{"id":43398,"date":"2023-06-26T11:40:01","date_gmt":"2023-06-26T03:40:01","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=43398"},"modified":"2023-06-27T11:29:32","modified_gmt":"2023-06-27T03:29:32","slug":"%e4%ba%ba%e5%88%9d%e4%bb%a3%e8%a6%96%e7%b6%b2%e8%86%9c%e5%be%ae%e8%a1%80%e7%ae%a1%e5%85%a7%e7%9a%ae%e7%b4%b0%e8%83%9e-human-primary-retinal-microvascular-endothelial-cells-%e8%b2%a8%e8%99%9ft4169","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=43398","title":{"rendered":"\u4eba\u521d\u4ee3\u8996\u7db2\u819c\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de | Human Primary Retinal Microvascular Endothelial Cells (\u8ca8\u865fT4169)"},"content":{"rendered":"

A<\/strong>bmgood\u521d\u4ee3<\/strong>\u7cfb\u5217\u5546\u54c1<\/strong> (Primary cell)-\u4eba\u521d\u4ee3\u8996\u7db2\u819c\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de<\/strong><\/span><\/p>\n

\u4eba\u521d\u4ee3\u8996\u7db2\u819c\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de- Human Primary Retinal Microvascular Endothelial Cells (\u8ca8\u865fT4169)<\/span><\/a><\/span><\/strong><\/p>\n

Human Primary Retinal Microvascular Endothelial Cells\"\"<\/p>\n

\u4eba\u521d\u4ee3\u8996\u7db2\u819c\u5fae\u8840\u7ba1\u5167\u76ae\u7d30\u80de\u7279\u6027(Primary)<\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u578b\u614b: Adherent\u3002 \u4f86\u6e90Eye\u3002Expression Profile vWF, CD31\u3002\u89e3\u51cd\u5f8c\uff0c\u7d30\u80de\u9700\u89814-5\u5929\u624d\u80fd\u6062\u5fa9\u57f9\u990a<\/span>\u3002 \u7d30\u80de\u57f9\u990a\u689d\u4ef6: \u7d30\u80de\u5fc5\u9808\u5728\u5857\u6709\u660e\u81a0 (Gelatin Coating Solution (0.1%)\uff0cTM063)\u7684\u5e73\u677f\u4e0a\u751f\u9577\u3002\u660e\u81a0\u6eb6\u6db2 (0.1%)\u3002\u57f9\u990a\u6db2Prigrow X series medium\u00a0(<\/u>\u8ca8\u865f<\/u> TM4169<\/a><\/u>)<\/u>\u3002\u4e8c\u6c27\u5316\u78b3\uff08CO2\uff09\uff1a5\uff05\uff0c\u6eab\u5ea6\uff1a37.0\u2103\u3002\u5fc5\u9700\u8cfc\u8cb70.1% Gelatin Coating Solution (\u8ca8\u865fTM063<\/span>)\uff0c\u57f9\u990a\u6db2Prigrow X series medium\u00a0(<\/u>\u8ca8\u865fTM4169<\/a><\/u>)<\/u><\/span>\u3002<\/p>\n

\u521d\u4ee3\u7d30\u80de(Primary cells)\u8207\u6c38\u751f\u5316\u7d30\u80de\u7cfb(immortalized cell lines)\u7684\u4e0d\u540c\u4e4b\u8655\u5728\u65bc\u6709\u9650\u7684\u7fa4\u9ad4\u500d\u589e(population doubling)\u3002\u5728\u6c92\u6709\u907a\u50b3(genetic)\u548c\u5316\u5b78\u4fee\u98fe(chemical modifications)\u7684\u60c5\u6cc1\u4e0b\uff0c\u521d\u4ee3\u7d30\u80de(primary cells)\u4fdd\u7559\u4e86\u5176\u8d77\u6e90\u7d44\u7e54\u7cfb\u7d71\u7684\u8a31\u591a\u91cd\u8981\u751f\u7406\u7279\u6027\uff0c\u4e26\u5bc6\u5207\u6a21\u64ec\u9ad4\u5167\u689d\u4ef6\u3002\u56e0\u6b64\uff0c\u5b83\u5011\u70ba\u5f9e\u57fa\u790e\u7d30\u80de\u751f\u7269\u5b78(cell biology)\u548c\u751f\u7406\u5b78(physiology)\u3001\u767c\u80b2\u751f\u7269\u5b78(developmental biology)\u3001\u75be\u75c5\u6a5f\u5236(disease mechanisms)\u5230\u85e5\u7269\u7be9\u9078(drug screening)\u548c\u6cbb\u7642\u958b\u767c(therapeutics development)\u7684\u5404\u7a2e\u7814\u7a76\u63d0\u4f9b\u4e86\u7406\u60f3\u7684\u7d30\u80de\u6a21\u578b\u3002<\/div>\n
\n
Specifications<\/span><\/strong><\/span><\/div>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n
Cat. No.<\/td>\nT4169<\/td>\n<\/tr>\n
Name<\/td>\nHuman Primary Retinal Microvascular Endothelial Cells<\/td>\n<\/tr>\n
Category<\/td>\nPrimary Cells<\/td>\n<\/tr>\n
Cell Type<\/td>\nPrimary Cells<\/td>\n<\/tr>\n
Organism<\/td>\nHuman (H. sapiens)<\/td>\n<\/tr>\n
Tissue<\/td>\nEye<\/td>\n<\/tr>\n
Donor Disease<\/td>\nNormal<\/td>\n<\/tr>\n
Growth Properties<\/td>\nAdherent<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

