{"id":44571,"date":"2023-07-28T18:17:54","date_gmt":"2023-07-28T10:17:54","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44571"},"modified":"2024-05-20T11:38:00","modified_gmt":"2024-05-20T03:38:00","slug":"%e7%a1%9d%e9%85%b8%e9%b9%bd-%e4%ba%9e%e7%a1%9d%e9%85%b8%e9%b9%bd%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-nitrate-nitrite-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fabnova","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=44571","title":{"rendered":"\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| Nitrate\/Nitrite Colorimetric Assay Kit \u8ca8\u865fAbnova\u2122 KA1342"},"content":{"rendered":"

\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| Nitrate\/Nitrite Colorimetric Assay Kit \u8ca8\u865fAbnova\u2122 KA1342<\/span><\/a><\/strong><\/span><\/p>\n

Nitrate\/Nitrite Colorimetric Assay Kit, 96 assays<\/p>\n

<\/p>\n

\u4e00\u6c27\u5316\u6c2e (NO) \u5728\u751f\u7269\u7cfb\u7d71\u4e2d\u7531\u4e00\u6c27\u5316\u6c2e\u5408\u9176 (NOS) \u5408\u6210\u3002\u4e00\u6c27\u5316\u6c2e\u5408\u9176\u662f\u4e00\u500b\u975e\u5e38\u8907\u96dc\u7684\u9176\uff0c\u4f5c\u7528\u65bc\u5206\u5b50\u6c27\u3001\u7cbe\u6c28\u9178\u548c NADPH\uff0c\u7522\u751f NO\u3001\u74dc\u6c28\u9178\u3001\u548c NADP+\u3002\u9019\u500b\u904e\u7a0b\u9700\u8981\u4e94\u500b\u984d\u5916\u7684\u8f14\u52a9\u56e0\u5b50\uff08FMN\u3001FAD\u3001\u8840\u7d05\u7d20\u3001\u9223\u8abf\u86cb\u767d\u548c\u56db\u6c2b\u751f\u7269\u8776\u5464\uff09\u548c\u5169\u7a2e\u4e8c\u50f9\u967d\u96e2\u5b50\uff08\u9223\u548c\u8840\u7d05\u7d20\u9435\uff1b\u898b\u5716 1\uff09\u3002\u4e09\u7a2e\u4e0d\u540c\u7684\u4e9e\u578bNOS \u5df2\u88ab\u8b58\u5225\uff0c\u5982\u5716 2 \u6240\u793a\u3002<\/p>\n

<\/p>\n

<\/p>\n

NO \u7531\u795e\u7d93\u5143\u3001\u5167\u76ae\u7d30\u80de\u3001\u8840\u5c0f\u677f\u548c\u4e2d\u6027\u7c92\u7d30\u80de\u97ff\u61c9\u65bc\u7a69\u614b\u523a\u6fc0\u30021,2 \u9019\u7a2e NO \u88ab\u5feb\u901f\u6e05\u9664\uff08t1\/2 = 4 \u79d2\uff09\u4e26\u4ee5\u65c1\u5206\u6ccc\u65b9\u5f0f\u8d77\u4f5c\u7528\u8f49\u5c0e\u7d30\u80de\u4fe1\u865f\u3002 NO \u8207\u9ce5\u82f7\u9178\u74b0\u5316\u9176\u7684\u8840\u7d05\u7d20\u8f14\u57fa\u76f8\u4e92\u4f5c\u7528\uff0c\u6fc0\u6d3b
\n\u9176\u4e26\u5c0e\u81f4 cGMP \u6c34\u5e73\u5347\u9ad8\u3002 NO \u4e5f\u7531\u5176\u4ed6\u7d30\u80de\uff08\u5de8\u566c\u7d30\u80de\u3001\u6210\u7e96\u7dad\u7d30\u80de\u3001\u809d\u7d30\u80de\uff09\u4ee5\u5fae\u6469\u723e\u6fc3\u5ea6\u97ff\u61c9\u708e\u75c7\u6216\u6709\u7d72\u5206\u88c2\u523a\u6fc0\u3002\u5728\u9019\u7a2e\u60c5\u6cc1\u4e0b\uff0c\u751f\u7269\u5b78\u4f5c\u7528\u662f\u901a\u904e\u6c27\u5316\u6bd2\u6027\u9632\u79a6\u975e\u81ea\u8eab\u75c5\u539f\u9ad4\u3002\u9019\u4e9b\u975e\u5e38\u9ad8\u7684\u4e00\u6c27\u5316\u6c2e\u6c34\u5e73\u5c0e\u81f4\u904e\u6c27\u4e9e\u785d\u9178\u9e7d\u7684\u5f62\u6210\u3001\u9435\u786b\u7c07\u7684\u7834\u58de\u3001\u786b\u9187\u4e9e\u785d\u5316\u548c\u86cb\u767d\u8cea\u7684\u785d\u5316\u916a\u6c28\u9178\u6b98\u57fa\u3002\u56e0\u6b64\uff0c\u4e0d\u540c\u751f\u7269\u7cfb\u7d71\u4e2d\u7522\u751f\u7684 NO \u91cf\u53ef\u80fd\u6709\u5e7e\u500b\u6578\u91cf\u7d1a\u7684\u5dee\u7570\u5176\u7a0b\u5ea6\u53ca\u5176\u96a8\u5f8c\u7684\u5316\u5b78\u53cd\u61c9\u6027\u662f\u591a\u7a2e\u591a\u6a23\u7684\u3002NO\u5728\u9ad4\u5167\u7684\u6700\u7d42\u7522\u7269\u662f\u4e9e\u785d\u9178\u9e7d\uff08NO2-\uff09\u548c\u785d\u9178\u9e7d\uff08NO3-\uff09\u3002 NO2\u76f8\u5c0d\u6bd4\u4f8b- \u548cNO3-\u662f\u662f\u53ef\u8b8a\u7684\uff0c\u7121\u6cd5\u78ba\u5b9a\u5730\u9810\u6e2c\u3002\u56e0\u6b64\uff0c\u7e3d NO \u7522\u751f\u91cf\u7684\u6700\u4f73\u6307\u6a19\u662f\u5169\u8005\u4e4b\u548c (\u4e8c\u6c27\u5316\u6c2e- \u548cNO3)<\/p>\n

