{"id":45046,"date":"2023-08-13T15:46:31","date_gmt":"2023-08-13T07:46:31","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45046"},"modified":"2025-11-15T14:54:25","modified_gmt":"2025-11-15T06:54:25","slug":"%e5%87%8b%e4%ba%a1%e8%9b%8b%e7%99%bd%e9%85%b65-%e8%9e%a2%e5%85%89%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90-caspase-5-fluorometric-assay-kit-apexbio%e8%b2%a8%e8%99%9fk2195","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45046","title":{"rendered":"\u51cb\u4ea1\u86cb\u767d\u91765 \u87a2\u5149\u6d3b\u6027\u5206\u6790 | Caspase-5 Fluorometric Assay Kit APExBIO\u8ca8\u865fK2195"},"content":{"rendered":"

\u51cb\u4ea1\u86cb\u767d\u91765 \u87a2\u5149\u6d3b\u6027\u5206\u6790-Caspase-5 Fluorometric Assay Kit<\/span>
\n<\/span><\/span><\/span><\/strong><\/span><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291 (Small Molecule Inhibitors)\/\u6d3b\u5316\u5291(Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

 <\/p>\n

\u51cb\u4ea1\u86cb\u767d\u91765 \u87a2\u5149\u6d3b\u6027\u5206\u6790| Caspase-5 Fluorometric Assay Kit APExBIO\u8ca8\u865fK2195<\/span><\/a><\/span><\/strong><\/p>\n

Convenient, Highly-Sensitive Assay.<\/p>\n

Caspase-5\u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u51cb\u4ea1\u86cb\u767d\u91765\u87a2\u5149\u6d3b\u6027\u5206\u6790) WEHD-AFC<\/p>\n

Caspase-5 \u5c6c\u65bc\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176(cysteine proteases)\u7684 Caspase \u5bb6\u65cf\u3002 Caspase-5 \u662f\u4e00\u7a2e\u5728\u5929\u51ac\u6c28\u9178\u6b98\u57fa\u8655(aspartic acid residue)\u4ee5\u86cb\u767d\u6c34\u89e3\u65b9\u5f0f\u88c2\u89e3\u5176\u4ed6\u86cb\u767d\u8cea\u7684\u9176\u3002 Caspase-5 \u4f5c\u70ba\u7121\u6d3b\u6027\u7684\u9176\u539f\u5b58\u5728\u65bc\u7d30\u80de\u4e2d\uff0c\u4e26\u901a\u904e\u86cb\u767d\u6c34\u89e3\u800c\u6210\u719f\u3002\u6d3b\u6027 Caspase-5 \u662f\u5305\u542b\u5169\u500b\u5927\u4e9e\u57fa\u548c\u5169\u500b\u5c0f\u4e9e\u57fa\u7684\u7570\u56db\u805a\u9ad4\u3002 Caspase-5\u7684\u529f\u80fd\u88ab\u8a8d\u70ba\u662f\u4e00\u7a2e\u708e\u75c7\u6027Caspase\uff0c\u8207Caspase-1\u3001Caspase-4\u4e00\u8d77\u5728\u514d\u75ab\u7cfb\u7d71\u4e2d\u767c\u63ee\u4f5c\u7528\u3002\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u7d30\u80de\u51cb\u4ea1\u662f\u7531 ICE \u5bb6\u65cf\u86cb\u767d\u9176( ICE-family proteases)\/\u534a\u80f1\u5929\u51ac\u9176(caspases)\u7684\u6fc0\u6d3b\u5f15\u8d77\u7684\u3002 Caspase-5 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u3001\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c Caspase-5 \u548c\u5176\u4ed6\u76f8\u95dc\u7684 caspase \u7684\u6d3b\u6027\u3002\u9019\u4e9b\u534a\u80f1\u5929\u51ac\u9176\u53ef\u4ee5\u8b58\u5225\u5e8f\u5217 WEHD\u3002\u8a72\u6e2c\u5b9a\u662f\u6839\u64da\u5e95\u7269WEHD-AFC\uff08AFC\uff1a7-\u6c28\u57fa-4-\u4e09\u6c1f\u7532\u57fa\u9999\u8c46\u7d20\uff09\u88c2\u89e3\u7684\u6aa2\u6e2c\u3002 WEHD-AFC \u767c\u5c04\u85cd\u5149\uff08\u03bbmax = 400 nm\uff09\uff1b\u6e38\u96e2 AFC \u5728\u88ab Caspase-5 \u6216\u5176\u4ed6\u76f8\u95dc\u7684 caspase \u88c2\u89e3\u5e95\u7269\u5f8c\u767c\u51fa\u9ec3\u7da0\u8272\u87a2\u5149\uff08\u03bbmax = 505 nm\uff09\u3002\u4f7f\u7528\u7192\u5149\u8a08\u6216\u87a2\u5149\u5fae\u91cf\u6ef4\u5b9a\u677f\u8b80\u6578\u5668\u53ef\u4ee5\u91cf\u5316\u7192\u5149\u3002\u5c07\u8655\u7406\u904e\u7684\u6a23\u54c1\u8207\u672a\u8655\u7406\u7684\u5c0d\u7167\u7684 AFC \u87a2\u5149\u9032\u884c\u6bd4\u8f03\uff0c\u53ef\u4ee5\u78ba\u5b9a Caspase-5 \u6d3b\u6027\u7684\u500d\u6578\u589e\u52a0\u3002<\/p>\n

\n
\n

Background<\/span><\/h2>\n<\/div>\n
\n

Caspase-5 belongs to the Caspase-family of cysteine proteases. Caspase-5 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-5 exists in cells as an inactive proenzyme and is matured by proteolysis. The active Caspase-5 is a heterotetramer containing two large and two small subunits. The function of Caspase-5 is believed to be an inflammatory Caspase, together with Caspase-1, Caspase-4, and has a role in the immune system.<\/p>\n

Apoptosis in mammalian cells is caused by activation of ICE-family proteases\/caspases. The Caspase-5 Fluorometric Assay Kit provides a fast and convenient means to assay the activity of Caspase-5 and other related caspases. These caspases can recognize the sequence WEHD. The assay is according to the detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (\u03bbmax = 400 nm); free AFC emits a yellow-green fluorescence ( \u03bbmax = 505 nm) upon cleavage of the substrate by Caspase-5 or other related caspases. Using a fluorometer or fluorecence microtiter plate reader can quantify the fluorescence. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.<\/p>\n<\/div>\n

\n

Features & Properties<\/span><\/h2>\n<\/div>\n
\n\n\n\n\n\n
Features<\/td>\nFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.<\/td>\n<\/tr>\n
Shipping<\/td>\nGel pack.<\/td>\n<\/tr>\n
Storage Conditions<\/td>\nStore at -20\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n

https:\/\/www.apexbt.com<\/a><\/span><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u51cb\u4ea1\u86cb\u767d\u91765 \u87a2\u5149\u6d3b\u6027\u5206\u6790-Caspase-5 Fluorometric Assay Kit APExBIO […]<\/p>\n","protected":false},"author":3,"featured_media":45049,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[6033],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45046"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45046"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45046\/revisions"}],"predecessor-version":[{"id":53674,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45046\/revisions\/53674"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/45049"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45046"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45046"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45046"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}