{"id":42881,"date":"2023-06-06T11:57:27","date_gmt":"2023-06-06T03:57:27","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42881"},"modified":"2023-11-23T15:08:04","modified_gmt":"2023-11-23T07:08:04","slug":"ez-cap-cy5-egfp-mrna-5-moutp-apexbio%e8%b2%a8%e8%99%9f-r1011","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42881","title":{"rendered":"EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) APExBIO\u8ca8\u865f R1011"},"content":{"rendered":"

EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) APExBIO\u8ca8\u865f R1011<\/strong><\/span><\/a><\/span><\/p>\n

EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP)<\/span><\/strong><\/span>\u7522\u54c1\u4ecb\u7d39<\/span><\/strong><\/span><\/p>\n

\u5177\u6709 Cap 1 \u7d50\u69cb\u7684 EGFP mRNA\uff0c\u7531 5-moUTP \u548c Cy5-utp \u4fee\u98fe\uff0c\u63d0\u4f9b\u66f4\u9ad8\u7684\u8f49\u9304\u6548\u7387\u4e26\u6291\u5236 RNA \u4ecb\u5c0e\u7684\u5148\u5929\u514d\u75ab\u6fc0\u6d3b\u3002EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) \u4e00\u65e6\u9032\u5165\u6700\u521d\u5f9e\u6c34\u6bcd (Aequorea victoria) \u5206\u96e2\u7684\u7d30\u80de\uff0c\u5c31\u6703\u8868\u9054\u589e\u5f37\u578b\u7da0\u8272\u87a2\u5149\u86cb\u767d (EGFP)\u3002\u60a8\u53ef\u4ee5\u5728 509 nm \u8655\u89c0\u5bdf\u5230\u7da0\u8272\u87a2\u5149\u3002 EGFP \u901a\u5e38\u7528\u4f5c\u57fa\u56e0\u8abf\u63a7\u548c\u529f\u80fd\u7814\u7a76\u7684\u5831\u544a\u57fa\u56e0\u3002\u9069\u7528\u65bc mRNA \u50b3\u905e( mRNA delivery)\u3001\u7ffb\u8b6f\u6548\u7387(cell viability)\u3001\u7d30\u80de\u6d3b\u529b(cell viability)\u548c\u9ad4\u5167\u6210\u50cf\u7b49\u6aa2\u6e2c\u3002EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) \u7684\u6fc3\u5ea6\u70ba 1mg\/ml\u3002\u5b83\u7531 EZ Cap\u2122 Reagent AG (3′ OMe)\uff08\u8ca8\u865f B8178\uff09\u5171\u8f49\u9304\u5c01\u7aef\uff0c\u53ef\u9ad8\u6548\u751f\u6210\u5e3d 1 \u7d50\u69cb\u3002 Cap 1 \u7d50\u69cb\u66f4\u9069\u5408\u54fa\u4e73\u52d5\u7269\u7cfb\u7d71\uff0c\u8f49\u9304\u6548\u7387\u9ad8\u65bc Cap 0 \u7d50\u69cb\uff08ARCA \u548c mCap\uff09\u3002 EZ Cap\u2122 Reagent AG (3′ OMe) \u7372\u5f97\u4e86\u50c5\u4ee5\u6b63\u78ba\u65b9\u5411\u63d2\u5165\u7684\u80fd\u529b\uff0c\u5c0e\u81f4\u5f62\u6210\u7684 mRNA \u7684\u7ffb\u8b6f\u6548\u7387\u662f\u4f7f\u7528 EZ Cap\u2122 Reagent AG \u555f\u52d5\u7684 mRNA \u7684\u5169\u500d\u3002\u6dfb\u52a0 5-moUTP (5-Methoxy-UTP) \u548c poly(A) \u5c3e\u5df4\u53ef\u6291\u5236 RNA \u4ecb\u5c0e\u7684\u5148\u5929\u514d\u75ab\u6fc0\u6d3b\u4e26\u589e\u52a0 mRNA \u5728\u9ad4\u5916\u548c\u9ad4\u5167\u7684\u7a69\u5b9a\u6027\u548c\u58fd\u547d\u3002 Poly(A) \u5c3e\u5728\u63d0\u9ad8\u7ffb\u8b6f\u8d77\u59cb\u6548\u7387\u65b9\u9762\u4e5f\u8d77\u8457\u91cd\u8981\u4f5c\u7528\u3002 Cy5 \u662f\u4e00\u7a2e\u5408\u6210\u7684\u7d05\u8272\u87a2\u5149\u67d3\u6599\uff0c\u6700\u5927\u6fc0\u767c\u548c\u767c\u5c04\u6ce2\u9577\u5206\u5225\u70ba 650 nm \u548c 670 nm\u3002 Cy5 EGFP mRNA \u662f\u78ba\u5b9a\u7368\u7acb\u65bc\u7ffb\u8b6f\u7684 mRNA \u905e\u9001\u548c\u5b9a\u4f4d\u7684\u7406\u60f3\u5206\u5b50\u3002 Cy5-UTP \u548c 5-moUTP \u5728\u8f49\u9304\u6642\u4ee5 1:3 \u7684\u6bd4\u4f8b\u4f7f\u7528\u3002\u6309\u6b64\u6bd4\u4f8b\u9032\u884c\u66ff\u63db\u6703\u7522\u751f\u6613\u65bc\u53ef\u8996\u5316\u4e14\u4ecd\u53ef\u5728\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u7ffb\u8b6f\u7684 mRNA\u3002\u7c97\u7565\u5730\u8aaa\uff0c\u7ffb\u8b6f\u6548\u7387\u548c Cy5-UTP \u66ff\u63db\u4e4b\u9593\u5b58\u5728\u53cd\u76f8\u95dc\u3002\u6240\u6709\u7684\u4fee\u6539\u90fd\u662f\u70ba\u4e86\u6a21\u4eff\u4e00\u500b\u5b8c\u5168\u52a0\u5de5\u7684\u6210\u719f mRNA\u3002 EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) \u662f\u89c0\u5bdf mRNA \u50b3\u905e(mRNA delivery)\u3001\u5b9a\u4f4d(localization)\u3001\u7ffb\u8b6f( translation )\u548c\u5176\u4ed6\u884c\u70ba\u7684\u7406\u60f3\u7522\u54c1\u3002<\/p>\n

EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation<\/p>\n

<\/p>\n

\"COA\u6587\u4ef6_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n
mRNA Length<\/td>\n996 nucleotides<\/td>\n<\/tr>\n
Concentration<\/td>\n1 mg\/mL<\/td>\n<\/tr>\n
Buffer<\/td>\n1 mM Sodium Citrate, pH 6.4<\/td>\nStorage<\/td>\n-40\u00b0C or below<\/td>\n<\/tr>\n
General tips<\/td>\nDissolve it on ice and take care to prevent RNase contamination degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For the first time, it is gently centrifuged and divided into several parts for stand-alone use. Use RNase-free reagents and consumables, using appropriate RNase-free technology. It can not be added to the serum-containing medium until it is mixed with the transfection reagent.<\/td>\n<\/tr>\n
Shipping Condition<\/td>\nEvaluation sample solution : ship with dry ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/span><\/p>\n

https:\/\/www.apexbt.com<\/a><\/span><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

EZ Cap\u2122 Cy5 EGFP mRNA (5-moUTP) APExBIO\u8ca8\u865f R1011 EZ Cap\u2122 […]<\/p>\n","protected":false},"author":3,"featured_media":34299,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[5801],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42881"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42881"}],"version-history":[{"count":7,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42881\/revisions"}],"predecessor-version":[{"id":47300,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42881\/revisions\/47300"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34299"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42881"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42881"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42881"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}