HOT!!<\/strong><\/span>\u00a0<\/u><\/a><\/span>\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/a>\u00a0|<\/strong>\u00a0DAPI\u67d3\u6838<\/a>\u00a0|<\/strong>\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|<\/strong>\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|<\/strong>\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n

\"abm\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

Abmgood\u4e0d\u673d\u5316\u7cfb\u5217\u5546\u54c1\u00a0(immortalized reagenet)\u00a0Ready-to-use<\/span><\/strong><\/span><\/p>\n

Abmgood\u516c\u53f8\u65bc\u4eba\u985e\u8207\u8001\u9f20\u4e0d\u673d\u4e4b\u7d30\u80de\u682a\u4e0a\uff0c\u5177\u6709\u5e7e\u5341\u5e74\u7684\u5be6\u9a57\u7d93\u9a57\uff1b\u63d0\u4f9b\u4e00\u7cfb\u5217\u4e0d\u673d (immortalized)\u5316\u4e4b\u7d30\u80de\u682a\u3001\u53ca\u4e0d\u673d\u5316\u4e4b\u8a66\u5291\u5546\u54c1\u3002ready-to-use\u5b9a\u91cf\u597d\u4e4bretrovirus\u3001lentiviru\u3001 adenovirus\uff0c\u53ef\u76f4\u63a5\u52a0\u65bc\u7d30\u80de\u4e2d\u9032\u884c\u611f\u67d3\u8868\u73fe\uff1b\u75c5\u6bd2\u6db2\u5305\u62ec\u6709SV40\u91cd\u7d44\u75c5\u6bd2\u3001EBV\u91cd\u7d44\u75c5\u6bd2\u3001hTERT\u75c5\u6bd2\u6db2\u3001Myc\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001p53\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Rb\u57fa\u56e0\u75c5\u6bd2\u6db2\u3001Ras\u57fa\u56e0\u75c5\u6bd2\u6db2\u3002\u63d0\u4f9b106\u53ca109\u5169\u7a2e\u5305\u88dd\u529b\u50f9\u4e4b\u75c5\u6bd2\u6db2\uff1b\u4ea6\u63d0\u4f9b\u4e00\u7cfb\u5217\u75c5\u6bd2\u529b\u50f9\u6fc3\u7e2e\u8207\u5b9a\u91cf\u8a66\u5291\u5546\u54c1\u3002<\/p>\n

\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002\u8207\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\uff1b\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n

A<\/strong>bmgood<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316\u7cfb\u5217\u5546\u54c1<\/strong>\u00a0(immortalized cell)<\/strong><\/span><\/p>\n

\u6839\u64da\u7d30\u80de\u985e\u578b\uff0cPrimary cells\u521d\u4ee3\u7d30\u80de\u5728\u9ad4\u5916\u5177\u6709\u5c0f\u65bc10-50\u6b21\u50b3\u4ee3\u7684\u6709\u9650\u58fd\u547d\u3002 \u4e0d\u673d\u5316\u7d30\u80de\u682a\u80fd\u5920\u5ef6\u9577\u589e\u6b96\uff0c\u4e26\u4e14\u5177\u6709\u8207\u89aa\u4ee3\u7d44\u7e54\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u57fa\u56e0\u578b\u548c\u8868\u73fe\u578b\u3002<\/p>\n

