{"id":43823,"date":"2023-07-10T14:42:35","date_gmt":"2023-07-10T06:42:35","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43823"},"modified":"2023-08-02T20:18:49","modified_gmt":"2023-08-02T12:18:49","slug":"minute-%e8%84%82%e8%82%aa%e7%b5%84%e7%b9%94-%e5%9f%b9%e9%a4%8a%e8%84%82%e8%82%aa%e7%b4%b0%e8%83%9e%e7%b8%bd%e8%9b%8b%e7%99%bd%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92-invent%e8%b2%a8","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=43823","title":{"rendered":"Minute\u2122 \u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | Invent\u8ca8\u865fAT-022"},"content":{"rendered":"<p><strong><span style=\"font-size: 14pt; color: #800000;\">\u5feb\u901f\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u7cfb\u5217-<span style=\"color: #ff9900;\">Rapid Protein Extraction Kits<\/span><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-minute_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1.jpg\" \/><\/p>\n<p><strong>Minute\u2122<\/strong><strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300eColumn Filter\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong><strong>\u00a0<\/strong><strong>\u4e00 \u5206\u9418<\/strong><strong>\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<em>vortex\u3001transfer\u3001spin \u3002<\/em><\/strong>\u907f\u514d RIPA \u4e0d\u6eb6\u6027\u86cb\u767d\u8cea\u7684\u554f\u984c\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u65b9\u6cd5\u7372\u5f97\u66f4\u5b8c\u6574\u7684\u86cb\u767d\u8cea\u8b5c\u3002Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\uff1b\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\uff0c\u7d30\u80de\u5206\u96e2\u548c\u819c\u86cb\u767d\u5206\u96e2\uff0c\u53ea\u9700\u7c21\u55ae\u56db\u6b65\u9a5f\u5373\u53ef\u5b8c\u6210\u86cb\u767d\u8cea\u8403\u53d6\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n<p><iframe loading=\"lazy\" title=\"Minute Total Protein Extraction Kit - Invent Biotechnologies\" src=\"\/\/www.youtube.com\/embed\/n0RpoqGC2IY\" width=\"562\" height=\"316\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\" data-mce-fragment=\"1\"><\/iframe><\/p>\n<p><a href=\"https:\/\/biopioneer.com.tw\/?p=43823&amp;preview=true\"><strong><span style=\"color: #33cccc; font-size: 12pt;\">Minute\u2122 \u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | Invent\u8ca8\u865fAT-022<\/span><\/strong><\/a><\/p>\n<p><span style=\"font-size: 12pt; color: #999999;\">Minute\u2122 Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Preps)<\/span><\/p>\n<p><strong><span style=\"font-size: 12pt;\"><span style=\"color: #3366ff;\"><a style=\"color: #3366ff;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/22-AT-022_v10.pdf?v=1650999022\" target=\"_blank\" rel=\"noopener\"><span style=\"font-size: 12pt;\">\u624b\u518a\u548c\u5354\u8b70<\/span><\/a><\/span><span style=\"font-size: 12pt;\">|\u00a0<span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-1_435fc4ec-c7e0-4ad8-a74f-5f8537e5d08b.pdf?14248766210075683969\" target=\"_blank\" rel=\"noopener\">\u6750\u6599\u5b89\u5168\u6578\u64da\u8868 (MSDS) | <\/a><\/span><\/span><span style=\"color: #ff0000;\"><a