{"id":45557,"date":"2023-08-31T12:30:35","date_gmt":"2023-08-31T04:30:35","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45557"},"modified":"2023-10-22T12:18:56","modified_gmt":"2023-10-22T04:18:56","slug":"%e6%bc%86%e9%85%b6%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-laccase-activity-assay-kit-ak0319","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45557","title":{"rendered":"\u6f06\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2| Laccase Activity Assay Kit-AK0319"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=45302&amp;preview=true\"><strong><span style=\"font-size: 12pt;\">\u6f06\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2| Laccase Activity Assay Kit-AK0319<\/span><\/strong><\/a><\/span><\/p>\n<p>Laccase Activity Assay Kit-AK0319 (Spectrophotometer, 50T\/48S)<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6e2c\u5b9a\u610f\u7fa9\uff1a<\/span><\/strong><\/p>\n<p>\u6f06\u9176\uff08Caschen, Ce1.10.3.2\uff09\u662f\u4e00\u7a2e\u542b\u9285\u591a\u915a\u6c27\u5316\u9176(copper-containing polyphenol oxidase)\uff0c\u5c6c\u65bc\u9285\u85cd\u6c27\u5316\u7269\u5bb6\u65cf(copper blue oxide family)\uff0c\u5ee3\u6cdb\u5206\u4f48\u65bc\u771f\u83cc( fungi)\u548c\u9ad8\u7b49\u690d\u7269( plants)\u4e2d\uff0c\u6709\u5f88\u5f37\u7684\u6c27\u5316\u9084\u539f\u80fd\u529b\uff0c\u5728pulp bioreboxia\u4e2d\u5177\u6709\u5ee3\u6cdb\u7684\u61c9\u7528\u3001\u74b0\u5883\u6c61\u67d3\u7269\u964d\u89e3(environmental pollutant degradation )\u548c\u6728\u8cea\u7e96\u7dad\u7d20\u964d\u89e3(lignocellulose degradation)\u53ca\u751f\u7269\u6e2c\u5b9a\u3002\u6e2c\u5b9a\u539f\u7406\uff1aCaschen\u5206\u89e3\u200b\u200b\u7522\u7269ABTS\u7522\u751fABTS\u81ea\u7531\u57fa\uff0c420 nm\u8655\u7684\u5438\u5149\u5ea6\u4fc2\u6578\u9060\u5927\u65bc\u5e95\u7269ABTS\uff0c\u53ef\u8a08\u7b97\u51faABTS\u81ea\u7531\u57fa\u7684\u751f\u9577\u901f\u7387\u3002\u53ef\u4ee5\u8a08\u7b97\u6f06\u9176\uff08Caschen) \u7684\u6d3b\u6027\u3002<br \/>\nShentian \u5be6\u9a57\u7528\u54c1\u53ca\u5100\u5668\uff1a\u5929\u5e73(<strong>balance)<\/strong>\u3001\u4f4e\u6eab\u96e2\u5fc3\u6a5f(<strong>low temperature centrifuge)<\/strong>\u3001\u53ef\u898b\u5206\u5149\u5149\u5ea6\u8a08(<strong>visible spectrophotometer)<\/strong>\u30011ml\u73bb\u7483\u6bd4\u8272\u76bf\u3001\u6046\u6eab\u6c34\u6d74\u934b\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Components:<\/strong><\/span><br \/>\nComponents: Extract reagent: Liquid 60 mL\u00d71, store at 4\u2103;<br \/>\nReagent I: Liquid 50 mL\u00d71, store at 4\u2103;<br \/>\nReagent II: Powder\u00d72, store at 4\u2103 and protect from light.<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u9700\u8981\u4f46\u672a\u63d0\u4f9b\u7684\u8a66\u5291\u548c\u8a2d\u5099<\/strong><\/span><br \/>\nSpectrophotometer, balance, low temperature desk centrifuge, transferpettor, oscillating instrument, 1 mL glass cuvette, mortar\/ homogenizer, water bath, ice and distilled water.