{"id":45822,"date":"2023-09-25T14:07:11","date_gmt":"2023-09-25T06:07:11","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45822"},"modified":"2023-10-22T12:08:01","modified_gmt":"2023-10-22T04:08:01","slug":"%e8%84%ab%e8%9b%8b%e7%99%bd%e6%a8%a3%e5%93%81%e8%a3%bd%e5%82%99%e8%a9%a6%e5%8a%91%e7%9b%92-deproteinizing-sample-preparation-kit%e8%b2%a8%e8%99%9fpi-0103","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45822","title":{"rendered":"\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 | Total Carbohydrate Assay\u8ca8\u865fPI-0103\u00a0"},"content":{"rendered":"

\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 <\/span><\/strong>| Deproteinizing Sample Preparation Kit<\/strong>\u8ca8\u865fPI-0103<\/strong><\/span><\/a>\u00a0<\/span><\/span><\/p>\n

Deproteinizing Sample Preparation Kit<\/em><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u86cb\u767d\u8cea\u548c\u5404\u7a2e\u9176\u6d3b\u6027\u7684\u5b58\u5728\u7d93\u5e38\u5e72\u64fe\u751f\u7269\u6a23\u54c1\u4e2d\u5c0f\u5206\u5b50\u7684\u5206\u6790\u3002\u8a31\u591a\u751f\u7269\u6e2c\u5b9a\u9700\u8981\u5728\u5206\u6790\u524d\u5f9e\u6a23\u54c1\u4e2d\u53bb\u9664\u86cb\u767d\u8cea\u3002\u751f\u7269\u5316\u5b78\u53ca\u76f8\u95dc\u9818\u57df\u7684\u57fa\u672c\u8981\u6c42\u662f\u80fd\u5920\u53bb\u9664\u6240\u7814\u7a76\u6a23\u54c1\u4e2d\u7684\u90a3\u4e9b\u6703\u4ee5\u591a\u7a2e\u65b9\u5f0f\uff08\u7269\u7406\u548c\u5316\u5b78\uff09\u65b9\u5f0f\u5e72\u64fe\u5206\u6790\u7684\u6210\u5206\u3002\u7576\u6240\u8a0e\u8ad6\u7684\u5206\u6790\u7269\u662f\u5c0f\u5206\u5b50\u6642\uff0c\u86cb\u767d\u8cea\u3001\u6838\u9178\u548c\u78b3\u6c34\u5316\u5408\u7269\u7b49\u5927\u5206\u5b50\u7684\u5b58\u5728\u6703\u56b4\u91cd\u640d\u5bb3\u5206\u6790\u3002\u5728\u904e\u53bb\u534a\u500b\u4e16\u7d00\u958b\u767c\u7684\u812b\u86cb\u767d\u65b9\u6848\u4e2d\uff0c\u9ad8\u6c2f\u9178\uff08PCA\uff09\u6c89\u6fb1\u5df2\u7d93\u5ee3\u6cdb\u61c9\u7528\u65bc\u8a31\u591a\u4e0d\u540c\u7684\u6a23\u54c1\u88fd\u5099\u7a0b\u5e8f\uff0c\u56e0\u70ba\u5b83\u4e0d\u50c5\u53ef\u4ee5\u53bb\u9664\u5927\u90e8\u5206\u5b58\u5728\u7684\u86cb\u767d\u8cea\uff0c\u800c\u4e14\u9084\u53ef\u4ee5\u7a69\u5b9a\u8a31\u591a\u5c0f\u5206\u5b50\u5206\u6790\u7269\u3002\u901a\u5e38\u4f7f\u7528\u4e19\u916e(acetone)\u3001\u9ad8\u6c2f\u9178 (perchloric acid, PCA)\u3001\u504f\u78f7\u9178 (metaphosphoric acid, MPA)\u3001\u78fa\u57fa\u6c34\u694a\u9178 (sulfosalicylic acid, SSA) \u6216\u4e09\u6c2f\u4e59\u9178 (trichloroacetic acid, TCA) \u7b49\u5316\u5b78\u7269\u8cea\u4e4b\u4e00\u900f\u904e\u6c89\u6fb1\u53bb\u9664\u86cb\u767d\u8cea\u3002PCA\u53bb\u86cb\u767d\u5316\u65b9\u6cd5\u5df2\u7d93\u6210\u529f\u61c9\u7528\u65bc\u5b9a\u91cf\u5c0f\u5206\u5b50\u9663\u5217\uff08\u5305\u62ec\u7cd6\u539f\uff0cATP\uff0ccAMP\uff0c\u7a40\u80f1\u7518\u80bd\uff0c\u6297\u6c27\u5316\u5291\u7b49\uff09\u4e4b\u524d\u7684\u6e96\u5099\u6a23\u54c1\u4e2d\u3002\u65b0\u8a2d\u8a08\u7684\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2(Deproteinizing Sample Preparation Kit) <\/em><\/strong>\u5229\u7528\u9ad8\u6c2f\u9178 (perchloric acid, PCA)\u6c89\u6fb1\u6cd5\uff0c\u63d0\u4f9b\u7368\u7279\u7684\u5de5\u5177\u6e96\u5099\u6a23\u54c1\u7528\u65bc\u5206\u6790\u5404\u7a2e\u5c0f\u5206\u5b50\u3002\u9664\u86cb\u767d\u8cea\u6c89\u6fb1\u5916\uff0c\u53bb\u9664\u591a\u9918\u7684\u9ad8\u6c2f\u9178 (perchloric acid, PCA)\uff0c\u6a23\u54c1\u6700\u5f8c\u88ab\u4e2d\u548c\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u88fd\u5099\u7684\u6a23\u54c1\u53ef\u76f4\u63a5\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6e2c\u5b9a\u3002\u8a72\u65b9\u6cd5\u65b9\u4fbf\uff0c\u65b9\u4fbf\uff0c\u53ef\u7528\u65bc\u88fd\u5099\u5927\u91cf\u6a23\u54c1\u3002<\/p>\n

