{"id":45040,"date":"2023-08-13T14:59:53","date_gmt":"2023-08-13T06:59:53","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=45040"},"modified":"2025-11-15T15:00:34","modified_gmt":"2025-11-15T07:00:34","slug":"%e5%87%8b%e4%ba%a1%e8%9b%8b%e7%99%bd%e9%85%b61%e8%9e%a2%e5%85%89%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90-caspase-1-fluorometric-assay-kit-apexbio%e8%b2%a8%e8%99%9fk2010","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=45040","title":{"rendered":"\u51cb\u4ea1\u86cb\u767d\u91761\u87a2\u5149\u6d3b\u6027\u5206\u6790(YVAD-AFC) | Caspase-1 Fluorometric Assay Kit APExBIO\u8ca8\u865fK2010"},"content":{"rendered":"

\u51cb\u4ea1\u86cb\u767d\u91761 \u87a2\u5149\u6d3b\u6027\u5206\u6790-Caspase-1 Fluorometric Assay(YVAD-AFC)<\/span>
\n<\/span><\/span><\/span><\/strong><\/span><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291 (Small Molecule Inhibitors)\/\u6d3b\u5316\u5291(Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

\u51cb\u4ea1\u86cb\u767d\u91761 \u87a2\u5149\u6d3b\u6027\u5206\u6790| Caspase-1 Fluorometric Assay KitAPExBIO\u8ca8\u865fK2010<\/span><\/a><\/span><\/strong><\/p>\n

Caspase-1 Fluorometric Assay Kit Detects YVAD-dependent caspase activity, highly-sensitive<\/p>\n

Caspase-1 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u51cb\u4ea1\u86cb\u767d\u91761\u87a2\u5149\u6d3b\u6027\u5206\u6790) YVAD-AFC<\/p>\n

\u534a\u80f1\u6c28\u9178\u4f9d\u8cf4\u6027\u5929\u51ac\u6c28\u9178\u5b9a\u5411\u86cb\u767d\u9176\uff08Cysteine-dependent aspartate-directed proteases , Caspase\uff09\u662f\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176\u5bb6\u65cf(cysteine proteases)\uff0c\u5728\u7d30\u80de\u51cb\u4ea1(apoptosis)\u3001\u58de\u6b7b(necrosis)\u548c\u708e\u75c7( inflammation)\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002 Caspases \u7684\u9023\u7e8c\u6fc0\u6d3b\u5728\u7d30\u80de\u51cb\u4ea1(cell apoptosis)\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002 Caspase-1\uff0c\u4e5f\u7a31\u70ba IL-1\u03b2 \u8f49\u63db\u9176 (IL-1\u03b2-converting enzyme , ICE)\uff0c\u662f\u5148\u5929\u514d\u75ab\u7684\u95dc\u9375\u8abf\u7bc0\u56e0\u5b50\u3002\u6d3b\u6027 Caspase-1 \u88c2\u89e3\u4e26\u6fc0\u6d3b\u4fc3\u708e\u6027 IL-1\u03b2 \u548c IL-18\uff0c\u4e26\u4fc3\u9032\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u7d30\u80de\u7126\u4ea1(pyroptosis)\u8a98\u5c0e\u7684\u708e\u75c7\u5f62\u5f0f\u7684\u7d30\u80de\u6b7b\u4ea1\u3002Caspase-1 \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\u3001\u7c21\u55ae\u7684\u65b9\u6cd5\u4f86\u6aa2\u6e2c YVAD \u4f9d\u8cf4\u6027 caspase \u6d3b\u6027\u3002 YVAD-AFC\uff08AFC\uff1a7-\u6c28\u57fa-4-\u4e09\u6c1f\u7532\u57fa\u9999\u8c46\u7d20\uff09\u767c\u5c04\u85cd\u5149\uff08\u03bbmax = 400 nm\uff09\uff1b\u7576YVAD-AFC\u88abCaspase-1\u6216\u76f8\u95dc\u7684caspase\u88c2\u89e3\u6642\uff0c\u6e38\u96e2\u7684AFC\u6703\u767c\u51fa\u9ec3\u7da0\u8272\u87a2\u5149\uff08\u03bbmax = 505 nm\uff09\uff0c\u53ef\u4ee5\u4f7f\u7528\u9176\u6a19\u5100\u6216\u87a2\u5149\u8a08\u9032\u884c\u5b9a\u91cf\u3002\u5c07\u51cb\u4ea1\u6a23\u54c1\u7684 AFC \u87a2\u5149\u8207\u672a\u8a98\u5c0e\u7684\u5c0d\u7167\u9032\u884c\u6bd4\u8f03\uff0c\u53ef\u78ba\u5b9a Caspase-1 \u6d3b\u6027\u7684\u500d\u6578\u589e\u52a0\u3002<\/p>\n\n\n\n\n\n\n\n\n
Components<\/b><\/td>\n\n

K2010-25<\/p>\n

25 Assays<\/td>\n

\n

K2010-100<\/p>\n

100 Assays<\/td>\n

\n

K2010-200<\/p>\n

200 Assays<\/td>\n

\n

K2010-400<\/p>\n

400 Assays<\/td>\n<\/tr>\n

Cell Lysis Buffer<\/b><\/td>\n25 mL<\/td>\n100 mL<\/td>\n100 mL<\/td>\n100 mL<\/td>\n<\/tr>\n
2X Reaction Buffer<\/b><\/td>\n2 mL<\/td>\n4X 2 mL<\/td>\n16 mL<\/td>\n32 mL<\/td>\n<\/tr>\n
YVAD-AFC (1 mM)<\/b><\/td>\n125 \u00b5L<\/td>\n500 \u00b5L<\/td>\n2X 0.5 mL<\/td>\n2X 1 mL<\/td>\n<\/tr>\n
DTT (1 M)<\/b><\/td>\n100 \u00b5L<\/td>\n400 \u00b5L<\/td>\n400 \u00b5L<\/td>\n400 \u00b5L<\/td>\n<\/tr>\n
Store the components at -20\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

<\/p>\n

\n

Description<\/span><\/h2>\n<\/div>\n
\n

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-1, also known as IL-1\u03b2-converting enzyme (ICE), is a key regulator of innate immunity. The active Caspase-1 cleaves and activates pro-inflammatory IL-1\u03b2 and IL-18, and promotes an inflammatory form of cell death induced by apoptosis and pyroptosis.<\/p>\n

Caspase-1 Fluorometric Assay Kit provides a convenient and simple way for detecting the YVAD-dependent caspase activity. YVAD-AFC (AFC:7-amino-4-trifluoromethyl coumarin) emits blue light (\u03bbmax = 400 nm); while cleavage of YVAD-AFC by Caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (\u03bbmax = 505 nm), which can be quantified by using a microplate reader or a fluorometer. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-1 activity.<\/p>\n<\/div>\n

\n

Features<\/span><\/h2>\n<\/div>\n
\n

Simple one-step procedure; takes 1-2 hours<\/p>\n

Fast and convenient<\/p>\n

Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.<\/p>\n<\/div>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

<\/p>\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n

https:\/\/www.apexbt.com<\/a><\/span><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u51cb\u4ea1\u86cb\u767d\u91761 \u87a2\u5149\u6d3b\u6027\u5206\u6790-Caspase-1 Fluorometric Assay(YVAD-AFC) AP […]<\/p>\n","protected":false},"author":3,"featured_media":45049,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[6036],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45040"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45040"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45040\/revisions"}],"predecessor-version":[{"id":53681,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/45040\/revisions\/53681"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/45049"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45040"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45040"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45040"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}