\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u64cd\u4f5c\u6d41\u7a0b-\u6a23\u54c1\u88fd\u5099<\/strong><\/span><\/p>\n<p>(1)\u7d44\u7e54\uff1a\u6309\u7167\u8cea\u91cf\uff08g\uff09\uff1a\u63d0\u53d6\u9ad4\u7a4d\uff08mL\uff09\uff1a1\uff1a5-10\u7684\u6bd4\u4f8b\u52a0\u5165\u63d0\u53d6\u8a66\u5291\u3002\u5efa\u8b70\u5c07 1 mL \u63d0\u53d6\u7269\u6dfb\u52a0\u5230 0.1 g \u7d44\u7e54\u4e2d\u3002\u5728\u51b0\u4e0a\u52fb\u6f3f\u3002 10000 g 4\u2103\u96e2\u5fc310\u5206\u9418\u3002\u53d6\u4e0a\u6e05\u7f6e\u65bc\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002 (2)\u7d30\u80de\uff1a\u6309\u7d30\u80de\u6578(10<sup>4<\/sup>)\uff1a\u63d0\u53d6\u6db2\u9ad4\u7a4d(mL)\u70ba500~1000:1\u3002\u5efa\u8b70500 million\u7d30\u80de\u6dfb\u52a01 mL\u63d0\u53d6\u8a66\u5291\u3002\u8d85\u8072\u6ce2\u7834\u788e\u7d30\u80de\u51b0\u6d74\uff08\u529f\u7387300W\uff0c\u8d85\u80723s\uff0c\u9593\u96947s\uff0c\u7e3d\u6642\u95933min\uff09\u3002 10000 g 4\u2103\u96e2\u5fc310\u5206\u9418\u3002\u53d6\u4e0a\u6e05\u7f6e\u65bc\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002 (3)\u57f9\u990a\u57fa\uff1a\u76f4\u63a5\u6aa2\u6e2c\u3002<\/p>\n<p><span style=\"color: #515a6e;\"><span style=\"font-size: 12pt; color: #800000;\"><strong>\u64cd\u4f5c\u6d41\u7a0b<\/strong><\/span><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/2.-%E6%BC%86%E9%85%B6%E6%B4%BB%E6%80%A7%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Laccase-Activity-Assay-Kit-AK0319.png\" width=\"836\" height=\"556\" \/><\/strong><\/p>\n<p>\u55ae\u4f4d\u5b9a\u7fa9\uff1a1\u500b\u9176\u55ae\u4f4d\u5b9a\u7fa9\u70ba\u6bcf10<sup>4<\/sup>\u500b\u7d30\u80de\u6bcf\u5206\u9418\u53cd\u61c9\u9ad4\u7cfb\u4e2d\u6c27\u53161nmol ABTS\u7684\u9176\u91cf\u3002Unit definition: One unit of enzyme is defined as the amount of enzyme oxidation of 1 nmol ABTS in the reaction system per minute every 10<sup>4<\/sup> cells.<\/p>\n<p><span style=\"color: #515a6e;\"><span style=\"font-size: 12pt; color: #800000;\"><strong>\u64cd\u4f5c\u6d41\u7a0b-\u7d50\u679c\u8a08\u7b97<\/strong><\/span><br \/>\n<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/3-%E6%BC%86%E9%85%B6%E6%B4%BB%E6%80%A7%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Laccase-Activity-Assay-Kit-AK0319.png\" width=\"729\" height=\"391\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/4-%E6%BC%86%E9%85%B6%E6%B4%BB%E6%80%A7%E6%B8%AC%E5%AE%9A%E8%A9%A6%E5%8A%91%E7%9B%92-Laccase-Activity-Assay-Kit-AK0319-1.png\" width=\"815\" height=\"601\" \/><\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>\u6ce8\u610f\u4e8b\u9805\uff1a<\/strong><\/span><\/p>\n<p>1. \u4f7f\u7528\u6642\u914d\u88fd\u5de5\u4f5c\u6db2\u3002\u4e26\u5118\u5feb\u4f7f\u7528\u5b83\u3002 4\u2103\u4fdd\u5b581\u9031\u3002\u5982\u679c\u8b8a\u8272\uff0c\u5247\u7121\u6cd5\u4f7f\u7528\u3002<br \/>\n2. \u6e2c\u5b9a\u524d\u9032\u884c\u9810\u5be6\u9a57\u3002\u5982\u679c\u5438\u5149\u5ea6\u503c\u8f03\u9ad8\uff0c\u8acb\u7528\u63d0\u53d6\u6db2\u7a00\u91cb\u6a23\u54c1\u9032\u884c\u9069\u7576\u7684\u91cd\u65b0\u6e2c\u5b9a\u3002\u4e26\u4e58\u4ee5\u8a08\u7b97\u516c\u5f0f\u4e2d\u7684\u7a00\u91cb\u6bd4\u4f8b\u3002<br \/>\n3. blank tube\u662f\u7528\u65bc\u6aa2\u6e2c\u5404\u8a66\u5291\u6210\u5206\u8cea\u91cf\u7684\u8a66\u7ba1\u3002\u6b63\u5e38\u689d\u4ef6\u4e0b\uff0cOD\u503c\u8b8a\u5316\u4e0d\u8d85\u904e0.05\u3002<br \/>\n1. Prepare the working solution when it will be used. And use it as soon as possible. Keep it at 4\u2103 for one week. If it changes color, it cannot be used. 2. Carry out pre experiment before determination. If the absorbance value is high, please dilute the sample with the extraction solution for appropriate re determination. And multiply the dilution ratio in the calculation formula. 