{"id":44875,"date":"2023-08-07T15:40:17","date_gmt":"2023-08-07T07:40:17","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44875"},"modified":"2023-10-22T12:22:22","modified_gmt":"2023-10-22T04:22:22","slug":"resazurin-cell-viability-assay-kit-alamarblue-biotium-%e8%b2%a8%e8%99%9f30025-1-25ml","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=44875","title":{"rendered":"Resazurin Cell Viability Assay Kit (alamarBlue\u2122) | Biotium \u8ca8\u865f#30025-1 (25ml)\u00a0"},"content":{"rendered":"

Resazurin Cell Viability Assay Kit (alamarBlue\u2122) | Biotium \u8ca8\u865f#30025-1<\/a> (25ml)\u00a0<\/span><\/strong><\/p>\n

A sensitive, safe and cost-effective high-throughput homogeneous assay of cell viability by fluorescence or absorbance measurement.<\/span><\/p>\n

\u5203\u5929\u9752\u7d30\u80de\u6d3b\u529b\u6e2c\u5b9a(Resazurin Cell Viability Assay)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u5feb\u901f\u3001\u53ef\u9760\u3001\u9748\u654f\u3001\u5b89\u5168\u4e14\u7d93\u6fdf\u9ad8\u6548\u7684\u7d30\u80de\u6d3b\u529b\u6e2c\u91cf\u65b9\u6cd5\u3002\u6b64\u9069\u7528\u87a2\u5149\u548c\u53ef\u898b\u5149\u5075\u6e2c\u3002\u8207\u5176\u4ed6\u57fa\u65bc\u5203\u5929\u9752(resazurin-based )\u7684\u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2\uff08\u4f8b\u5982 alamarBlue\u00ae\u3001PrestoBlue\u00ae \u6216 CellTiter-Blue\u00ae\uff09\u76f8\u6bd4\uff0c\u8a72\u6e2c\u5b9a\u5177\u6709\u512a\u7570\u7684\u6027\u80fd\u3002\u55ae\u6b65\u3001\u540c\u8cea\u3001\u9ad8\u901a\u91cf\u7d30\u80de\u5b9a\u91cf\u7528\u65bc\u57fa\u65bc\u87a2\u5149\u6216\u5438\u5149\u5ea6\u7684\u6e2c\u91cf\u9748\u654f\uff0c\u53ea\u9700 80 \u500b\u7d30\u80de\u5373\u53ef\u7372\u5f97\u53ef\u91cd\u8907\u7684\u7d50\u679c\u3002\u5ee3\u6cdb\u7528\u65bc\u57f9\u990a\u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de(cultured mammalian cells)\u3001\u7d30\u83cc(bacteria)\u3001\u539f\u751f\u52d5\u7269(protozoa)\u3001\u9175\u6bcd(yeast)\u548c\u771f\u83cc(fungi)\uff1b<\/span>\u4e0d\u9700\u8981\u7d30\u80de\u88c2\u89e3(Cell lysis )\uff0c\u5141\u8a31\u9577\u671f\u52d5\u529b\u5b78\u76e3\u6e2c\u7121\u6bd2\u3001\u7121\u653e\u5c04\u6027\u3001\u7121\u81f4\u764c\u6027\u3001\u8655\u7f6e\u7c21\u55ae\u5373\u7528\u578b\u89e3\u6c7a\u65b9\u6848\u3002\u5203\u5929\u9752(Resazurin)\u901a\u904e\u97ff\u61c9\u7d30\u80de\u751f\u9577\u5f15\u8d77\u7684\u751f\u9577\u57f9\u990a\u57fa\u7684\u5316\u5b78\u9084\u539f\uff0c\u5f9e\u975e\u87a2\u5149\u67d3\u6599\u8f49\u8b8a\u70ba\u9ad8\u7d05\u8272\u87a2\u5149\u67d3\u6599\u8a66\u9e75\u9748\u4f86\u6aa2\u6e2c\u7d30\u80de\u4ee3\u8b1d\u3002\u6301\u7e8c\u7684\u7d30\u80de\u751f\u9577\u7dad\u6301\u9084\u539f\u74b0\u5883\uff0c\u800c\u6291\u5236\u751f\u9577\u5247\u7dad\u6301\u6c27\u5316\u74b0\u5883\u3002\u5203\u5929\u9752(Resazurin)\u539f\u70ba\u6df1\u85cd\u8272<\/strong>\u3001\u7121\u87a2\u5149\u6027\uff0c\u7136\u800c\u7d93\u904e\u7c92\u7dda\u9ad4\u4e2d\u7684NADH \u53bb\u6c2b\u9176\u4f5c\u7528\u5f8c\uff0c\u5203\u5929\u9752(Resazurin)\u9084\u539f\u6210\u70ba\u7c89\u7d05\u8272<\/strong>\uff0c<\/strong>\u900f\u904e\u5075\u6e2c\u87a2\u5149\u00a0<\/strong>(Ex\/Em: 530-560 nm\/590 nm) \u6216\u5438\u5149\u503c (570 nm) \u5438\u5149\u5ea6\u9032\u884c\u6aa2\u6e2c\u3002\u87a2\u5149\u6216\u6bd4\u8272\u4fe1\u865f\u8207\u6a23\u54c1\u4e2d\u6d3b\u7d30\u80de\u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002\u6dfb\u52a0\u5e95\u7269\u5f8c 1-24 \u5c0f\u6642\u5373\u53ef\u7372\u5f97\u7d50\u679c\u3002\u5203\u5929\u9752\u5373\u7528\u578b\u6eb6\u6db2(Resazurin solution)\u4ee5 25 mL\uff082500 \u6b21\u6e2c\u5b9a\uff09\u6216 100 mL\uff0810,000 \u6b21\u6e2c\u5b9a\uff09\u898f\u683c\u63d0\u4f9b\u3002<\/p>\n

