{"id":45436,"date":"2023-08-29T17:59:25","date_gmt":"2023-08-29T09:59:25","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45436"},"modified":"2023-10-22T12:21:07","modified_gmt":"2023-10-22T04:21:07","slug":"hydroxyproline%e7%be%a5%e8%84%af%e6%b0%a8%e9%85%b8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91-akrivisbio%e8%b2%a8%e8%99%9fma-0101","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45436","title":{"rendered":"Hydroxyproline\u7fa5\u812f\u6c28\u9178\u6aa2\u6e2c\u8a66\u5291\u00a0| AkrivisBio\u8ca8\u865fMA-0101\u00a0"},"content":{"rendered":"

Hydroxyproline\u7fa5\u812f\u6c28\u9178\u6aa2\u6e2c\u8a66\u5291\u00a0| AkrivisBio<\/strong>\u8ca8\u865fMA-0101\u00a0<\/strong><\/a><\/span><\/p>\n

To measure 0.1-2 \u03bcg of hydroxyproline in samples<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u7fa5\u812f\u6c28\u9178\uff08MW 131.13\uff09\u662f\u4e00\u7a2e\u975e\u5fc5\u9700\u6c28\u57fa\u9178\uff0c\u4e3b\u8981\u5b58\u5728\u65bc\u52d5\u7269\u7684\u81a0\u539f\u86cb\u767d(collagen )\u548c\u5f48\u6027\u86cb\u767d(elastin)\u4e2d\u3002\u5bcc\u542b\u7fa5\u812f\u6c28\u9178\u7684\u86cb\u767d\u8cea( Hydroxyproline-rich proteins)\u662f\u4e5f\u5b58\u5728\u65bc\u690d\u7269\u7d30\u80de\u58c1\u4e2d\uff0c\u7528\u4f5c\u805a\u91a3\u9644\u8457\u9ede\u3002\u86cb\u767d\u8cea\u4e2d\u7fa5\u812f\u6c28\u9178\u7684\u5176\u4ed6\u60c5\u6cc1\u662f\u5df2\u77e5\u7684\uff0c\u4f46\u76f8\u5c0d\u7f55\u898b\uff08HIF-1\u3001\u828b\u87ba\u6bd2\u7d20\u7b49\uff09\u3002\u7fa5\u812f\u6c28\u9178\u662f\u5728\u5408\u6210\u5f8c\u901a\u904e\u812f\u6c28\u9170\u7fa5\u5316\u9176\u5728\u6297\u58de\u8840\u9178\u4f9d\u8cf4\u6027\u904e\u7a0b\u4e2d\u5f62\u6210\u7684\u80bd\u93c8\u3002\u5b83\u901a\u904e\u589e\u5f37\u81a0\u539f\u86cb\u767d\u7684\u6a5f\u68b0\u5b8c\u6574\u6027\u4f86\u767c\u63ee\u4f5c\u7528\u3002\u75be\u75c5\u72c0\u614b\u4e0b\u7684\u81a0\u539f\u86cb\u767d\u5f62\u6210\u548c\u5468\u8f49\uff0c\u5982\u809d\u7e96\u7dad\u5316(liver fibrosis)\u3001\u4f69\u5409\u7279\u6c0f\u9aa8\u8f49\u79fb(Paget\u2019s, bone metastases)\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf\u6c34\u89e3\u7522\u7269\u6216\u8840\u6e05\u548c\u5c3f\u6db2\u7fa5\u812f\u6c28\u9178(hydroxyproline)\u6c34\u5e73\u4f86\u8a55\u4f30\u3002 AkrivisBio \u7684\u7fa5\u812f\u6c28\u9178\u6e2c\u5b9a\u4e3b\u8981\u7528\u65bc\u6e2c\u91cf\u86cb\u767d\u8cea\u548c\u7d44\u7e54\u6c34\u89e3\u7522\u7269\u4e2d\u7684\u7fa5\u812f\u6c28\u9178\u3002\u8840\u6e05\u548c\u5c3f\u6db2\u53ef\u7528\u4f5c\u7d93\u904e\u9069\u7576\u9810\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u3002 AkrivisBio \u63d0\u4f9b\u4e86\u4e00\u7a2e\u6e2c\u91cf\u7fa5\u812f\u6c28\u9178\u7684\u7c21\u55ae\u65b9\u6cd5\uff0c\u53ef\u7522\u751f\u8272\u539f\u6e2c\u91cf\u7bc4\u570d\u70ba 550-565 nm\uff0c\u9069\u7528\u7bc4\u570d\u70ba 1-20 \u03bcg \u81a0\u539f\u86cb\u767d\u6216\u7d04 0.1-2 \u03bcg \u7fa5\u812f\u6c28\u9178(hydroxyproline)\u3002<\/p>\n