\"cell-biology-tab-final<\/p>\n

\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684<\/strong>\u4e0d<\/strong>\u673d<\/strong>\u5316Immortalized\u7b56\u7565<\/strong><\/span><\/p>\n

\u5b58\u5728\u7528\u65bc\u5728\u57f9\u990a\u7269\u4e2d\u4e0d<\/strong>\u673d<\/strong>\u5316<\/strong>\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u7684\u5e7e\u7a2e\u65b9\u6cd5\u3002 Abmgood\u591a\u5e74\u7814\u767c\u7d93\u9a57\uff0c\u958b\u767c\u51fa\u4e86\u4e00\u7cfb\u5217Immortalized\u5546\u54c1\uff1b\u5305\u62ec\u5373\u7528\u578b\u9006\u8f49\u9304\u75c5\u6bd2\uff0c\u6162\u75c5\u6bd2\u548c\u817a\u75c5\u6bd2\u8f09\u9ad4\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene Overexpressed<\/strong><\/td>\nViruses Offered<\/strong><\/td>\nApplication<\/strong><\/td>\n<\/tr>\n
hTERT<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u53ef\u80fd\u5728\u4e00\u4e9b\u7d30\u80de\u985e\u578b\uff08\u4f8b\u5982\u4e0a\u76ae\u7d30\u80de\uff09\u4e2d\u5f15\u8d77\u7d30\u80de\u6b7b\u4ea1<\/td>\n<\/tr>\n
SV40 T Antigen<\/a><\/td>\nAdenovirus,
\nLentivirus,
\nRetrovirus<\/td>\n		\u53ef\u9760\u548c\u5e38\u7528\u3002 \u901a\u5e38\u7528\u65bc\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
EBV genes<\/a><\/td>\nEpstein-Barr Virus<\/td>\n\u4e0d\u673d\u5316B\u6dcb\u5df4\u7d30\u80de\uff0c\u6709\u6642\u662fT\u6dcb\u5df4\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HPV E6-E7<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u89d2\u5316\u7d30\u80de\u3002<\/td>\n<\/tr>\n
HOXB8, HOXA9, HOXA10<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u9020\u8840\u7d30\u80de\uff0c\u5305\u62ec\u5de8\u566c\u7d30\u80de\uff0c\u9020\u8840\u7956\u7d30\u80de\u548c\u9aa8\u9ad3\u7956\u7d30\u80de\u3002<\/td>\n<\/tr>\n
Adenoviral E1A-E1B<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5927\u7bc4\u570d\u7684\u521d\u7d1a\u56d3\u9f52\u52d5\u7269\u7d30\u80de\uff0c\u5305\u62ec\u7f8a\u6c34\u7d30\u80de\uff0c\u539f\u767c\u6027\u4e0a\u76ae\u7d30\u80de\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u548c\u539f\u4ee3\u814e\u7d30\u80de\u3002<\/td>\n<\/tr>\n
p53, Rb siRNA<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u8868\u9054\u4ee5\u66f4\u597d\u5730\u4f7f\u4e0a\u76ae\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/td>\n<\/tr>\n
Bmi1<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u4e0a\u76ae\u7d30\u80de\u3002<\/td>\n<\/tr>\n
CDK4<\/a><\/td>\nLentivirus<\/td>\n\u8207hTERT\u5171\u540c\u8868\u9054\u66f4\u771f\u5be6\u7684\u7d30\u80de\u6a21\u578b\u3002<\/td>\n<\/tr>\n
Myc T58A (c-Myc), Ras V12 (H-Ras)<\/a><\/td>\nLentivirus<\/td>\n\u4e0d\u673d\u5316\u5404\u7a2e\u7d30\u80de\uff0c\u5305\u62ec\u795e\u7d93\u5e79\u7d30\u80de\uff0c\u521d\u4ee3\u814e\u7d30\u80de\uff0c\u6210\u7d05\u7d30\u80de\u548c\u5404\u7a2e\u4e0a\u76ae\u7d30\u80de\uff08\u539f\u767c\u6027\u524d\u5217\u817a\uff0c\u4e73\u817a\uff0c\u6c23\u9053\u7b49\uff09\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n