{"id":45848,"date":"2023-09-25T17:37:46","date_gmt":"2023-09-25T09:37:46","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45848"},"modified":"2023-12-12T16:09:50","modified_gmt":"2023-12-12T08:09:50","slug":"%e8%bc%94%e9%85%b6-%e2%85%a1-nadph-%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-micro-coenzyme-%e2%85%a1-nadph-assay-kit%e8%b2%a8%e8%99%9fktb1010","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45848","title":{"rendered":"\u8f14\u9176 \u2161 NADP(H) \u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Coenzyme \u2161 NADP(H) Assay Kit\u8ca8\u865fKTB1010"},"content":{"rendered":"

\u8f14\u9176 \u2161 NADP(H) \u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Coenzyme \u2161 NADP(H) Assay Kit\u8ca8\u865fKTB1010<\/span><\/strong><\/a><\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d0.5 \u00b5M-10 \u00b5M<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>
\n<\/span><\/span><\/p>\n

\u7159\u91af\u80fa\u817a\u560c\u5464\u4e8c\u6838\u82f7\u9178\u78f7\u9178 (Nicotinamide adenine dinucleotide phosphate, NADP) \u662f\u4e00\u7a2e\u9175\u7d20\u8f14\u52a9\u56e0\u5b50(enzymatic cofactor)\uff0c\u53c3\u8207\u8a31\u591a\u6c27\u5316\u9084\u539f\u53cd\u61c9(redox reactions )\uff0c\u5728\u9084\u539f ( reduced , NADPH) \u548c\u6c27\u5316 (oxidized , NADP) \u5f62\u5f0f\u4e4b\u9593\u5faa\u74b0\u3002 NADP \u4e5f\u53c3\u8207\u751f\u7269\u5408\u6210\u53cd\u61c9\uff0c\u4f8b\u5982\u8102\u8cea( lipid )\u548c\u6838\u9178\u5408\u6210(nucleic acid synthesis )\uff0c\u5176\u4e2d\u5b83\u5145\u7576\u9084\u539f\u5291( reducing agent)\u3002\u78f7\u9178\u620a\u7cd6\u9014\u5f91 (pentose phosphate pathway , PPP) \u7684\u6c27\u5316\u5206\u652f( oxidative branch)\u662f\u52d5\u7269\u7d30\u80de\u4e2d\u7522\u751f\u7684 NADPH \u7684\u4e3b\u8981\u4f86\u6e90\u3002\u4eba\u5011\u4e00\u76f4\u5c0d\u76e3\u6e2c\u5176\u6fc3\u5ea6\u6c34\u5e73\u611f\u8208\u8da3\u3002 NADP+ \/NADPH \u7684\u5b9a\u91cf\u6e2c\u5b9a\u53ef\u61c9\u7528\u65bc\u7d30\u80de\u6216\u7d44\u7e54\u7684\u80fd\u91cf\u8f49\u5316\u548c\u6c27\u5316\u9084\u539f\u72c0\u614b\u7684\u7814\u7a76\u3002\u8f14\u9176 \u2161 NADP(H) \u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u9748\u654f\u6aa2\u6e2c NADP \u548c NADPH \u53ca\u5176\u5728\u5404\u7a2e\u7d44\u7e54\u548c\u4e9e\u7d30\u80de\u5668(subcellular organelles) \u4e2d\u7684\u6bd4\u4f8b\u63d0\u4f9b\u4e86\u4fbf\u6377\u7684\u5de5\u5177\u3002\u6b64\u8a66\u5291\u76d2\u57fa\u65bc\u9175\u7d20\u5faa\u74b0\u53cd\u61c9\uff08\u4e0d\u8b58\u5225 NAD+\/NADH\uff09\uff0c\u5176\u4e2d\u5f62\u6210\u7684NADPH reduces a formazan (MTT) \u8a66\u5291\u3002\u5728 OD565 nm \u8655\u6e2c\u91cf\u7684\u9084\u539f\u7522\u7269\u984f\u8272\u7684\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u7684 NADP+ \/NADPH \u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002<\/p>\n

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<\/p>\n

<\/p>\n

\u6587\u737b\u5f15\u7528 Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells<\/a><\/span><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\n
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\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Coenzyme \u2161 NADP(H) Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine CheKine\u2122 Micro Coenzyme \u2161 NADP(H) Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+\/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ \/NADPH concentration in the sample.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Assay Buffer
\n\u2022 Glucose (1 M)
\n\u2022 WST-8 Solution
\n\u2022 Enhancer Solution
\n\u2022 NADP Cycling Enzyme Mix
\n\u2022 NADPH standard (10 mM)
\n\u2022 NADP Extraction Buffer
\n\u2022 NADPH Extraction Buffer<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Detect NADP+\/NADPH concentrations and ratios in cell or tissue extracts.
\n\u2022 Detection limit of 0.1 \u00b5M and linearity up to 4 \u00b5M NADP+\/NADPH in 96-well plate assay.<\/td>\n<\/tr>\n
Calibration range<\/strong><\/td>\n0.5 \u00b5M-10 \u00b5M<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Addition of Working Reagent should be quick and mixing should be brief but thorough.
\n\u2022 The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at \u201320\u00b0C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nNicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ \/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Image & description<\/span><\/h3>\n
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\"Fig.<\/div>\n

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is \u0394OD and the x-axis is NADPH concentration (uM).<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\u5f15\u7528\u6587\u737b<\/strong><\/span><\/p>\n

Apelin, Omentin-1, and Vaspin in patients with essential hypertension: association of adipokines with trace elements, inflammatory cytokines, and oxidative damage markers(KTB2130-1.1)<\/p>\n

Author\uff1aSerinkan Cinemre FB, Cinemre H, Bahtiyar N, Kahyao\u011flu B, A\u011fa\u00e7 MT, Shundo H, Sevin\u00e7 L, Aydemir B Publication name\uff1aIr J Med Sci IF\uff1a1.1<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8f14\u9176 \u2161 NADP(H) \u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Coenzyme \u2161 NADP(H) […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[6135],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45848"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45848"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45848\/revisions"}],"predecessor-version":[{"id":47831,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45848\/revisions\/47831"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45848"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45848"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45848"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}