id=\"ui-id-2\" class=\"ui-tabs-anchor\" style=\"color: #ff0000;\" tabindex=\"-1\" role=\"presentation\" href=\"https:\/\/inventbiotech.com\/products\/minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes#tabs-3\">References (37)<\/a><\/span><\/span><\/strong><\/p>\n<p>\u8102\u80aa\u7d44\u7e54(Adipose tissue)\uff0c\u7279\u5225\u662f\u767d\u8272\u8102\u80aa\u7d44\u7e54\uff08white adipose tissue, WAT\uff09\uff0c\u9664\u4e86\u5177\u6709\u80fd\u91cf\u5132\u5b58\u529f\u80fd\u5916\uff0c\u9084\u88ab\u8a8d\u70ba\u662f\u91cd\u8981\u7684\u5167\u5206\u6ccc(endocrine)\u548c\u708e\u75c7\u5668\u5b98( inflammation organ)\u3002\u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea(adipose tissues)\u7684\u5206\u6790\u5c0d\u65bc\u4e86\u89e3\u8a31\u591a\u751f\u7406\/\u75c5\u7406\u72c0\u6cc1\u8d8a\u4f86\u8d8a\u91cd\u8981\u3002\u7136\u800c\uff0c\u7531\u65bc\u767d\u8272\u8102\u80aa\u7d44\u7e54\uff08white adipose tissue, WAT\uff09 \u548c\u68d5\u8272\u8102\u80aa\u7d44\u7e54 (brown adipose tissue, BAT) \u7684\u8102\u8cea\u542b\u91cf\u9ad8\u3001\u86cb\u767d\u8cea\u542b\u91cf\u4f4e\uff0c\u5206\u96e2\u5b83\u5011\u5728\u6280\u8853\u4e0a\u5177\u6709\u6311\u6230\u6027\u3002\u773e\u6240\u5468\u77e5\uff0c\u751f\u7269\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u6c34\u6cb9\u4e73\u6db2\u5f88\u96e3\u5206\u96e2\u3002<span style=\"font-size: 12pt;\">Minute\u2122<\/span>\u958b\u767c\u4e86\u4e00\u9805\u65b0\u6280\u8853\u4f86\u89e3\u6c7a\u9019\u500b\u554f\u984c\u3002\u63a1\u7528\u5177\u6709\u7368\u7279\u8868\u9762\u7279\u6027(unique surface property)\u548c\u9810\u5b9a\u5b54\u5f91\u548c\u539a\u5ea6\u7684\u591a\u5b54\u904e\u6ffe\u5668\uff0c\u8207\u5c08\u9580\u914d\u88fd\u7684\u7121\u53bb\u6c61\u5291\u63d0\u53d6\u7de9\u885d\u6db2\u76f8\u7d50\u5408\uff0c\u53ef\u5feb\u901f\u6709\u6548\u5730\u5206\u96e2\u4f86\u81ea\u8102\u80aa\u7d44\u7e54\u52fb\u6f3f\u7684\u6c34\u6cb9\u4e73\u6db2\u3002\u63d0\u53d6\u7de9\u885d\u6db2\u7684\u51dd\u56fa\u9ede\u6bd4\u8102\u80aa\u7d44\u7e54\u4e2d\u7684\u6cb9\u4f4e\uff0c\u4e26\u4e14\u901a\u904e\u4f7f\u7d44\u7e54\u52fb\u6f3f\u901a\u904e\u904e\u6ffe\u5668\u53ef\u4ee5\u5feb\u901f\u5730\u5c07\u6c34\u76f8\u8207\u6cb9\u76f8\u5206\u96e2\u3002\u5206\u96e2\u7684\u7e3d\u86cb\u767d\u662f\u7d44\u7e54\u4e2d\u7d30\u80de\u86cb\u767d\u7684\u516c\u6b63\u4ee3\u8868\u3002\u8207\u50b3\u7d71\u65b9\u6cd5\u76f8\u6bd4\uff0c\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u975e\u5e38\u9ad8\uff082-3 mg\/ml\uff09\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Minute%E2%84%A2-%E8%84%82%E8%82%AA%E7%B5%84%E7%B9%94%E5%9F%B9%E9%A4%8A%E8%84%82%E8%82%AA%E7%B4%B0%E8%83%9E%E7%B8%BD%E8%9B%8B%E7%99%BD%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FAT-022.webp\" \/><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Minute%E2%84%A2-%E8%84%82%E8%82%AA%E7%B5%84%E7%B9%94%E5%9F%B9%E9%A4%8A%E8%84%82%E8%82%AA%E7%B4%B0%E8%83%9E%E7%B8%BD%E8%9B%8B%E7%99%BD%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FAT-022-2.webp\" alt=\"Minute\u2122-\u8102\u80aa\u7d44\u7e54\u57f9\u990a\u8102\u80aa\u7d30\u80de\u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2-Invent\u8ca8\u865fAT-022-2.webp (480\u00d7370)\" \/><\/p>\n<p>A. \u5f9e\u4e0d\u540c\u8102\u80aa\u7d44\u7e54\u4e2d\u63d0\u53d6\u7684\u7e3d\u86cb\u767d\u7684 SDS-PAGE (10%) \u8b5c\u3002\u6cf3\u90531\uff0c\u8c6cWAT\uff1b2\u5df7\uff0c\u96deBAT\uff1b\u6cf3\u9053 3\uff0c\u5927\u9f20 WAT\u3002<\/p>\n<p>B. \u5f9e\u5927\u9f20 WAT \u4e2d\u63d0\u53d6\u7684\u86cb\u767d\u8cea\u7684\u86cb\u767d\u8cea\u5370\u8de1\u3002\u5728 8-16% \u68af\u5ea6 SDS-PAGE \u4e2d\u5206\u96e2\u86cb\u767d\u8cea\uff0c\u4e26\u7528\u4ee5\u4e0b\u7d30\u80de\u86cb\u767d\u8cea\u6a19\u8a18\u6297\u9ad4\u9032\u884c\u63a2\u6e2c\uff1a<\/p>\n<p>\u6297 Na\/K ATPase \u03b11\uff0c\u4e00\u7a2e\u8cea\u819c\u6a19\u8a18\uff08Upstate\uff0c\u514b\u9686 464.6\uff09\uff0c\u6297\u6838\u7e96\u5c64\u86cb\u767d B1\uff0c\u4e00\u7a2e\u6838\u819c\u6a19\u8a18\uff08 ab16048\uff0cabcam Cambridge\uff0cMA)\u3001\u6297\u6cdb\u9187\u7d30\u80de\u8272\u7d20 C \u9084\u539f\u9176\u6838\u5fc3\u86cb\u767d (abcam\uff0cab 96333) \u548c GAPDH\uff0c\u4e00\u7a2e\u80de\u8cea\u6a19\u8a18\u7269 (Sigma)\u3002\u901a\u904e\u5e95\u7269 Opti-4CN (Bio-RAD) \u4f7f\u7279\u5b9a\u86cb\u767d\u8cea\u689d\u5e36\u53ef\u8996\u5316\u3002<\/p>\n<p>A.\u00a0SDS-PAGE (10%) profiles of total protein extracted from different adipose\u00a0tissues. Lane 1, porcine WAT; Lane 2, chicken BAT; Lane 3, rat WAT.<\/p>\n<p>B.\u00a0Western blottings of extracted proteins from rat WAT. Proteins were separated\u00a0in 8-16% gradient SDS-PAGE and probed with following\u00a0cellular protein marker\u00a0antibodies:<\/p>\n<p>Anti-Na\/K\u00a0ATPase alpha1, a plasma membrane marker (Upstate, clone 464.6), anti-lamin B1,\u00a0a nuclear envelope marker (ab16048, abcam\u00a0Cambridge, MA),\u00a0anti-ubiquinol-cytochrome C reductase core protein (abcam, ab 96333) and GAPDH,\u00a0a cytosolic marker (Sigma). The specific\u00a0protein bands were visualized by a\u00a0substrate Opti-4CN (Bio-RAD).<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Minute\u2122 \u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291 Kit includes:<\/strong><\/span><\/p>\n<table width=\"540\">\n<tbody>\n<tr>\n<td><strong>Items<\/strong><\/td>\n<td><strong>Quantity<\/strong><\/td>\n<\/tr>\n<tr>\n<td>Buffer A (Extraction Buffer)<\/td>\n<td>15 ml<\/td>\n<\/tr>\n<tr>\n<td>Buffer B (10 X Denaturing Buffer)<\/td>\n<td>1.5 ml<\/td>\n<\/tr>\n<tr>\n<td>Buffer C (10 X Non-Denaturing Buffer)<\/td>\n<td>1.5 ml<\/td>\n<\/tr>\n<tr>\n<td>1.5 ml Microfuge Tubes<\/td>\n<td>20 units<\/td>\n<\/tr>\n<tr>\n<td>Pestles for 1.5 ml Tubes<\/td>\n<td>2 units<\/td>\n<\/tr>\n<tr>\n<td>Filter Cartridge with Collection Tubes<\/td>\n<td>20 units<\/td>\n<\/tr>\n<tr>\n<td>Protein Extraction Powder<\/td>\n<td>2 grams<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/08\/Minute%E2%84%A2-%E8%84%82%E8%82%AA%E7%B5%84%E7%B9%94-%E5%9F%B9%E9%A4%8A%E8%84%82%E8%82%AA%E7%B4%B0%E8%83%9E%E7%B8%BD%E8%9B%8B%E7%99%BD%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FAT-022.jpg\" width=\"535\" height=\"551\" \/><\/a><\/strong><\/p>\n<p><strong><a href=\"https:\/\/inventbiotech.