<\/p>\n

\u5716\uff1a\u8840\u6e05\u6a23\u672c\u7684\u53bb\u86cb\u767d\u3002\u4f9d\u7167\u8a66\u5291\u76d2\u65b9\u6848\uff0c\u4f7f\u7528\u4e0d\u540c\u91cf\u7684 PCA \u5c0d 400 \u00b5l \u8840\u6e05\u9032\u884c\u53bb\u86cb\u767d\u5316\u3002\u6e2c\u91cf\u6eb6\u6db2\u4e2d\u5269\u9918\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6 OD280 (\u2666) \u548c\u6a23\u54c1 pH (\u2022)\u3002\u5c07\u6a23\u672c\uff08\u7d14\u8840\u6e05\uff1b\u900f\u904e 10kD MW \u622a\u6b62\u904e\u6ffe\u5668\u904e\u6ffe\u4e26\u7d93\u904e PCA \u8655\u7406\u7684\u8840\u6e05\uff09\u7a00\u91cb 20 \u500d\uff0c\u4ee5\u4fbf\u65bc\u6e2c\u91cf 280 nm \u7684 OD\u3002\u5c07\u76f8\u540c PCA \u8655\u7406\u7684\u6a23\u54c1\u7684\u55ae\u7368\u7b49\u5206\u90e8\u5206\u8207 pH 7.75 \u6e2c\u5b9a\u7de9\u885d\u6db2\u4ee5 1:1 \u6df7\u5408\uff0c\u4e26\u6e2c\u5b9a\u6df7\u5408\u7269\u7684 pH \u503c\u3002\u6b64\u5716\u986f\u793a\uff0c\u5728\u6240\u5f97\u6aa2\u6e2c pH \u503c\u4fdd\u6301\u5728\u76ee\u6a19\u6aa2\u6e2c pH \u503c\u7d04 0.5 \u500b pH \u55ae\u4f4d\u7684\u7bc4\u570d\u5167\u6642\uff0c\u53ef\u4ee5\u5bb9\u5fcd\u9ad8\u9054 20% \u7684\u6a23\u54c1\/PCA \u6bd4\u7387\u8aa4\u5dee\u3002\u4f7f\u7528 10 kDa MW \u622a\u6b62\u904e\u6ffe\u5668\u53ef\u53bb\u9664\u7d04 98% \u7684\u86cb\u767d\u8cea\uff0c\u800c PCA\/\u6a23\u672c\u6bd4\u4f8b\u70ba 1:4 \u6642\u7684 PCA \u6c89\u6fb1\u7269\u53ef\u53bb\u9664\u7d04 95% \u7684\u86cb\u767d\u8cea\u3002<\/p>\n\n\n\n\n\n\n
Components<\/th>\nIdentifier<\/th>\n200 tests<\/th>\n<\/tr>\n<\/thead>\n
Neutralization Solution<\/td>\nNM<\/td>\n1 x 4ml<\/td>\n<\/tr>\n
PCA<\/td>\nWM<\/td>\n1 x 20ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n

