{"id":43899,"date":"2023-07-10T17:24:49","date_gmt":"2023-07-10T09:24:49","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43899"},"modified":"2023-07-18T11:12:19","modified_gmt":"2023-07-18T03:12:19","slug":"6-%e7%a3%b7%e9%85%b8%e6%9e%9c%e7%b3%96%e6%bf%80%e9%85%b6-pfk-%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-6-phosphofructokinasepfk-activity-assay-kit%e8%b2%a8%e8%99%9fktb1124","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=43899","title":{"rendered":"6-\u78f7\u9178\u679c\u7cd6\u6fc0\u9176 (PFK) \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | 6-Phosphofructokinase(PFK) Activity Assay Kit\u8ca8\u865fKTB1124"},"content":{"rendered":"

6-\u78f7\u9178\u679c\u7cd6\u6fc0\u9176 (PFK) \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | 6-Phosphofructokinase(PFK) Activity Assay Kit\u8ca8\u865fKTB1124<\/span><\/strong><\/a><\/span><\/p>\n

(96-well UV plateor microquartz cuvette)<\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong><\/span>\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08Phosphofructokinase , PFK\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u4e2d\u3002\u5b83\u8ca0\u8cac\u5c07\u679c\u7cd6 6-\u78f7\u9178(fructosae 6-phosphate)\u548c ATP \u8f49\u5316\u70ba\u679c\u7cd6 1,6 \u4e8c\u78f7\u9178(fructosae 1,6 diphosphate )\u548c ADP\u3002\u5b83\u662f\u7cd6\u9175(glycolysis )\u89e3\u904e\u7a0b\u4e2d\u7684\u95dc\u9375\u8abf\u7bc0\u9176\u4e4b\u4e00\u3002\u7d30\u83cc\u7d30\u80de\u4e0a\u6e05\u6db2(bacterial cell supernatant )\u548c\u5176\u4ed6\u6a23\u54c1\u4e2d\u7684\u78f7\u9178\u679c\u7cd6\u6fc0\u9176 (PFK) \u6d3b\u6027\u3002CheKine\u2122 Micro 6-\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08CheKine\u2122 Micro 6-Phosphofructokinase(PFK) Activity Assay Kit, \uff09\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u65b9\u4fbf\u3001\u5feb\u901f\u7684\u6bd4\u8272\u6cd5\u6aa2\u6e2c PFK \u7684\u65b9\u6cd5\u3002 PFK\u50ac\u5316\u679c\u7cd66-\u78f7\u9178( fructosetose 6-phosphate)\u548cATP\u751f\u6210\u679c\u7cd61\u30016-\u4e8c\u78f7\u9178( fructose-1, 6-diphosphate)\u548cADP\uff0c\u4e19\u916e\u9178\u6fc0\u9176(pyruvate kinase)\u548c\u4e73\u9178\u812b\u6c2b\u9176(lactate dehydrogenase)\u4f9d\u6b21\u9032\u4e00\u6b65\u50ac\u5316NADH\u6c27\u5316\u70baNAD+\u3002\u5728340nm\u8655\u6e2c\u91cfNADH\u4e0b\u964d\u7387\uff0c\u53cd\u6620PFK\u6d3b\u6027\u3002<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro 6-Phosphofructokinase(PFK) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nCheKine\u2122 Micro 6-Phosphofructokinase(PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022Extraction Buffer
\n\u2022 Assay Buffer
\n\u2022 Substrate Mix
\n\u2022 Enzyme 1
\n\u2022 Enzyme 2<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues\/cells
\n\u2022 Detailed sample preparation and results calculation methods are provided<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 It is advisable to measure serum potassium as soon as possible after blood collection.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

6-\u78f7\u9178\u679c\u7cd6\u6fc0\u9176 (PFK) \u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | 6-Phosphofructokinase(PFK) Act […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[5924],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43899"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43899"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43899\/revisions"}],"predecessor-version":[{"id":43901,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43899\/revisions\/43901"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43899"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43899"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43899"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}