<\/p>\n

<\/pre>\n
\u6e2c\u5b9a\u539f\u7406 (Principle of the Assay)<\/span><\/strong><\/span><\/p>\n
\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(Nitrate\/Nitrite Colorimetric Assay Kit )\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6e96\u78ba\u3001\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u91cf\u7e3d\u785d\u9178\u9e7d( total nitrate\/nitrite)\u901a\u904e\u7c21\u55ae\u7684\u5169\u6b65\u904e\u7a0b\u5373\u53ef\u6e2c\u91cf\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6fc3\u5ea6(nitrate\/nitrite concentration)\u3002\u7b2c\u4e00\u6b65\u662f\u5229\u7528\u785d\u9178\u9084\u539f\u9176\u5c07\u785d\u9178\u9e7d(nitrate)\u8f49\u5316\u70ba\u4e9e\u785d\u9178\u9e7d(nitrite)\u3002\u7b2c\u4e8c\u6b65\u662f\u6dfb\u52a0 Griess \u8a66\u5291\uff0c\u5c07\u4e9e\u785d\u9178\u9e7d(nitrite)\u8f49\u5316\u70ba\u6df1\u7d2b\u8272\u5076\u6c2e\u5316\u5408\u7269\uff08\u898b\u5716 3\uff09\u3002\u8a72\u5076\u6c2e\u5316\u5408\u7269\u5438\u5149\u5ea6\u7684\u5149\u5ea6\u6e2c\u91cf\u9aee\u8272\u5718\u6e96\u78ba\u6e2c\u5b9a NO2- \u5c08\u6ce8\u3002NADPH \u662f NOS \u9176\u529f\u80fd\u7684\u91cd\u8981\u8f14\u52a9\u56e0\u5b50\u3002\u4e0d\u5e78\u7684\u662f\uff0cNADPH \u6703\u5e72\u64feGriess \u8a66\u5291\u7684\u5316\u5b78\u539f\u7406\uff0cGriess \u8a66\u5291\u662f\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u6700\u5e38\u7528\u7684\u8a66\u5291\u3002\u4e00\u7a2e\u65b9\u6cd5\u662f\u9632\u6b62\u9019\u7a2e\u5e72\u64fe\u7684\u65b9\u6cd5\u662f\u4f7f\u7528\u5c11\u91cf\u7684 NADPH \u8207\u50ac\u5316\u7cfb\u7d71\u7d50\u5408\u9032\u884c\u56de\u6536\u5c07 NADP+ \u8f49\u5316\u70ba NADPH\u3002\u9019\u662f\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(Nitrate\/Nitrite Colorimetric Assay Kit )\u4e2d\u4f7f\u7528\u7684\u7cfb\u7d71\u3002\u6548\u679c\u5f88\u597d\u7528\u65bc\u5206\u6790\u8840\u6f3f\u548c\u5c3f\u6db2\u7b49\u6db2\u9ad4\u4e2d\u7684\u785d\u9178\u9e7d(nitrate)\u548c\u4e9e\u785d\u9178\u9e7d(nitrite)\uff0c\u4e5f\u53ef\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u7684\u7528\u65bc\u6aa2\u6e2c\u4f4e\u6c34\u5e73\u4e9e\u785d\u9178\u9e7d(nitrite)\u7684\u87a2\u5149\u7248\u672c\u3002\u4f46\u5b83\u4e0d\u80fd\u7528\u65bc\u5206\u6790\u785d\u9178\u9e7d(nitrate)\u548c\u9ad4\u5916 NOS \u6e2c\u5b9a\u4e2d\u6dfb\u52a0\u4e86\u904e\u91cf NADPH \u7684\u4e9e\u785d\u9178\u9e7d(nitrite)\u3002\u5c0d\u65bc\u9019\u4e9b\u6e2c\u5b9a\uff0c\u9700\u8981\u9032\u884c\u7b2c\u4e8c\u6b21\u65b9\u6cd5\uff08LDH \u65b9\u6cd5\uff09\u7528\u65bc 96 \u5b54\u677f\u5f62\u5f0f\u3002<\/p>\n
<\/p>\n
\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\n<\/p>\n
<\/p>\n
<\/p>\n
Figure 6. Spike and recovery of Nitrate in human plasma (EDTA), saliva, and urine. Human plasma, urine,
\nand saliva were spiked with different amounts of nitrate, processed as described in the Sample Preparation
\nsection, serially diluted with Nitrate\/Nitrite Assay Buffer (1X), and evaluated using the Nitrate\/ Nitrite
\nColorimetric Assay Kit. The error bars represent standard deviations obtained from multiple dilutions of each
\nsample.<\/p>\n
\n
Publication Reference<\/b><\/span><\/p>\n
