{"id":45807,"date":"2023-09-25T11:27:48","date_gmt":"2023-09-25T03:27:48","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45807"},"modified":"2023-10-22T12:08:14","modified_gmt":"2023-10-22T04:08:14","slug":"atp%e5%91%88%e8%89%b2%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-atp-colorimetric-fluorometric-assay-kit-%e8%b2%a8%e8%99%9fma-0113","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45807","title":{"rendered":"ATP\u5448\u8272\u5206\u6790\u5957\u7d44 | ATP Colorimetric\/Fluorometric Assay Kit \u8ca8\u865fMA-0113"},"content":{"rendered":"

\u00a0<\/strong>A<\/strong>T<\/strong>P<\/strong>\u5448\u8272\u5206\u6790\u5957\u7d44<\/strong>ATP Colorimetric\/Fluorometric Assay Kit<\/strong> | MA-0113<\/strong><\/a><\/span><\/p>\n

<\/p>\n

\u7522\u54c1\u4ecb\u7d39: akrivisbio ATP<\/strong>\u5448\u8272\u87a2\u5149<\/strong>\u5206\u6790\u5957\u7d44<\/strong>\uff1b\u63d0\u4f9b\u4e86\u5448\u8272\u548c\u87a2\u5149\u5169\u65b9\u5f0f\uff0c\u6e2c\u91cfATP\u542b\u91cf\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0cATP\u8f49\u5316\u70baADP\u548c\u4e19\u916e\u9178\u3002\u7522\u751f\u7684\u4e19\u916e\u9178\u9e7d\u53ef\u4ee5\u901a\u904e\u6bd4\u8272\u6cd5\uff08\u03bbmax = 570nm\uff09\u6216\u87a2\u5149\u6cd5\uff08Ex \/ Em 535 \/ 587nm\uff09\u5b9a\u91cf\u3002ATP \u662f\u751f\u547d\u7cfb\u7d71\u7684\u4e3b\u8981\u80fd\u91cf\u3002\u4e8b\u5be6\u4e0a\uff0c\u6240\u6709\u4f9d\u8cf4\u80fd\u91cf\u7684\u904e\u7a0b\u90fd\u5229\u7528\u5132\u5b58\u5728ATP \u7684\u78f7\u9178\u9375(phosphate bond)\u3002 ATP \u50c5\u5728\u7c92\u7dda\u9ad4( mitochondria )\u4e2d\u5f62\u6210\uff0c\u591a\u7a2e\u907a\u50b3\u75be\u75c5\u6703\u5f71\u97ff\u7c92\u7dda\u9ad4\u4e2d ATP \u7684\u5f62\u6210\u3002\u6709\u8a31\u591a\u5e02\u552e ATP \u6aa2\u6e2c\u65b9\u6cd5\u53ef\u4f7f\u7528\u51b7\u5149(luminescence)\u65b9\u6cd5\u4f86\u6aa2\u6e2c\u98db\u6469\u723e\u6216\u66f4\u5c11\u7684 ATP\uff0c\u87a2\u5149\u7d20\u9176(luciferase)\u7cfb\u7d71\u4e0d\u7a69\u5b9a\uff0c\u4e26\u4e14\u5728\u5927\u591a\u6578\u5be6\u9a57\u5ba4\u4e2d\u901a\u5e38\u4e0d\u63d0\u4f9b\u51b7\u5149\u8a2d\u5099\u3002 akrivisbio\u65b0\u8a2d\u8a08\u7684 ATP \u6bd4\u8272\u6cd5\u548c\u87a2\u5149\u6cd5\u6aa2\u6e2c\u8a66\u5291\u76d2\u88ab\u8a2d\u8a08\u70ba\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u7a69\u5b9a\u548c\u9ad8\u901a\u91cf\u9069\u61c9\u6027\uff0c\u5229\u7528\u7518\u6cb9( glycerol)\u7684\u78f7\u9178\u5316\u4f86\u7522\u751f\u6613\u65bc\u5b9a\u91cf\u7684\u7522\u7269\uff0c\u900f\u904e\u6bd4\u8272\u6cd5 (OD 570 nm) \u6216\u87a2\u5149\u6cd5 (Ex\/Em = 535\/587 nm) \u65b9\u6cd5\u3002\u6b64\u6e2c\u5b9a\u53ef\u6aa2\u6e2c\u5404\u7a2e\u7269\u8cea\u4e2d\u4f4e\u81f3 50 pmol (1 \u00b5M) \u7684 ATP\u6a23\u54c1\u3002\u6b64\u8a66\u5291\u76d2\u63d0\u4f9b\u8db3\u5920 100 \u6b21\u6aa2\u6e2c\u7684\u8a66\u5291\u3002<\/p>\n

