{"id":4249,"date":"2015-01-26T16:28:14","date_gmt":"2015-01-26T08:28:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=4249"},"modified":"2017-05-24T14:58:57","modified_gmt":"2017-05-24T06:58:57","slug":"gamma-h2ax-pharmacodynamic-assay_trevigen","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=4249","title":{"rendered":"\u3010DNA damage\u3011gamma H2AX Pharmacodynamic Assay\uff0dTrevigen"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"line-height: 1.5em;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-large wp-image-943\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/trevigen_product_banner01-1024x145.jpg\" alt=\"Innova_product_banner01\" width=\"1024\" height=\"145\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/trevigen_product_banner01-1024x145.jpg 1024w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/trevigen_product_banner01-300x42.jpg 300w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/trevigen_product_banner01.jpg 1408w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><span style=\"color: #800080; line-height: 1.5em;\">\u3000\u3000\u7f8e\u570bTREVIGEN\u516c\u53f8\u6210\u7acb\u65bc1992\u5e74\u3002\u81f4\u529b\u65bc\u7814\u767c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3001DNA\u65b7\u640d\u8207\u4fee\u5fa9\u3001\u9ad4\u5167\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08In vivo angiogenesis assay, DIVAA\uff09\u3001\u9ad4\u5916\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08in vitro angiogenesis assay\uff09\u3001\u6167\u661f\u5206\u6790\u6cd5\uff08Comet assay Kit\uff09\u3001\u7d30\u80de\u79fb\u884c\/\u7d30\u80de\u4fb5\u8972\uff08Cell migration and Cell invasion assay\uff09\u3001\u57fa\u5e95\u819c\u8403\u53d6\u7269\uff08Basement Membrane Extract\uff09\u3001\u4ee5\u53caPAR\u3001PARP\u3001PARG\u6240\u9700\u7528\u5230\u7684\u6297\u9ad4\u8207\u8a66\u5291\u3002<\/span><\/span><\/p>\n<p><span style=\"color: #008000; font-size: 14pt;\"><strong>Trevigen\u00a0gamma H2AX Pharmacodynamic Assay<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000gamma H2AX \u662f\u4e00\u500b\u78f7\u9178\u5316\u7684Histone\uff0c\u8207double-strand DNA damage\u8207apoptosis\u9ad8\u5ea6\u95dc\u806f\u3002\u672c\u5957\u7d44\u5229\u7528\u5316\u5b78\u51b7\u5149\u6cd5\uff0c\u5f97\u4ee5\u5feb\u901f\u6aa2\u6e2cgamma H2AX\u5728peripheral blood mononuclear cells\uff08PBMC\uff09\u3001cultured cells\u3001\u8207tissue biopsies\u7684\u542b\u91cf\u3002<\/p>\n<ul>\n<li>\u5316\u5b78\u51b7\u5149\u6cd5ELISA\u5075\u6e2c\uff08\u975e\u653e\u5c04\u6027\uff09<\/li>\n<li>\u6297\u9ad4\u9810\u5438\u9644\u65bc96 well plate<\/li>\n<li>Dynamic range\uff1a10 pM\uff0d800pM<\/li>\n<li>Sensitivity\uff1a5 pM of gamma H2AX<\/li>\n<\/ul>\n<p><span style=\"color: #0000ff;\"><strong><a style=\"color: #0000ff;\" title=\"gamma H2AX calculation\" href=\"http:\/\/www.trevigen.com\/docs\/1393361376.4418-096-k_calculation_worksheet.xls\" target=\"_blank\">\u3010\u8a08\u7b97\u7528excel\u8868\u683c\u4e0b\u8f09\u3011<\/a><\/strong><\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"100\">\n<p style=\"text-align: center;\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n<td style=\"text-align: center;\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td width=\"100\">\n<p style=\"text-align: center;\"><strong>\u5305\u88dd<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">4418-096-K<\/td>\n<td width=\"476\">gamma H2AX Pharmacodynamic Assay<\/td>\n<td width=\"100\">\n<p style=\"text-align: center;\">96 tests<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"580\">\n<p style=\"text-align: center;\"><strong>\u95dc\u806f\u5546\u54c1<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">4250-050-K<\/td>\n<td width=\"476\">CometAssay Kit<\/td>\n<td style=\"text-align: center;\" width=\"100\">50 samples<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">4380-096-K<\/td>\n<td width=\"476\">8 oxodG ELISA kit II<\/td>\n<td style=\"text-align: center;\" width=\"100\">96 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">4118-APC-100<\/td>\n<td width=\"476\">Anti-Phosphorylated H2AX Affinity Purified Polyclonal<\/td>\n<td style=\"text-align: center;\" width=\"100\">100 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">4335-MC-100<\/td>\n<td width=\"476\">Anti-PAR Monoclonal Antibody<\/td>\n<td style=\"text-align: center;\" width=\"100\">100 \u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ul>\n<li><span style=\"font-size: 12pt; color: #008000;\"><strong>Citation\uff1a<\/strong><\/span>\n<ul>\n<li>Abstract 2423: Development of a validated high throughput ELISA assay for gamma H2AX as a pharmacodynamic marker.\u00a0Jay George and Hai Xu,\u00a0Cancer Res., Oct 2014; 74: 2423.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000\u7f8e\u570bTREVIGEN\u516c\u53f8\u6210\u7acb\u65bc1992\u5e74\u3002\u81f4\u529b\u65bc\u7814\u767c\u5075\u6e2c\u7d30\u80de\u51cb\u4ea1\u3001DNA\u65b7\u640d\u8207\u4fee\u5fa9\u3001\u9ad4\u5167\u8840\u7ba1\u65b0\u751f\u5206\u6790\u5957\u7d44\uff08 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4258,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[84,121,183],"tags":[287,286],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/4249"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4249"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/4249\/revisions"}],"predecessor-version":[{"id":4254,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/4249\/revisions\/4254"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4258"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}