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/strong><strong><a href=\"https:\/\/www.abmgood.com\/\"><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/a><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><img decoding=\"async\" src=\"https:\/\/www.abmgood.com\/pub\/media\/logo\/websites\/1\/abm-logo-high-res.png\" alt=\"\" \/><\/strong><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u57fa\u65bc\u65cb\u8f49\u67f1(SPIN-COLUMN-BASED )\u7684\u86cb\u767d\u8cea\u63d0\u53d6\/\u7d30\u80de\u5206\u7d1a(CELL FRACTIONATION)<\/span><\/strong><\/span><\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-4356\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2015\/02\/Compined-F.gif\" alt=\"Compined-F\" width=\"446\" height=\"167\" \/><\/strong><\/p>\n<p>Invent Biotechnologies \u6210\u7acb\u65bc 2010 \u5e74\uff0c\u56e0\u9769\u65b0\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u800c\u4eab\u8b7d\u5168\u7403\u3002\u6211\u5011\u958b\u767c\u4e86\u57fa\u65bc\u65cb\u8f49\u67f1(spin column-based)\u7684\u5275\u65b0\u6280\u8853\uff0c\u7528\u65bc\u86cb\u767d\u8cea\u7814\u7a76( protein research)\uff0c\u53ef\u7c21\u5316\u548c\u589e\u5f37\u6a23\u54c1\u88fd\u5099\u3002\u9019\u6703\u5e36\u4f86\u66f4\u9806\u66a2\u7684\u5de5\u4f5c\u6d41\u7a0b\u548c\u59cb\u7d42\u53ef\u9760\u7684\u7d50\u679c\uff0c\u5f9e\u800c\u5e36\u4f86\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002\u9078\u64c7 Minute\u2122 \u8a66\u5291\u76d2\u9032\u884c\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2(cell fractionation)\u7684\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u76f8\u4fe1\uff0c\u4ed6\u5011\u7684\u6a23\u54c1\u6e96\u78ba\u5730\u4ee3\u8868\u4e86\u6240\u7814\u7a76\u7684\u7d30\u80de\u6216\u7d44\u7e54\u7684\u81ea\u7136\u7279\u5fb5\u3002\u9019\u4e9b\u5957\u4ef6\u53ea\u9700\u8981\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\uff0c\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u5be6\u9a57\u8b8a\u91cf\u4e26\u78ba\u4fdd\u9ad8\u8cea\u91cf\u7684\u6750\u6599\u4ee5\u7372\u5f97\u66f4\u53ef\u9760\u7684\u6578\u64da\u3002Minute\u2122 \u65b9\u6cd5\u7684\u6709\u6548\u6027\u5f97\u5230\u4e86\u8d8a\u4f86\u8d8a\u591a journals\u7684\u652f\u6301 (Nature, Science, and Cell.)\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #ff0000;\"><b>The Minute\u2122 \u7684\u512a\u9ede\uff1a<\/b><\/span><br \/>\n\u2022 \u8d85\u5feb\uff08\u5feb\u81f3 1 \u5206\u9418\uff09<br \/>\n\u2022 \u6a23\u672c\u91cf\u5c0f<br \/>\n\u2022 \u6700\u5c0f\u5316\u4ea4\u53c9\u6c61\u67d3<br \/>\n\u2022 \u5167\u6e90\u6027\u57fa\u7dda\u4e0d\u8b8a<br \/>\n\u2022 \u6240\u6709\u6d3b\u9ad4\u548c\u7d44\u7e54\u985e\u578b<\/p>\n<p><span style=\"color: #ff0000;\"><strong><span style=\"font-size: 12pt;\">The Minute\u2122 \u61c9\u7528\uff1a<\/span><\/strong><\/span><br \/>\n\u2022 \u86cb\u767d\u8cea\u904b\u8f38\/\u6613\u4f4d<br \/>\n\u2022 \u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u76f8\u4e92\u4f5c\u7528<br \/>\n\u2022 \u86cb\u767d\u8cea\u4fee\u98fe<br \/>\n\u2022 \u4fe1\u865f\u8f49\u5c0e<br \/>\n\u2022 sc\/snRNA-seq<\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">Invent Minute\u2122 \u5fb9\u5e95\u6539\u8b8a\u86cb\u767d\u8cea\u6a23\u54c1\u88fd\u5099\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2<\/span><\/strong><\/p>\n<div class=\"rte\">\n<p>\u81ea 