\u6e2c\u5b9a\u539f\u7406 (Assay Principle)<\/span><\/strong><\/span><\/p>\n

1 \u2013 \u6a23\u54c1\u4e2d\u7684 ATP \u7528\u65bc\u78f7\u9178\u5316\u7518\u6cb9<\/p>\n

2 \u2013 \u78f7\u9178\u7518\u6cb9\u88ab\u6c27\u5316\uff0c\u5f62\u6210\u904e\u6c27\u5316\u6c2b<\/p>\n

3 \u2013 \u904e\u6c27\u5316\u7269\u9176\u5229\u7528\u904e\u6c27\u5316\u6c2b\u6c27\u5316 ADHP\uff0c\u5f62\u6210\u5f37\u70c8\u7684\u984f\u8272\u548c\u87a2\u5149<\/p>\n

\n

PRODUCT SUMMARY<\/span><\/strong><\/span><\/h3>\n<\/div>\n
\n
\n

Catalog\u00a0<\/strong>MA-0113<\/p>\n

Size<\/strong>100 wells<\/p>\n

Sample Type\u00a0\u00a0<\/strong>Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, other biological fluids<\/p>\n

Species\u00a0<\/strong>All<\/p>\n

Method of Detection\u00a0<\/strong>Colorimetric, OD 570\u00a0nm or Fluorometric, Ex\/Em = 535\/587\u00a0nm<\/p>\n

Assay Type\u00a0<\/strong>Quantitative<\/p>\n

Application<\/strong>A\u00a0plate based colorimetric\/fluorometric assay\u00a0to\u00a0quantify the amount of ATP present in various samples. The assay can detect down to 50 pmol (1 \u00b5M) of ATP.<\/em><\/p>\n

Storage Conditions<\/strong>-20\u00b0C<\/p>\n

Shipping Temperature\u00a0<\/strong>Gel Pack<\/p>\n

Shelf Life\u00a0<\/strong>One year from the date of delivery<\/p>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

1. Sample Preparation: Cells (106
\n) or tissues (10 mg) are homogenized or otherwise disrupted quickly in 100 \u00b5l of a protein precipitant (TCA,
\nPCA) on ice then centrifuged and the clear supernatant transferred to another tube. See details on protein removal in PI-0102 or PI-0103
\ndatasheet. Transfer 2-10 \u00b5l of sample to a 96-well plate. Adjust all well volumes to 50 \u00b5l Assay Buffer. To correct for endogenous background,
\nprepare each sample in pairs with one of the pair used as a non-ATP control.
\n1. \u6a23\u54c1\u88fd\u5099(Sample Preparation)\uff1a\u7d30\u80de (106<\/sup>\uff09\u6216\u7d44\u7e54\uff0810 mg\uff09\u5728 100 \u00b5l \u86cb\u767d\u8cea\u6c89\u6fb1\u5291\uff08TCA\uff0cPCA\uff09\u7f6e\u65bc\u51b0\u4e0a\uff0c\u7136\u5f8c\u96e2\u5fc3\uff0c\u5c07\u6f84\u6e05\u7684\u4e0a\u6e05\u6db2\u8f49\u79fb\u81f3\u53e6\u4e00\u7ba1\u4e2d\u3002\u8acb\u53c3\u95b1 PI-0102 \u6216 PI-0103 \u4e2d\u6709\u95dc\u86cb\u767d\u8cea\u53bb\u9664\u7684\u8a73\u7d30\u4fe1\u606f\u6578\u64da\u8868\u3002\u5c07 2-10 \u00b5l \u6a23\u54c1\u8f49\u79fb\u81f3 96 \u5b54\u677f\u3002\u5c07\u6240\u6709\u5b54\u9ad4\u7a4d\u8abf\u6574\u70ba 50 \u00b5l \u6e2c\u5b9a\u7de9\u885d\u6db2\u3002\u70ba\u4e86\u6821\u6b63\u5167\u6e90\u6027\u80cc\u666f\uff0c\u6210\u5c0d\u6e96\u5099\u6bcf\u500b\u6a23\u54c1\uff0c\u5176\u4e2d\u4e00\u500b\u6a23\u54c1\u7528\u4f5c\u975e ATP \u5c0d\u7167\u3002<\/p>\n