\u5203\u5929\u9752(Resazurin) Cell Viability Assay\u512a\u9ede<\/strong><\/span><\/p>\n

\u5ee3\u6cdb\u7528\u65bc\u57f9\u990a\u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de(cultured mammalian cells)\u3001\u7d30\u83cc(bacteria)\u3001\u539f\u751f\u52d5\u7269(protozoa)\u3001\u9175\u6bcd(yeast)\u548c\u771f\u83cc(fungi)<\/p>\n

1. \u5141\u8a31\u9577\u671f\u52d5\u529b\u5b78\u76e3\u6e2c (\u7121\u6bd2\u3001\u7121\u653e\u5c04\u6027\u3001\u7121\u81f4\u764c\u6027\u3001\u7121\u7d30\u80de\u6eb6\u89e3) \u76f4\u63a5\u52a0\u5165\u57f9\u990a\u6db2\u4e2d\uff0c\u540c\u6b65\u53ca\u6642\u9032\u884c\u85e5\u7269\u5c0d\u7d30\u80de\u6bd2\u6027\u7684\u52d5\u529b\u5b78\u5206\u6790
\n2.\u64cd\u4f5c\u7c21\u55ae\uff0c\u76f4\u63a5\u57f9\u990a\u65bcmedium\u4e2d30\u5206\u9418\u53cd\u61c9
\n3. \u4e0d\u9808DMSO\u56de\u6eb6\u6b65\u9a5f\uff0c\u6545\u4e0d\u6613pipetting error\uff0c\u8f03MTT assay\u6e96\u78ba
\n4. \u9748\u654f\u5ea6\u8f03\u4f73\uff0c 80 \u500b\u7d30\u80de\u5373\u53ef\u7372\u5f97\u53ef\u91cd\u8907\u7684\u7d50\u679c
\n5.\u9069\u7528\u87a2\u5149\u6216\u662f\u53ef\u898b\u5149\u6a5f\u5668<\/p>\n

