{"id":42794,"date":"2023-06-01T12:00:46","date_gmt":"2023-06-01T04:00:46","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42794"},"modified":"2023-06-05T18:48:41","modified_gmt":"2023-06-05T10:48:41","slug":"%e7%b4%b0%e8%83%9e%e6%af%92%e6%80%a7%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e6%af%94%e8%89%b2%e6%b3%95%ef%bc%89-abcam-%e8%b2%a8%e8%99%9fab112118","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42794","title":{"rendered":"\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09| Abcam \u8ca8\u865fab112118"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=42794&amp;preview=true\"><span style=\"font-size: 12pt;\"><strong>\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09<\/strong><\/span><span style=\"font-size: 12pt;\"><strong>| Abcam \u8ca8\u865f<\/strong><\/span><\/a><strong><a style=\"color: #33cccc;\" href=\"https:\/\/biopioneer.com.tw\/?p=42467&amp;preview=true\">ab112118<\/a><\/strong><\/span><\/p>\n<p>Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)<\/p>\n<p><strong><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E7%90%86%E4%BB%8B%E7%B4%B9-300x114.png\" alt=\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"93\" height=\"35\" \/><\/strong><\/p>\n<p>Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.<\/p>\n<p>\u76e3\u6e2c\u7d30\u80de\u6bd2\u6027( cell cytotoxicity)\u662f\u7814\u7a76\u7d30\u80de\u529f\u80fd\u6700\u91cd\u8981\u7684\u4efb\u52d9\u4e4b\u4e00\u3002\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09( Abcam \u8ca8\u865fab112118) \u4f7f\u7528\u5c08\u6709\u7684\u6c34\u6eb6\u6027\u67d3\u6599\uff0c\u53ef\u5728\u7d30\u80de\u6e1b\u5c11\u6642\u6539\u8b8a\u5176\u5438\u6536\u5149\u8b5c\u3002\u5438\u6536\u7387\u7684\u8b8a\u5316\u8207\u6d3b\u7d30\u80de\u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002 \u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u5177\u9ad8\u9748\u654f\u5ea6\u3001\u975e\u653e\u5c04\u6027\u548c\u514d\u6d17\u6cd5\u7b49\u7279\u9ede\uff0c\u9069\u7528\u65bc\u7d30\u80de\u589e\u6b96\u6216\u5c0d\u591a\u7a2e\u5316\u5408\u7269\u7684\u7d30\u80de\u6bd2\u6027\u7684\u9ad8\u901a\u91cf\u7be9\u9078\u3002 \u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u4e0d\u9700\u8981\u9810\u5148\u6df7\u5408\u7d44\u5206\uff0c\u4e26\u4e14\u8207\u57fa\u65bc\u56db\u5511\u7684\u6bd4\u8272\u6e2c\u5b9a\uff08\u4f8b\u5982 MTT \u548c XTT\uff09\u76f8\u6bd4\u5177\u6709\u66f4\u9ad8\u7684\u9748\u654f\u5ea6\u3002\u5b83\u914d\u5099\u7684\u8a66\u5291\u8db3\u4ee5\u9032\u884c 1000 \u6b21\u6aa2\u6e2c\u3002\u8a66\u5291\u76d2\u6210\u5206\u975e\u5e38\u7a69\u5b9a\uff0c\u7d30\u80de\u6bd2\u6027\u6975\u5c0f\uff0c\u56e0\u6b64\u5982\u679c\u9700\u8981\uff0c\u53ef\u4ee5\u5ef6\u9577\u5b75\u80b2\u6642\u9593\uff08\u4f8b\u5982 24-48 \u5c0f\u6642\uff09\u3002\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\u5805\u56fa\u8010\u7528\u4e14\u4f7f\u7528\u65b9\u4fbf\u3002\u5b83\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u9069\u61c9\u5404\u7a2e\u5100\u5668\u5e73\u53f0\u3002\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u5728\u65b9\u4fbf\u7684 96 \u5b54\u6216 384 \u5b54\u5fae\u91cf\u6ef4\u5b9a\u677f\u683c\u5f0f\u4e2d\u9032\u884c\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/%E7%B4%B0%E8%83%9E%E6%AF%92%E6%80%A7%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91ABCAM.jpg\" \/><\/p>\n<p><span style=\"color: #800000;\"><strong><span style=\"font-size: 12pt;\">\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/strong><\/span><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/112\/ab112118\/Images\/ab112118-236463-cell-cytotoxicity-assay-kit-colorimetric-sensitivity.jpg\" alt=\"\u529f\u80fd\u7814\u7a76 - \u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab112118)\" \/><\/p>\n<p>\u2022HeLa\uff1a\u5be6\u9a57\u524d\u4e00\u5929\u7d30\u80de\u4ee596 wp @ 1e4 \u7d30\u80de\/\u5b54\u63a5\u7a2e\u4e26\u75281\u00b5M\u300110\u00b5M \u548c50\u00b5M \u559c\u6a39\u9e7c\u8655\u7406\uff08\u5b75\u80b224 \u5c0f\u6642\uff09\uff1b\u5be6\u9a57\u7576\u5929\u7d30\u80de\u8207 Assay Solution \u5b75\u80b2 2 \u5c0f\u6642\u3002<\/p>\n<p>\u2022NIH3T3\uff1a\u5be6\u9a57\u524d\u4e00\u5929\u7d30\u80de\u4ee596 wp @ 1e4 \u7d30\u80de\/\u5b54\u63a5\u7a2e\u4e26\u75281\u00b5M\u300110\u00b5M \u548c50\u00b5M \u559c\u6a39\u9e7c\u8655\u7406\uff08\u5b75\u80b224 \u5c0f\u6642\uff09\uff1b\u5be6\u9a57\u7576\u5929\u7d30\u80de\u8207 Assay Solution \u5b75\u80b2 2 \u5c0f\u6642\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.abcam.com\/ps\/products\/112\/ab112118\/Images\/ab112118-1174-cell-cytotoxicity-assay-kit-colorimetric-sample.jpg\" alt=\"\u529f\u80fd\u7814\u7a76 - \u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab112118)\" \/><\/p>\n<p>\u4f7f\u7528 ab112118 \u6e2c\u91cf CHO-K1 \u7d30\u80de\u6578\u91cf\u53cd\u61c9\u3002\u5c07 CHO-K1 \u7d30\u80de\u4ee5 0 \u81f3 10,000 \u500b\u7d30\u80de\/\u5b54\/100 \u00b5L \u7684\u6fc3\u5ea6\u63a5\u7a2e\u5728\u9ed1\u58c1\/\u900f\u660e\u5e95\u90e8\u7684 96 \u5b54\u677f\u4e2d\u904e\u591c\u3002<sup>\u5c07\u7d30\u80de\u8207 20 \u00b5L\/\u5b54\u7684\u6e2c\u5b9a\u6eb6\u6db2\u5728 37 o<\/sup>\u00a0C\u4e0b\u5b75\u80b2 3 \u5c0f\u6642\u3002\u5728 570 nm \u548c 605 nm \u8655\u6e2c\u91cf\u5438\u5149\u5ea6\u5f37\u5ea6\u3002\u5982\u5716\u6240\u793a\uff0cOD570\/OD605 \u7684\u6bd4\u7387\u8207\u7d30\u80de\u6578\u91cf\u6210\u6b63\u6bd4<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/06\/1-\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09-Abcam-\u8ca8\u865fab112118.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E7%9B%B8%E9%97%9C%E5%84%80%E5%99%A8-300x114.png\" alt=\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e5\u0084\u0080\u00e5\u0099\u00a8\" width=\"97\" height=\"38\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=19075\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%998-300x288.png\" alt=\"CUBEE\u684c\u4e0a\u578b\u5c0f\u578b\u96e2\u5fc3\u6a5f\" width=\"175\" height=\"167\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=18163\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%996-300x288.png\" alt=\"WPACO8000\" width=\"172\" height=\"170\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=6196\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%997-300x288.png\" alt=\"MUPID-2 \u6c34\u5e73\u96fb\u6cf3\u69fd\" width=\"172\" height=\"164\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=25739\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%993-300x288.png\" alt=\"BIODROP-\u5be6\u9a57\u8a2d\u50993\" width=\"173\" height=\"168\" \/><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%991-300x288.png\" alt=\"ELISA reader\" width=\"169\" height=\"160\" \/><\/a><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u6bd2\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09| Abcam \u8ca8\u865fab112118 Cell Cytotoxicity Assa [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5798],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42794"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42794"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42794\/revisions"}],"predecessor-version":[{"id":42796,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42794\/revisions\/42796"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42794"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}