{"id":42992,"date":"2023-06-09T18:44:23","date_gmt":"2023-06-09T10:44:23","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42992"},"modified":"2023-06-13T17:51:37","modified_gmt":"2023-06-13T09:51:37","slug":"superkine-lipo3-0%e9%ab%98%e6%95%88%e8%bd%89%e6%9f%93%e8%a9%a6%e5%8a%91-lipo3-0-efficient-transfection-reagent%e8%b2%a8%e8%99%9fbmu111-en","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42992","title":{"rendered":"SuperKine\u2122 Lipo3.0\u9ad8\u6548\u8f49\u67d3\u8a66\u5291 | Lipo3.0 Efficient Transfection Reagent\u8ca8\u865fBMU111-EN"},"content":{"rendered":"

SuperKine\u2122 Lipo3.0\u9ad8\u6548\u8f49\u67d3\u8a66\u5291| Lipo3.0 Efficient Transfection Reagent Abbkine\u8ca8\u865fBMU111-EN<\/strong><\/a><\/span><\/p>\n

new cationic liposome transfection reagent<\/p>\n

<\/p>\n

SuperKine\u2122 Lipo3.0\u9ad8\u6548\u8f49\u67d3\u8a66\u5291\u662f\u4e00\u7a2e\u9ad8\u6548\u7684\u65b0\u578b\u967d\u96e2\u5b50\u8102\u8cea\u9ad4\u8f49\u67d3\u8a66\u5291(new cationic liposome transfection reagent )\uff0c\u9069\u7528\u65bc\u591a\u7a2e\u52d5\u7269\u7d30\u80de\u8cea\u9ad4DNA(plasmid DNA)\u548csiRNA\u8f49\u67d3\uff0c\u5c0d\u5927\u591a\u6578\u52d5\u7269\u7d30\u80de\u5177\u6709\u8f03\u9ad8\u7684\u8f49\u67d3\u6548\u7387(transfection efficiency )\u3002SuperKine\u2122 Lipo3.0 Efficient Transfection Reagent is a highly efficient new cationic liposome transfection reagent suitable for plasmid DNA and siRNA transfection in various animal cells, with high transfection efficiency for most animal cells.<\/p>\n

<\/p>\n

Specification<\/span><\/strong><\/span><\/h3>\n\n\n\n\n
Product name<\/td>\nSuperKine\u2122 Lipo3.0 Efficient Transfection Reagent<\/a><\/td>\n<\/tr>\n
Applications notes<\/td>\nSuperKine\u2122 Lipo3.0 Efficient Transfection Reagent is a highly efficient new cationic liposome transfection reagent suitable for plasmid DNA and siRNA transfection in various animal cells, with high transfection efficiency for most animal cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/h3>\n

Product Properties<\/span><\/strong><\/span><\/h3>\n\n\n\n\n\n\n\n\n\n
Formulation<\/td>\nLiquid solution<\/td>\n<\/tr>\n
Kit components<\/td>\nTransfection Reagent<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 High transfection efficiency: The staining efficiency of conventional animal cells, such as HEK293, HeLa, etc. is as high as 90-95%.
\n\u2022Strong compatibility: it is not affected by serum and the medium no need to be changed before and after transfection.<\/td>\n<\/tr>\n
Usage notes<\/td>\nThaw all the components at room temperature before opening<\/td>\n<\/tr>\n
Storage instructions<\/td>\nStored at 4\u2103 for 12 months, avoid freezing<\/td>\n<\/tr>\n
Shipping<\/td>\nBlue ice<\/td>\n<\/tr>\n
Precautions<\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

A.Transfection of DNA <\/span><\/strong><\/span><\/p>\n

1.Cells were grown in suitable well plates or dish esone day before transfection to a density of 70-80% at the time of transfection.<\/p>\n

2.Replace with fresh culture medium before transfection.<\/p>\n

3.Preparation of Transfectionreagent\/DNAcomplex:<\/p>\n

(1)DNA-Opti-MEM: Add 25\u03bcL Opti-MEM or other serum-free, antibiotic-free cell medium to a 1.5mL sterile EP tube, then add 0.5 \u03bcg DNA to the tube (Asanexample,0.5mg DNA was transfected ineach well of 24-well plate,refertoTable1), gently mix with a pipette. (2)Transfectionreagent-Opti-MEM:Add 25\u03bcL Opti-MEM or other serum-free, antibiotic-free cell medium to another 1.5mL terile EP tube, then add1.5\u03bcL Transfect ion reagent to the tube (1-5\u03bcL\/\u03bcgDNA,3\u03bcL\/\u03bcgDNAisrecommended), gentlymix with a pipette. (3)Add the Transfection reagent-Opti-MEMtoDNA-Opti-MEM, gently mix with apipette, afters tandingat room temperature for 10-15min,it can be used for transfection.<\/p>\n

4.Transfection:Dropthe Transfection reagent\/DNAcomplex into the culture medium and gently shake the culture plate or dish to even lydisperse the Transfection reagent\/DNA.<\/p>\n

<\/p>\n

B. Transfection of siRNA<\/span><\/strong><\/span>
\n1. Cells were grown in suitable well plates or dishes one day before transfection to a density of 70-80% at the time of transfection.<\/p>\n

2. Replace with fresh culture medium before transfection.<\/p>\n

3. Preparation of siRNA: Prepare siRNA into a 20 \u03bcM storage solution using RNase-free H2O.<\/p>\n

4. Preparation of Transfection reagent\/siRNA complex:<\/p>\n

(1) siRNA-Opti-MEM: Add 25 \u03bcL Opti-MEM or other serum-free, antibiotic-free cell medium to a 1.5 mL sterile RNase-freeEPtube, then add 1 \u03bcL 20 \u03bcM siRNA storage solutionto the tube (As an example, 20 pmol siRNA was transfected ineachwell of24-well plate, refer to Table 2), gently mix with a pipette.<\/p>\n

(2) Transfection reagent-Opti-MEM: Add 25 \u03bcL Opti-MEM or other serum-free, antibiotic-free cell mediumto another 1.5mLsterileRNase-free EP tube, then add 1.2 \u03bcL Transfection reagent to the tube (0.4-1 \u03bcL\/10 pmol siRNA, 0.6 \u03bcL\/10 pmol siRNAisrecommended), gently mix with a pipette.<\/p>\n

(3) Add the Transfection reagent-Opti-MEM to siRNA-Opti-MEM, gently mix with a pipette, after standing at roomtemperaturefor10-15 min, it can be used for transfection.<\/p>\n

<\/p>\n

5. Transfection: Drop the Transfection reagent\/siRNA complex into the culture medium and gently shake the culture plateor dish to evenly disperse the Transfection reagent\/siRNA.<\/p>\n

6. Continue to culture for 24-72 h and test with<\/p>\n","protected":false},"excerpt":{"rendered":"

SuperKine\u2122 Lipo3.0\u9ad8\u6548\u8f49\u67d3\u8a66\u5291| Lipo3.0 Efficient Transfectio […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[5831],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42992"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42992"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42992\/revisions"}],"predecessor-version":[{"id":54581,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42992\/revisions\/54581"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42992"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}