\u67e5\u770b\u6211\u5011\u5b8c\u6574\u7684\u7d30\u80de\u6d3b\u529b\u548c\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u9078\u64c7 (Cell Viability & Apoptosis Assays<\/a>)<\/p>\n

\"BIOTIUM_\u5de5\u4f5c\u5340\u57df<\/p>\n

Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This assay has excellent performance compared to other resazurin-based cell proliferation kits such as alamarBlue\u00ae, PrestoBlue\u00ae, or CellTiter-Blue\u00ae.<\/p>\n

\u7d30\u80de\u751f\u9577\u5275\u9020\u4e86\u4e00\u500b\u6e1b\u5c11\u7684\u74b0\u5883\uff0c\u540c\u6642\u6291\u5236\u751f\u9577\u6c27\u5316\u74b0\u5883\u3002\u97ff\u61c9\u751f\u9577\u57f9\u990a\u57fa\u7684\u5316\u5b78\u9084\u539f\u5203\u5929\u9752\uff08\u7d2b\u8272\u4e14\u7121\u87a2\u5149\uff09\u662f\u7d30\u80de\u751f\u9577\u7684\u7d50\u679c\u9084\u539f\u5f62\u6210\u7d05\u8272\u87a2\u5149\u67d3\u6599\u8a66\u9e75\u9748 (1-3)\u3002\u5203\u5929\u9752\u7684\u9084\u539f\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf\u87a2\u5149\u6216\u5438\u5149\u5ea6\u4f86\u76e3\u6e2c\u3002\u87a2\u5149\u548c\u6e2c\u5b9a\u7522\u751f\u7684\u6bd4\u8272\u4fe1\u865f\u8207\u6d3b\u9ad4\u6578\u91cf\u6210\u6b63\u6bd4\u6a23\u54c1\u4e2d\u7684\u7d30\u80de\u3002\u5203\u5929\u9752\u6e2c\u5b9a\u7684\u9748\u654f\u5ea6\u8207 [3H]\u80f8\u82f7\u6e2c\u5b9a\u6aa2\u6e2c\u7d30\u80de\u589e\u6b96\uff081\uff09\u3002\u6839\u64da\u7d30\u80de\u985e\u578b\uff0c\u5203\u5929\u9752\u6e2c\u5b9a\u53ef\u4ee5\u53ef\u7528\u65bc\u6aa2\u6e2c\u5c11\u81f3 40 \u500b\u7d30\u80de\uff0c\u4e26\u5177\u6709\u53ef\u91cd\u8907\u4e14\u9748\u654f\u7684\u4fe1\u865f\u3002<\/p>\n

\n

alamarBlue \u662f Trek Biosystems \u7684\u8a3b\u518a\u5546\u6a19\u3002 CellTiter-Blue \u662f Promega \u516c\u53f8\u7684\u8a3b\u518a\u5546\u6a19\u3002 PrestoBlue \u662f Thermo Fisher Scientific \u7684\u8a3b\u518a\u5546\u6a19\u3002alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation. PrestoBlue is a registered trademark of Thermo Fisher Scientific.<\/p>\n\n\n\n\n\n\n\n\n\n
Apoptosis\/viability marker<\/th>\nMetabolic activity<\/td>\n<\/tr>\n
For live or fixed cells<\/th>\nFor live\/intact cells<\/td>\n<\/tr>\n
Detection method\/readout<\/th>\nMicroplate reader (absorbance), Microplate reader (fluorescence)<\/td>\n<\/tr>\n
Assay type\/options<\/th>\nEndpoint assay, High-throughput assay, Homogeneous assay, Real-time imaging<\/td>\n<\/tr>\n
Colors<\/th>\nRed<\/td>\n<\/tr>\n
Storage Conditions<\/th>\nStore at 2 to 8 \u00b0C<\/td>\n<\/tr>\n
Excitation\/Emission<\/th>\n530-560\/590 nm, 570 nm (for absorbance-based assay)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n<\/div>\n

