\u30022019\u5e7410\u6708\uff0c\u6211\u5011\u7684\u5be6\u9a57\u5ba4\u548c\u5009\u5eab\u5728\u6d59\u6c5f\u5609\u8208\u5efa\u6210\u3002 \u73fe\u5728 Korain \u5728 5 \u5927\u6d32\u7684 35 \u500b\u570b\u5bb6\/\u5730\u5340\u767c\u5c55\u58ef\u5927\uff0c\u5f15\u7528\u6b21\u6578\u8d85\u904e 6,000+\u3002<\/p>\n<p><strong><span style=\"color: #800000; font-size: 12pt;\">\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57 (enzyme-linked immunosorbent assay , ELISA)\u539f\u7406<\/span><\/strong><\/p>\n<p>\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57 (enzyme-linked immunosorbent assay , ELISA) \u662f\u4e00\u7a2e\u5e38\u7528\u7684\u5206\u6790\u751f\u5316\u5206\u6790\u65b9\u6cd5(biochemistry assay)\u3002 \u9019\u6e2c\u5b9a\u4f7f\u7528\u56fa\u76f8\u985e\u578b\u7684\u9176\u514d\u75ab\u6e2c\u5b9a(enzyme immunoassay)\u6aa2\u6e2c\u6db2\u9ad4\u6a23\u54c1\u4e2d\u86cb\u767d\u8cea\u7684\u5b58\u5728\u4f7f\u7528\u91dd\u5c0d\u86cb\u767d\u8cea\u7684\u6297\u9ad4\u6e2c\u91cf\u3002 \u5728\u6700\u7c21\u55ae\u7684 ELISA \u5f62\u5f0f\u4e2d\uff0c\u6297\u539f\u5f9e\u6a23\u54c1\uff08\u5982\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\u3001\u7d30\u80de\u88c2\u89e3\u6db2\u3001\u553e\u6db2\u3001\u7d44\u7e54\u88c2\u89e3\u6db2\u548c\u5c3f\u6db2\uff09\u88ab\u6e2c\u8a66\u7684\u7269\u9ad4\u9644\u8457\u5728\u4e00\u500b\u8868\u9762\u4e0a\u3002 \u7136\u5f8c\uff0c\u4e00\u500b\u5339\u914d\u7684\u6297\u9ad4\u88ab\u5857\u5728\u8868\u9762\u4e0a\uff0c\u6240\u4ee5\u5b83\u53ef\u4ee5\u7d50\u5408\u6297\u539f\u3002 \u9019\u7a2e\u6297\u9ad4\u8207\u9176\u76f8\u9023\u7136\u5f8c\u53bb\u9664\u4efb\u4f55\u672a\u7d50\u5408\u7684\u6297\u9ad4\u3002 \u5728\u6700\u5f8c\u4e00\u6b65\uff0c\u542b\u6709\u9176\u7684\u7269\u8cea\u6dfb\u52a0\u5e95\u7269\u3002 \u5982\u679c\u6709\u7d81\u5b9a\u5f8c\u7e8c\u53cd\u61c9\u7522\u751f\u53ef\u6aa2\u6e2c\u7684\u4fe1\u865f\uff0c\u6700\u5e38\u898b\u7684\u662f\u984f\u8272\u8b8a\u5316\u3002<\/p>\n<p>High sensitivity and specificity ELISA kit Sensitivity of the assay is the smallest detectable amount of the target protein in question, it depends mainly on the affinity of the solid phase antibody according to the law of mass action. Specificity helps us recognize if the antibody detect the target protein specifically without crossreacting with non-pecific proteins. Monoclonal antibodies have very high affinity for the antigen, which also have higher specificity than polyclonal antibodies. A monoclonal antibody is used as the detecting antibody in BT LAB sandwich ELISA to provide improved specificity. \u2022 Extensive analyte selection \u2022 Strict QC control \u2022 Vadidated sample testing<\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u9ad8\u9748\u654f\u5ea6(sensitivity)\u548c\u7279\u7570\u6027(specificity )ELISA\u8a66\u5291\u76d2<\/span><\/strong><\/span><\/p>\n<p>\u6e2c\u5b9a\u7684\u9748\u654f\u5ea6(sensitivity)\u662f\u6240\u8a0e\u8ad6\u7684\u76ee\u6a19\u86cb\u767d\u8cea\u7684\u6700\u5c0f\u53ef\u6aa2\u6e2c\u91cf\uff0c\u5b83\u6839\u64da\u8cea\u91cf\u4f5c\u7528\u5b9a\u5f8b\uff0c\u4e3b\u8981\u53d6\u6c7a\u65bc\u56fa\u76f8\u6297\u9ad4\u7684\u89aa\u548c\u529b\u3002\u7279\u7570\u6027\u5e6b\u52a9\u6211\u5011\u8b58\u5225\u6297\u9ad4\u662f\u5426\u7279\u7570\u6027\u6aa2\u6e2c\u76ee\u6a19\u86cb\u767d\u8cea\uff0c\u800c\u4e0d\u6703\u8207\u975e\u7279\u7570\u6027\u86cb\u767d\u8cea\u767c\u751f\u4ea4\u53c9\u53cd\u61c9\u3002 \u55ae\u514b\u9686\u6297\u9ad4\u5c0d\u6297\u539f\u6709\u5f88\u9ad8\u7684\u89aa\u548c\u529b\uff0c\u5176\u7279\u7570\u6027\u4e5f\u9ad8\u65bc\u591a\u514b\u9686\u6297\u9ad4\u3002 \u55ae\u514b\u9686\u6297\u9ad4\u7528\u4f5cBT LAB \u593e\u5fc3 ELISA \u4e2d\u7684\u6aa2\u6e2c\u6297\u9ad4\u53ef\u63d0\u4f9b\u66f4\u9ad8\u7684\u7279\u7570\u6027\u3002<\/p>\n<ul>\n<li>\u5ee3\u6cdb\u7684\u5206\u6790\u7269\u9078\u64c7<\/li>\n<li>\u56b4\u683c\u7684\u8cea\u91cf\u63a7\u5236<\/li>\n<li>\u9a57\u8b49\u6a23\u672c\u6e2c\u8a66<\/li>\n<\/ul>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>\u7cbe\u5ea6\u548c\u518d\u73fe\u6027Precision &amp; Reproducibility<\/strong><\/span><\/p>\n<p>\u7cbe\u5bc6\u5ea6(Precision)\u662f\u4f5c\u70ba\u5e73\u5747\u503c\u7684\u8b8a\u7570\u4fc2\u6578 (variation , CV) \u4f86\u8861\u91cf\u7684\u3002CV \u61c9\u6839\u64da\u8a08\u7b97\u51fa\u7684\u6fc3\u5ea6\u800c\u4e0d\u662f\u539f\u59cb\u5149\u5b78\u503c\u4f86\u8a08\u7b97\u5bc6\u5ea6\u3002<\/p>\n<ul>\n<li>\u5c0f\u65bc15 \u7684\u5206\u6790\u9593% CV \u901a\u5e38\u662f\u53ef\u4ee5\u63a5\u53d7\u7684\u3002<\/li>\n<li>\u6aa2\u6e2c\u5167 % CV \u61c9\u5c0f\u65bc 10\u3002<\/li>\n<li>\u5831\u544a\u53cd\u6620\u7d50\u679c\u7bc4\u570d\u7684\u6fc3\u5ea6\u7684 CV \u5f88\u91cd\u8981\u6a19\u672c\u4e2d\u767c\u73fe\u3002<\/li>\n<\/ul>\n<p>BT Lab ELISA Types There are several ELISA configurations, with the most common formats being sandwich, competitive and signaling assays. Concentration-dependent detection of the target analyte is accompanied by either an increase or a decrease in signal, which is related to the format that is chosen.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8c6c\u80f0\u5cf6\u7d20\u6a23\u751f\u9577\u56e0\u5b50 2\uff0cIGF-2 ELISA \u8a66\u5291\u76d2 | Porcine Insulin-like Grow [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":29126,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6088],"tags":[6132,6131],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45883"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45883"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45883\/revisions"}],"predecessor-version":[{"id":45897,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45883\/revisions\/45897"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29126"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45883"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45883"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45883"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}