2010 \u5e74\u6210\u7acb\u4ee5\u4f86\uff0c\u6211\u5011\u7684\u9996\u8981\u76ee\u6a19\u662f\u5229\u7528\u6211\u5011\u57fa\u65bc\u65cb\u8f49\u67f1\u7684\u5275\u65b0\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u5168\u7403\u86cb\u767d\u8cea\u63d0\u53d6(protein extraction)\u548c\u7d30\u80de\u5206\u96e2(cell fractionation)\u5e02\u5834\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u8b93 Minute\u2122 \u8a66\u5291\u76d2\u6210\u70ba\u6a19\u6e96\u5be6\u9a57\u5ba4\u7a0b\u5e8f\uff0c\u53d6\u4ee3\u6240\u6709\u57fa\u65bc\u6eb6\u6db2\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u3002\u901a\u904e\u5229\u7528\u9019\u4e9b\u5957\u4ef6\uff0c\u7814\u7a76\u4eba\u54e1\u53ea\u9700\u4e00\u53f0\u53f0\u5f0f\u96e2\u5fc3\u6a5f\u5373\u53ef\u7372\u5f97\u4e00\u81f4\u7684\u7d50\u679c\u4e26\u7372\u5f97\u5353\u8d8a\u7684\u6578\u64da\u8cea\u91cf\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/5-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"491\" height=\"239\" \/><\/p>\n<p><span style=\"font-size: 12pt;\"><strong><span style=\"color: #800000;\">\u6539\u8b8a\u86cb\u767d\u8cea\u63d0\u53d6(Transforming protein extraction)<\/span><\/strong><\/span><\/p>\n<p>Minute\u2122 \u7e3d\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1( simple spin column )\uff0c\u53ef\u6709\u6548\u6d88\u9664\u7d30\u80de\u6216\u7d44\u7e54\u88c2\u89e3\u904e\u7a0b(lysis)\u4e2d\u7684\u975e\u7cfb\u7d71\u6027\u86cb\u767d\u8cea\u640d\u5931(non-systematic protein loss)\u3002\u9019\u4ee3\u8868\u8457\u76f8\u5c0d\u65bc\u50b3\u7d71\u7684\u57fa\u65bc\u89e3\u6c7a\u65b9\u6848\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982 RIPA \u7de9\u885d\u6db2(RIPA buffer)\u548c\u8d85\u8072\u8655\u7406(sonication)\u7684\u986f\u8457\u9032\u6b65\uff0c\u9019\u4e9b\u65b9\u6cd5\u4ee5\u5176\u53ef\u8b8a\u6027\u548c\u7121\u6cd5\u63d0\u4f9b\u5168\u9762\u7684\u86cb\u767d\u8cea\u8b5c\u800c\u805e\u540d\u3002\u7528\u65bc\u7e3d\u86cb\u767d\u63d0\u53d6\u7684 Minute\u2122 \u8a66\u5291\u76d2\u8207\u4f86\u81ea\u5404\u7a2e\u751f\u7269\u9ad4\u548c\u7d44\u7e54\u985e\u578b\u7684\u5c0f\u6a23\u672c\u91cf\u517c\u5bb9\uff0c\u70ba\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u672a\u6539\u8b8a\u7684\u5167\u6e90\u86cb\u767d\u57fa\u7dda( endogenous proteins)\uff0c\u5f9e\u800c\u5be6\u73fe\u66f4\u7cbe\u78ba\u7684\u5b9a\u91cf\u548c\u5206\u6790\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/Invent-Biotechs-Minute-Kit-shows-a-more-complete-cell-lysis-compared-to-RIPA-buffer-in-which-a-pellet-remains-leading-to-an-alteration-of-the-protein-profile.jpg\" width=\"374\" height=\"481\" \/><\/p>\n<p>Invent Biotech&#8217;s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/3-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"488\" height=\"243\" \/><\/p>\n<p><strong><span style=\"font-size: 12pt; color: #800000;\">\u6539\u9032\u7d30\u80de\u5206\u7d1a\u5206\u96e2(Revamping cell fractionation)<\/span><\/strong><\/p>\n<p>\u7531\u65bc\u7f3a\u4e4f\u7c21\u55ae\u7684\u5be6\u9a57\u65b9\u6848\uff0c\u50b3\u7d71\u7684\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u65b9\u6cd5\u9577\u671f\u4ee5\u4f86\u4e00\u76f4\u53d7\u5230\u7528\u6236\u504f\u5dee\u548c\u7d50\u679c\u4e0d\u4e00\u81f4\u7684\u5f71\u97ff\u3002 