PRODUCT SUMMARY<\/span><\/strong><\/span><\/h3>\n<\/div>\n
\n
\n

Catalog\u00a0<\/strong>PI-0103<\/p>\n

Size<\/strong>\u00a0Includes reagents for 200 preparations<\/p>\n

Sample Type\u00a0<\/strong>Cell Lysate, Tissue Extracts, Serum, Urine<\/p>\n

Species\u00a0<\/strong>Non specific<\/p>\n

Assay Type\u00a0<\/strong>Qualitative<\/p>\n

Application\u00a0<\/strong>To remove proteins from\u00a0various\u00a0sample\u00a0types.<\/p>\n

Storage Conditions\u00a0<\/strong>Room Temperature<\/p>\n

Shipping Temperature\u00a0<\/strong>Room Temperature<\/p>\n

Shelf Life\u00a0<\/strong>One year from the date of delivery<\/p>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u65b9\u6848 (Deproteinizing Sample Preparation Protocol)<\/span><\/strong><\/span><\/p>\n

This procedure may be scaled to handle a wide range of sample sizes.<\/p>\n

1. \u86cb\u767d\u8cea\u6c89\u6fb1(Protein Precipitation) : For samples with a protein concentration < ~20 mg\/ml (tissue homogenate, cell lysate, urine, etc.), to 500 \u00b5l of sample, add 100 \u00b5l of ice cold PCA in a 1.5 ml microfuge tube, vortex and place on ice for 5 min. Centrifuge at 13,000 x g for 2 min. Transfer precisely 480 \u00b5l of the supernatant to a fresh tube. For serum and other samples with a high protein concentration, to 400 \u00b5l of sample, add 100 \u00b5l of ice-cold PCA, place on ice for 5 min. Centrifuge at 13,000 x g for 2 min. Transfer precisely 380 \u00b5l of the supernatant to a fresh tube. Depending on the nature of the analyte, PCA treated samples may be stored at -70\u00b0C for up to a month.\u6839\u64da\u5206\u6790\u7269\u7684\u6027\u8cea\uff0cPCA \u8655\u7406\u7684\u6a23\u54c1\u53ef\u5728 -70\u00b0C \u4e0b\u4fdd\u5b58\u9577\u9054\u4e00\u500b\u6708\u3002<\/p>\n

2. \u6a23\u672c\u4e2d\u548c(Sample Neutralization): To the clear supernatant obtained after centrifugation, add 20 \u00b5l of shaken ice-cold Neutralization Solution and vortex briefly to neutralize the sample and precipitate excess PCA. There will be some gas (CO2) evolution so vent the sample tube. Place on ice for 5 min then spin briefly. Samples are now deproteinized, neutralized, and PCA has been removed. The samples may now be used in a variety of assays directly.\u77ed\u66ab\u6e26\u65cb\u4ee5\u4e2d\u548c\u6a23\u672c\u4e26\u6c89\u6fb1\u904e\u91cf\u7684 PCA\u3002\u5c07\u6703\u7522\u751f\u4e00\u4e9b\u6c23\u9ad4 (CO2)\uff0c\u56e0\u6b64\u8acb\u5c0d\u6a23\u54c1\u7ba1\u9032\u884c\u6392\u6c23\u3002\u7f6e\u65bc\u51b0\u4e0a 5 \u5206\u9418\uff0c\u7136\u5f8c\u77ed\u66ab\u65cb\u8f49\u3002\u6a23\u54c1\u73fe\u5df2\u88ab\u812b\u86cb\u767d\u3001\u4e2d\u548c\uff0c\u4e26\u4e14 PCA \u5df2\u88ab\u53bb\u9664\u3002\u9019\u4e9b\u6a23\u54c1\u73fe\u5728\u53ef\u4ee5\u76f4\u63a5\u7528\u65bc\u5404\u7a2e\u6e2c\u5b9a\u3002<\/p>\n