1.Zoonotic Visceral Leishmaniasis: New Insights on Innate Immune Response by Blood Macrophages and Liver Kupffer Cells to Leishmania infantum Parasites.<\/a>
\nArmanda Viana Rodrigues, Ana Val\u00e9rio-Bolas, Gra\u00e7a Alexandre-Pires, Maria Aires Pereira, Telmo Nunes, D\u00e1rio Ligeiro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes.Biology (Basel). 2022 Jan 9;11(1):100. doi: 10.3390\/biology11010100.<\/p>\n
2.Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages.<\/a>
\nOph\u00e9lie Rutschmann, Chiara Toniolo, John D McKinney.mBio. 2022 Sep 19;e0225122. doi: 10.1128\/mbio.02251-22. Online ahead of print.<\/p>\n
3.Morus macroura Miq. Fruit extract protects against acetic acid-induced ulcerative colitis in rats: Novel mechanistic insights on its impact on miRNA-223 and on the TNF\u03b1\/NF\u03baB\/NLRP3 inflammatory axis.<\/a>
\nRania M Salama, Samar F Darwish, Ismail El Shaffei, Noura F Elmongy, Nouran M Fahmy, Manal S Afifi, Ghada A Abdel-Latif.Food Chem Toxicol. 2022 May 18;165:113146. doi: 10.1016\/j.fct.2022.113146. Online ahead of print.<\/p>\n
4.Canine neutrophils cooperate with macrophages in the early stages of Leishmania infantum in vitro infection.<\/a>
\nPereira MA, Alexandre-Pires G, C?mara M, Santos M, Martins C, Rodrigues A, Adriana J, Passero LFD, da Fonseca IP, Santos-Gomes G.Parasite Immunol. 2019 Feb 8:e12617. doi: 10.1111\/pim.12617. [Epub ahead of print]<\/p>\n
5.Beetroot supplementation improves the physiological responses to incline walking.<\/a>
\nWaldron M, Waldron L, Lawlor C, Gray A, Highton J.Eur J Appl Physiol. 2018 Mar 15. [Epub ahead of print]<\/p>\n
6.Synthetic 3′,4′-Dihydroxyflavone Exerts Anti-Neuroinflammatory Effects in BV2 Microglia and a Mouse Model.<\/a>
\nKim N, Yoo HS, Ju YJ, Oh MS, Lee KT, Inn KS, Kim NJ, Lee JK.Biomol Ther (Seoul). 2018 Mar 1;26(2):210-217. doi: 10.4062\/biomolther.2018.008.<\/p>\n
7.Anti-Inflammatory Potential of Gandarusa (Gendarussa vulgaris Nees) and Soursoup (Annona muricata L) Extracts in LPS Stimulated-Macrophage Cell (RAW264.7).<\/a>
\nLaksmitawati DR, Prasanti AP, Larasinta N, Syauta GA, Hilda R, Ramadaniati HU, Widyastuti A, Karami N, Afni M, Rihibiha DD, Kusuma HSW, Widowati W.Journal of Natural Remedies. 2016 Apr;16(2)<\/p>\n
 <\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
\u785d\u9178\u9e7d\/\u4e9e\u785d\u9178\u9e7d\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2| Nitrate\/Nitrite Colorimetric Assay Kit […]<\/p>\n","protected":false},"author":3,"featured_media":40950,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5549],"tags":[6084],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44571"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44571"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44571\/revisions"}],"predecessor-version":[{"id":49455,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44571\/revisions\/49455"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40950"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44571"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44571"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44571"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}