Minute\u2122 \u7d30\u80de\u5206\u96e2\u8a66\u5291\u76d2\u5fb9\u5e95\u6539\u8b8a\u4e86\u9019\u4e00\u904e\u7a0b\uff0c\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u5728 90 \u5206\u9418\u5167\u7372\u5f97\u9ad8\u7d14\u5ea6\u7684\u7d30\u80de\u5668\u7fa4\uff0c\u4e26\u4e14\u4f7f\u7528\u6700\u5c11\u7684\u6d17\u6ecc\u5291\u3002\u901a\u904e\u63a1\u7528\u7c21\u55ae\u7684\u65cb\u8f49\u67f1\uff0c\u9019\u4e9b\u8a66\u5291\u76d2\u7121\u9700\u5747\u8cea\u5316(homogenization)\u3001\u5bc6\u5ea6\u68af\u5ea6\u5206\u96e2(density gradient separation)\u548c\u8d85\u901f\u96e2\u5fc3\u7b49\u50b3\u7d71\u6280\u8853( ultra-centrifugation)\uff0c\u5f9e\u800c\u6e1b\u5c11\u4e86\u5be6\u9a57\u8b8a\u91cf\u7684\u6578\u91cf\u3002\u9019\u53ef\u4ee5\u9632\u6b62\u7d30\u80de\u5668\u640d\u50b7( organelle damage)\u4e26\u7522\u751f\u66f4\u4e00\u81f4\u548c\u751f\u7406\u76f8\u95dc\u7684\u7d50\u679c\u3002\u6191\u85c9\u591a\u7a2e Minute\u2122 \u8a66\u5291\u76d2\u53ef\u6eff\u8db3\u4e0d\u540c\u7684\u7d30\u80de\u5206\u7d1a(cell fractionation)\u5206\u96e2\u8981\u6c42\uff0c\u6211\u5011\u6b63\u5728\u63a8\u52d5\u86cb\u767d\u8cea\u7d44\u7814\u7a76\u7684\u8f49\u578b\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/2-Minute%E2%84%A2-%E5%9B%BA%E5%AE%9A%E5%92%8C%E5%8C%85%E5%9F%8B%E7%B5%84%E7%B9%94%E8%9B%8B%E7%99%BD%E8%B3%AA%E6%8F%90%E5%8F%96%E8%A9%A6%E5%8A%91%E7%9B%92-Invent%E8%B2%A8%E8%99%9FFE-025.jpg\" width=\"480\" height=\"238\" \/><\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>\u652f\u6301\u6240\u6709\u7814\u7a76\u5b78\u79d1(Supporting all research disciplines)<\/strong><\/span><\/p>\n<p>Minute\u2122 \u8a66\u5291\u76d2\u65e8\u5728\u7121\u7e2b\u96c6\u6210\u5230\u73fe\u6709\u7684\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d30\u80de\u5206\u7d1a\u5206\u96e2\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u63d0\u4f9b\u5feb\u901f\u4e14\u4e00\u81f4\u7684\u6027\u80fd\uff0c\u78ba\u4fdd\u53ef\u9760\u7684\u7d50\u679c\u3002\u7136\u800c\uff0c\u4e0d\u8981\u53ea\u76f8\u4fe1\u6211\u5011\u7684\u8a71\u2014\u2014\u6211\u5011\u7684\u7522\u54c1\u5df2\u5728\u79d1\u5b78\u6587\u737b\u4e2d\u88ab\u5ee3\u6cdb\u5f15\u7528\uff0c\u9019\u8868\u660e\u7814\u7a76\u754c\u5c0d\u7c21\u5316\u4e14\u53ef\u9760\u7684\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u7684\u9700\u6c42\u4e0d\u65b7\u589e\u9577\u3002\u7acb\u5373\u5207\u63db\u5230 Minute\u2122\uff0c\u9ad4\u9a57\u6211\u5011\u6280\u8853\u7684\u512a\u52e2\uff0c\u7121\u8ad6\u60a8\u7684\u7814\u7a76\u9818\u57df\u5982\u4f55\u3002<\/p>\n<table style=\"height: 2673px;\" width=\"995\">\n<tbody>\n<tr>\n<td width=\"64\">SD-001\/SN-002<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-003<\/td>\n<td>Minute <sup>TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-006<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SM-005-p<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MP-007<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-038<\/td>\n<td>Minute<sup> TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SD-008\/SN-009<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-010<\/td>\n<td