<\/p>\n

AkrivisBio\u54c1\u724c\u4ecb\u7d39<\/strong><\/span><\/p>\n

AkrivisBio \u662f\u751f\u547d\u79d1\u5b78\u548c\u751f\u7269\u6280\u8853\u7522\u696d\u7814\u7a76\u7522\u54c1\u7684\u63d0\u4f9b\u8005\u3002 \u300cAkrivis\u300d\u4f86\u81ea\u5e0c\u81d8\u8a9e\uff0c\u610f\u601d\u662f\u7cbe\u78ba\uff0c\u96c6\u4e2d\u9ad4\u73fe\u4e86\u6211\u5011\u7684\u76ee\u6a19\u3001Bio\u3001\u6211\u5011\u7684\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u59cb\u65bc\u5404\u7a2e\u5316\u5b78\u3001\u751f\u5316\u548c\u9175\u7d20\u6d3b\u6027\u6e2c\u5b9a\u3002\u6211\u5011\u8a2d\u8a08\u5b83\u5011\u6bd4\u5176\u4ed6\u516c\u53f8\u63d0\u4f9b\u7684\u7522\u54c1\u66f4\u5bb9\u6613\u4f7f\u7528\u3001\u66f4\u5177\u53ef\u91cd\u8907\u6027\u4e14\u66f4\u4fbf\u5b9c\u3002\u6211\u5011\u5e0c\u671b\u770b\u5230\u6211\u5011\u7684\u6aa2\u6e2c\u65b9\u6cd5\u5728\u5c0b\u627e\u764c\u75c7\u3001\u7cd6\u5c3f\u75c5\u3001\u80a5\u80d6\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u548c\u5176\u4ed6\u9818\u57df\u89e3\u6c7a\u65b9\u6848\u7684\u751f\u5316\u7814\u7a76\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u6211\u5011\u52aa\u529b\u53ca\u6642\u5411\u5ba2\u6236\u63d0\u4f9b\u6700\u597d\u7684\u7522\u54c1\u3002\u6211\u5011\u76f8\u4fe1\u5353\u8d8a\u3001\u52e4\u596e\u3001\u5275\u9020\u529b\u548c\u627f\u8afe\u3002\u6211\u5011\u5c0d\u79d1\u5b78\u5145\u6eff\u71b1\u60c5\uff0c\u81f4\u529b\u65bc\u70ba\u5ba2\u6236\u63d0\u4f9b\u6709\u50f9\u503c\u7684\u7522\u54c1\u3001\u5c08\u5bb6\u5efa\u8b70\u548c\u7121\u53ef\u6311\u5254\u7684\u5ba2\u6236\u652f\u63f4\u3002\u6211\u5011\u64c1\u6709\u5168\u7403\u7d93\u92b7\u5546\u7db2\u8def\u3002<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u812b\u86cb\u767d\u6a23\u54c1\u88fd\u5099\u8a66\u5291\u76d2 | Deproteinizing Sample Preparation Kit\u8ca8\u865fPI- […]<\/p>\n","protected":false},"author":3,"featured_media":45835,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6087],"tags":[6137],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45822"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45822"}],"version-history":[{"count":7,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45822\/revisions"}],"predecessor-version":[{"id":45872,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45822\/revisions\/45872"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/45835"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45822"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45822"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45822"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}