Assay Principle:<\/strong><\/span>
\n1 – Samples are thoroughly hydrolyzed in acid
\n2 – Hydroxyproline is oxidized by Chloramine T to a pyrrole
\n3 \u2013 The pyrrole is subsequently reacted with Ehrlich\u2019s Reagent to form a chromophore<\/p>\n

\u6a23\u54c1\u88fd\u5099\uff1a<\/strong><\/span>
\n\u4f7f\u7528 100 \u03bcl H2O \/ 10 mg \u7d44\u7e54\u5747\u8cea\u5316\u6a23\u54c1\u3002\u5728\u53ef\u627f\u53d7\u58d3\u529b\u7684\u5bb9\u5668\u4e2d\u6dfb\u52a0\u7b49\u9ad4\u7a4d\u7684\u6fc3 HCl\uff08~12N\uff0c\u672a\u63d0\u4f9b\uff09\u5c0f\u74f6\uff08\u5982 Nalgene Micro Vial\uff09\uff0c\u5145\u5206\u6df7\u5408\u4f75\u5728 120\u00b0C \u4e0b\u6c34\u89e3 3 \u5c0f\u6642\u3002
\n\u7528\u7b49\u9ad4\u7a4d\u7684\u6fc3 HCl \u8655\u7406\u5c3f\u6db2\u6a23\u672c\u4e26\u4ee5\u540c\u6a23\u7684\u65b9\u5f0f\u9032\u884c\u6c34\u89e3\u3002\u7136\u5f8c\u61c9\u7528\u6d3b\u6027\u70ad\uff081 \u6beb\u514b\/50 \u5fae\u5347\u5c3f\u6db2\/HCl \u6df7\u5408\u7269\uff09\u8655\u7406\u5c3f\u6db2\u6a23\u672c\u3002\u96e2\u5fc3 3 \u5206\u9418\u3002\u53bb\u9664\u6d3b\u6027\u70ad\u3002\u5c07 10 \u03bcl \u6bcf\u7a2e\u6c34\u89e3\u6a23\u54c1\u8f49\u79fb\u81f3 96 \u5b54\u677f\u4e2d\uff0c\u4e26\u5728\u52a0\u71b1\u548c\/\u6216\u771f\u7a7a\u4e0b\u84b8\u767c\u81f3\u4e7e\u71e5\u3002\u6ce8\u610f\uff1a\u5167\u6e90\u6027\u5316\u5408\u7269\u53ef\u80fd\u6703\u5e72\u64fe\u53cd\u61c9\u3002\u78ba\u4fdd\u6e2c\u8a66\u4e2d\u7fa5\u812f\u6c28\u9178\u7684\u6e96\u78ba\u6e2c\u5b9a\u6a23\u54c1\u4e2d\uff0c\u6211\u5011\u5efa\u8b70\u5411\u6a23\u54c1\u4e2d\u6dfb\u52a0\u5df2\u77e5\u91cf\u7684\u6a19\u6e96\u54c1 (0.4 \u00b5g)\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nMA-0101<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 Reactions<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nHydroxyproline Assay kit
\n\u2022 Detection method- Absorbance (560 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- The assay is useful over the range of 0.1-2 \u03bcg.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (OD 550-565 nm).<\/td>\n<\/tr>\n
Species Reactivity
\nSample Type
\n<\/strong><\/td>\n		All
\nTissue hydrolysates, Serum, Urine<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nEstimation of hydroxyproline concentration in various biological samples<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\nOxidation Buffer 10 ml
\nChloramine T Concentrate 0.6 ml
\nAcid\/Isopropanol Solution 5 ml
\nDMAB Concentrate 5 ml
\nHydroxyproline Standard (1 mg\/ml) 0.1 ml
\nStorage Conditions\u00a0<\/strong>+4\u00b0C<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Note: Many metabolites and other chemicals can interfere significantly with a variety of reaction chemistries. For more precise
\ndeterminations, perform a standard curve in the absence and presence of a constant amount of sample. It\u2019s not necessary to
\nrun all six standards; use a minimum of 3 (0 \u2013 10 \u2013 20 \u00b5l) to verify that the OD response is linear with amount of analyte. The
\nslopes of the two std curves should be the same. The OD offset between them is attributable to the amount of analyte
\n(hydroxyproline in the current case) in the sample. If the two slopes are different, there is a matrix effect influencing the reaction
\nchemistry. In that case determine the OD difference between the 0 standards and apply the slope of the standard curve run in
\nthe presence of sample to it.<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Hydroxyproline\u7fa5\u812f\u6c28\u9178\u6aa2\u6e2c\u8a66\u5291\u00a0| AkrivisBio\u8ca8\u865fMA-0101\u00a0 To measur […]<\/p>\n","protected":false},"author":3,"featured_media":45835,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6087],"tags":[6184],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45436"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45436"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45436\/revisions"}],"predecessor-version":[{"id":45440,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45436\/revisions\/45440"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/45835"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45436"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45436"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45436"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}