\n

REFERENCES<\/span><\/h2>\n<\/div>\n
\n

1. Arterioscler, Thromb, Vasc Biol (2010) 30(10), 1940\u20131948.\u00a0DOI:10.1161\/ATVBAHA.110.205997<\/a>
\n2. J Clin Endocrinol Metab, October 2010, 95(10):E181\u2013E191\u00a0doi: 10.1210\/jc.2010-0581<\/a>
\n3. Nucleic Acids Res (2010) 38(22), 8131\u20138140.\u00a0doi:10.1093\/nar\/gkq697<\/a>
\n4. Scand J Clin Lab Invest. (2010) 70(7), 512-8.\u00a0doi: 10.3109\/00365513.2010.521255<\/a>
\n5. Tissue Eng A (2011) 17(1-2), 37-44.\u00a0http:\/\/doi.org\/10.1089\/ten.tea.2010.0188<\/a>
\n6. Biomaterials (2011) 32(1), 128-36.\u00a0doi: 10.1016\/j.biomaterials.2010.09.006.<\/a>
\n7. Food Chem (2012) 135(3), 1533-1538.\u00a0https:\/\/doi.org\/10.1016\/j.foodchem.2012.06.058<\/a>
\n8. Drug Des Dev Therapy (2013) 7, 529\u2013544.\u00a0http:\/\/dx.doi.org\/10.2147\/DDDT.S45162<\/a>
\n9. Langmuir (2013) 29(46), 14254-64.\u00a0doi: 10.1021\/la403533r<\/a>
\n10. PLoS ONE (2013) 8(10), e76264.\u00a0doi:10.1371\/journal.pone.0076264<\/a>
\n11. ACS Nano (2015) 9(2), 1219-1235.\u00a0https:\/\/doi.org\/10.1021\/nn504890z<\/a>
\n12. Antimicrob Agents Chemother (2016) 60(7), 4183\u20134196.\u00a0doi:10.1128\/AAC.03021-15<\/a>
\n13. Curr Prot Toxicol (2016) 68, 2.24.1-2.24.15.\u00a0doi: 10.1002\/cptx.1<\/a>
\n14. J Biol Chem (2016) 291, 21869-21879.\u00a0doi: 10.1074\/jbc.M115.712166<\/a>
\n15. Curr Prot Stem Cell Biol (2018) 26(1), 5C.3.1-5C.3.19.\u00a0DOI:10.1002\/9780470151808.sc05c03s26<\/a>
\n16. Int J Biol Macromol (2018) 113(1), 132-141.\u00a0https:\/\/doi.org\/10.1016\/j.ijbiomac.2018.02.069<\/a><\/p>\n<\/div>\n

\"\u54c1\u724c\u4ecb\u7d39_\u5de5\u4f5c\u5340\u57df<\/p>\n

\"biotium\"<\/p>\n

Biotium \u63d0\u4f9b\u7bc4\u570d\u5ee3\u6cdb\u7684\u87a2\u5149\u63a2\u91dd\u7528\u65bc\u7814\u7a76\u51cb\u4ea1\u7d30\u80de(Apotosis)<\/span><\/strong><\/p>\n

\"\"<\/p>\n

The\u00a0NucView\u00ae Caspase-3 Substrate<\/a>\u00a0comes in 3 colors, and measures caspase activity in real time, in live cells.\u00a0MitoView\u2122 633<\/a>\u00a0can measure the decrease in mitochondria membrane potential that occurs in early apoptosis.\u00a0CF\u00ae Dye Annexin V<\/a>\u00a0conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.<\/p>\n","protected":false},"excerpt":{"rendered":"

Resazurin Cell Viability Assay Kit (alamarBlue\u2122) | Biot […]<\/p>\n","protected":false},"author":3,"featured_media":44877,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5378],"tags":[6188],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44875"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44875"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44875\/revisions"}],"predecessor-version":[{"id":44888,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44875\/revisions\/44888"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/44877"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44875"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44875"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44875"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}