{"id":44015,"date":"2023-07-13T18:51:50","date_gmt":"2023-07-13T10:51:50","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=44015"},"modified":"2023-07-18T11:09:27","modified_gmt":"2023-07-18T03:09:27","slug":"t4-dna-ligase-%e8%b2%a8%e8%99%9fg467-200-u-200-%ce%bcl","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=44015","title":{"rendered":"T4 DNA Ligase | \u8ca8\u865fG467 200 U (200 \u03bcl)"},"content":{"rendered":"

T4 DNA Ligase | \u8ca8\u865fG467 200 U (200 \u03bcl)<\/span><\/a><\/span><\/h1>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nG467<\/span><\/td>\n<\/tr>\n
Name<\/td>\nT4 DNA Ligase<\/td>\n<\/tr>\n
Unit<\/td>\n200 U (200 \u03bcl)<\/td>\n<\/tr>\n
Category<\/td>\nMolecular Biology Enzymes and Kits<\/td>\n<\/tr>\n
Description<\/td>\nT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3\u2019- hydroxyl and 5\u2019- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.<\/td>\n<\/tr>\n
Application<\/td>\n\u2022 Cloning of restriction fragments
\n\u2022 Attaching double-stranded oligonucleotide linkers or adaptors to DNA
\n\u2022 Site-directed mutagenesis
\n\u2022 Nick repair of duplex DNA, RNA or DNA-RNA hybrids
\n\u2022 Self-circularization of linear DNA<\/td>\n<\/tr>\n
Concentration<\/td>\n1 U\/\u03bcl<\/td>\n<\/tr>\n
Format General<\/td>\nEnzyme supplied with 5X Reaction Buffer<\/td>\n<\/tr>\n
Storage Condition<\/td>\nStore all components at -20\u00b0C.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

T4 DNA Ligase | \u8ca8\u865fG467 200 U (200 \u03bcl) Cat. No. G467 Nam […]<\/p>\n","protected":false},"author":3,"featured_media":28511,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373],"tags":[5939],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44015"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44015"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44015\/revisions"}],"predecessor-version":[{"id":44017,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/44015\/revisions\/44017"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28511"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44015"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44015"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44015"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}