3. The blank tube is a test tube for testing the quality of each reagent component. Under normal conditions, the OD value does not change more than 0.05.<\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/wp-content\/plugins\/download-monitor\/download.php?id=3831\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/04\/SUNLONG_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x300.jpg\" \/><\/p>\n<p style=\"font-weight: 400;\"><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/04\/SUNLONGBIOTECH.jpg\" alt=\"\" \/><\/p>\n<p lang=\"en-US\"><span style=\"color: #800000;\">Sunlong Biotech \u53f0\u7063\u4ee3\u7406\u5546-Sunlong Biotech\u00a0<\/span><\/p>\n<p>Sunlong Biotech \u4f4d\u65bc\u4e2d\u570b\u676d\u5dde\uff0c\u5c08\u696d\u5f9e\u4e8belisa\u8a66\u5291\u76d2\uff0c\u8a3a\u65b7\u8a66\u5291\u76d2\uff0c\u6297\u9ad4\uff0c\u6297\u539f\uff0c\u751f\u547d\u79d1\u5b78\u7814\u7a76\u7684\u98df\u54c1\u5b89\u5168\u5361\u3002 \u5168\u9762\u7684\u7522\u54c1\u7dda\u652f\u6301\u6240\u6709\u4e3b\u8981\u751f\u547d\u79d1\u5b78\u7814\u7a76\u9818\u57df\u7684\u61c9\u7528\uff0c\u5305\u62ec\u5206\u5b50\u751f\u7269\u5b78\uff0c\u514d\u75ab\u5b78\uff0c\u7d30\u80de\u751f\u7269\u5b78\uff0c\u86cb\u767d\u8cea\u7d44\u5b78\u548c\u57fa\u56e0\u7d44\u5b78\u7814\u7a76\u3002Sunlong \u64c1\u6709\u81ea\u5df1\u7684\u54c1\u724c\uff0c\u540c\u6642\u8207\u7f8e\u570bUSCNK\uff0cCusabio\uff0cEiaab\uff0cBluegene\uff0cBioss\u7b49\u570b\u5167\u77e5\u540d\u5ee0\u5546\u5408\u4f5c\uff0c\u5c07\u6240\u6709\u751f\u7269\u884c\u696d\u77e5\u540d\u54c1\u724c\u7522\u54c1\u878d\u5165\u4e2d\u570b\u3002Sunlong\u5df2\u7d93\u5c07\u7522\u54c1\u5ef6\u4f38\u81f3\u5168\u7403\u4e8c\u5341\u591a\u500b\u570b\u5bb6\uff0c\u4e26\u8207\u6d77\u5916\u4f01\u696d\u5efa\u7acb\u4e86\u696d\u52d9\u95dc\u4fc2\uff0c\u4e26\u8207\u5408\u4f5c\u5925\u4f34\u5efa\u7acb\u4e86\u4e92\u5229\u7684\u95dc\u4fc2\u3002 Sunglong Biotech\u5c07\u901a\u904e\u63d0\u4f9b\u5ee3\u6cdb\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u8a66\u5291\uff0c\u8a66\u5291\u76d2\u548c\u6280\u8853\u4f86\u63d0\u9ad8\u751f\u7522\u529b\u4e26\u70ba\u60a8\u63d0\u4f9b\u5e6b\u52a9\u3002<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6f06\u9176\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2| Laccase Activity Assay Kit-AK0319 Laccase Ac [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":41066,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5551],"tags":[6173],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45557"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45557"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45557\/revisions"}],"predecessor-version":[{"id":45559,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45557\/revisions\/45559"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/41066"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45557"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45557"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}