{"id":45789,"date":"2023-09-22T16:05:50","date_gmt":"2023-09-22T08:05:50","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45789"},"modified":"2023-10-22T12:09:44","modified_gmt":"2023-10-22T04:09:44","slug":"2-nbdg-%e8%91%a1%e8%90%84%e7%b3%96%e6%94%9d%e5%8f%96%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-2-nbdg-glucose-uptake-assay-kit-%e8%b2%a8%e8%99%9fabnova-ka6077","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45789","title":{"rendered":"2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| 2-NBDG Glucose Uptake Assay Kit \u8ca8\u865fAbnova\u2122 KA6077"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><strong><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=45789&amp;preview=true\"><span style=\"font-size: 12pt;\">2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| 2-NBDG Glucose Uptake Assay Kit \u8ca8\u865fAbnova\u2122 KA6077<\/span><\/a><\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/abnova.png\" width=\"628\" height=\"82\" \/><\/p>\n<p><span style=\"color: #999999;\">200 assays\u00a0<\/span><\/p>\n<p>2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6aa2\u6e2c\u8a66\u5291\u76d2(2-NBDG Glucose Uptake Assay Kit )\u63d0\u4f9b\u4e86\u4e00\u7a2e\u9748\u654f\u7684\u975e\u653e\u5c04\u6027\u6aa2\u6e2c\u65b9\u6cd5(non-radioactive assay)\uff0c\u7528\u65bc\u6e2c\u91cf\u57f9\u990a\u7d30\u80de\u4e2d\u7684\u8461\u8404\u7cd6\u651d\u53d6(glucose uptake)\u3002\u87a2\u5149\u8a0a\u865f\u53ef\u4ee5\u900f\u904e\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6709488 nm\u96f7\u5c04\u548c530\/30 nm\u767c\u5c04\u6ffe\u5149\u7247\uff08FITC\u901a\u9053\uff09\u7684\u6d41\u5f0f\u7d30\u80de\u5100\u4f86\u76e3\u6e2c\u3002\u6b64\u6aa2\u6e2c\u8a66\u5291\u76d2\u662f\u76e3\u6e2c\u8461\u8404\u7cd6\u8f49\u904b\u86cb\u767d(monitoring glucose transporters)\u7684\u6700\u5f37\u5927\u7684\u5de5\u5177\u3002\u8461\u8404\u7cd6\u4ee3\u8b1d(Glucose metabolism)\u662f\u5c07\u8461\u8404\u7cd6(glucose)\u8f49\u5316\u70ba\u80fd\u91cf\u7684\u904e\u7a0b\uff0c\u4e5f\u662f\u5927\u591a\u6578\u751f\u7269\u9ad4\u80fd\u91cf\u4f9b\u61c9\u7684\u4e3b\u8981\u4f86\u6e90\u3002 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose] \u662f\u4e00\u7a2e\u87a2\u5149\u6a19\u8a18\u7684\u8461\u8404\u7cd6\u793a\u8e64\u5291(glucose tracer)\uff0c\u5df2\u88ab\u8b49\u660e\u53ef\u4ee5\u6709\u6548\u76e3\u6e2c\u8461\u8404\u7cd6\u8f49\u904b( glucose transportation)\u5728\u7d30\u80de\u4e2d\uff0c2-NBDG \u900f\u904e\u8207\u8461\u8404\u7cd6\u76f8\u540c\u7684\u8461\u8404\u7cd6\u8f49\u904b\u86cb\u767d (GLUT) \u8f49\u904b\u5230\u7d30\u80de\u4e2d\u3002\u4e00\u65e6 2-NBDG \u88ab\u7d30\u80de\u651d\u53d6\uff0c\u5b83\u5c31\u6703\u5728 C-6 \u4f4d\u9ede\u78f7\u9178\u5316( phosphorylation )\uff0c\u7522\u751f 2-NBDG-6-\u78f7\u9178\u9e7d(2-NBDG-6-phosphate)\uff0c\u4e26\u4e14\u5f88\u597d\u5730\u4fdd\u7559\u5728\u7d30\u80de\u5167\u3002\u8207\u5176\u4ed6\u8461\u8404\u7cd6\u793a\u8e64\u5291\uff08\u4f8b\u5982 2-DG \u6216 FDG\uff09\u76f8\u6bd4\uff0c2-NBDG \u5141\u8a31\u5728\u55ae\u7d30\u80de\u5c64\u7d1a\u4ee5\u9ad8\u6642\u9593\u548c\u7a7a\u9593\u5206\u8fa8\u7387(spatial resolution)\u539f\u4f4d\u6e2c\u91cf 2-NBDG\u3002<\/p>\n<p>2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2(The 2-NBDG Glucose Uptake Assay Kit)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u9748\u654f\u7684\u975e\u653e\u5c04\u6027\u6e2c\u5b9a\u65b9\u6cd5(non-radioactive assay)\uff0c\u7528\u65bc\u6e2c\u91cf\u57f9\u990a\u7d30\u80de\u4e2d\u7684\u8461\u8404\u7cd6\u651d\u53d6\u3002\u5728\u9019\u500b\u8a66\u5291\u76d2\u4e2d\uff0cAssay Buffer I \u7528\u65bc\u589e\u5f37\u7d30\u80de\u4e2d 2-NBDG \u7684\u651d\u53d6( uptake )\u548c\u4fdd\u7559\uff0c\u800c Assay Buffer II \u53ef\u4ee5\u63d0\u9ad8\u7d30\u80de\u4e2d 2-NBDG \u7684\u8a0a\u865f\u80cc\u666f\u6bd4( signal-to-background ratio)\u3002\u87a2\u5149\u8a0a\u865f\u53ef\u4ee5\u900f\u904e\u87a2\u5149\u986f\u5fae\u93e1(fluorescence microscope )\u6216\u6709488 nm\u96f7\u5c04\u548c530\/30 