width=\"667\">Minute <sup>TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CP-011<\/td>\n<td width=\"667\">Minute <sup>TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SB-004<\/td>\n<td width=\"667\">Minute<sup> TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SC-012<\/td>\n<td>Minute <sup>TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NE-013<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HP-014<\/td>\n<td width=\"667\">Minute<sup> TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YT-015<\/td>\n<td width=\"667\">Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YD-016<\/td>\n<td width=\"667\">Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">YM-017<\/td>\n<td>Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MM-018<\/td>\n<td width=\"667\">Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-019<\/td>\n<td width=\"667\">Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">HD-021<\/td>\n<td width=\"667\">MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AT-022<\/td>\n<td width=\"667\">MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AF-023<\/td>\n<td>MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NI-024<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">BN-020<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SN-047<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">AN-029<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">FE-025<\/td>\n<td>Minute<sup>TM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">MS-026<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Total Protein Extraction Kit for Mass Spectrometry (50 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">EI-027<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ED-028<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Endosome Isolation and Cell Fractionation Kit (20 Tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">SE-030<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">CS-031<\/td>\n<td>Minute<sup>TM<\/sup> Cell Suspension Isolation from Fresh\/Frozen Tissues (50 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NT-032<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">NM-033<\/td>\n<td width=\"667\">Minute<sup>TM <\/sup>Detergent-Free Nuclear Matrix Protein Extraction Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LY-034<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-035<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> High Fidelity ER Isolation Kit for Liver Tissue (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">ER-036<\/td>\n<td>Minute<sup>TM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">GO-037<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Golgi Apparatus Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-039<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">LR-042<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PT-040<\/td>\n<td width=\"667\">Minute<sup>TM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests)<\/td>\n<\/tr>\n<tr>\n<td width=\"64\">PN-041<\/td>\n<td>Minute<sup>TM<\/sup> Nuclear 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