<\/p>\n

AkrivisBio\u54c1\u724c\u4ecb\u7d39<\/strong><\/span><\/p>\n

AkrivisBio \u662f\u751f\u547d\u79d1\u5b78\u548c\u751f\u7269\u6280\u8853\u7522\u696d\u7814\u7a76\u7522\u54c1\u7684\u63d0\u4f9b\u8005\u3002 \u300cAkrivis\u300d\u4f86\u81ea\u5e0c\u81d8\u8a9e\uff0c\u610f\u601d\u662f\u7cbe\u78ba\uff0c\u96c6\u4e2d\u9ad4\u73fe\u4e86\u6211\u5011\u7684\u76ee\u6a19\u3001Bio\u3001\u6211\u5011\u7684\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u59cb\u65bc\u5404\u7a2e\u5316\u5b78\u3001\u751f\u5316\u548c\u9175\u7d20\u6d3b\u6027\u6e2c\u5b9a\u3002\u6211\u5011\u8a2d\u8a08\u5b83\u5011\u6bd4\u5176\u4ed6\u516c\u53f8\u63d0\u4f9b\u7684\u7522\u54c1\u66f4\u5bb9\u6613\u4f7f\u7528\u3001\u66f4\u5177\u53ef\u91cd\u8907\u6027\u4e14\u66f4\u4fbf\u5b9c\u3002\u6211\u5011\u5e0c\u671b\u770b\u5230\u6211\u5011\u7684\u6aa2\u6e2c\u65b9\u6cd5\u5728\u5c0b\u627e\u764c\u75c7\u3001\u7cd6\u5c3f\u75c5\u3001\u80a5\u80d6\u3001\u795e\u7d93\u9000\u5316\u6027\u75be\u75c5\u548c\u5176\u4ed6\u9818\u57df\u89e3\u6c7a\u65b9\u6848\u7684\u751f\u5316\u7814\u7a76\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u6211\u5011\u52aa\u529b\u53ca\u6642\u5411\u5ba2\u6236\u63d0\u4f9b\u6700\u597d\u7684\u7522\u54c1\u3002\u6211\u5011\u76f8\u4fe1\u5353\u8d8a\u3001\u52e4\u596e\u3001\u5275\u9020\u529b\u548c\u627f\u8afe\u3002\u6211\u5011\u5c0d\u79d1\u5b78\u5145\u6eff\u71b1\u60c5\uff0c\u81f4\u529b\u65bc\u70ba\u5ba2\u6236\u63d0\u4f9b\u6709\u50f9\u503c\u7684\u7522\u54c1\u3001\u5c08\u5bb6\u5efa\u8b70\u548c\u7121\u53ef\u6311\u5254\u7684\u5ba2\u6236\u652f\u63f4\u3002\u6211\u5011\u64c1\u6709\u5168\u7403\u7d93\u92b7\u5546\u7db2\u8def\u3002<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

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