{"id":43576,"date":"2023-07-03T18:05:23","date_gmt":"2023-07-03T10:05:23","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43576"},"modified":"2023-07-17T13:54:26","modified_gmt":"2023-07-17T05:54:26","slug":"ez-cap-reagent-ag-co-transcription-capping-reagent-apexbio%e8%b2%a8%e8%99%9fb8176","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=43576","title":{"rendered":"EZ Cap\u2122 Reagent AG | Co-transcription capping reagent \u00a0APExBIO\u8ca8\u865fB8176"},"content":{"rendered":"<p><span style=\"color: #993366;\"><strong><span style=\"font-size: 14pt;\"><span style=\"color: #800080;\">\u5171\u8f49\u9304\u52a0\u5e3d\u8a66\u5291( Co-transcription capping reagent ) <\/span><span style=\"color: #ff9900;\"><span style=\"color: #800080;\">&#8211;<span style=\"color: #ff9900;\">EZ Cap\u2122 Reagent AG<\/span><br \/>\n<\/span><\/span><\/span><\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/09\/APExBIO_logo.jpg\" width=\"370\" height=\"105\" \/><\/p>\n<p><span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/www.apexbt.com\">https:\/\/www.apexbt.com<\/a><\/span><\/p>\n<p>APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n<p><strong><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=43576&amp;preview=true\"><span style=\"font-size: 12pt;\">EZ Cap\u2122 Reagent AG Co-transcription capping reagent \u00a0APExBIO\u8ca8\u865fB8176<\/span><\/a><\/span><\/strong><\/p>\n<p>EZ Cap Reagent AG \u662f\u4e00\u7a2e\u5171\u8f49\u9304\u52a0\u5e3d\u8a66\u5291(co-transcription capping reagent )\uff0c\u7528\u65bc\u9ad4\u5916\u8f49\u9304 5&#8242; \u52a0\u5e3d mRNA \u4ee5\u7522\u751f Cap 1 \u7d50\u69cbm7G(5&#8242;)ppp(5&#8242;)(2&#8217;OMeA)pG\u3002\u6587\u737b\u986f\u793a Cap AG \u53ef\u63d0\u4f9b\u9ad8\u9054 98% \u7684\u5c01\u84cb\u6548\u7387(capping efficiency )\u3002EZ Cap AG \u9700\u8981 AG \u555f\u52d5\u5668(AG initiator)\u3002 EZ Cap AG \u901a\u5e38\u63d0\u4f9b &gt;90% \u7684\u5c01\u7aef\u6750\u6599(capped material )\uff0c\u751f\u6210\u5929\u7136\u5b58\u5728\u7684 Cap 1 \u7d50\u69cb(Cap 1 structure)\u3002EZ Cap \u8207\u91ce\u751f\u578b T7 \u805a\u5408\u9176( wild-type T7 polymerase)\u517c\u5bb9\u3002\u5efa\u8b70\u4f7f\u7528 5 mM EZ Cap \u548c rNTP\u3002\u6a21\u677f(Template )\u8a2d\u8a08\u662f\u4efb\u4f55\u8f49\u9304\u7684\u4e00\u500b\u7d44\u6210\u90e8\u5206\u3002 EZ Cap \u8a66\u5291 AG \u53ef\u8207\u7279\u5b9a\u8d77\u59cb\u5e8f\u5217 5&#8242; AG 3&#8242; \u4e00\u8d77\u4f7f\u7528\u3002\u6700\u7d42\u52a0\u84cb\u6548\u7387(capping efficiency)\u53d6\u6c7a\u65bc EZ Cap \u8a66\u5291\u3001DNA \u6a21\u677f(DNA template)\u548c\u6700\u7d42 mRNA \u5e8f\u5217\u3002 RNA \u9577\u5ea6\u548c\u9e7c\u57fa\u7d44\u6210(base composition)\u5f15\u8d77\u7684\u4e8c\u7d1a\u7d50\u69cb\u6703\u5f71\u97ff\u6700\u7d42\u7684\u52a0\u5e3d\u6548\u7387( capping efficiency)\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/3-EZ-Cap%E2%84%A2-Reagent-AG-Co-transcription-capping-reagent-APExBIO%E8%B2%A8%E8%99%9FB8176.jpg\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/09\/APExBIO_logo.jpg\" width=\"277\" height=\"67\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/2-EZ-Cap%E2%84%A2-Reagent-AG-Co-transcription-capping-reagent-APExBIO%E8%B2%A8%E8%99%9FB8176.png\" width=\"428\" height=\"579\" \/><\/p>\n<div id=\"cite\" class=\"tbar\">\n<h2><strong><span style=\"font-size: 12pt; color: #800000;\">Product Citation<\/span><\/strong><\/h2>\n<\/div>\n<div class=\"detail-table \">\n<div class=\"detail-box-cont\">\n<ul id=\"artul\">\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/36090760\" target=\"_blank\" rel=\"noopener\">1. Yu Zhang, Xiaodong Xi, et al. &#8220;Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice.&#8221; Mol Ther Nucleic Acids. 2022 Aug 13;29:657-671. PMID: 36090760<\/a><\/li>\n<li><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/35862767\" target=\"_blank\" rel=\"noopener\">2. Binfan Chen, Yuchen Chen, et al. &#8220;A Single Dose of Anti-HBsAg Antibody-Encoding mRNA-LNPs Suppressed HBsAg Expression: a Potential Cure of Chronic Hepatitis B Virus Infection.&#8221; mBio. 2022 Aug 30;13(4):e0161222. PMID: 35862767<\/a><\/li>\n<li><a href=\"https:\/\/doi.org\/10.1101\/2022.05.10.491301\" target=\"_blank\" rel=\"noopener\">3. Shudan Tan, Xue Hu, et al. &#8220;Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants.&#8221; biorxiv.<\/a><\/li>\n<li><a>4. Jing Lu, Guoliang Lu, et al. &#8220;A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice.&#8221; Cell Res. 2020;1-4.<\/a>\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32801356\" target=\"_blank\" rel=\"noopener\">PMID: 32801356<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td>Physical Appearance<\/td>\n<td>Solution<\/td>\n<\/tr>\n<tr>\n<td>Storage<\/td>\n<td>Store at -20\u00b0C or below<\/td>\n<\/tr>\n<tr>\n<td>M.Wt<\/td>\n<td>1211.62 (free acid)<\/td>\n<\/tr>\n<tr>\n<td>Formula<\/td>\n<td>C<sub>32<\/sub>H<sub>40<\/sub>N<sub>15<\/sub>Na<sub>3<\/sub>O<sub>24<\/sub>P<sub>4<\/sub>\u00a0(free acid)<\/td>\n<\/tr>\n<tr>\n<td>Synonyms<\/td>\n<td>EZ Cap\u2122 Reagent AG for co-transcriptional capping of mRNA,m7G(5&#8242;)ppp(5&#8242;)(2&#8217;OMeA)pG<\/td>\n<\/tr>\n<tr>\n<td>SDF<\/td>\n<td><a href=\"https:\/\/www.apexbt.com\/downloader\/sdf\/B8176.sdf\">Download SDF<\/a><\/td>\n<\/tr>\n<tr>\n<td>Canonical SMILES<\/td>\n<td>O=C1NC(N)=NC2=C1[N+](C)=CN2[C@@H]3[C@@H](O)[C@@H](O)[C@H](COP([O-])(OP([O-])(OP([O-])(OC[C@H]4O[C@@H](N5C=NC6=C5N=CN=C6N)[C@H](OC)[C@@H]4OP([O-])(OC[C@H]7O[C@@H](N8C=NC9=C8N=C(N)NC9=O)[C@H](O)[C@@H]7O)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+]<\/td>\n<\/tr>\n<tr>\n<td>Shipping Condition<\/td>\n<td>Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.<\/td>\n<\/tr>\n<tr>\n<td>General tips<\/td>\n<td>For obtaining a higher solubility, please warm the tube at 37\u00b0C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/07\/1-EZ-Cap\u2122-Reagent-AG-Co-transcription-capping-reagent-APExBIO\u8ca8\u865fB8176.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/09\/APExBIO_logo.jpg\" width=\"370\" height=\"105\" \/><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\"><a style=\"color: #800000;\" href=\"http:\/\/www.apexbt.com\/\">APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n<p><span style=\"color: #ff9900;\"><a style=\"color: #ff9900;\" href=\"https:\/\/www.apexbt.com\">https:\/\/www.apexbt.com<\/a><\/span><\/p>\n<p>APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5171\u8f49\u9304\u52a0\u5e3d\u8a66\u5291( Co-transcription capping reagent ) &#8211;EZ C [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":34299,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[5899],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43576"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=43576"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43576\/revisions"}],"predecessor-version":[{"id":44065,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/43576\/revisions\/44065"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34299"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=43576"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=43576"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=43576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}