nm\u767c\u5c04\u6ffe\u5149\u7247\uff08FITC\u901a\u9053\uff09\u7684\u6d41\u5f0f\u7d30\u80de\u5100\u4f86\u76e3\u6e2c\u3002 2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6aa2\u6e2c\u8a66\u5291\u76d2(The 2-NBDG Glucose Uptake Assay Kit)\u662f\u76e3\u6e2c\u8461\u8404\u7cd6\u8f49\u904b\u86cb\u767d\u6700\u5f37\u5927\u7684\u5de5\u5177\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/09\/5-2-NBDG-%E8%91%A1%E8%90%84%E7%B3%96%E6%94%9D%E5%8F%96%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%922-NBDG-Glucose-Uptake-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA6077.png\" \/><\/p>\n<p>Summary<\/p>\n<p>1. Prepare cells with your test compounds<\/p>\n<p>2. Add 2-NBDG staining solution<\/p>\n<p>3. Incubate cells at 37\u00b0C for 20 minutes<\/p>\n<p>4. Remove 2-NBDG staining solution<\/p>\n<p>5. Wash cells with Assay Buffer I<\/p>\n<p>6. Analyze cells using fluorescence microscope or flow cytometer with 530\/30 nm filter (FITC channel)<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/09\/1-2-NBDG-\u8461\u8404\u7cd6\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2-2-NBDG-Glucose-Uptake-Assay-Kit-\u8ca8\u865fAbnova\u2122-KA6077.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/09\/3-2-NBDG-%E8%91%A1%E8%90%84%E7%B3%96%E6%94%9D%E5%8F%96%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-2-NBDG-Glucose-Uptake-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA6077.jpg\" \/><\/p>\n<p>\u4f7f\u7528 2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6aa2\u6e2c\u8a66\u5291\u76d2\u5c0d CHO-K1 \u7d30\u80de\u7684 2-NBDG \u651d\u53d6\u9032\u884c\u6d41\u5f0f\u7d30\u80de\u5100\u3002 CHO-K1 \u7d30\u80de\u5728 37\u00b0C \u4e0b\u7528\u6216\u4e0d\u7528 100 uM \u6839\u76ae\u7d20\u8655\u7406 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u7528 100 uM 2-NBDG \u67d3\u8272\u6eb6\u6db2\u5b75\u80b2 20 \u5206\u9418\u3002\u70ba\u4e86\u6e96\u5099\u7528\u65bc\u6d41\u5f0f\u7d30\u80de\u5100\u7684\u8cbc\u58c1 CHO-K1 \u7d30\u80de\uff0c\u67d3\u8272\u5f8c\u4f7f\u7528 EDTA \u5206\u96e2\u7d30\u80de\u3002\u4f7f\u7528 ACEA NovoCyte \u6d41\u5f0f\u7d30\u80de\u5100\u5728 FITC \u901a\u9053\u4e2d\u6e2c\u91cf\u87a2\u5149\u5f37\u5ea6\u3002<\/p>\n<p>Example Data Analysis and Figures.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/09\/4-2-NBDG-%E8%91%A1%E8%90%84%E7%B3%96%E6%94%9D%E5%8F%96%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-2-NBDG-Glucose-Uptake-Assay-Kit-%E8%B2%A8%E8%99%9FAbnova%E2%84%A2-KA6077.jpg\" \/><br \/>\n\u4f7f\u7528 2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6aa2\u6e2c\u8a66\u5291\u76d2\u62cd\u651d CHO-K1 \u7d30\u80de\u4e2d 2-NBDG \u651d\u53d6\u7684\u87a2\u5149\u5f71\u50cf\u3002\u5c07 CHO-K1 \u7d30\u80de\u4ee5 40,000 \u500b\u7d30\u80de\/\u5b54\/100 uL \u63a5\u7a2e\u65bc 96 \u5b54\u9ed1\u58c1\/\u900f\u660e\u5e95\u677f\u4e2d\u904e\u591c\u3002\u5c07\u7d30\u80de\u4ee5 20 mM \u8461\u8404\u7cd6 (B) \u6216 100 uM \u6839\u76ae\u7d20 (C) \u5728 37\u00b0C \u4e0b\u8655\u7406 1 \u5c0f\u6642\uff0c\u7136\u5f8c\u8207 100 uM 2-NBDG \u67d3\u8272\u6eb6\u6db2\u4e00\u8d77\u5b75\u80b2 20 \u5206\u9418\u3002\u672a\u8655\u7406\u7684\u5c0d\u7167\u7d30\u80de\u5728\u76f8\u540c\u689d\u4ef6\u4e0b\u67d3\u8272\u3002\u4f7f\u7528 FITC \u6ffe\u5149\u7247\u7684\u87a2\u5149\u986f\u5fae\u93e1\u6e2c\u91cf\u87a2\u5149\u8a0a\u865f\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>2-NBDG \u8461\u8404\u7cd6\u651d\u53d6\u6e2c\u5b9a\u8a66\u5291\u76d2| 2-NBDG Glucose Uptake Assay Kit \u8ca8\u865fAb [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6146],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45789"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45789"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45789\/revisions"}],"predecessor-version":[{"id":45793,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45789\/revisions\/45793"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45789"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45789"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45789"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}